| 质粒名称: | pXY1-spc |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 90488 |
| 存储实验室: | Sheng Yang |
|
载体骨架基本信息 |
|
| 载体名称: | pXY1 |
| 完整质粒大小: | 5286 bp |
| 载体类型: | 细菌表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | 无 |
|
文献引用方法 |
|
| 材料和方法部分: | pXY1-spc was a gift from Sheng Yang (Addgene plasmid # 90488 ; http://n2t.net/addgene:90488 ; RRID:Addgene_90488) |
| 参考文献部分: | I-SceI-mediated scarless gene modification via allelic exchange in Clostridium. Zhang N, Shao L, Jiang Y, Gu Y, Li Q, Liu J, Jiang W, Yang S. J Microbiol Methods. 2015 Jan;108:49-60. doi: 10.1016/j.mimet.2014.11.004. Epub 2014 Nov 15. 10.1016/j.mimet.2014.11.004 PubMed 25451462 |
买家导航
pXY1-spc载体质粒基本信息
pXY1-spc质粒图谱载体图谱和pXY1-spc载体序列质粒序列多克隆位点信息
pXY1-spc质粒载体简介
pXY1-spc质粒序列载体序列
LOCUS pXY1-spc 5286 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Plasmid with ptb promoter for gene expression in Clostridium
acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052.
ACCESSION .
VERSION .
KEYWORDS pXY1-spc
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5286)
AUTHORS Zhang N, Shao L, Jiang Y, Gu Y, Li Q, Liu J, Jiang W, Yang S
TITLE I-SceI-mediated scarless gene modification via allelic exchange in
Clostridium.
JOURNAL J Microbiol Methods. 2015 Jan;108:49-60. doi:
10.1016/j.mimet.2014.11.004. Epub 2014 Nov 15.
PUBMED 25451462
REFERENCE 2 (bases 1 to 5286)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5286
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin complement(126..714)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(206..225)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(885..1745)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1508..1527
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1746..1850)
/gene="bla"
/label=AmpR promoter
primer_bind 1918..1936
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(1974..1996)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 2096..2115
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(5010..5026)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5010..5026)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5023..5045)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5034..5050
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5058..5088)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5103..5124
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5241..5258)
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
61 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
121 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
181 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
241 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
301 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
361 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
421 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
481 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
541 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
601 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
661 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
721 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
781 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
841 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
901 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
961 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
1021 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
1081 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
1141 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
1201 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
1261 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
1321 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
1381 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
1441 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
1501 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
1561 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
1621 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
1681 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
1741 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
1801 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
1861 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
1921 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
1981 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
2041 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
2101 tcagagcaga ttgtactgag agtgcaccat atgtcgacac tcccttttac tatttaatta
2161 tctatgttaa atgattaacg tgctcattta tattttaaca aacttttcac atgaagttaa
2221 gttttttaga aaattattta tattttatac tgcagcacat taagtatata ctattattaa
2281 actattctat atacttaatt tattttaata gaaaaacata atatcataat aacttcaaaa
2341 ttaaacttta tttatgattt catacttgac tttgatttta gaaaggatat actttttagc
2401 agatttggaa acggctttgg acgtagtttg cccatagatg aacaaacaaa ctacatccaa
2461 aaattatact tttcccttca ttggtatccg tatttttaca tcttaatagc gtatgtatta
2521 caacacacct aaacaacgac cttacggtct gctactgcat atcctagctt gattgtttag
2581 ttgcctcaac tatgcttaac cctaccccga actctttttt tattgtgggt tttcgtcgtg
2641 aagtcccacc gacacataat cataacataa gatgtattat gaaaatgcga gtgactatcc
2701 ttttgtatcg gctcactaca ccacagatat attttttagt gcatactgtg tcggcactct
2761 caatattaaa ttaataaata aattattttt tctttttact cttctttaca tgagcttttt
2821 taaagctcct tgcataatat ttaatgcatg tacgttcttt tttctgttct tcctctgtaa
2881 aacatctcat ttttatggca caccatccat atcggttcat ctttgaacaa ttaatacatt
2941 ggactttccc tttatgtaaa catcttgact cattgtattt actgcaatag gttgctactg
3001 ttttatcatg aatcatcaac gaatgtattt tgcatatttc agtatcttta ataaactctt
3061 tgcaattttt acaagttttc atatacgccc ttcttttcat aaattaattt atgaattcta
3121 tgtattccaa ggagcttttt aaagctcccc ctttcgtact acttagctac aagcactata
3181 aaagtcataa tgtttactgc taaagtcaat aatgatattg ctaatacctt gttatttgat
3241 aagatactgc tttcctctgt cactttgctc accccctttc attttcataa attaattatg
3301 aaaaaataaa tatacttcta atttttatca aataaaaaag cctttgcgta ctgcttccaa
3361 tacacaaagg ctataaactt ctaaatctta cttattgcaa tttacattta tatctgttaa
3421 gataatctca taaattgaat atatatagtt aatgtttctc ttgtatgtcg gtacatttga
3481 aatattgcta tagatagagt tctctaacgg cttgatgtgt tggtagcaca tttaagtttt
3541 ggcttatata ctaagttggt agcttaatat ataagagctg aggacttatt tttttattaa
3601 atttttcaac ttgtctatat tttaacccgt aattgaatac ataacaagta tttttttttg
3661 tattcaatta aacattcata aatgagtata attaatcata ctaaattcta taattttctt
3721 ttctgtaaat tatcgattaa tttttttaat ctgttattta aatagtttat agttaaattt
3781 acattttcat tagtccattc aatattctct ccaagataac tacgaactgc taacaaaatt
3841 ctctccctat gttctaatgg agaagattca gccactgcat ttcccgcaat atcttttggt
3901 atgattttac ccgtgtccat agttaaaatc atacggcata aagttaatat agagttggtt
3961 tcatcatcct gataattatc tattaattcc tctgacgaat ccataatggc tcttctcaca
4021 tcagaaaatg gaatatcagg tagtaattcc tctaagtcat aatttccgta tattctttta
4081 ttttttcgtt ttgcttggta aagcattatg gttaaatctg aatttaattc cttctgagga
4141 atgtatcctt gttcataaag ctcttgtaac cattctccat aaataaattc ttgtttggga
4201 ggatgattcc acggtaccat ttcttgctga ataataattg ttaattcaat atatcgtaag
4261 ttgcttttat ctcctatttt ttttgaaata ggtctaattt tttgtataag tatttcttta
4321 ctttgatctg tcaatggttc agatacgacg actaaaaagt caagatcact atttggtttt
4381 agtccactct caactcctga tccaaacatg taagtaccaa taaggttatt ttttaaatgt
4441 ttccgaagta tttttttcac tttattaatt tgttcgtatg tattcaaata tatcctcctc
4501 actattttga ttagtaccta ttttatatcc atagttgtta attaaataaa cttaatttag
4561 tttatttata gatttcattg gcttctaaat tttttatcta gataataatt attttagtta
4621 attttattct agattatata tgatatgatc tttcatttcc ataaaactaa agtaagtgta
4681 aacctattca ttgtggctct gataaatatg aacatgatga gtgatcgtta aatttatact
4741 gcaatctgat gcgattattg aataaaagat atgagagatt tatctagttt ctttttttac
4801 aagaaaaaag aaagttctta aaggttttat acttttggtc gtagagcaca cggtttaacg
4861 acttaattac gaagtaaata agtctagtgt gttagacttt aatgtttttt taaggcatta
4921 gtgcatttaa gcgtcagagc atggctttat gccgagaaaa ctattggttg gaatggcgtg
4981 tgtgttagcc aaagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
5041 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
5101 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
5161 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
5221 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
5281 cgagcg
//
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