| 质粒名称: | pSicoR-eIF1a-GFP-RPL10a |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 159890 |
| 存储实验室: | James Ellis |
|
载体骨架基本信息 |
|
| 载体名称: | pSicoR-eIF1a-mCherry |
| 载体出品公司: | Clontech |
| 空载体大小: | 6909 bp |
| 完整质粒大小: | 8295bp |
| 载体类型: | Lentiviral |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | RPL10a |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1386bp |
| GenBank编号 | NM_007104.5 |
| 启动子: | IF1a |
| 标签/融合蛋白: | GFP (N terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | NheI (not destroyed) |
| 3’克隆位点: | EcoRI (destroyed during cloning) |
| 5’测序引物 | ACCGGTGCCTAGAGAAGGTGG |
| 3’测序引物 | CATAGCGTAAAAGGAGCAACA |
|
文献引用方法 |
|
| 材料和方法部分: | pSicoR-eIF1a-GFP-RPL10a was a gift from James Ellis (Addgene plasmid # 159890 ; http://n2t.net/addgene:159890 ; RRID:Addgene_159890) |
| 参考文献部分: | Shifts in Ribosome Engagement Impact Key Gene Sets in Neurodevelopment and Ubiquitination in Rett Syndrome. Rodrigues DC, Mufteev M, Weatheritt RJ, Djuric U, Ha KCH, Ross PJ, Wei W, Piekna A, Sartori MA, Byres L, Mok RSF, Zaslavsky K, Pasceri P, Diamandis P, Morris Q, Blencowe BJ, Ellis J. Cell Rep. 2020 Mar 24;30(12):4179-4196.e11. doi: 10.1016/j.celrep.2020.02.107. 10.1016/j.celrep.2020.02.107 PubMed 32209477 |
买家导航
pSicoR-eIF1a-GFP-RPL10a载体质粒基本信息
pSicoR-eIF1a-GFP-RPL10a质粒图谱载体图谱和pSicoR-eIF1a-GFP-RPL10a载体序列质粒序列多克隆位点信息
pSicoR-eIF1a-GFP-RPL10a质粒载体简介
pSicoR-eIF1a-GFP-RPL10a质粒序列载体序列
LOCUS pSicoR-eIF1a-GFP-RPL10a 8284 bp DNA circular SYN 14-APR-2025
DEFINITION Expresses GFP-RPL10a for ribosomal immunoprecipitation.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8284)
AUTHORS Rodrigues DC, Mufteev M, Weatheritt RJ, Djuric U, Ha KCH, Ross PJ,
Wei W, Piekna A, Sartori MA, Byres L, Mok RSF, Zaslavsky K, Pasceri
P, Diamandis P, Morris Q, Blencowe BJ, Ellis J
TITLE Shifts in Ribosome Engagement Impact Key Gene Sets in
Neurodevelopment and Ubiquitination in Rett Syndrome.
JOURNAL Cell Rep. 2020 Mar 24;30(12):4179-4196.e11. doi:
10.1016/j.celrep.2020.02.107.
PUBMED 32209477
REFERENCE 2 (bases 1 to 8284)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date:
"2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4179-4196.e11"
COMMENT Sequence Label: pSicoR-eIF1a-GFP-RPL10a
FEATURES Location/Qualifiers
source 1..8284
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(47..66)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 238..617
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 619..817
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 772..792
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 835..1015
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1062..1187
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1684..1917
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 2102..2146
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2444..2561
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
protein_bind complement(2814..2847)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
promoter 3007..3218
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 3231..3499
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
regulatory 3537..3546
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS join(3543..3545,3546..3548,3549..4259)
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS join(3543..3545,3546..3548,3549..4259)
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(3588..3609)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(3849..3868)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 4196..4217
/label=EGFP-C
/note="EGFP, forward primer"
protein_bind complement(5084..5117)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
misc_feature 5173..5761
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5226..5246)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5644..5655)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(5764..5780)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5765..5781)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 6290..6470
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
rep_origin complement(6532..7120)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6612..6631)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(7291..8082,8083..8151))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7914..7933
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8152..8256)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
2581 gcagagatcc agtttggtta gtaccgggcc cgctctagag atccgacgcg ccatctctag
2641 gcccgcgccg gccccctcgc acagacttgt gggagaagct cggctactcc cctgccccgg
2701 ttaatttgca tataatattt cctagtaact atagaggctt aatgtgcgat aaaagacaga
2761 taatctgttc tttttaatac tagctacatt ttacatgata ggcttggatt tctataactt
2821 cgtatagcat acattatacg aagttataca tgtcacaaaa ggaaactcac cctaactgta
2881 aagtaattgt gtgttttgag actataaata tcccttggag aaaagccttg ttaacgcgcg
2941 gtgaccctcg agtactagga tccattaggc ggccgcggat ctgcgatcgc tccggtgccc
3001 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
3061 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
3121 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
3181 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
3241 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
3301 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
3361 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
3421 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
3481 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagcgcta ccggtcgcca
3541 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
3601 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
3661 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
3721 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
3781 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
3841 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
3901 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
3961 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
4021 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
4081 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
4141 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
4201 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
4261 ccggccggac tcagatctcg agctcaagct tcgaattcag cagcaaagtc tcacgcgaca
4321 ccctgtacga ggcggtgcgg gaagtcctgc acgggaacca gcgcaagcgc cgcaagtttc
4381 tggagacggt ggagctgcag atcagcctga agaactacga ccctcagaag gacaaacgtt
4441 tctcgggcac cgtcaggctc aagtccaccc cacgccccaa gttctcggtg tgcgttctgg
4501 gggaccagca gcactgtgat gaagccaagg ccgtggatat cccccacatg gacatcgagg
4561 cgctcaagaa gcttaacaaa aacaagaagt tggtcaagaa gctggctaag aagtacgatg
4621 cctttttggc ctctgagtct ctgattaagc agatcccacg tatcctgggc ccaggcctaa
4681 acaaggctgg caagttcccc tccctgctga cacacaatga aaacatggtg gccaaagtgg
4741 atgaggtgaa atcgacaatc aagttccaga tgaagaaggt gctgtgtttg gccgtcgctg
4801 ttggccacgt gaagatgacc gatgatgagc tagtctacaa cattcatctg gctgtcaatt
4861 tcttggtgtc cttgcttaag aaaaactggc aaaacgtgcg ggctctgtac atcaagagca
4921 ccatgggcaa gccccagcgt ctgtattagg gatccaccgg atctagataa ctgatcataa
4981 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
5041 tgaacctgaa acataaaatg aatgcaattc gtcgagggac ctaataactt cgtatagcat
5101 acattatacg aagttataca tgtttaaggg ttccggttcc actaggtaca attcgatatc
5161 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
5221 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
5281 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
5341 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
5401 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
5461 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
5521 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
5581 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
5641 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
5701 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
5761 catcgatacc gtcgacctcg atcgagacct agaaaaacat ggagcaatca caagtagcaa
5821 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
5881 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
5941 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
6001 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta
6061 cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc
6121 agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc
6181 tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag
6241 ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg
6301 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
6361 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
6421 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gcatgtgagc
6481 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
6541 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
6601 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
6661 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
6721 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
6781 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
6841 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
6901 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
6961 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
7021 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
7081 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
7141 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
7201 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
7261 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
7321 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
7381 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
7441 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
7501 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
7561 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
7621 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
7681 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
7741 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
7801 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
7861 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
7921 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
7981 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
8041 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
8101 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
8161 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
8221 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
8281 tgac
//
