pAAV-cFos1-mAvicFP1-iPAK4

LOCUS       pAAV-cFos1-mAvicFP1-iPAK4              7569 bp DNA     circular SYN 23-APR-2024
DEFINITION  Express FlicR1 preferentially in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7569)
  AUTHORS   Abdelfattah AS, Farhi SL, Zhao Y, Brinks D, Zou P, Ruangkittisakul 
            A, Platisa J, Pieribone VA, Ballanyi K, Cohen AE, Campbell RE
  TITLE     A Bright and Fast Red Fluorescent Protein Voltage Indicator That 
            Reports Neuronal Activity in Organotypic Brain Slices.
  JOURNAL   J Neurosci. 2016 Feb 24;36(8):2458-72. doi: 
            10.1523/JNEUROSCI.3484-15.2016.
  PUBMED    26911693
REFERENCE   2  (bases 1 to 7569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 14, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.3484-15"; journalName: "J Neurosci"; date: 
            "2016-02-24- 24"; volume: "36"; issue: "8"; pages: "2458-72"
FEATURES             Location/Qualifiers
     source          1..7569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          577..2253
                     /lab_host="."
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          2990..4066
                     /mol_type="genomic DNA"
                     /organism="."
     protein_bind    229..250
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        265..295
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    303..319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     308..330
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     327..343
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     327..343
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   381..510
                     /label=AAV2 ITR
     promoter        577..2253
                     /label=putative_fos
                     /note="/created_by=xiuyuanli
                     https://www.ncbi.nlm.nih.gov/nucleotide/V00727.1?report=gen
                     bank&log$=nuclalign&blast_rank=3&RID=55DC32WT016: 1-1618"
     promoter        873..1190
                     /label=putative_fos_from34856
                     /note="/created_by=xiuyuanli"
                     /note="/modified_by=xiuyuanli"
     misc_feature    1338..1345
                     /label=Fos oncogene start
     primer_bind     complement(2230..2275)
                     /label=MCS-cfos-R
     primer_bind     2268..2293
                     /label=pUBC-mChilada-f
     CDS             2273..2989
                     /codon_start=1
                     /product="https://www.addgene.org/browse/article/28203833/"
                     /label=mAvicFP1
                     /translation="MVSKGEELFNGIVPILIELNGDVHGHKFSVRGEGEGDAGSGKIEI
                     KFVCTTGTLPVPWPTLVTTLCYGVQCFTRYPEHMKQHDFYKSAMPDGYIQKRTISFQDD
                     GHYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGNKMEYNYNSHSVYVLSDKANNGIK
                     VNFKIRHNLKGEGIQLADHDQQNIPIGDGPVLLPDYHYLSTQTKITKDPNEKRDHMNLV
                     EFVTACGITHGMDELYK"
     regulatory      2273..2276
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     2969..3015
                     /label=GFP-iPAK4-F
     primer_bind     2969..3015
                     /label=Primer 1a
     CDS             3002..3115
                     /codon_start=1
                     /label=INKA-box
                     /translation="EAEDWTAALLNRGRSRQPLVLGDNCFADLVHNWMELPE"
     CDS             3122..4063
                     /codon_start=1
                     /label=Pak4cat
                     /translation="PAAPAVPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLD
                     NFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEM
                     YNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRD
                     IKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIW
                     SLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPA
                     QRATAAELLKHPFLAKAGPPASIVPLMRQNRTR"
     primer_bind     complement(3215..3233)
                     /label=PAK4-R
     primer_bind     complement(4046..4088)
                     /label=HindIII-PAK4-R
     misc_feature    4079..4667
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4132..4152)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4550..4561)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4670..4686)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4671..4687)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    4716..4837
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4727..4746)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4781..4800
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4819..4837)
                     /label=SV40-polyA
     repeat_region   4999..5128
                     /label=AAV2 ITR
     primer_bind     complement(5147..5164)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5147..5163)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5156..5178)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5305..5760
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5392..5411)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5602..5623
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5786..5890
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5891..6751
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6109..6128)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6922..7510
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7411..7430
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
       61 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      121 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
      181 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
      241 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
      301 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccagatt
      361 taattaaggc cttaattagg ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc
      421 ccgggcgtcg ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg
      481 gagtggccaa ctccatcact aggggttcct tgtagttaat gattaacccg ccatgctact
      541 tatctacgta gccatgctct aggaagatct ctgcagaagc tttcctttag gaacagaggc
      601 ttcgagcctt taaggctgcg tacttgcttc tcctaatacc agagactcaa aaaaaaaaaa
      661 aaagttccag attgctggac aatgacccgg gtctcatccc ttgaccctgg gaaccgggtc
      721 cacattgaat caggtgcgaa tgttcgctcg ccttctctgc ctttcccgcc tcccctcccc
      781 cggccgcggc cccggttccc cccctgcgct gcaccctcag agttggctgc agccggcgag
      841 ctgttcccgt caatccctcc ctcctttaca caggatgtcc atattaggac atctgcgtca
      901 gcaggtttcc acggccggtc cctgttgttc tggggggggg accatctccg aaatcctaca
      961 cgcggaaggt ctaggagacc ccctaagatc ccaaatgtga acactcatag gtgaaagatg
     1021 tatgccaaga cgggggttga aagcctgggg cgtagagttg acgacagagc gcccgcagag
     1081 ggccttgggg cgcgcttccc cccccttcca gttccgccca gtgacgtagg aagtccatcc
     1141 attcacagcg cttctataaa ggcgccagct gaggcgccta ctactccaac cgcgactgca
     1201 gcgagcaact gagaagactg gatagagccg gcggttccgc gaacgagcag tgaccgcgct
     1261 cccacccagc tctgctctgc agctcccacc agtgtctacc cctggacccc ttgccgggct
     1321 ttccccaaac ttcgaccatg atgttctcgg gtttcaacgc cgactacgag gcgtcatcct
     1381 cccgctgcag tagcgcctcc ccggccgggg acagcctttc ctactaccat tccccagccg
     1441 actccttctc cagcatgggc tctcctgtca acacacaggt gagtttggct ttgtgtagcc
     1501 gccaggtccg cgctgagggt cgccgtggag gagacactgg ggtgtgactc gcaggggcgg
     1561 gggggtcttc ctttttcgct ctggagggag actggcgcgg tcagagcagc cttagcctgg
     1621 gaacccagga cttgtctgag cgcgtgcaca cttgtcatag taagacttag tgaccccttc
     1681 ccgcgcggca ggtttattct gagtggcctg cctgcattct tctctcggcc gacttgtttc
     1741 tgagatcagc cggggccaac aagtctcgag caaagagtcg ctaactagag tttgggaggc
     1801 ggcaaaccgc ggcaatcccc cctcccgggg cagcctggag cagggaggag ggaggaggga
     1861 ggagggtgct gcgggcgggt gtgtaaggca gtttcattga taaaaagcga gttcattctg
     1921 gagactccgg agcagcgcct gcgtcagcgc agacgtcagg gatatttata acaaaccccc
     1981 tttcgagcga gtgatgccga agggataacg ggaacgcagc agtaggatgg aggggaaagg
     2041 ctgcgctgcg gaattcaagg gaggatattg ggagagcttt tatctccgat gaggtgcata
     2101 caggaagaca taagcagtct ctgaccggaa tgcttctctc tccctgcttc atgcgacact
     2161 agggccactt gctccacctg tgtctggaac ctcctcgctc acctccgctt tcctcttttt
     2221 gttttgtttc aggacttttg cgcagatctc tcctcgaatt cggggatcca ccatggtgag
     2281 caagggcgag gagctgttca acggtatcgt tcctatcctg atcgaactga acggtgacgt
     2341 tcatggtcat aagttctctg ttcgtggtga aggtgaaggt gacgctggtt ctggtaagat
     2401 cgaaatcaag ttcgtttgca ccaccggtac cctgcctgtt ccttggccta ccctggttac
     2461 caccttatgc tacggtgttc agtgcttcac ccgttaccct gaacatatga agcagcatga
     2521 cttctacaag tctgctatgc ctgacggtta catccagaag cgtaccatct ctttccagga
     2581 cgacggtcat tacaagaccc gtgctgaagt taagttcgaa ggtgacaccc tggttaaccg
     2641 tatcgaactg aagggtatcg acttcaagga agacggtaac atcctgggta acaagatgga
     2701 atacaactac aactctcatt ctgtttacgt tctgtctgac aaggctaaca acggtatcaa
     2761 ggttaacttc aagatccgtc ataacctgaa gggtgaaggt atccagctgg ctgaccatga
     2821 ccagcagaac atccctatcg gtgacggtcc tgttctgctg cctgactacc attacctgtc
     2881 tacccagacc aagatcacca aggaccctaa cgaaaagcgt gaccatatga acctggttga
     2941 attcgttacc gcttgcggta tcacccatgg catggacgag ctgtacaagt caggtggatc
     3001 cgaagcagag gactggacag cagccctact gaacaggggt cgcagtcgcc agcccctggt
     3061 actaggggac aattgctttg ctgacttggt gcacaactgg atggagctgc ctgaggaatt
     3121 ccccgccgcc cctgctgttc ctgggccccc tggcccccgc tcaccacagc gggagccaca
     3181 gcgagtatcc catgagcagt tccgggctgc cctgcagctg gtggtggacc caggcgaccc
     3241 ccgctcctac ctggacaact tcatcaagat tggcgagggc tccacgggca tcgtgtgcat
     3301 cgccaccgtg cgcagctcgg gcaagctggt ggccgtcaag aagatggacc tgcgcaagca
     3361 gcagaggcgc gagctgctct tcaacgaggt ggtaatcatg agggactacc agcacgagaa
     3421 tgtggtggag atgtacaaca gctacctggt gggggacgag ctctgggtgg tcatggagtt
     3481 cctggaagga ggcgccctca ccgacatcgt cacccacacc aggatgaacg aggagcagat
     3541 cgcagccgtg tgccttgcag tgctgcaggc cctgtcggtg ctccacgccc agggcgtcat
     3601 ccaccgggac atcaagagcg actcgatcct gctgacccat gatggcaggg tgaagctgtc
     3661 agactttggg ttctgcgccc aggtgagcaa ggaagtgccc cgaaggaagt cgctggtcgg
     3721 cacgccctac tggatggccc cagagctcat ctcccgcctt ccctacgggc cagaggtaga
     3781 catctggtcg ctggggataa tggtgattga gatggtggac ggagagcccc cctacttcaa
     3841 cgagccaccc ctcaaagcca tgaagatgat tcgggacaac ctgccacccc gactgaagaa
     3901 cctgcacaag gtgtcgccat ccctgaaggg cttcctggac cgcctgctgg tgcgagaccc
     3961 tgcccagcgg gccacggcag ccgagctgct gaagcaccca ttcctggcca aggcagggcc
     4021 gcctgccagc atcgtgcccc tcatgcgcca gaaccgcacc agatgaaagc ttatcgataa
     4081 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     4141 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     4201 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     4261 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     4321 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     4381 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     4441 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     4501 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     4561 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     4621 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     4681 acctcgaccc gggcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca
     4741 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     4801 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     4861 tatgtttcag gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa
     4921 atgtggtaaa atcgataagg atcttcctag agcatggcta cgtagataag tagcatggcg
     4981 ggttaatcat taactacaag gaacccctag tgatggagtt ggccactccc tctctgcgcg
     5041 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg
     5101 cggcctcagt gagcgagcga gcgcgcagcc ttaattaacc taattcactg gccgtcgttt
     5161 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     5221 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     5281 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     5341 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     5401 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     5461 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     5521 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     5581 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     5641 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     5701 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     5761 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     5821 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5881 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     5941 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     6001 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     6061 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     6121 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     6181 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     6241 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     6301 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     6361 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     6421 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     6481 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     6541 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     6601 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     6661 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     6721 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     6781 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     6841 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6901 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6961 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     7021 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     7081 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     7141 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     7201 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     7261 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     7321 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     7381 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     7441 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     7501 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     7561 tgctcacat
//