pUBC-SYT1_I368T-mBaoJin

LOCUS       pUBC-SYT1_I368T-mBaoJin               6459 bp DNA     circular SYN 26-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pUBC-SYT1_I368T-mBaoJin
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6459)
  AUTHORS   Ruizhao Wang
  TITLE     Direct Submission
  JOURNAL   Exported Aug 26, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      55..60
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             61..1326
                     /codon_start=1
                     /label=SYT1/I368T
                     /translation="MVSESHHEALAAPPVTTVATVLPSNATEPASPGEGKEDAFSKLKE
                     KFMNELHKIPLPPWALIAIAIVAVLLVLTCCFCICKKCLFKKKNKKKGKEKGGKNAINM
                     KDVKDLGKTMKDQALKDDDAETGLTDGEEKEEPKEEEKLGKLQYSLDYDFQNNQLLVGI
                     IQAAELPALDMGGTSDPYVKVFLLPDKKKKFETKVHRKTLNPVFNEQFTFKVPYSELGG
                     KTLVMAVYDFDRFSKHDIIGEFKVPMNTVDFGHVTEEWRDLQSAEKEEQEKLGDICFSL
                     RYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTIKKNTLNPYY
                     NESFSFEVPFEQIQKVQVVVTVLDYDKTGKNDAIGKVFVGYNSTGAELRHWSDMLANPR
                     RPIAQWHTLQVEEEVDAMLAVKK"
     misc_feature    1162..1164
                     /label=Mutation
     transit_peptide 1327..1401
                     /codon_start=1
                     /label=Linker25
                     /translation="GGSGGTGGSGGTGGSGGTGGSGGTG"
     CDS             1402..1428
                     /codon_start=1
                     /label=HA tag
                     /translation="YPYDVPDYA"
     primer_bind     1431..1453
                     /label=0709-EB3-fmStayG-F
     CDS             1432..2133
                     /codon_start=1
                     /label=mBaoJin
                     /translation="MVSKGEEENMASTPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKG
                     KYVCTSGKLPMSWAALGTTFGYGMKYYTKYPSGLKNWFREVMPGGFTYDRHIQYKGDGS
                     IHAKHQHFMKNGTYHNIVEFTGQDFKENSPVLTGDMNVSLPNEVPQIPRDDGVECPVTL
                     LYPLLSDKSKYVEAHQYTICKPLHNQPAPDVPYHWIRKQYTQSKDDAEERDHICQSETL
                     EAHLKGMDELYK"
     primer_bind     complement(2112..2134)
                     /label=0709-EB3-fmStayG-R
     polyA_signal    2288..2343
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2704..2720)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2728..2744
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2752..2782)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     2757..2778
                     /label=lac-pro-F
     protein_bind    2797..2818
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2877..3072
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        2902..3072
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2923..3058
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3078..3212
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3451..4039)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3455..3474)
                     /label=pUBC-primer-1
     CDS             complement(4210..5070)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4241..4262)
                     /label=Amp-F
     primer_bind     4984..5004
                     /label=Amp-R-seq
     promoter        complement(5071..5174)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5247..5646
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(5374..5396)
                     /label=hUBC-pro-R
     primer_bind     5842..5865
                     /label=pUBC-seq-2
     promoter        5933..6459
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6296..6315
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 gaagcttggg ctgcaggtcg agctcaagct tcgaattctg cagtcgacgg taccgccacc
       61 atggtgagcg agagtcacca tgaggccctg gcagccccgc ctgtcaccac tgtcgcgact
      121 gttctgccaa gcaatgccac agagccagcc agtcctggag aaggaaagga agatgcattt
      181 tctaagctga aggagaagtt tatgaatgag ttgcataaaa ttccattgcc accgtgggcc
      241 ttaattgcaa tagccatagt cgcagtcctt ttagtcctga cctgctgctt ttgtatctgt
      301 aagaaatgtt tgttcaaaaa gaaaaacaag aagaagggaa aggaaaaagg agggaagaat
      361 gccattaaca tgaaagatgt aaaagactta gggaagacga tgaaagatca ggccctcaag
      421 gatgatgatg ctgaaactgg attgacagat ggagaagaaa aagaagaacc caaagaagag
      481 gagaaactgg gaaaacttca gtattcactg gattatgatt tccaaaataa ccagctgctg
      541 gtagggatca ttcaggctgc cgaactgccc gccttggaca tggggggcac atctgatcct
      601 tacgtgaaag tgtttctgct acctgataag aagaagaaat ttgagacaaa agtccaccga
      661 aaaaccctta atcctgtctt caatgagcaa tttactttca aggtaccata ctcggaattg
      721 ggtggcaaaa ccctagtgat ggctgtatat gattttgatc gtttctctaa gcatgacatc
      781 attggagaat ttaaagtccc tatgaacaca gtggattttg gccatgtaac tgaggaatgg
      841 cgtgacctgc aaagtgctga gaaggaagag caagagaaat tgggtgatat ctgcttctcc
      901 cttcgctacg tacctactgc tggtaagctg actgttgtca ttctggaggc aaagaacctg
      961 aagaagatgg atgtgggtgg cttatccgat ccttatgtga agattcatct gatgcagaat
     1021 ggtaagaggc tgaagaagaa aaagacaaca attaaaaaga acacacttaa cccctactac
     1081 aatgagtcat tcagctttga agtacctttt gaacaaatcc agaaagtgca ggtggtggta
     1141 actgttttgg actatgacaa gactggcaag aacgatgcca tcggcaaagt ctttgtgggc
     1201 tacaacagca ccggcgcgga gctgcgacac tggtcagaca tgctggccaa ccccaggcga
     1261 cctattgccc agtggcacac cctgcaggta gaggaggaag ttgatgccat gctggccgtc
     1321 aagaagggcg gaagcggcgg gaccggcggc agcggaggga ccggggggag cggcggcacc
     1381 ggaggaagcg ggggaaccgg ttatccatac gacgtgcctg attatgcagg catggtctca
     1441 aagggagagg aggaaaacat ggctagtaca ccatttaaat ttcaacttaa aggaaccatc
     1501 aatggcaaat cgtttaccgt tgaaggcgaa ggtgaaggga actcacatga aggttctcat
     1561 aaaggaaaat atgtttgtac aagtggaaaa ctaccgatgt catgggcagc acttgggaca
     1621 acctttggtt atggaatgaa atattatacc aaatatccta gtggactgaa gaactggttt
     1681 cgtgaagtaa tgcccggagg ctttacctac gatcgtcata ttcaatataa aggcgatggg
     1741 agtatccatg caaaacacca acactttatg aaaaatggga cttatcacaa cattgtagaa
     1801 tttaccggtc aggattttaa agaaaatagt ccagtcttaa ctggagatat gaatgtctca
     1861 ttaccgaatg aagtcccaca aatacccaga gatgatggag tagaatgccc agtgaccttg
     1921 ctttatcctt tattatcgga taaatcaaaa tacgtcgagg ctcaccaata tacaatctgc
     1981 aagcctcttc ataatcaacc agcacctgat gtcccatatc actggattcg taaacaatac
     2041 acacaaagca aagatgatgc cgaggaacgc gatcatatct gtcaatcaga gactctcgaa
     2101 gcacacttaa agggcatgga cgagctgtat aagtgaagcg gccgcactcc tcaggtgcag
     2161 gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga
     2221 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact
     2281 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
     2341 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
     2401 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga
     2461 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg
     2521 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta
     2581 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc
     2641 atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct tggcgtaatc
     2701 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     2761 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     2821 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat
     2881 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     2941 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     3001 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     3061 ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     3121 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     3181 aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg gccaacgcgc
     3241 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     3301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     3721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     3901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     3961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     4021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     4081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     4141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     4201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     4261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     4321 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     4381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     4441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     4501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     4561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     4621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     4681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     4741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     4801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     4861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     4921 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     4981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     5041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcatatta ttgaagcatt
     5101 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     5161 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ggtcgacatt gattattgac
     5221 tagtaacccg tgtcggctcc agatctggcc tccgcgccgg gttttggcgc ctcccgcggg
     5281 cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga
     5341 tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc
     5401 ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt
     5461 ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg
     5521 atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct
     5581 agttccgtcg cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca
     5641 cttggtgagt agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt
     5701 gggacggaag cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg
     5761 ccctgaactg ggggttgggg gggagcgcag caaaatggcg gctgttcccg agtcttgaat
     5821 ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg catggtgggc
     5881 ggcaagaacc caaggtcttg aggccttcgc taatgcggga aagctcttat tcgggtgaga
     5941 tgggctgggg caccatctgg ggaccctgac gtgaagtttg tcactgactg gagaactcgg
     6001 tttgtcgtct gttgcggggg cggcagttat ggcggtgccg ttgggcagtg cacccgtacc
     6061 tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca
     6121 gggtggggcc acctgccggt aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt
     6181 tcgggcctag ggtaggctct cctgaatcga caggcgccgg acctctggtg aggggaggga
     6241 taagtgaggc gtcagtttct ttggtcggtt ttatgtacct atcttcttaa gtagctgaag
     6301 ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca
     6361 ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc agtgttagac tagtaaattg
     6421 tccgctaaat tctggccgtt tttggctttt ttgttagac
//