| 质粒名称: | pCMV-Electra1-iPAK4 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000478 |
| 产品用途: | Fluorescent Tickertape Expression |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | Electra1-iPAK4 |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Fluorescent protein-based ticker tapes for multiplexed recordings of transcriptional histories in single cells in culture and in vivo
Wang R., Jiang J., Li Z., Liu T., Wang Y., Xie M., Piatkevich K.D.
Preprint
|
pCMV-Electra1-iPAK4
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pCMV-Electra1-iPAK4载体质粒基本信息
pCMV-Electra1-iPAK4质粒图谱载体图谱和pCMV-Electra1-iPAK4载体序列质粒序列多克隆位点信息
pCMV-Electra1-iPAK4质粒载体简介
pCMV-Electra1-iPAK4质粒序列载体序列
LOCUS pCMV-Electra1-iPAK4 6661 bp DNA circular SYN 09-JUN-2022
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS mVenus N1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6661)
AUTHORS Koushik SV, Chen H, Thaler C, Puhl HL, Vogel SS
TITLE Cerulean, Venus, and VenusY67C FRET reference standards.
JOURNAL Biophys J. 2006 Dec 15. 91(12):L99-L101.
PUBMED 17040988
REFERENCE 2 (bases 1 to 6661)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 13, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Biophys J.
2006 Dec 15. 91(12):L99-L101."
FEATURES Location/Qualifiers
source 1..6661
/mol_type="other DNA"
/organism="synthetic DNA construct"
source 1390..2466
/mol_type="genomic DNA"
/organism="."
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 519..539
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
misc_feature 591..671
/label=MCS
/note="multiple cloning site"
primer_bind 646..684
/label=BamHI-iPAK4-F
primer_bind 651..695
/label=pUBC-Electra-F
regulatory 673..682
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 679..1389
/codon_start=1
/label=Calypso1D6
/translation="MVSKGEELIEENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
RIKVIEGGPLPFAFDILATSFLFGSKTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
VVTVTQDTSLEDGGLVYNVKVRGVNFPSNGPVMQKKTEGWEPFTEMMYPANGGLRGYTD
IALKVDGDGHLHANIVTTYRSKKTVGDIKMPGVHAVDYRLERVEESDNETYVVLREVAV
AKYSNLGGGMDELYK"
primer_bind complement(956..992)
/label=Qch-R
primer_bind 956..992
/label=Qch-F
primer_bind 1369..1415
/label=GFP-iPAK4-F
primer_bind complement(1369..1415)
/label=XFP-iPAK4-R
CDS 1402..1515
/codon_start=1
/label=INKA-box
/translation="EAEDWTAALLNRGRSRQPLVLGDNCFADLVHNWMELPE"
CDS 1522..2463
/codon_start=1
/label=Pak4cat
/translation="PAAPAVPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLD
NFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEM
YNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRD
IKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIW
SLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPA
QRATAAELLKHPFLAKAGPPASIVPLMRQNRTR"
primer_bind complement(1615..1633)
/label=PAK4-R
primer_bind complement(2445..2485)
/label=NotI-PAK4-R
polyA_signal 2588..2709
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2625..2644)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2679..2698
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2716..3171)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2853..2874)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 3065..3084
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 3198..3302
/gene="bla"
/label=AmpR promoter
primer_bind 3286..3330
/label=Primer 1
CDS 3303..4163
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4137..4181)
/label=Primer 3
promoter 4165..4522
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(4188..4208)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 4373..4508
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4435..4454
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(4458..4469)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4557..5351
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4611..4630)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 5221..5240
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(5539..5558)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 5583..5630
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5959..6547
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6448..6467
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
661 gatccaccgg tcgccaccat ggtgtctaag ggcgaagagc tgatcgagga aaatatgcgt
721 atgaaggtgg tcatggaagg ttcggtcaac ggccaccaat tcaaatgcac aggtgaagga
781 gaaggcagac cgtacgaggg agtgcaaacc atgaggatca aagtcatcga gggaggaccc
841 ctgccatttg cctttgacat tcttgccacg tcgttcctgt tcggcagcaa gacctttatc
901 aagtacccgg ccgacatccc tgatttcttc aaacagtcct ttcctgaggg ctttacttgg
961 gaaagagtta cgagatacga agatggtgga gtcgtcaccg tcacgcagga caccagcctt
1021 gaggatgggg ggctcgtcta caacgtcaag gtcagagggg taaactttcc ctccaatggt
1081 cccgtgatgc agaagaagac cgagggttgg gagcctttca cagagatgat gtatccagca
1141 aatggtggtc tgagaggata taccgacatc gcactgaaag ttgatggtga tggccatctg
1201 cacgccaaca tcgtgacaac ttacaggtca aaaaagaccg tcggggacat caagatgccc
1261 ggtgtccatg ccgttgatta ccgcctggaa agggtcgaag agagtgacaa tgaaacctac
1321 gtagtgctaa gagaagtggc agttgccaaa tacagcaacc ttggtggtgg catggacgag
1381 ctgtacaagt caggtggatc cgaagcagag gactggacag cagccctact gaacaggggt
1441 cgcagtcgcc agcccctggt actaggggac aattgctttg ctgacttggt gcacaactgg
1501 atggagctgc ctgaggaatt ccccgccgcc cctgctgttc ctgggccccc tggcccccgc
1561 tcaccacagc gggagccaca gcgagtatcc catgagcagt tccgggctgc cctgcagctg
1621 gtggtggacc caggcgaccc ccgctcctac ctggacaact tcatcaagat tggcgagggc
1681 tccacgggca tcgtgtgcat cgccaccgtg cgcagctcgg gcaagctggt ggccgtcaag
1741 aagatggacc tgcgcaagca gcagaggcgc gagctgctct tcaacgaggt ggtaatcatg
1801 agggactacc agcacgagaa tgtggtggag atgtacaaca gctacctggt gggggacgag
1861 ctctgggtgg tcatggagtt cctggaagga ggcgccctca ccgacatcgt cacccacacc
1921 aggatgaacg aggagcagat cgcagccgtg tgccttgcag tgctgcaggc cctgtcggtg
1981 ctccacgccc agggcgtcat ccaccgggac atcaagagcg actcgatcct gctgacccat
2041 gatggcaggg tgaagctgtc agactttggg ttctgcgccc aggtgagcaa ggaagtgccc
2101 cgaaggaagt cgctggtcgg cacgccctac tggatggccc cagagctcat ctcccgcctt
2161 ccctacgggc cagaggtaga catctggtcg ctggggataa tggtgattga gatggtggac
2221 ggagagcccc cctacttcaa cgagccaccc ctcaaagcca tgaagatgat tcgggacaac
2281 ctgccacccc gactgaagaa cctgcacaag gtgtcgccat ccctgaaggg cttcctggac
2341 cgcctgctgg tgcgagaccc tgcccagcgg gccacggcag ccgagctgct gaagcaccca
2401 ttcctggcca aggcagggcc gcctgccagc atcgtgcccc tcatgcgcca gaaccgcacc
2461 agatgaagcg gccgcgactc tagacataat cagccatacc acatttgtag aggttttact
2521 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
2581 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
2641 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
2701 tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
2761 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
2821 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
2881 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
2941 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
3001 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
3061 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
3121 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca
3181 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
3241 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
3301 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
3361 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
3421 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
3481 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
3541 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
3601 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
3661 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
3721 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
3781 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
3841 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
3901 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
3961 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtggct
4021 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
4081 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
4141 cctcactgat taagcattgg taacctgagg cggaaagaac cagctgtgga atgtgtgtca
4201 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
4261 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
4321 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
4381 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
4441 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
4501 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga
4561 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
4621 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
4681 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
4741 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
4801 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
4861 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
4921 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
4981 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
5041 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
5101 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
5161 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
5221 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
5281 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
5341 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
5401 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
5461 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
5521 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
5581 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg
5641 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
5701 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
5761 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta
5821 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
5881 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
5941 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
6001 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
6061 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
6121 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
6181 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
6241 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
6301 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
6361 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
6421 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
6481 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
6541 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
6601 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca
6661 t
//
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