pCVB

LOCUS       pCVB               15775 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCVB
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15775)
  AUTHORS   Tang Y, Wang F, Zhao J, Xie K, Hong Y, Liu Y
  TITLE     Virus-based microRNA expression for gene functional analysis in 
            plants.
  JOURNAL   Plant Physiol. 2010 Jun;153(2):632-41. Epub 2010 Apr 13.
  PUBMED    20388670
REFERENCE   2  (bases 1 to 15775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15775
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     intron          10..199
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             2024..2041
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2076..2328
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2350..2374
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3674..4303
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4737..5804
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      5870..6064
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6300..6322)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6408..6548
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6463..6482
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6563..6580)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6734..7322)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6814..6833)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7409..8203)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8628..8652
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    8730..8904
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8944..9969)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(10037..10714)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    10905..10926
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10941..10971
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10979..10995
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10984..11006
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11003..11019
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11003..11019
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(15015..15032)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(15015..15031)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15024..15046)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        15408..15753
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     15696..15716
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             join(15770..9,200..2017)
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELEVTAKSQTESDIYPLRVGIRS
                     VAVKGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
ORIGIN
        1 gatctgaggg taaatttcta gtttttctcc ttcattttct tggttaggac ccttttctct
       61 ttttattttt ttgagctttg atctttcttt aaactgatct attttttaat tgattggtta
      121 tggtgtaaat attacatagc tttaactgat aatctgatta ctttatttcg tgtgtctatg
      181 atgatgatga tagttacaga accgacgact cgtccgtcct gtagaaaccc caacccgtga
      241 aatcaaaaaa ctcgacggcc tgtgggcatt cagtctggat cgcgaaaact gtggaattga
      301 tcagcgttgg tgggaaagcg cgttacaaga aagccgggca attgctgtgc caggcagttt
      361 taacgatcag ttcgccgatg cagatattcg taattatgcg ggcaacgtct ggtatcagcg
      421 cgaagtcttt ataccgaaag gttgggcagg ccagcgtatc gtgctgcgtt tcgatgcggt
      481 cactcattac ggcaaagtgt gggtcaataa tcaggaagtg atggagcatc agggcggcta
      541 tacgccattt gaagccgatg tcacgccgta tgttattgcc gggaaaagtg tacgtatcac
      601 cgtttgtgtg aacaacgaac tgaactggca gactatcccg ccgggaatgg tgattaccga
      661 cgaaaacggc aagaaaaagc agtcttactt ccatgatttc tttaactatg ccggaatcca
      721 tcgcagcgta atgctctaca ccacgccgaa cacctgggtg gacgatatca ccgtggtgac
      781 gcatgtcgcg caagactgta accacgcgtc tgttgactgg caggtggtgg ccaatggtga
      841 tgtcagcgtt gaactgcgtg atgcggatca acaggtggtt gcaactggac aaggcactag
      901 cgggactttg caagtggtga atccgcacct ctggcaaccg ggtgaaggtt atctctatga
      961 actcgaagtc acagccaaaa gccagacaga gtctgatatc tacccgcttc gcgtcggcat
     1021 ccggtcagtg gcagtgaagg gccaacagtt cctgattaac cacaaaccgt tctactttac
     1081 tggctttggt cgtcatgaag atgcggactt acgtggcaaa ggattcgata acgtgctgat
     1141 ggtgcacgac cacgcattaa tggactggat tggggccaac tcctaccgta cctcgcatta
     1201 cccttacgct gaagagatgc tcgactgggc agatgaacat ggcatcgtgg tgattgatga
     1261 aactgctgct gtcggctttc agctgtcttt aggcattggt ttcgaagcgg gcaacaagcc
     1321 gaaagaactg tacagcgaag aggcagtcaa cggggaaact cagcaagcgc acttacaggc
     1381 gattaaagag ctgatagcgc gtgacaaaaa ccacccaagc gtggtgatgt ggagtattgc
     1441 caacgaaccg gatacccgtc cgcaaggtgc acgggaatat ttcgcgccac tggcggaagc
     1501 aacgcgtaaa ctcgacccga cgcgtccgat cacctgcgtc aatgtaatgt tctgcgacgc
     1561 tcacaccgat accatcagcg atctctttga tgtgctgtgc ctgaaccgtt attacggatg
     1621 gtatgtccaa agcggcgatt tggaaacggc agagaaggta ctggaaaaag aacttctggc
     1681 ctggcaggag aaactgcatc agccgattat catcaccgaa tacggcgtgg atacgttagc
     1741 cgggctgcac tcaatgtaca ccgacatgtg gagtgaagag tatcagtgtg catggctgga
     1801 tatgtatcac cgcgtctttg atcgcgtcag cgccgtcgtc ggtgaacagg tatggaattt
     1861 cgccgatttt gcgacctcgc aaggcatatt gcgcgttggc ggtaacaaga aagggatctt
     1921 cactcgcgac cgcaaaccga agtcggcggc ttttctgctg caaaaacgct ggactggcat
     1981 gaacttcggt gaaaaaccgc agcagggagg caaacaagct agccaccacc accaccacca
     2041 cgtgtgaatt acaggtgacc agctcgaatt tccccgatcg ttcaaacatt tggcaataaa
     2101 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
     2161 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
     2221 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
     2281 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattaaact
     2341 atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa
     2401 taacggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc
     2461 caaccacagg gttcccctcg ggatcaaagt actttgatcc aacccctccg ctgctatagt
     2521 gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc
     2581 taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt
     2641 agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat gaacaagagc
     2701 gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga cttgaccaac
     2761 caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa gatcaccggc
     2821 accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc tggcgacgtt
     2881 gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact ggacattgcc
     2941 gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc
     3001 accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt
     3061 tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag
     3121 tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga gctgatcgac
     3181 caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg
     3241 taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc
     3301 ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa
     3361 gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc attaccgaag
     3421 agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa
     3481 ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg gcctggccgg
     3541 ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt
     3601 aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac
     3661 gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac
     3721 gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt
     3781 cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct
     3841 aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg
     3901 cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa
     3961 ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc
     4021 cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc
     4081 ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct
     4141 ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg
     4201 cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga
     4261 ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag
     4321 aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag
     4381 gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg
     4441 ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc
     4501 gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag
     4561 tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga
     4621 cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt
     4681 tgtctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgacggtc
     4741 gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt
     4801 tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat
     4861 cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg
     4921 cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc
     4981 tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt
     5041 cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag
     5101 aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg
     5161 cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg
     5221 gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg
     5281 gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca
     5341 tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct
     5401 tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg
     5461 agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg
     5521 ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac
     5581 gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg
     5641 gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg
     5701 acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc
     5761 gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc
     5821 tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tctctttcct gtggatagca
     5881 cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa
     5941 agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg
     6001 atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg
     6061 cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc
     6121 tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa
     6181 tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg
     6241 accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     6301 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     6361 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     6421 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     6481 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     6541 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6601 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     6661 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6721 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6781 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6841 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6901 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6961 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7021 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7081 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7141 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     7201 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7261 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7321 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7381 aagggatttt ggtcatgcat tctaggtact aaaacaattc atccagtaaa atataatatt
     7441 ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt
     7501 cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg
     7561 ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt
     7621 tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta
     7681 aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat
     7741 ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc
     7801 tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat
     7861 acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc
     7921 catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct
     7981 ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat
     8041 aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca
     8101 ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt
     8161 cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc
     8221 ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga actccaattc
     8281 actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc
     8341 aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt gatcacaggc
     8401 agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc
     8461 aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc
     8521 cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt
     8581 ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat
     8641 tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg
     8701 tactgaatta acgccgaatt aattcggggg atctggattt tagtactgga ttttggtttt
     8761 aggaattaga aattttattg atagaagtat tttacaaata caaatacata ctaagggttt
     8821 cttatatgct caacacatga gcgaaaccct ataggaaccc taattccctt atctgggaac
     8881 tactcacaca ttattatgga gaaactcgag cttgtcgatc gacagatccg gtcggcatct
     8941 actctatttc tttgccctcg gacgagtgct ggggcgtcgg tttccactat cggcgagtac
     9001 ttctacacag ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg tacgcccgac
     9061 agtcccggct ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc
     9121 gaaattgccg tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga gcatatacgc
     9181 ccggagtcgt ggcgatcctg caagctccgg atgcctccgc tcgaagtagc gcgtctgctg
     9241 ctccatacaa gccaaccacg gcctccagaa gaagatgttg gcgacctcgt attgggaatc
     9301 cccgaacatc gcctcgctcc agtcaatgac cgctgttatg cggccattgt ccgtcaggac
     9361 attgttggag ccgaaatccg cgtgcacgag gtgccggact tcggggcagt cctcggccca
     9421 aagcatcagc tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt
     9481 ttgccagtga tacacatggg gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta
     9541 ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa gatcggccgc
     9601 agcgatcgca tccatagcct ccgcgaccgg ttgtagaaca gcgggcagtt cggtttcagg
     9661 caggtcttgc aacgtgacac cctgtgcacg gcgggagatg caataggtca ggctctcgct
     9721 aaactcccca atgtcaagca cttccggaat cgggagcgcg gccgatgcaa agtgccgata
     9781 aacataacga tctttgtaga aaccatcggc gcagctattt acccgcagga catatccacg
     9841 ccctcctaca tcgaagctga aagcacgaga ttcttcgccc tccgagagct gcatcaggtc
     9901 ggagacgctg tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg
     9961 ctttttcata tctcattgcc ccccgggatc tgcgaaagct cgagagagat agatttgtag
    10021 agagagactg gtgatttcag cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg
    10081 aaggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca
    10141 tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt
    10201 gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc
    10261 tttatcgcaa tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa
    10321 gtgacagata gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa
    10381 agtctcaata gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag
    10441 agtgtcgtgc tccaccatgt tatcacatca atccacttgc tttgaagacg tggttggaac
    10501 gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc
    10561 agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc
    10621 ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag
    10681 gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt
    10741 atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttgg caagctgctc
    10801 tagccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
    10861 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
    10921 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
    10981 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacga attcgagctc
    11041 ggtacccggg gatcccccat cgtggtagat ccttcaagat ccaccatcaa agtgtctttc
    11101 tccacagaca atacgcgttt caaaacatgg aggacgtaat aacgatcttt caaaccgggt
    11161 gtaatagcta agttcccatg gctatggatt cttgcaccaa atatttcatc aaatgtatgc
    11221 aaacctccag tggagcttaa atgtggtttg cgatcaacaa caataacaag agagaacaca
    11281 ccctctatct tcgaaattac tccgtccatg ttcacatcag catgtacacg ctcaatctta
    11341 acggttccct taaatgataa ccggttcaac ttaatatacg acctgcttcg gttaggttca
    11401 atcttaccaa gtacagggaa attaataaac gttgaaaggg ctgagttatg ggtcatgaca
    11461 aattcaggcc catattgatt ttcatgtatg cgttgcaact tcattttagg ctcatcatgg
    11521 gctttacttg atgggccttt acgacgtttc ccatcagtgc gtctaacaaa agttccacgc
    11581 ttagacgatt gattacgtga aacaaatcgt ctttgagagt aagatacccc acgcctaaac
    11641 tttgtaggat acattattct cagcacctct acaaaatgat ttgttatttt aatgttattg
    11701 tcaaagacga tttaaaatat taaactatcg tccattatat agacaaggca aatacaaata
    11761 agcgatagcc taactccgta gaggaaatat aattgggcca cgttcaatat attgtacaat
    11821 gtgtcagaag ctttgtcgtt acaaagaccg gtcaagatag acatatacgc ccaattattt
    11881 cgaacttcaa attaaaggtt taacaccgag ataagcgtta gccaacgctc ctcgacgcgc
    11941 cacgtcgaga gatgagagag agtgcgccaa aggggagcgg gagatggtcc ccagcaccag
    12001 gactccaaaa atttgcggcc atccggtaat attattacgg atggccgctg agatgtgtac
    12061 acgtgtccta tggagtcccc agaggctttt agtatttata ggacaccaag acaccagaaa
    12121 caataggttt tagacgagag ctctcctggt gtccatgaac atatttacga atatgccact
    12181 aaacataaca ttcgaagctt ttttaatttc ttcaaatttg ctctctatat aatcagactt
    12241 agtcttagat tattatactc atgttatctg aataacctaa catactcatc cttcgttaga
    12301 actatcgtta ttccctttct gtttagaaac ccagattttc tctcttcctc ctttcgaaac
    12361 aggtcgtttc atttttctgt tcttcaacac agtttttact aaacccagat attattgaag
    12421 cccctgccgc tgcgcggtcg gatttctata aatgaattct cagttagcga atgctccaaa
    12481 tgcgtttaat tacatagagt ctcatagaga cgaatatcag ctgtcgcatg atcttactga
    12541 gatactactt caattccctt caactgcagc gcaatttaca gcaaggctta atcgtagctg
    12601 tatgaaaatc gaccattgcg tcatcgaata tagacagcaa gtaccaatta acgcaactgg
    12661 atctgtgata gtggagatcc atgacaagag gatgactgac gacgaatcct tacaagcgtc
    12721 ttggacattt cccttaagat gcaacataga tctccactat ttttcatctt cattcttctc
    12781 tctaaaagac cccattccgt ggaaactcta ttacagagtt agcgacacaa atgtgcatca
    12841 acgaacacat tttgccaagt ttaaggggaa attgaaattg tccacagcta aacattcagt
    12901 ggatatcccc ttccgggcac caacagtgaa aattcattca aaacaatttt cacatagaga
    12961 tgttgatttc tcacatgtgg actacggaag atgggagagg aagaccttga ggtccaaatc
    13021 attgtcaaga attggactaa caggcccaat tgaattacaa ccaggtgatt catgggcttc
    13081 aaggagcaca attgggtttc ccaacccaca cacagaatca gaagtggaaa acgcgctaca
    13141 tccatataga gaactcaacc tgctaggaac tagcgcatta gaccccgggg attcagcatc
    13201 acaagctggg ttacagaggg cccaatcaac cataacgatg tcagtggccc aattaagcga
    13261 gctagttagg acgacggttc aggaatgtat taacaataat tgtaatcctc cacaaccaaa
    13321 gtcattgcaa taaaattgcg tgttttatta ttaatgcata tattcaatct taacgccatt
    13381 ttattcatgg ttaacctaaa taatcaagat cgtaagatac aaaggtcgat gccttagaca
    13441 tagcatcgga catccaacaa taatatacca aaatggcgtt tttgcttata ttcgcataaa
    13501 caccgttaca taagtcatgt tcaagatcgt taaatgaggc ccaacagtta taacgcctat
    13561 tagatatcgt ggtagatcct tcaagatcca ccatcaaagt gtctttctcc acagacaata
    13621 cgcgtttcaa aacatggagg acgtaataac gatctttcaa accgggtgta atagctaagt
    13681 tcccatggct atggattctt gcaccaaata tttcatcaaa tgtatgcaaa cctccagtgg
    13741 agcttaaatg tggtttgcga tcaacaacaa taacaagaga gaacacaccc tctatcttcg
    13801 aaattactcc gtccatgttc acatcagcat gtacacgctc aatcttaacg gttcccttaa
    13861 atgataaccg gttcaactta atatacgacc tgcttcggtt aggttcaatc ttaccaagta
    13921 cagggaaatt aataaacgtt gaaagggctg agttatgggt catgacaaat tcaggcccat
    13981 attgattttc atgtatgcgt tgcaacttca ttttaggctc atcatgggct ttacttgatg
    14041 ggcctttacg acgtttccca tcagtgcgtc taacaaaagt tccacgctta gacgattgat
    14101 tacgtgaaac aaatcgtctt tgagagtaag ataccccacg cctaaacttt gtaggataca
    14161 ttattctcag cacctctaca aaatgatttg ttattttaat gttattgtca aagacgattt
    14221 aaaatattaa actatcgtcc attatataga caaggcaaat acaaataagc gatagcctaa
    14281 ctccgtagag gaaatataat tgggccacgt tcaatatatt gtacaatgtg tcagaagctt
    14341 tgtcgttaca aagaccggtc aagatagaca tatacgccca attatttcga acttcaaatt
    14401 aaaggtttaa caccgagata agcgttagcc aacgctcctc gacgcgccac gtcgagagat
    14461 gagagagagt gcgccaaagg ggagcgggag atggtcccca gcaccaggac tccaaaaatt
    14521 tgcggccatc cggtaatatt attacggatg gccgctgaga tgtgtacacg tgtcctatgg
    14581 agtccccaga ggcttttagt atttatagga caccaagaca ccagaaacaa taggttttag
    14641 acgagagctc tcctggtgtc catgaacata tttacgaata tgccactaaa cataacattc
    14701 gaagcttttt taatttcttc aaatttgctc tctatataat cagacttagt cttagattat
    14761 tatactcatg ttatctgaat aacctaacat actcatcctt cgttagaact atcgttattc
    14821 cctttctgtt tagaaaccca gattttctct cttcctcctt tcgaaacagg tcgtttcatt
    14881 tttctgttct tcaacacagt ttttactaaa cccagatatt attgaagccc ctgccgctgc
    14941 gcggtcggat ttctataaat gaattcgata tcaagcttat cgataccgtc gacctgcagg
    15001 catgcaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
    15061 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
    15121 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ctagagcagc
    15181 ttgagcttgg atcagattgt cgtttcccgc cttcagttta gcttcatgga gtcaaagatt
    15241 caaatagagg acctaacaga actcgccgta aagactggcg aacagttcat acagagtctc
    15301 ttacgactca atgacaagaa gaaaatcttc gtcaacatgg tggagcacga cacacttgtc
    15361 tactccaaaa atatcaaaga tacagtctca gaagaccaaa gggcaattga gacttttcaa
    15421 caaagggtaa tatccggaaa cctcctcgga ttccattgcc cagctatctg tcactttatt
    15481 gtgaagatag tggaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag
    15541 gccatcgttg aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg
    15601 agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat
    15661 atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct
    15721 atataaggaa gttcatttca tttggagaga acacggggga ctcttgacca tggta
//