PCDH-CMV-mCherry-TRIM33

LOCUS       PCDH-CMV-mCherry-TRIM33              10533 bp DNA     circular SYN 04-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10533)
  AUTHORS   15129015045
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10533
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     1904..1953
                     /label=Primer 4
     enhancer        1938..2241
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2242..2445
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2444..2491)
                     /label=Primer 1
     primer_bind     2466..2500
                     /label=Primer 2
     primer_bind     2466..2492
                     /label=Primer 5
     CDS             2496..3203
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     3025..3046
                     /label=mcherry-F
     misc_feature    3204..3232
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     3219..3257
                     /label=TRIM33-F
     primer_bind     3222..3246
                     /label=TRIM28-F
     misc_feature    3240..6623
                     /label=TRIM33
     primer_bind     complement(3673..3691)
                     /label=33-seqR0
     CDS             complement(5056..5067)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6604..6644)
                     /label=TRIM33-R
     misc_feature    6630..7217
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(7101..7112)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7290..7523
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7595..7716
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      7735..7870
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7908..7924)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7932..7948
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7956..7986)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8001..8022
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8310..8898)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9069..9929)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9930..10034)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10403..10416)
                     /label=Primer 5
     primer_bind     10508..10524
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagttgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     1981 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2041 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2101 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2161 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2221 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     2281 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2341 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     2401 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     2461 cagattctag accgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggaggata
     2521 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     2581 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     2641 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     2701 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     2761 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     2821 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     2881 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     2941 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     3001 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     3061 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     3121 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     3181 gcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcggaattca
     3241 tggcggaaaa caaaggcggc ggcgaggctg agagcggcgg cgggggcagc ggcagcgcgc
     3301 cggtaactgc cggggccgcc gggcccgccg cgcaggaggc ggagccgcct ctcaccgcgg
     3361 tgctggtgga ggaggaggag gaggaaggcg gcagggccgg cgctgagggc ggcgcggccg
     3421 ggcccgacga cgggggggtg gccgcggcct cctcgggctc ggcccaggct gcttcatctc
     3481 ctgcggcctc agtgggcact ggagttgccg ggggcgcagt atcgacgccg gctccagctc
     3541 cagcctcggc tcccgctccg ggtccctcgg cagggccgcc tcctggaccg ccagcctcgc
     3601 tcctggacac ctgcgccgtg tgtcagcaga gcttgcagag ccggcgtgag gcggagccca
     3661 agctgctgcc ctgtcttcac tccttctgcc tgcgctgcct gcccgagccg gagcgccagc
     3721 tcagcgtgcc catcccgggg ggcagcaacg gcgacatcca gcaagttggt gtaatacggt
     3781 gcccagtatg ccgccaagaa tgcagacaga tagaccttgt ggataattat tttgtgaaag
     3841 acacatctga agctcctagc agttctgatg aaaaatcaga acaggtatgt actagttgtg
     3901 aagacaatgc aagtgcagtt ggcttttgtg tagaatgtgg agagtggcta tgtaagacat
     3961 gtatcgaagc acatcaaaga gtaaaattta ctaaagatca cttgatcagg aagaaagaag
     4021 atgtctcaga gtctgttgga gcatctggtc aacgccctgt tttctgccct gtacacaaac
     4081 aagaacagtt gaaacttttc tgtgaaacat gtgatagatt gacatgtaga gactgtcagc
     4141 tattggaaca caaagaacat aggtatcagt ttttggaaga agcttttcaa aatcagaagg
     4201 gtgcaattga gaatctactg gcgaaacttc ttgagaagaa gaattatgtt cattttgcag
     4261 ctactcaggt gcagaatagg ataaaagaag taaatgagac taacaaacga gtagaacagg
     4321 aaattaaagt ggccattttc acccttatca atgaaattaa taagaaagga aaatctctct
     4381 tacaacagct agagaatgtt acaaaggaaa gacagatgaa gttactacag cagcagaatg
     4441 acatcacagg cctttcccgg caggtgaagc atgttatgaa cttcacaaat tgggcaattg
     4501 caagtggcag cagcacagca ctactataca gcaagcgact gattactttc cagttgcgtc
     4561 atattttgaa agcacggtgt gatcctgtcc ctgctgctaa tggagcaata cgtttccatt
     4621 gtgatcccac cttctgggca aagaatgtag tcaatttagg taatctagta atagagagta
     4681 aaccagctcc tggttatact cctaatgttg tagttgggca agttcctcca gggacaaacc
     4741 acattagtaa aacccctgga cagattaact tagcacagct tcgactccag cacatgcaac
     4801 aacaagtata tgcacagaaa catcagcagt tgcaacagat gaggatgcag caaccaccag
     4861 cacctgtacc aactacaaca acaacaacac aacagcatcc tagacaagca gcccctcaga
     4921 tgttacaaca acagcctcct cgattgatca gtgtgcaaac aatgcaaaga ggcaacatga
     4981 actgtggagc ttttcaagcc catcagatga gactggctca gaatgctgcc agaataccag
     5041 ggatacccag gcacagcggc cctcaatatt ccatgatgca gccacacctc caaagacaac
     5101 actcaaaccc agggcatgct ggaccctttc ccgtagtatc ggtacacaac accacaatca
     5161 acccaacgag ccctactaca gcaactatgg caaatgcaaa ccgaggtccc accagcccat
     5221 ctgttacagc aatagagcta atcccctcag ttaccaatcc agaaaacctt ccatcgctgc
     5281 cagatattcc acccatacag ttggaagatg ctggctcaag tagtttagat aatctactaa
     5341 gtagatacat ctcaggcagt cacctacccc cacagcctac aagcaccatg aatccttctc
     5401 caggtccctc tgccctttct ccgggatcat caggtttatc caattctcac acacctgtga
     5461 gacccccaag tacttctagt actggcagtc gaggcagctg tgggtcatca ggaagaactg
     5521 ctgagaagac aagtcttagt ttcaaatctg atcaggtgaa ggtcaagcaa gaacctggga
     5581 ctgaagatga aatatgtagc ttttcaggag gtgtaaaaca agaaaaaaca gaggatggca
     5641 ggaggagtgc ttgcatgttg agcagtcctg agagtagctt gacaccacct ctctcaacca
     5701 acctgcatct agaaagtgaa ttggatgcat tggcaagcct ggaaaaccat gtgaaaattg
     5761 aacctgcaga tatgaatgaa agctgcaaac agtcagggct cagcagcctt gttaatggaa
     5821 agtccccaat tcgaagcctc atgcacaggt cggcaaggat tggaggagat ggcaacaata
     5881 aagatgatga cccaaatgaa gactggtgtg ctgtctgcca aaacggagga gatctcttgt
     5941 gctgcgaaaa atgtccaaag gtctttcatc taacttgtca tgttccaaca ctacttagct
     6001 ttccaagtgg ggactggata tgcacatttt gtagagatat tggaaagcca gaagttgaat
     6061 atgattgtga taatttgcaa catagtaaga aggggaaaac tgcgcagggg ttaagccccg
     6121 tggaccaaag gaaatgtgaa cgtcttctgc tttacctcta ttgccatgaa ttaagtattg
     6181 aattccagga gcctgttcct gcttcgatac caaactacta taaaattata aagaaaccaa
     6241 tggatttatc caccgtgaaa aagaagcttc agaaaaaaca ttcccaacac taccaaatcc
     6301 cggatgactt tgtggccgat gtccgtttga tcttcaagaa ctgtgaaagg tttaatgaaa
     6361 tgatgaaagt tgttcaagtt tatgcagaca cacaagagat taatttgaag gctgattcag
     6421 aagtagctca ggcagggaaa gcagttgcat tgtactttga agataaactc acagagatct
     6481 actcagacag gaccttcgca cctttgccag agtttgagca ggaagaggat gatggtgagg
     6541 taactgagga ctctgatgaa gactttatac agccccgcag aaaacgccta aagtcagatg
     6601 agagaccagt acatataaag taaggatcca atcaacctct ggattacaaa atttgtgaaa
     6661 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     6721 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     6781 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     6841 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     6901 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     6961 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     7021 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     7081 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     7141 tgccggctct gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt cggatctccc
     7201 tttggccgcc tccccgccgg tacctttaag accaatgact tacaaggcag ctgtagatct
     7261 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaaata
     7321 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     7381 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7441 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7501 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     7561 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     7621 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7681 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     7741 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7801 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     7861 tttggaggcc tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt
     7921 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     7981 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     8041 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     8101 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     8161 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     8221 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     8281 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     8341 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     8401 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     8461 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     8521 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     8581 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     8641 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     8701 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     8761 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     8821 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     8881 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     8941 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     9001 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     9061 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     9121 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     9181 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     9241 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     9301 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     9361 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     9421 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     9481 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     9541 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     9601 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     9661 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     9721 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     9781 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     9841 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     9901 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     9961 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    10021 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
    10081 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
    10141 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
    10201 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
    10261 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
    10321 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc
    10381 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
    10441 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
    10501 cgacgttgta aaacgacggc cagtgccaag ctg
//