| 质粒名称: | pAAVLINK-3'-CIC-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001694 |
| 产品用途: | 3' AAVLINK plasmid for CIC expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | Promoterless |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CIC |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-3'-CIC-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-3'-CIC-FLAG载体质粒基本信息
pAAVLINK-3'-CIC-FLAG质粒图谱载体图谱和pAAVLINK-3'-CIC-FLAG载体序列质粒序列多克隆位点信息
pAAVLINK-3'-CIC-FLAG质粒载体简介
pAAVLINK-3'-CIC-FLAG质粒序列载体序列
LOCUS pAAVLINK-3'-CIC-FLAG 7386 bp DNA circular SYN 20-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7386)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 2, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7386
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
protein_bind 145..178
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 179..270
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 271..4326
/codon_start=1
/label=CIC
/translation="VKEAHFKAHPDWKWCNKDRKKSSSEAKPTSLGLAGGHKETRERSM
SETGTAAAPGVSSELLSVAAQTLLSSDTKAPGSSSCGAERLHTVGGPGSARPRAFSHSG
VHSLDGGEVDSQALQELTQMVSGPASYSGPKPSTQYGAPGPFAAPGEGGALAATGRPPL
LPTRASRSQRAASEDMTSDEERMVICEEEGDDDVIADDGFGTTDIDLKCKERVTDSESG
DSSGEDPEGNKGFGRKVFSPVIRSSFTHCRPPLDPEPPGPPDPPVAFGKGYGSAPSSSA
SSPASSSASAATSFSLGSGTFKAQESGQGSTAGPLRPPPPGAGGPATPSKATRFLPMDP
ATFRRKRPESVGGLEPPGPSVIAAPPSGGGNILQTLVLPPNKEEQEGGGARVPSAPAPS
LAYGAPAAPLSRPAATMVTNVVRPVSSTPVPIASKPFPTSGRAEASPNDTAGARTEMGT
GSRVPGGSPLGVSLVYSDKKSAAATSPAPHLVAGPLLGTVGKAPATVTNLLVGTPGYGA
PAPPAVQFIAQGAPGGGTTAGSGAGAGSGPNGPVPLGILQPGALGKAGGITQVQYILPT
LPQQLQVAPAPAPAPGTKAAAPSGPAPTTSIRFTLPPGTSTNGKVLAATAPTPGIPILQ
SVPSAPPPKAQSVSPVQAPPPGGSAQLLPGKVLVPLAAPSMSVRGGGAGQPLPLVSPPF
SVPVQNGAQPPSKIIQLTPVPVSTPSGLVPPLSPATLPGPTSQPQKVLLPSSTRITYVQ
SAGGHALPLGTSPASSQAGTVTSYGPTSSVALGFTSLGPSGPAFVQPLLSAGQAPLLAP
GQVGVSPVPSPQLPPACAAPGGPVITAFYSGSPAPTSSAPLAQPSQAPPSLVYTVATST
TPPAATILPKGPPAPATATPAPTSPFPSATAGSMTYSLVAPKAQRPSPKAPQKVKAAIA
SIPVGSFEAGASGRPGPAPRQPLEPGPVREPTAPESELEGQPTPPAPPPLPETWTPTAR
SSPPLPPPAEERTSAKGPETMASKFPSSSSDWRVPGQGLENRGEPPTPPSPAPAPAVAP
GGSSESSSGRAAGDTPERKEAAGTGKKVKVRPPPLKKTFDSVDKVLSEVDFEERFAELP
EFRPEEVLPSPTLQSLATSPRAILGSYRKKRKNSTDLDSAPEDPTSPKRKMRRRSSCSS
EPNTPKSAKCEGDIFTFDRTGTEAEDVLGELEYDKVPYSSLRRTLDQRRALVMQLFQDH
GFFPSAQATAAFQARYADIFPSKVCLQLKIREVRQKIMQAATPTEQPPGAEAPLPVPPP
TGTAAAPAPTPSPAGGPDPTSPSSDSGTAQAAPPLPPPPESGPGQPGWEGAPQPSPPPP
GPSTAATGR"
misc_feature 275..280
/label=SDS
misc_feature 372..377
/label=SDS
misc_feature 839..844
/label=SDS
misc_feature 1007..1012
/label=SDS
misc_feature 1063..1068
/label=SDS
misc_feature 1103..1108
/label=SDS
misc_feature 1127..1132
/label=SDS
misc_feature 1148..1153
/label=SDS
misc_feature 1374..1379
/label=SDS
misc_feature 1415..1420
/label=SDS
regulatory 1510..1519
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
misc_feature 2501..2506
/label=SDS
CDS complement(2547..2564)
/codon_start=1
/product="thrombin recognition and cleavage site"
/label=thrombin site
/translation="LVPRGS"
misc_feature 2813..2818
/label=SDS
misc_feature 3470..3475
/label=SDS
misc_feature 3605..3610
/label=SDS
CDS 4333..4398
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
polyA_signal 4402..4626
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 4649..4789
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4649..4778
/label=AAV2 ITR
rep_origin 4864..5319
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5601..5705
/gene="bla"
/label=AmpR promoter
CDS 5706..6566
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6737..7325
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtataact tcgtatagga tactttatag caatttatga
181 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
241 ctgacatcca ctttgccttt ctctccacag gtgaaggagg cccacttcaa ggcccaccca
301 gattggaagt ggtgcaacaa ggaccgaaag aagtccagct cagaggccaa gcccacgagc
361 ctggggctgg caggagggca caaggagacg cgggagcgga gcatgtcgga gacgggcact
421 gctgctgccc ctggggtgtc ctctgagctc ctgtccgttg cagcccagac actcctgagc
481 tcagacacca aggctccggg gagcagctcc tgtggggcag aacggctaca cacagttggg
541 ggacctggct cagcccggcc ccgagctttc tcccacagcg gggtacacag cctggacggc
601 ggagaagtag acagtcaggc gctacaggaa ctgacgcaga tggtgtctgg ccctgcatcg
661 tactctggcc caaagccttc tacccagtat ggagctccag gaccctttgc agcccctggt
721 gagggaggtg ccttggcggc cactgggcgg cccccgctgc tgcccacccg agcttctcgt
781 tctcagcgtg cggccagtga ggacatgacg agtgatgagg agcgcatggt catctgtgag
841 gaggaagggg atgatgatgt cattgctgac gatggcttcg gcaccactga cattgatctc
901 aagtgcaagg agcgggtgac cgacagcgag agtggggaca gctctgggga ggacccagag
961 ggcaacaagg gctttggtcg gaaggtgttt tcacctgtga tccgttcctc ctttacccac
1021 tgccgccccc cactggaccc tgagccccca gggcccccgg atcctcctgt agcctttggc
1081 aaaggctatg gttccgcccc atcctcctct gcgtcctcgc ctgcttcctc ctcagcctcg
1141 gcagccacct ccttctcact gggctcagga accttcaagg cccaggagtc tggtcagggc
1201 agcacagcgg gccccctacg gcccccaccc cctggggctg ggggtccagc gacaccttcc
1261 aaggcaaccc ggttcctccc aatggatcct gccaccttcc ggcgcaagag acccgaaagt
1321 gtgggtggcc tggagccacc aggcccctca gtcatcgcgg cccctcccag cggaggagga
1381 aacatcctgc agacactggt gctgccccca aacaaggagg agcaagaggg cggcggagcc
1441 agagtgccct ccgcccccgc cccatcactg gcctatgggg ccccagcagc tcccctgtcc
1501 cgtcctgccg ccaccatggt caccaatgtg gtgcggcctg tcagcagcac tcctgtgccc
1561 atcgcctcta agcccttccc cacctctggc cgggctgagg cgtctccaaa tgacacagca
1621 ggtgccagga ctgaaatggg cactgggtct cgggtgcctg ggggctcccc gctgggtgtc
1681 agcttagtgt attcggacaa gaagtcggca gcagccacct caccagcccc acacttggtg
1741 gctggacccc tgctgggcac tgtggggaag gcgcctgcca ctgtcactaa cctactggtg
1801 ggcaccccgg ggtatggggc ccctgcgccc cctgctgtcc agttcattgc ccagggggcc
1861 cctggtggtg ggaccactgc gggctcagga gcaggtgctg ggagtggccc caatgggcca
1921 gtacccctgg gcatcctgca accaggtgcc ctgggcaagg ctgggggaat cacccaggta
1981 cagtacatcc tgcccacgct gccccagcag cttcaggtgg cacctgcccc agcaccagcc
2041 cctgggacca aggcagcggc tcccagcggc cctgcaccca ccaccagcat ccgtttcacc
2101 ctcccaccgg gcacttccac caacggcaaa gtcctggctg ccactgcacc cactcctggc
2161 atccccatcc tgcagtctgt accctccgcc ccacccccca aagcccagtc agtttctccc
2221 gtgcaggccc cgcccccggg tggctcagcc cagctgctgc ctgggaaggt cctagtgcct
2281 ctggccgccc ctagcatgtc agtgcggggt ggaggggccg gccagccact gccactggtg
2341 agcccgccct tctcagtacc tgtgcagaat ggtgcccagc cccccagcaa gatcatccag
2401 ctgaccccgg tgcctgtgag cacacccagc ggcctggtgc cgcccctgag cccagccaca
2461 ctccctggac ccacctctca gcctcagaag gtcctgttgc cctcctccac cagaatcacc
2521 tatgtgcagt cagcgggcgg gcacgcgctg cccctgggta ccagccctgc gtccagccag
2581 gctggaacag tcacctcgta cgggcccacg agctctgtag ctctaggctt cacctcgctg
2641 gggcccagcg gccccgcctt cgtgcagccc ctgctctcag caggccaagc cccactgctg
2701 gctcccggtc aggtgggcgt gtcacctgtg cccagtcccc agctgccgcc tgcctgtgca
2761 gcccccggag gtcctgtcat aacagcattt tactctggca gccctgcacc cacctcctca
2821 gcacccctgg cccagccatc ccaggccccc ccaagcctgg tctacactgt ggccaccagc
2881 acaaccccac ctgcagccac cattctgccc aagggcccgc cagcccctgc cactgccacc
2941 ccagccccga ctagcccttt ccccagcgcc acagcaggtt ccatgaccta cagcttagtg
3001 gcccccaagg cccagcggcc cagcccgaag gccccccaga aagtgaaggc agccatcgcc
3061 agcattcccg tggggtcctt tgaggcaggt gcctctgggc ggcctggccc tgcaccccgg
3121 cagcctctgg agcctggccc agtccgagag ccaactgccc cagagtctga gcttgagggg
3181 cagcccacac caccagcccc tccacccctg ccagagacct ggactcccac ggcccggagc
3241 agccccccac tgcccccacc tgctgaggag cggaccagcg ccaagggccc tgagaccatg
3301 gccagcaaat tccccagctc atcttcagac tggcgcgtcc ctgggcaggg cctggagaat
3361 cgtggggagc ctcccactcc tcccagcccg gccccagctc cagctgtagc ccctggtggc
3421 agcagcgaga gcagcagtgg gcgggcagcc ggggacaccc cggagcgcaa ggaggcggct
3481 ggtactggca agaaggtgaa ggtgcggccc ccgcccctga agaagacctt tgactctgtg
3541 gacaaggtcc tgtcagaagt ggacttcgaa gagcgctttg ctgagttgcc tgagtttcgg
3601 cctgaggagg tgctgccctc ccccaccctg cagtctctgg ccacctcacc ccgggccatc
3661 ctgggctctt accgcaagaa gaggaagaac tccacggacc tggattcagc acccgaggac
3721 cccacctcgc ccaagcgcaa gatgagaaga cgctccagct gcagctcgga gcccaacacc
3781 cccaagagtg ccaagtgcga gggggacatc ttcacctttg accgtacagg tacagaagcc
3841 gaggacgtgc ttggggagct agagtatgac aaggtgccat actcctccct gcggcgcacc
3901 ctggaccagc gccgggccct ggtcatgcag ctctttcagg accatggctt cttcccgtca
3961 gcccaggcca cagccgcctt ccaggcccgc tatgcagaca tctttccctc caaggtttgt
4021 ctgcagttga agatccgtga ggtgcgccag aagatcatgc aggctgccac tcccacggag
4081 cagccccctg gagctgaggc tcctctccct gtaccgcccc ccactggcac cgctgctgcc
4141 cctgccccca ctcccagccc cgcagggggc cctgacccca cctcacccag ctcggactct
4201 ggcacggccc aggctgcccc gccactgcct ccacccccag agtcggggcc tggacagcct
4261 ggctgggagg gggctcccca gccctccccc ccacccccag gtccctccac agctgccaca
4321 ggcaggtgta cagactacaa agaccatgac ggtgattata aagatcatga catcgactac
4381 aaggatgacg atgacaagta actgtgcctt ctagttgcca gccatctgtt gtttgcccct
4441 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
4501 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
4561 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
4621 ctatggcacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc
4681 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
4741 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
4801 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
4861 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
4921 ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg
4981 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
5041 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
5101 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
5161 gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt
5221 tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat
5281 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
5341 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
5401 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
5461 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
5521 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
5581 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
5641 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
5701 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
5761 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
5821 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
5881 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
5941 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
6001 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
6061 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
6121 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
6181 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
6241 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
6301 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
6361 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
6421 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
6481 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
6541 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
6601 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
6661 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
6721 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
6781 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
6841 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
6901 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
6961 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
7021 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
7081 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
7141 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
7201 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
7261 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
7321 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
7381 acatgt
//
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pAAVLINK-3'-CIC-FLAG质粒载体应用举例
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