| 质粒名称: | pYK237 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000807 |
| 产品用途: | Mammalian PhCMV-driven constitutive dCas9-VPR-4xANR expression vector (PhCMV-dCas9-VPR-4xANR-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | dCas9-VPR-4xANR |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK237
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK237载体质粒基本信息
pYK237质粒图谱载体图谱和pYK237载体序列质粒序列多克隆位点信息
pYK237质粒载体简介
pYK237质粒序列载体序列
LOCUS pYK237 11803 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11803)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 27, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..11803
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 881..919
/label=oYK207_dcas9_F
CDS 920..6715
/label=Translation 748-6546
CDS 920..5023
/label=dCas9 (D839A and N863A nonsense-mutation)
primer_bind complement(5034..5056)
/label=oYK497_dCas9_2R
misc_feature 5036..5056
/label=NLS (SV40 short)
/label=nonstandard type: signal
primer_bind complement(5069..5092)
/label=oYK497_dCas9_R
misc_recomb 5094..5114
/label=attB1
misc_feature 5147..6715
/label=VPR
/label=nonstandard type: AD
CDS 5147..5296
/label=VP64
misc_feature 5147..5185
/label=nonstandard type: AD
misc_feature 5186..5224
/label=nonstandard type: AD
misc_feature 5225..5263
/label=nonstandard type: AD
primer_bind complement(5315..5341)
/label=oYK207_dcas9VP64_R
misc_feature 5321..5341
/label=NLS (SV40 short)
/label=nonstandard type: signal
misc_feature 5771..6127
/label=RelA (p65) AD
/label=nonstandard type: AD
CDS 6146..6715
/label=Rta AD
CDS 6146..6715
/label=Translation 5974-6543
primer_bind complement(6691..6735)
/label=oYK237_dCas9-VPR_R
primer_bind 6715..6735
/label=oSCL1129
primer_bind 6715..6733
/label=oLZ147 NS3a F
primer_bind 6715..6732
/label=oYF0033
misc_feature 6716..6751
/label=linker
primer_bind 6716..6745
/label=oSCL388
misc_feature 6716..6736
/label=linker
primer_bind 6716..6735
/label=oLZ048 NSP3-F
primer_bind complement(6716..6735)
/label=oYK497_dCas9_R
primer_bind complement(6716..6735)
/label=oYK497_dCas9_2R
primer_bind 6716..6731
/label=oYK28-mCherry-NS3a-F
primer_bind complement(6726..6751)
/label=oSCL1119
primer_bind complement(6726..6745)
/label=oSCL075
primer_bind complement(6730..6765)
/label=oYK92_L7Ae_R
primer_bind complement(6734..6752)
/label=oYK75_NLS_mcp_R
primer_bind complement(6740..6794)
/label=oSCL388
CDS 6746..6820
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind complement(6746..6791)
/label=oYK215_Mcp_B_R
primer_bind complement(6746..6765)
/label=oYK195_ANR_R
primer_bind 6746..6760
/label=oYK82_LinkANR_F
primer_bind complement(6747..6766)
/label=test2
CDS 6757..6764
/codon_start=1
/label=NS3a H1
/translation="GS"
primer_bind 6772..6791
/label=oYK215_ANR1rep_F
primer_bind complement(6772..6791)
/label=oYK215_ANR1rep_R
primer_bind 6801..6820
/label=test1
primer_bind complement(6804..6820)
/label=TEST3
CDS 6821..6883
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 6821..6836
/label=Primer 4
primer_bind complement(6860..6903)
/label=oYK195_ANR_R
primer_bind complement(6865..6883)
/label=oYK82_LinkANR_R
primer_bind 6868..6882
/label=Primer 3
primer_bind complement(6879..6929)
/label=oYK215_Mcp_B_R
CDS 6884..6958
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind complement(6884..6903)
/label=oYK92_L7Ae_R
primer_bind 6884..6898
/label=oYK82_LinkANR_F
primer_bind complement(6885..6904)
/label=test2
primer_bind 6910..6929
/label=oYK215_ANR1rep_F
primer_bind complement(6910..6929)
/label=oYK215_ANR1rep_R
primer_bind 6939..6958
/label=test1
primer_bind complement(6942..6958)
/label=TEST3
CDS 6959..7021
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 6959..6974
/label=Primer 4
primer_bind complement(6998..7041)
/label=oYK195_ANR_R
primer_bind complement(7003..7021)
/label=oYK82_LinkANR_R
primer_bind 7006..7020
/label=Primer 3
primer_bind complement(7017..7067)
/label=oYK215_Mcp_B_R
CDS 7022..7096
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind complement(7022..7041)
/label=oYK92_L7Ae_R
primer_bind 7022..7036
/label=oYK82_LinkANR_F
primer_bind complement(7023..7042)
/label=test2
primer_bind 7048..7067
/label=oYK215_ANR1rep_F
primer_bind complement(7048..7067)
/label=oYK215_ANR1rep_R
primer_bind 7077..7096
/label=test1
primer_bind complement(7080..7096)
/label=TEST3
CDS 7097..7159
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 7097..7112
/label=Primer 4
primer_bind complement(7136..7179)
/label=oYK195_ANR_R
primer_bind complement(7141..7159)
/label=oYK82_LinkANR_R
primer_bind 7144..7158
/label=Primer 3
primer_bind complement(7155..7205)
/label=oYK215_Mcp_B_R
CDS 7160..7234
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind complement(7160..7179)
/label=oYK92_L7Ae_R
primer_bind 7160..7174
/label=oYK82_LinkANR_F
primer_bind complement(7161..7180)
/label=test2
primer_bind 7186..7205
/label=oYK215_ANR1rep_F
primer_bind complement(7186..7205)
/label=oYK215_ANR1rep_R
primer_bind 7215..7234
/label=test1
primer_bind complement(7218..7234)
/label=TEST3
CDS 7235..7297
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 7235..7250
/label=Primer 4
primer_bind complement(7274..7297)
/label=oYK195_ANR_R
primer_bind complement(7279..7320)
/label=oYK82_LinkANR_R
primer_bind 7282..7296
/label=Primer 3
primer_bind complement(7301..7323)
/label=oYK57-MCP-mCherry-R
primer_bind complement(7301..7320)
/label=oYK207_dcas9VP64_R
terminator 7397..7624
/label=bGH PA
misc_feature 7670..8098
/label=f1 origin
misc_feature 8103..8446
/label=SV40 early promoter and origin
misc_feature 8292..8369
/label=SV40 ORI
CDS 8511..9302
/label=Kan/neoR
misc_feature 9476..9606
/label=SV40 early polyadenylation signal
primer_bind complement(9642..9662)
/label=M13-rev
misc_binding complement(9668..9690)
/label=LacO
promoter complement(9695..9724)
/label=lac
misc_feature complement(9989..10662)
/label=pUC origin
CDS complement(10810..11469)
/label=AmpR
promoter complement(11709..11737)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 ggatcgaacc cttgccacca tggacaagaa gtactccatt gggctcgcta tcggcacaaa
961 cagcgtcggc tgggccgtca ttacggacga gtacaaggtg ccgagcaaaa aattcaaagt
1021 tctgggcaat accgatcgcc acagcataaa gaagaacctc attggcgccc tcctgttcga
1081 ctccggggag acggccgaag ccacgcggct caaaagaaca gcacggcgca gatatacccg
1141 cagaaagaat cggatctgct acctgcagga gatctttagt aatgagatgg ctaaggtgga
1201 tgactctttc ttccataggc tggaggagtc ctttttggtg gaggaggata aaaagcacga
1261 gcgccaccca atctttggca atatcgtgga cgaggtggcg taccatgaaa agtacccaac
1321 catatatcat ctgaggaaga agcttgtaga cagtactgat aaggctgact tgcggttgat
1381 ctatctcgcg ctggcgcata tgatcaaatt tcggggacac ttcctcatcg agggggacct
1441 gaacccagac aacagcgatg tcgacaaact ctttatccaa ctggttcaga cttacaatca
1501 gcttttcgaa gagaacccga tcaacgcatc cggagttgac gccaaagcaa tcctgagcgc
1561 taggctgtcc aaatcccggc ggctcgaaaa cctcatcgca cagctccctg gggagaagaa
1621 gaacggcctg tttggtaatc ttatcgccct gtcactcggg ctgaccccca actttaaatc
1681 taacttcgac ctggccgaag atgccaagct tcaactgagc aaagacacct acgatgatga
1741 tctcgacaat ctgctggccc agatcggcga ccagtacgca gacctttttt tggcggcaaa
1801 gaacctgtca gacgccattc tgctgagtga tattctgcga gtgaacacgg agatcaccaa
1861 agctccgctg agcgctagta tgatcaagcg ctatgatgag caccaccaag acttgacttt
1921 gctgaaggcc cttgtcagac agcaactgcc tgagaagtac aaggaaattt tcttcgatca
1981 gtctaaaaat ggctacgccg gatacattga cggcggagca agccaggagg aattttacaa
2041 atttattaag cccatcttgg aaaaaatgga cggcaccgag gagctgctgg taaagcttaa
2101 cagagaagat ctgttgcgca aacagcgcac tttcgacaat ggaagcatcc cccaccagat
2161 tcacctgggc gaactgcacg ctatcctcag gcggcaagag gatttctacc cctttttgaa
2221 agataacagg gaaaagattg agaaaatcct cacatttcgg ataccctact atgtaggccc
2281 cctcgcccgg ggaaattcca gattcgcgtg gatgactcgc aaatcagaag agaccatcac
2341 tccctggaac ttcgaggaag tcgtggataa gggggcctct gcccagtcct tcatcgaaag
2401 gatgactaac tttgataaaa atctgcctaa cgaaaaggtg cttcctaaac actctctgct
2461 gtacgagtac ttcacagttt ataacgagct caccaaggtc aaatacgtca cagaagggat
2521 gagaaagcca gcattcctgt ctggagagca gaagaaagct atcgtggacc tcctcttcaa
2581 gacgaaccgg aaagttaccg tgaaacagct caaagaagac tatttcaaaa agattgaatg
2641 tttcgactct gttgaaatca gcggagtgga ggatcgcttc aacgcatccc tgggaacgta
2701 tcacgatctc ctgaaaatca ttaaagacaa ggacttcctg gacaatgagg agaacgagga
2761 cattcttgag gacattgtcc tcacccttac gttgtttgaa gatagggaga tgattgaaga
2821 acgcttgaaa acttacgctc atctcttcga cgacaaagtc atgaaacagc tcaagaggcg
2881 ccgatataca ggatgggggc ggctgtcaag aaaactgatc aatgggatcc gagacaagca
2941 gagtggaaag acaatcctgg attttcttaa gtccgatgga tttgccaacc ggaacttcat
3001 gcagttgatc catgatgact ctctcacctt taaggaggac atccagaaag cacaagtttc
3061 tggccagggg gacagtcttc acgagcacat cgctaatctt gcaggtagcc cagctatcaa
3121 aaagggaata ctgcagaccg ttaaggtcgt ggatgaactc gtcaaagtaa tgggaaggca
3181 taagcccgag aatatcgtta tcgagatggc ccgagagaac caaactaccc agaagggaca
3241 gaagaacagt agggaaagga tgaagaggat tgaagagggt ataaaagaac tggggtccca
3301 aatccttaag gaacacccag ttgaaaacac ccagcttcag aatgagaagc tctacctgta
3361 ctacctgcag aacggcaggg acatgtacgt ggatcaggaa ctggacatca atcggctctc
3421 cgactacgac gtggctgcta tcgtgcccca gtcttttctc aaagatgatt ctattgataa
3481 taaagtgttg acaagatccg ataaagctag agggaagagt gataacgtcc cctcagaaga
3541 agttgtcaag aaaatgaaaa attattggcg gcagctgctg aacgccaaac tgatcacaca
3601 acggaagttc gataatctga ctaaggctga acgaggtggc ctgtctgagt tggataaagc
3661 cggcttcatc aaaaggcagc ttgttgagac acgccagatc accaagcacg tggcccaaat
3721 tctcgattca cgcatgaaca ccaagtacga tgaaaatgac aaactgattc gagaggtgaa
3781 agttattact ctgaagtcta agctggtctc agatttcaga aaggactttc agttttataa
3841 ggtgagagag atcaacaatt accaccatgc gcatgatgcc tacctgaatg cagtggtagg
3901 cactgcactt atcaaaaaat atcccaagct tgaatctgaa tttgtttacg gagactataa
3961 agtgtacgat gttaggaaaa tgatcgcaaa gtctgagcag gaaataggca aggccaccgc
4021 taagtacttc ttttacagca atattatgaa ttttttcaag accgagatta cactggccaa
4081 tggagagatt cggaagcgac cacttatcga aacaaacgga gaaacaggag aaatcgtgtg
4141 ggacaagggt agggatttcg cgacagtccg gaaggtcctg tccatgccgc aggtgaacat
4201 cgttaaaaag accgaagtac agaccggagg cttctccaag gaaagtatcc tcccgaaaag
4261 gaacagcgac aagctgatcg cacgcaaaaa agattgggac cccaagaaat acggcggatt
4321 cgattctcct acagtcgctt acagtgtact ggttgtggcc aaagtggaga aagggaagtc
4381 taaaaaactc aaaagcgtca aggaactgct gggcatcaca atcatggagc gatcaagctt
4441 cgaaaaaaac cccatcgact ttctcgaggc gaaaggatat aaagaggtca aaaaagacct
4501 catcattaag cttcccaagt actctctctt tgagcttgaa aacggccgga aacgaatgct
4561 cgctagtgcg ggcgagctgc agaaaggtaa cgagctggca ctgccctcta aatacgttaa
4621 tttcttgtat ctggccagcc actatgaaaa gctcaaaggg tctcccgaag ataatgagca
4681 gaagcagctg ttcgtggaac aacacaaaca ctaccttgat gagatcatcg agcaaataag
4741 cgaattctcc aaaagagtga tcctcgccga cgctaacctc gataaggtgc tttctgctta
4801 caataagcac agggataagc ccatcaggga gcaggcagaa aacattatcc acttgtttac
4861 tctgaccaac ttgggcgcgc ctgcagcctt caagtacttc gacaccacca tagacagaaa
4921 gcggtacacc tctacaaagg aggtcctgga cgccacactg attcatcagt caattacggg
4981 gctctatgaa acaagaatcg acctctctca gctcggtgga gacagcaggg ctgaccccaa
5041 gaagaagagg aaggtgtcgc cagggatccg tcgacttgac gcgttgatat caacaagttt
5101 gtacaaaaaa gcaggctaca aagaggccag cggttccgga cgggctgacg cattggacga
5161 ttttgatctg gatatgctgg gaagtgacgc cctcgatgat tttgaccttg acatgcttgg
5221 ttcggatgcc cttgatgact ttgacctcga catgctcggc agtgacgccc ttgatgattt
5281 cgacctggac atgctgatta actctagaag ttccggatct ccgaaaaaga aacgcaaagt
5341 tggtagccag tacctgcccg acaccgacga ccggcaccgg atcgaggaaa agcggaagcg
5401 gacctacgag acattcaaga gcatcatgaa gaagtccccc ttcagcggcc ccaccgaccc
5461 tagacctcca cctagaagaa tcgccgtgcc cagcagatcc agcgccagcg tgccaaaacc
5521 tgccccccag ccttacccct tcaccagcag cctgagcacc atcaactacg acgagttccc
5581 taccatggtg ttccccagcg gccagatctc tcaggcctct gctctggctc cagcccctcc
5641 tcaggtgctg cctcaggctc ctgctcctgc accagctcca gccatggtgt ctgcactggc
5701 tcaggcacca gcacccgtgc ctgtgctggc tcctggacct ccacaggctg tggctccacc
5761 agcccctaaa cctacacagg ccggcgaggg cacactgtct gaagctctgc tgcagctgca
5821 gttcgacgac gaggatctgg gagccctgct gggaaacagc accgatcctg ccgtgttcac
5881 cgacctggcc agcgtggaca acagcgagtt ccagcagctg ctgaaccagg gcatccctgt
5941 ggcccctcac accaccgagc ccatgctgat ggaatacccc gaggccatca cccggctcgt
6001 gacaggcgct cagaggcctc ctgatccagc tcctgcccct ctgggagcac caggcctgcc
6061 taatggactg ctgtctggcg acgaggactt cagctctatc gccgatatgg atttctcagc
6121 cttgctgggc tctggcagcg gcagccggga ttccagggaa gggatgtttt tgccgaagcc
6181 tgaggccggc tccgctatta gtgacgtgtt tgagggccgc gaggtgtgcc agccaaaacg
6241 aatccggcca tttcatcctc caggaagtcc atgggccaac cgcccactcc ccgccagcct
6301 cgcaccaaca ccaaccggtc cagtacatga gccagtcggg tcactgaccc cggcaccagt
6361 ccctcagcca ctggatccag cgcccgcagt gactcccgag gccagtcacc tgttggagga
6421 tcccgatgaa gagacgagcc aggctgtcaa agcccttcgg gagatggccg atactgtgat
6481 tccccagaag gaagaggctg caatctgtgg ccaaatggac ctttcccatc cgcccccaag
6541 gggccatctg gatgagctga caaccacact tgagtccatg accgaggatc tgaacctgga
6601 ctcacccctg accccggaat tgaacgagat tctggatacc ttcctgaacg acgagtgcct
6661 cttgcatgcc atgcatatca gcacaggact gtccatcttc gacacatctc tgtttgccag
6721 cggaagtggc ggaggaggcg acgtcggctc cggaacgggc agcgggaccg gaagtggcac
6781 cggaaccact tccggaaccg gtactggcgg gagtaccggc ggtgagctag acgaactcgt
6841 gtacctgttg gatggccccg gttacgaccc aatccacagc gatggctccg gaacgggcag
6901 cgggaccgga agtggcaccg gaaccacttc cggaaccggt actggcggga gtaccggcgg
6961 tgagctagac gaactcgtgt acctgttgga tggccccggt tacgacccaa tccacagcga
7021 tggctccgga acgggcagcg ggaccggaag tggcaccgga accacttccg gaaccggtac
7081 tggcgggagt accggcggtg agctagacga actcgtgtac ctgttggatg gccccggtta
7141 cgacccaatc cacagcgatg gctccggaac gggcagcggg accggaagtg gcaccggaac
7201 cacttccgga accggtactg gcgggagtac cggcggtgag ctagacgaac tcgtgtacct
7261 gttggatggc cccggttacg acccaatcca cagcgattaa ggatccacta gtccagtgtg
7321 gtggaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg gcccgtttaa
7381 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
7441 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
7501 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
7561 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
7621 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
7681 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
7741 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
7801 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
7861 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
7921 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
7981 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
8041 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
8101 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
8161 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
8221 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
8281 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
8341 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
8401 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
8461 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
8521 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
8581 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
8641 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
8701 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
8761 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
8821 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
8881 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
8941 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
9001 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
9061 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
9121 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
9181 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
9241 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
9301 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
9361 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
9421 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
9481 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
9541 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
9601 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
9661 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
9721 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
9781 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
9841 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
9901 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
9961 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
10021 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
10081 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
10141 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
10201 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
10261 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
10321 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
10381 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
10441 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
10501 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
10561 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
10621 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
10681 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
10741 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
10801 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
10861 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
10921 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
10981 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
11041 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
11101 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
11161 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
11221 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
11281 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
11341 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
11401 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
11461 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
11521 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
11581 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
11641 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
11701 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
11761 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//
pYK237载体图谱质粒图谱pdf版和pYK237载体序列质粒序列等相关资料下载
pYK237质粒载体应用举例
- 上一篇：pYK231
- 下一篇：没有了
