pHR-Tre3G-29xGGGGCC-12xMS2

LOCUS       pHR-Tre3G-29xGGGGCC-12xMS2                9790 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Encodes 29xGGGGCC repeats with 12xMS2 tag, 2nd generation lentiviral
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    pHR-Tre3G-29xGGGGCC-12xMS2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9790)
  AUTHORS   Jain A, Vale RD
  TITLE     RNA phase transitions in repeat expansion disorders.
  JOURNAL   Nature. 2017 Jun 8;546(7657):243-247. doi: 10.1038/nature22386. Epub
            2017 May 31.
  PUBMED    28562589
REFERENCE   2  (bases 1 to 9790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9790
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(1..18)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(308..327)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(541..560)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     660..682
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(720..738)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        806..910
                     /gene="bla"
                     /label=AmpR promoter
     CDS             911..1771
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1129..1148)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1942..2530
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2431..2450
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2684..2701
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2771..2791)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2776..3105
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2956..3091
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3018..3037
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     intron          4239..4304
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4434..4454
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4879..5013
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4916..4935)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4970..4989
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5182..5815
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5862..5987
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6484..6716
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6901..6945
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7230..7347
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7409..7758
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    7417..7435
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7453..7471
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7489..7507
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7525..7543
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7561..7579
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7597..7615
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7633..7651
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     7697..7721
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_RNA        8126..8144
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        8165..8183
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        8235..8253
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        8274..8292
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        8344..8362
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        8383..8401
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        8453..8471
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        8492..8510
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        8562..8580
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        8601..8619
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        8671..8689
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        8710..8728
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_feature    8834..9422
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8887..8907)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9305..9316)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9425..9441)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9426..9442)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9532..9765
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(9790..18)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 ctatagtgtc acctaaatcg tatgtgtatg atacataagg ttatgtatta attgtagccg
       61 cgttctaacg acaatatgta caagcctaat tgtgtagcat ctggcttact gaagcagacc
      121 ctatcatctc tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt
      181 tctggtaacg ccgtcccgca cccggaaatg gtcagcgaac caatcagcag ggtcatcgct
      241 agccagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
      301 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
      361 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
      421 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
      481 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaatggt gcactctcag
      541 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
      601 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
      661 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
      721 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
      781 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
      841 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      901 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      961 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     1021 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     1081 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     1141 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     1201 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     1261 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     1321 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     1381 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     1441 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     1501 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     1561 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     1621 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     1681 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     1741 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1801 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1861 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1921 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1981 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     2041 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtct ttctagtgta
     2101 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     2161 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     2221 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     2281 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     2341 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     2401 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     2461 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     2521 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     2581 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     2641 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     2701 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     2761 atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     2821 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     2881 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     2941 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     3001 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     3061 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tggacacaag
     3121 acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc
     3181 gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct
     3241 cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact
     3301 tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact
     3361 cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg
     3421 tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt
     3481 atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc
     3541 gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac tgcggttgcg
     3601 attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt
     3661 ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggattga acagccgtgg
     3721 gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca
     3781 ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttccag taagtattct
     3841 ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa
     3901 acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca
     3961 gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg
     4021 gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag
     4081 cgatgccgaa cgtaccgacg atgatttata cgatacggtg attggctacc gtggcggcaa
     4141 ctggatttat gagtgggccc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat
     4201 aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg
     4261 tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa
     4321 ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag
     4381 aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa
     4441 agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc
     4501 atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag
     4561 ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata
     4621 acagttataa tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta
     4681 ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata
     4741 aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta
     4801 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     4861 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     4921 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     4981 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
     5041 catcccaaac ttcccacccc ataccctatt accactgcca attacctagt ggtttcattt
     5101 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
     5161 atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc
     5221 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
     5281 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     5341 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
     5401 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
     5461 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
     5521 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
     5581 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
     5641 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     5701 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     5761 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     5821 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     5881 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     5941 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     6001 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     6061 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     6121 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     6181 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     6241 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     6301 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     6361 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     6421 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     6481 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     6541 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     6601 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     6661 gcagctccag gcaagaatcc tggctgtgaa agatacctaa aggatcaaca gctcctgggg
     6721 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     6781 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     6841 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     6901 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     6961 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     7021 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     7081 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     7141 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     7201 tatcgccaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     7261 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     7321 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
     7381 tatcgatgag gccctttcgt cttcactcga gtttactccc tatcagtgat agagaacgta
     7441 tgaagagttt actccctatc agtgatagag aacgtatgca gactttactc cctatcagtg
     7501 atagagaacg tataaggagt ttactcccta tcagtgatag agaacgtatg accagtttac
     7561 tccctatcag tgatagagaa cgtatctaca gtttactccc tatcagtgat agagaacgta
     7621 tatccagttt actccctatc agtgatagag aacgtatgtc gaggtaggcg tgtacggtgg
     7681 gcgcctataa aagcagagct cgtttagtga accgtcagat cgcctggagc aattccacaa
     7741 cacttttgtc ttatacttac gcgtgtcgac catatgacta gtatgggtaa ttataacccg
     7801 ggccctatat attaggctaa ctagctaact aggaattcat atcaagcttt agctagctaa
     7861 ctagctaact aggctcttcc ggccggggcc ggggccgggg ccggggccgg ggccggggcc
     7921 ggggccgggg ccggggccgg ggccggggcc ggggccgggg ccggggccgg ggccggggcc
     7981 ggggccgggg ccggggccgg ggccggggcc ggggccgggg ccggggccgg ggccggggcc
     8041 ggggccgggg ccggggcctc tcgagtctag agggccgcaa gcttggtacc gagctcggat
     8101 cctaaggtac ctaattgcct agaaaacatg aggatcaccc atgtctgcag gtcgactcta
     8161 gaaaacatga ggatcaccca tgtctgcagt attcccgggt tcattagatc ctaaggtacc
     8221 taattgccta gaaaacatga ggatcaccca tgtctgcagg tcgactctag aaaacatgag
     8281 gatcacccat gtctgcagta ttcccgggtt cattagatcc taaggtacct aattgcctag
     8341 aaaacatgag gatcacccat gtctgcaggt cgactctaga aaacatgagg atcacccatg
     8401 tctgcagtat tcccgggttc attagatcct aaggtaccta attgcctaga aaacatgagg
     8461 atcacccatg tctgcaggtc gactctagaa aacatgagga tcacccatgt ctgcagtatt
     8521 cccgggttca ttagatccta aggtacctaa ttgcctagaa aacatgagga tcacccatgt
     8581 ctgcaggtcg actctagaaa acatgaggat cacccatgtc tgcagtattc ccgggttcat
     8641 tagatcctaa ggtacctaat tgcctagaaa acatgaggat cacccatgtc tgcaggtcga
     8701 ctctagaaaa catgaggatc acccatgtct gcagtattcc cgggttcatt agatctgcgc
     8761 gcgatcgata tcagcgcttt aaatttgcgc atgctagtgt aaggcatgca agcttgatat
     8821 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     8881 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     8941 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     9001 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     9061 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     9121 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     9181 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     9241 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     9301 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     9361 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     9421 gcatcgatac cgtcgacctc gagggaatta attcgagctc ggtaccttta agaccaatga
     9481 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     9541 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     9601 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     9661 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     9721 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagcatc tagaattaat
     9781 tccgtgtatt
//