| 质粒名称: | pLZ340 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000615 |
| 产品用途: | Mammalian Cre-dependent GEMSANR expression vector (PhEF1α-loxP-STOP-loxP-GEMSANR-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: |
EF1a
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | GEMSANR |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Multi-layered computational gene networks by engineered tristate logics
Shao J., Qiu X., Zhang L., Li S., Xue S., Si Y., Li Y., Jiang J., Wu Y., Xiong Q., Wang Y., Chen Q., Gao T., Zhu L., Wang H., Xie M.
Cell 187, 5064-5080.e14 (2024)
|
pLZ340
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pLZ340载体质粒基本信息
pLZ340质粒图谱载体图谱和pLZ340载体序列质粒序列多克隆位点信息
pLZ340质粒载体简介
pLZ340质粒序列载体序列
LOCUS pLZ340 9231 bp DNA circular SYN 13-JAN-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9231)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Jul 19, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9231
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232
/label=CMV
misc_feature 234..1300
/label=P_EF1a
intron 353..1291
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
promoter 1309..1313
/label=P_TAL
misc_recomb 1329..1362
/label=LoxP
protein_bind complement(1329..1362)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
primer_bind 1370..1396
/label=192-WKG-615-F
polyA_signal 1418..1642
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
terminator 1651..1835
/label=SV40 late polyA
polyA_signal 1653..1774
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
misc_feature 1872..1905
/label=loxP
protein_bind complement(1872..1905)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
polyA_signal 1912..1960
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
regulatory 1980..1989
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
misc_feature 1986..2048
/label=Igk secretion signal
CDS 1986
/codon_start=1
/product="start codon"
/label=ATG
/translation=""
CDS 1989
/codon_start=1
/label=mCherry
/translation=""
regulatory 2061..2070
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 2069
/codon_start=1
/label=5xGS Linker
/translation=""
CDS 2070..2132
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 2133..2207
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 2208..2270
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 2271..2345
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 2352..2414
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 2415..2489
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 2490..2552
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 2553..2627
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 2634..2696
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 2697..2771
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 2772..2834
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 2835..2909
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 2916..2978
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 2979..3053
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 3054..3116
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 3117..3191
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
misc_feature 3204..3947
/label=mouse EpoR-D1_D2_TM
/note="mouse EpoR-D1_D2_TM"
primer_bind 3207..3228
/label=193-WKG-615-F
primer_bind complement(3271..3293)
/label=186-WKG-603-R
misc_feature 3477..3479
/label=F93A
/note="F93A"
misc_feature 3931..3947
/label=EpoR-D1_D2_TM
misc_feature 3957..4785
/label=IL6ST
misc_feature 3984..4010
/label=box1
misc_feature 4308..4310
/label=Y759A
polyA_signal 4828..5052
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 5098..5526
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5112..5136)
/label=184-WKG-599-R
primer_bind 5397..5416
/label=189-WKG-612-F
promoter 5540..5869
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
misc_feature 5720..5797
/label=SV40 ORI
CDS 5936..6730
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 6904..7025
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7070..7090)
/label=M13-rev
primer_bind complement(7074..7090)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
misc_binding complement(7096..7118)
/label=LacO
protein_bind 7098..7114
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7122..7152)
/label=lac promoter
/note="promoter for the E. coli lac operon"
promoter complement(7123..7152)
/label=lac
primer_bind complement(7135..7158)
/label=190-WKG-612-R
protein_bind 7167..7188
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
misc_feature 7294..8234
/label=ColE1
/note="ColE1"
CDS complement(8235..9095)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(9096..9200)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtgggaaag
241 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
301 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
361 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
421 tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
481 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
541 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
601 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
661 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
721 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
781 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
841 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
901 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
961 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
1021 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
1081 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
1141 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
1201 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
1261 agtggttcaa agtttttttc ttccatttca ggtgtcgtga ggccggccga agcagaaggc
1321 tagcgcttat aacttcgtat agcatacatt atacgaagtt atcggcgcgc caatgctccg
1381 aatgcatcag tgcccctgca gggctgatca gcctcgactg tgccttctag ttgccagcca
1441 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
1501 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
1561 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
1621 ggggatgcgg tgggctctat ggcttctgag gataagatac attgatgagt ttggacaaac
1681 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
1741 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
1801 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc gcttggtacc gtctttccag
1861 tggttaatta aataacttcg tatagcatac attatacgaa gttatcgatc cgaacaaacg
1921 acccaacacc cgtgcgtttt attctgtctt tttattgccg atcctcgagg aattcgccgg
1981 ccaccatgga gaccgatacc ttattactgt gggtgcttct tctgtgggtt cccggtagca
2041 ccggagacgg atccaagctt gccaccatgg gggagctcga cgagttggtt tatctcttag
2101 acggacccgg atatgacccc attcattcag atggctccgg aacgggcagc gggaccggaa
2161 gtggcaccgg aaccacttcc ggaaccggta ctggcgggag taccggcggt gagctagacg
2221 aactcgtgta cctgttggat ggccccggtt acgacccaat ccacagcgat ggaagtggaa
2281 ctgggtctgg gactgggtcc ggcaccggga ctacttcagg caccggcact gggggctcca
2341 ctggccatac cggggagctc gacgagttgg tttatctctt agacggaccc ggatatgacc
2401 ccattcattc agatggctcc ggaacgggca gcgggaccgg aagtggcacc ggaaccactt
2461 ccggaaccgg tactggcggg agtaccggcg gtgagctaga cgaactcgtg tacctgttgg
2521 atggccccgg ttacgaccca atccacagcg atggaagtgg aactgggtct gggactgggt
2581 ccggcaccgg gactacttca ggcaccggca ctgggggctc cactggccat accggggagc
2641 tcgacgagtt ggtttatctc ttagacggac ccggatatga ccccattcat tcagatggct
2701 ccggaacggg cagcgggacc ggaagtggca ccggaaccac ttccggaacc ggtactggcg
2761 ggagtaccgg cggtgagcta gacgaactcg tgtacctgtt ggatggcccc ggttacgacc
2821 caatccacag cgatggaagt ggaactgggt ctgggactgg gtccggcacc gggactactt
2881 caggcaccgg cactgggggc tccactggcc ataccgggga gctcgacgag ttggtttatc
2941 tcttagacgg acccggatat gaccccattc attcagatgg ctccggaacg ggcagcggga
3001 ccggaagtgg caccggaacc acttccggaa ccggtactgg cgggagtacc ggcggtgagc
3061 tagacgaact cgtgtacctg ttggatggcc ccggttacga cccaatccac agcgatggaa
3121 gtggaactgg gtctgggact gggtccggca ccgggactac ttcaggcacc ggcactgggg
3181 gctccactgg ccatatggaa ttcgcacctt cacccagcct cccggacccc aagtttgaga
3241 gcaaagcggc cctgctggca tcccggggct ccgaagaact tctgtgcttc acccaacgct
3301 tggaagactt ggtgtgtttc tgggaggaag cggcgagctc cgggatggac ttcaactaca
3361 gcttctcata ccagctcgag ggtgagtcac gaaagtcatg tagcctgcac caggctccca
3421 ccgtccgcgg ctccgtgcgt ttctggtgtt cactgccaac agcggacaca tcgagtgctg
3481 tgccgctgga gctgcaggtg acggaggcgt ccggttctcc tcgctatcac cgcatcatcc
3541 atatcaatga agtagtgctc ctggacgccc ccgcggggct gctggcgcgc cgggcagaag
3601 agggcagcca cgtggtgctg cgctggctgc cacctcctgg agcacctatg accacccaca
3661 tccgatatga agtggacgtg tcggcaggca accgggcagg agggacacaa agggtggagg
3721 tcctggaagg ccgcactgag tgtgttctga gcaacctgcg gggcgggacg cgctacacct
3781 tcgctgttcg agcgcgcatg gccgagccga gcttcagcgg attctggagt gcctggtctg
3841 agcccgcgtc actactgacc gctagcgacc tggaccctct catcttgacg ctgtctctca
3901 ttctggtcct catctcgttg ttgctgacgg ttctggccct gctgtccgca gcagcaaata
3961 agcgagacct aattaaaaaa cacatctggc ctaatgttcc agatccttca aagagtcata
4021 ttgcccagtg gtcacctcac actcctccaa ggcacaattt taattcaaaa gatcaaatgt
4081 attcagatgg caatttcact gatgtaagtg ttgtggaaat agaagcaaat gacaaaaagc
4141 cttttccaga agatctgaaa tcattggacc tgttcaaaaa ggaaaaaatt aatactgaag
4201 gacacagcag tggtattggg gggtcttcat gcatgtcatc ttctaggcca agcatttcta
4261 gcagtgatga aaatgaatct tcacaaaaca cttcgagcac tgtccaggct tctaccgtgg
4321 tacacagtgg ctacagacac caagttccgt cagtccaagt cttctcaaga tccgagtcta
4381 cccagccctt gttagattca gaggagcggc cagaagatct acaattagta gatcatgtag
4441 atggcggtga tggtattttt cccaggcaac agtacttcaa acagaactgc agtcagcatg
4501 aatccagtcc agatatttca cattttgaaa ggtcaaagca agtttcatca gtcaatgagg
4561 aagattttgt tagacttaaa cagcagattt cagatcatat ttcacaatcc tgtggatctg
4621 ggcaaatgaa aatgtttcag gaagtttctg cagcagatgc ttttggtcca ggtactgagg
4681 gacaagtaga aagatttgaa acagttggca tggaggctgc gactgatgaa ggcatgccta
4741 aaagttactt accacagact gtacggcaag gcggctacat gcctcagtaa tctagagggc
4801 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
4861 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
4921 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
4981 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
5041 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
5101 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
5161 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
5221 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
5281 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
5341 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
5401 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
5461 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
5521 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
5581 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
5641 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
5701 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
5761 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
5821 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
5881 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
5941 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
6001 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
6061 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
6121 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
6181 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
6241 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
6301 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
6361 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
6421 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
6481 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
6541 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
6601 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
6661 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
6721 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
6781 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
6841 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
6901 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
6961 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
7021 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
7081 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
7141 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
7201 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
7261 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
7321 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
7381 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
7441 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
7501 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
7561 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
7621 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
7681 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
7741 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
7801 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
7861 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
7921 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
7981 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
8041 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
8101 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
8161 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
8221 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
8281 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
8341 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
8401 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
8461 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
8521 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
8581 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
8641 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
8701 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
8761 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
8821 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
8881 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
8941 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
9001 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
9061 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
9121 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
9181 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
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