pSTAT3-mScarlet3-iPAK4

LOCUS       pSTAT3-mScarlet3-iPAK4               6473 bp DNA     circular SYN 19-JUL-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 14, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          3570..4646
                     /mol_type="genomic DNA"
                     /organism="."
     polyA_signal    285..406
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(455..471)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    479..495
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(503..533)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    548..569
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    798..1468
                     /label=pUC ori
     rep_origin      complement(857..1442)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1613..2473)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2474..2578)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2497..2515
                     /label=Amp-pro-R
     misc_feature    2641..2741
                     /label=4x STAT binding
     misc_feature    2641..2655
                     /label=STAT3 binding motif
     misc_feature    2665..2684
                     /label=STAT3 binding motif
     misc_feature    2690..2709
                     /label=STAT3 binding motif
     misc_feature    2693..2701
                     /label=TTCNNNgAA
     misc_feature    2715..2734
                     /label=STAT3 binding motif
     misc_feature    2742..2786
                     /label=P_CMVmin
     promoter        2747..2785
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        2830..2848
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2855..2898
                     /label=STAT3-mScarlet3-F
     regulatory      2877..2886
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2883..3569
                     /codon_start=1
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
                     VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     primer_bind     3281..3300
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(3549..3598)
                     /label=iBox-mScarlet3-R
     CDS             3582..3695
                     /codon_start=1
                     /label=INKA-box
                     /translation="EAEDWTAALLNRGRSRQPLVLGDNCFADLVHNWMELPE"
     CDS             3702..4643
                     /codon_start=1
                     /label=Pak4cat
                     /translation="PAAPAVPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLD
                     NFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEM
                     YNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRD
                     IKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIW
                     SLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPA
                     QRATAAELLKHPFLAKAGPPASIVPLMRQNRTR"
     primer_bind     complement(3795..3813)
                     /label=PAK4-R
     primer_bind     complement(4626..4668)
                     /label=HindIII-iPAK4-R
     primer_bind     complement(4676..4693)
                     /label=PcDNA3.1R( BGH )
     polyA_signal    4682..4906
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4786..4805)
                     /label=BGH-New
     rep_origin      4952..5380
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5394..5723
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5574..5709
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(5790..6473,1..111)
                     /label=NeoR
ORIGIN
        1 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
       61 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
      121 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
      181 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
      241 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
      301 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
      361 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
      421 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
      481 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
      541 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
      601 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
      661 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
      721 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
      781 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
      841 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
      901 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
      961 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1021 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1081 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1141 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1201 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1261 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1321 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1381 ccgctggtag cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1441 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     1501 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     1561 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     1621 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     1681 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     1741 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     1801 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     1861 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     1921 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     1981 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     2041 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     2101 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     2161 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     2221 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     2281 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     2341 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     2401 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     2461 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     2521 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     2581 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctccac gcgtctcgag
     2641 atttcccgta aatcgtcgag tggacatttc ccgtaaatcg tcgagtggac atttcccgta
     2701 aatcgtcgag tggacatttc ccgtaaatcg tcgagctgca gtcgagggta ggcgtgtacg
     2761 gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg cttactggct
     2821 tatcgaaatt aatacgactc actataggga gacccaagct gttcgaagcg gaattcgcca
     2881 ccatggatag caccgaggca gtgatcaagg agttcatgcg gttcaaggtg cacatggagg
     2941 gctccatgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     3001 gcacccagac cgccaagctg agggtgacca agggtggccc cctgcccttc tcctgggaca
     3061 tcctgtcccc tcagttcatg tacggctcca gggccttcac gaagcacccc gccgacatcc
     3121 ccgactactg gaagcagtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     3181 aggacggcgg cgccgtgtcc gtggcccagg acacctccct ggaggacggc accctgatct
     3241 acaaggtgaa gctccgcggc accaacttcc ctcctgacgg ccccgtaatg cagaagaaga
     3301 caatgggctg ggaagcatcc accgagcggt tgtaccccga ggacgtcgtg ctgaagggcg
     3361 acattaagat ggccctgcgc ctgaaggacg gcggccgcta cctggcggac ttcaagacca
     3421 cctacagggc caagaagccc gtgcagatgc ccggcgcctt caacatcgac cgcaagttgg
     3481 acatcacatc ccacaacgag gactacaccg tggtggaaca gtacgaacgc tccgtggccc
     3541 gccactccac cggcggctcc ggtggctcct caggtggatc cgaagcagag gactggacag
     3601 cagccctact gaacaggggt cgcagtcgcc agcccctggt actaggggac aattgctttg
     3661 ctgacttggt gcacaactgg atggagctgc ctgaggaatt ccccgccgcc cctgctgttc
     3721 ctgggccccc tggcccccgc tcaccacagc gggagccaca gcgagtatcc catgagcagt
     3781 tccgggctgc cctgcagctg gtggtggacc caggcgaccc ccgctcctac ctggacaact
     3841 tcatcaagat tggcgagggc tccacgggca tcgtgtgcat cgccaccgtg cgcagctcgg
     3901 gcaagctggt ggccgtcaag aagatggacc tgcgcaagca gcagaggcgc gagctgctct
     3961 tcaacgaggt ggtaatcatg agggactacc agcacgagaa tgtggtggag atgtacaaca
     4021 gctacctggt gggggacgag ctctgggtgg tcatggagtt cctggaagga ggcgccctca
     4081 ccgacatcgt cacccacacc aggatgaacg aggagcagat cgcagccgtg tgccttgcag
     4141 tgctgcaggc cctgtcggtg ctccacgccc agggcgtcat ccaccgggac atcaagagcg
     4201 actcgatcct gctgacccat gatggcaggg tgaagctgtc agactttggg ttctgcgccc
     4261 aggtgagcaa ggaagtgccc cgaaggaagt cgctggtcgg cacgccctac tggatggccc
     4321 cagagctcat ctcccgcctt ccctacgggc cagaggtaga catctggtcg ctggggataa
     4381 tggtgattga gatggtggac ggagagcccc cctacttcaa cgagccaccc ctcaaagcca
     4441 tgaagatgat tcgggacaac ctgccacccc gactgaagaa cctgcacaag gtgtcgccat
     4501 ccctgaaggg cttcctggac cgcctgctgg tgcgagaccc tgcccagcgg gccacggcag
     4561 ccgagctgct gaagcaccca ttcctggcca aggcagggcc gcctgccagc atcgtgcccc
     4621 tcatgcgcca gaaccgcacc agatgaaagc tttgaggccg gcctgcaggg atcagcctcg
     4681 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     4741 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     4801 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4861 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4921 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     4981 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     5041 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     5101 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     5161 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     5221 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5281 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5341 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     5401 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5461 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5521 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     5581 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     5641 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     5701 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     5761 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     5821 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     5881 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     5941 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     6001 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     6061 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     6121 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     6181 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     6241 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     6301 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     6361 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     6421 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat att
//