AIdit-BRC06

LOCUS       AIdit-BRC06                7339 bp DNA     circular SYN 22-JUN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 22, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7339
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     17..39
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     31..48
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     32..48
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     58..77
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        58..76
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     105..121
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     106..122
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             177..2042
                     /codon_start=1
                     /label=AAV2-rep
                     /translation="MPGFYEIVIKVPSDLDEHLPGISDSFVNWVAEKEWELPPDSDMDL
                     NLIEQAPLTVAEKLQRDFLTEWRRVSKAPEALFFVQFEKGESYFHMHVLVETTGVKSMV
                     LGRFLSQIREKLIQRIYRGIEPTLPNWFAVTKTRNGAGGGNKVVDECYIPNYLLPKTQP
                     ELQWAWTNMEQYLSACLNLTERKRLVAQHLTHVSQTQEQNKENQNPNSDAPVIRSKTSA
                     RYMELVGWLVDKGITSEKQWIQEDQASYISFNAASNSRSQIKAALDNAGKIMSLTKTAP
                     DYLVGQQPVEDISSNRIYKILELNGYDPQYAASVFLGWATKKFGKRNTIWLFGPATTGK
                     TNIAEAIAHTVPFYGCVNWTNENFPFNDCVDKMVIWWEEGKMTAKVVESAKAILGGSKV
                     RVDQKCKSSAQIDPTPVIVTSNTNMCAVIDGNSTTFEHQQPLQDRMFKFELTRRLDHDF
                     GKVTKQEVKDFFRWAKDHVVEVEHEFYVKKGGAKKRPAPSDADISEPKRVRESVAQPST
                     SDAEASINYADRYQNKCSRHVGMNLMLFPCRQCERMNQNSNICFTHGQKDCLECFPVSE
                     SQPVSVVKKAYQKLCYIHHIMGKVPDACTACDLVNVDLDDCIFEQ"
     CDS             2059..4275
                     /codon_start=1
                     /label=AAV-capBRC06
                     /translation="MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGL
                     VLPGYKYLGPGNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLKYNHADAEFQERL
                     KEDTSFGGNLGRAVFQAKKRLLEPLGLVEEAAKTAPGKKRPVEQSPQEPDSSAGIGKSG
                     AQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPSGVGSLTMASGGGAPVADNNEGADGVG
                     SSSGNWHCDSQWLGDRVITTSTRTWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPW
                     GYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTDNNGVKTIANNLTS
                     TVQVFTDSDYQLPYVLGSAHEGCLPPFPADVFMIPQYGYLTLNDGSQAVGRSSFYCLEY
                     FPSQMLRTGNNFQFSYEFENVPFHSSYAHSQSLDRLMNPLIDQYLYYLSKTINGSGQNQ
                     QTLKFSVAGPSNMAVQGRNYIPGPSYRQQRVSTTVTQNNNSEFAWPGASSWALNGRNSL
                     MNPGPAMASHKEGEDRFFPLSGSLIFGKQGTGRDNVDADKVMITNEEEIKTTNPVATES
                     YGQVATNHQSGSPTFYSAQTGWVQNQGILPGMVWQDRDVYLQGPIWAKIPHTDGNFHPS
                     PLMGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQVSVEIEWELQKEN
                     SKRWNPEIQYTSNYYKSNNVEFAVNTEGVYSEPRPIGTRYLTRNL"
     primer_bind     complement(4583..4603)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4583..4601)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4622..4638)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4622..4638)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4635..4657)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4646..4662
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4670..4700)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4715..4736
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4853..4870)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5024..5612)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5104..5123)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5783..6643)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    5957..5962
                     /label=PolyAMotif
     primer_bind     6406..6425
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6644..6748)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    6726..6731
                     /label=PolyAMotif
     rep_origin      complement(6774..7229)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6911..6932)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7123..7142
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa
       61 tacgactcac tatagggcga attgggtacc gggccccccc tcgatcgagg tcgacggtat
      121 cgggggagct cgcagggtct ccattttgaa gcgggaggtt tgaacgcgca gccgccatgc
      181 cggggtttta cgagattgtg attaaggtcc ccagcgacct tgacgagcat ctgcccggca
      241 tttctgacag ctttgtgaac tgggtggccg agaaggaatg ggagttgccg ccagattctg
      301 acatggatct gaatctgatt gagcaggcac ccctgaccgt ggccgagaag ctgcagcgcg
      361 actttctgac ggaatggcgc cgtgtgagta aggccccgga ggctcttttc tttgtgcaat
      421 ttgagaaggg agagagctac ttccacatgc acgtgctcgt ggaaaccacc ggggtgaaat
      481 ccatggtttt gggacgtttc ctgagtcaga ttcgcgaaaa actgattcag agaatttacc
      541 gcgggatcga gccgactttg ccaaactggt tcgcggtcac aaagaccaga aatggcgccg
      601 gaggcgggaa caaggtggtg gatgagtgct acatccccaa ttacttgctc cccaaaaccc
      661 agcctgagct ccagtgggcg tggactaata tggaacagta tttaagcgcc tgtttgaatc
      721 tcacggagcg taaacggttg gtggcgcagc atctgacgca cgtgtcgcag acgcaggagc
      781 agaacaaaga gaatcagaat cccaattctg atgcgccggt gatcagatca aaaacttcag
      841 ccaggtacat ggagctggtc gggtggctcg tggacaaggg gattacctcg gagaagcagt
      901 ggatccagga ggaccaggcc tcatacatct ccttcaatgc ggcctccaac tcgcggtccc
      961 aaatcaaggc tgccttggac aatgcgggaa agattatgag cctgactaaa accgcccccg
     1021 actacctggt gggccagcag cccgtggagg acatttccag caatcggatt tataaaattt
     1081 tggaactaaa cgggtacgat ccccaatatg cggcttccgt ctttctggga tgggccacga
     1141 aaaagttcgg caagaggaac accatctggc tgtttgggcc tgcaactacc gggaagacca
     1201 acatcgcgga ggccatagcc cacactgtgc ccttctacgg gtgcgtaaac tggaccaatg
     1261 agaactttcc cttcaacgac tgtgtcgaca agatggtgat ctggtgggag gaggggaaga
     1321 tgaccgccaa ggtcgtggag tcggccaaag ccattctcgg aggaagcaag gtgcgcgtgg
     1381 accagaaatg caagtcctcg gcccagatag acccgactcc cgtgatcgtc acctccaaca
     1441 ccaacatgtg cgccgtgatt gacgggaact caacgacctt cgaacaccag cagccgttgc
     1501 aagaccggat gttcaaattt gaactcaccc gccgtctgga tcatgacttt gggaaggtca
     1561 ccaagcagga agtcaaagac tttttccggt gggcaaagga tcacgtggtt gaggtggagc
     1621 atgaattcta cgtcaaaaag ggtggagcca agaaaagacc cgcccccagt gacgcagata
     1681 taagtgagcc caaacgggtg cgcgagtcag ttgcgcagcc atcgacgtca gacgcggaag
     1741 cttcgatcaa ctacgcagac aggtaccaaa acaaatgttc tcgtcacgtg ggcatgaatc
     1801 tgatgctgtt tccctgcaga caatgcgaga gaatgaatca gaattcaaat atctgcttca
     1861 ctcacggaca gaaagactgt ttagagtgct ttcccgtgtc agaatctcaa cccgtttctg
     1921 tcgtcaaaaa ggcgtatcag aaactgtgct acattcatca tatcatggga aaggtgccag
     1981 acgcttgcac tgcctgcgat ctggtcaatg tggatttgga tgactgcatc tttgaacaat
     2041 aaatgattta aatcaggtat ggctgccgat ggttatcttc cagattggct cgaggacaac
     2101 cttagtgaag gaattcgcga gtggtgggct ttgaaacctg gagcccctca acccaaggca
     2161 aatcaacaac atcaagacaa cgctcgaggt cttgtgcttc cgggttacaa ataccttgga
     2221 cccggcaacg gactcgacaa gggggagccg gtcaacgcag cagacgcggc ggccctcgag
     2281 cacgacaagg cctacgacca gcagctcaag gccggagaca acccgtacct caagtacaac
     2341 cacgccgacg ccgagttcca ggagcggctc aaagaagata cgtcttttgg gggcaacctc
     2401 gggcgagcag tcttccaggc caaaaagagg cttcttgaac ctcttggtct ggttgaggaa
     2461 gcggctaaga cggctcctgg aaagaagagg cctgtagagc agtctcctca ggaaccggac
     2521 tcctccgcgg gtattggcaa atcgggtgca cagcccgcta aaaagagact caatttcggt
     2581 cagactggcg acacagagtc agtcccagac cctcaaccaa tcggagaacc tcccgcagcc
     2641 ccctcaggtg tgggatctct tacaatggct tcaggtggtg gcgcaccagt ggcagacaat
     2701 aacgaaggtg ccgatggagt gggtagttcc tcgggaaatt ggcattgcga ttcccaatgg
     2761 ctgggggaca gagtcatcac caccagcacc cgaacctggg ccctgcccac ctacaacaat
     2821 cacctctaca agcaaatctc caacagcaca tctggaggat cttcaaatga caacgcctac
     2881 ttcggctaca gcaccccctg ggggtatttt gacttcaaca gattccactg ccacttctca
     2941 ccacgtgact ggcagcgact catcaacaac aactggggat tccggcctaa gcgactcaac
     3001 ttcaagctct tcaacattca ggtcaaagag gttacggaca acaatggagt caagaccatc
     3061 gccaataacc ttaccagcac ggtccaggtc ttcacggact cagactatca gctcccgtac
     3121 gtgctcgggt cggctcacga gggctgcctc ccgccgttcc cagcggacgt tttcatgatt
     3181 cctcagtacg ggtatctgac gcttaatgat ggaagccagg ccgtgggtcg ttcgtccttt
     3241 tactgcctgg aatatttccc gtcgcaaatg ctaagaacgg gtaacaactt ccagttcagc
     3301 tacgagtttg agaacgtacc tttccatagc agctacgctc acagccaaag cctggaccga
     3361 ctaatgaatc cactcatcga ccaatacttg tactatctct caaagactat taacggttct
     3421 ggacagaatc aacaaacgct aaaattcagt gtggccggac ccagcaacat ggctgtccag
     3481 ggaagaaact acatacctgg acccagctac cgacaacaac gtgtctcaac cactgtgact
     3541 caaaacaaca acagcgaatt tgcttggcct ggagcttctt cttgggctct caatggacgt
     3601 aatagcttga tgaatcctgg acctgctatg gccagccaca aagaaggaga ggaccgtttc
     3661 tttcctttgt ctggatcttt aatttttggc aaacaaggaa ctggaagaga caacgtggat
     3721 gcggacaaag tcatgataac caacgaagaa gaaattaaaa ctactaaccc ggtagcaacg
     3781 gagtcctatg gacaagtggc cacaaaccac cagagtggtt ctcctacgtt ttatagtgcg
     3841 cagaccggct gggttcaaaa ccaaggaata cttccgggta tggtttggca ggacagagat
     3901 gtgtacctgc aaggacccat ttgggccaaa attcctcaca cggacggcaa ctttcaccct
     3961 tctccgctga tgggagggtt tggaatgaag cacccgcctc ctcagatcct catcaaaaac
     4021 acacctgtac ctgcggatcc tccaacggcc ttcaacaagg acaagctgaa ctctttcatc
     4081 acccagtatt ctactggcca agtcagcgtg gagatcgagt gggagctgca gaaggaaaac
     4141 agcaagcgct ggaacccgga gatccagtac acttccaact attacaagtc taataatgtt
     4201 gaatttgctg ttaatactga aggtgtatat agtgaacccc gccccattgg caccagatac
     4261 ctgactcgta atctgtaatt gcttgttaat caataaaccg tttaattcgt ttcagttgaa
     4321 ctttggtctc tgcgaagggc gaattcgttt aaacctgcag gactagaggt cctgtattag
     4381 aggtcacgtg agtgttttgc gacattttgc gacaccatgt ggtcacgctg ggtatttaag
     4441 cccgagtgag cacgcagggt ctccattttg aagcgggagg tttgaacgcg cagccgccaa
     4501 gccgaattct gcagatatcc atcacactgg cggccgctcg actagagcgg ccgccaccgc
     4561 ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
     4621 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     4681 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     4741 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     4801 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     4861 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     4921 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4981 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     5041 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     5101 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     5161 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     5221 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     5281 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     5341 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     5401 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     5461 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     5521 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     5581 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     5641 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     5701 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     5761 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     5821 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     5881 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     5941 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     6001 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     6061 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     6121 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     6181 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     6241 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     6301 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     6361 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     6421 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     6481 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     6541 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     6601 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     6661 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     6721 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt
     6781 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     6841 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     6901 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     6961 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     7021 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     7081 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     7141 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     7201 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg
     7261 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     7321 tgcaaggcga ttaagttgg
//