pLV-EF1a-MinE-EGFP_IRES-Blast

LOCUS       pLV-EF1a-MinE-EGFP_IRES-Blast                9545 bp ds-DNA     circular SYN 26-JAN-2024
DEFINITION  Expression of MinE-EGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pLV-EF1a-MinE-EGFP_IRES-Blast
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9545)
  AUTHORS   Rajasekaran R, Chang CC, Weix EWZ, Galateo TM, Coyle SM
  TITLE     A programmable reaction-diffusion system for spatiotemporal cell 
            signaling circuit design.
  JOURNAL   Cell. 2023 Dec 27:S0092-8674(23)01339-9. doi: 
            10.1016/j.cell.2023.12.007.
  PUBMED    38181787
REFERENCE   2  (bases 1 to 9545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 26, 2024 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9545
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    591..1179
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(644..664)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1062..1073)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1386..2019
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2147..2163)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2147..2163)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2160..2182)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2171..2187
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2195..2225)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2240..2261
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2378..2395)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2549..3137)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2629..2648)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3308..4168)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3931..3950
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4169..4273)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    4321..4455
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4358..4377)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4412..4431
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             4623..5256
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5303..5428
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5925..6158
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6343..6387
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6650..6765
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        6843..7054
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          7055..7993
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7950..7970
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      8009..8018
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             8312..9031
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             8312..9028
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8357..8378)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8618..8637)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8965..8986
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(9276..9293)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9503..9522
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
       61 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
      121 gaaaaacacg ataataccat ggtcatgaaa acatttaaca tttctcaaca agatctagaa
      181 ttagtagaag tagcgacaga gaagattaca atgctttatg aggataataa acatcatgtg
      241 ggagcggcaa ttcgtacgaa aacaggagaa atcatttcgg cagtacatat tgaagcgtat
      301 ataggacgag taactgtttg tgcagaagcc attgcgattg gtagtgcagt ttcgaatgga
      361 caaaaggatt ttgacacgat tgtagctgtt agacaccctt attctgacga agtagataga
      421 agtattcgag tggtaagtcc ttgtggtatg tgtagggagt tgatttcaga ctatgcacca
      481 gattgttttg tgttaataga aatgaatggc aagttagtca aaactacgat tgaagaactc
      541 attccactca aatatacccg aaattaaccc gggcggggcg cgtctggaac aatcaacctc
      601 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
      661 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
      721 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
      781 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
      841 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
      901 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
      961 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     1021 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     1081 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     1141 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg
     1201 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     1261 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     1321 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag
     1381 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     1441 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     1501 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     1561 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     1621 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     1681 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     1741 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     1801 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     1861 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     1921 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     1981 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     2041 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt
     2101 gctagcgttt accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     2161 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     2221 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     2281 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     2341 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     2401 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     2461 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     2521 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2581 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2641 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2701 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2761 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2821 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2881 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2941 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     3001 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     3061 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     3121 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3181 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     3241 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     3301 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     3361 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     3421 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     3481 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     3541 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     3601 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     3661 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     3721 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     3781 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     3841 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     3901 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     3961 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     4021 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     4081 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     4141 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     4201 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     4261 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     4321 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     4381 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     4441 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
     4501 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
     4561 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
     4621 gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca
     4681 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc
     4741 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc
     4801 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     4861 ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg
     4921 agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc
     4981 cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag
     5041 atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct
     5101 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5161 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     5221 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     5281 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     5341 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     5401 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     5461 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     5521 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     5581 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     5641 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     5701 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     5761 gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     5821 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     5881 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     5941 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     6001 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     6061 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     6121 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     6181 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     6241 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     6301 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     6361 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     6421 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     6481 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     6541 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     6601 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcct ttaaaagaaa
     6661 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     6721 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     6781 ggacagcaga gatccagttt atcgatgagg ccctttcgtc ttcactcgag gtgcccgtca
     6841 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     6901 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     6961 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     7021 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     7081 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     7141 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     7201 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     7261 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     7321 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     7381 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     7441 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     7501 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     7561 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     7621 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     7681 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     7741 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     7801 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     7861 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     7921 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     7981 tcttccattt caggtgtcgt gaggatccgc caccatggcc cttcttgact tcttcctgtc
     8041 tcgaaaaaag aacacagcga atatcgcgaa agaacggttg caaattatag tggcggagag
     8101 acgacgaagc gacgcggagc ctcattacct tcctcaattg cgaaaagaca tactggaagt
     8161 gatctgcaag tacgttcaaa ttgaccctga gatggtgacc gtgcaacttg aacagaagga
     8221 tggagatata agcatcctgg aattgaacgt aacccttccc gaggcggaag aacttaaaga
     8281 cagcgccgga agtgctggct ctgcgggtac catggtgagc aagggcgagg agctgttcac
     8341 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     8401 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     8461 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     8521 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     8581 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     8641 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     8701 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     8761 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     8821 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     8881 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     8941 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     9001 cactctcggc atggacgagc tgtacaagta agcggccgct ctagagtcga cgggccgcgg
     9061 taacaattgt taactaactt aagctagcaa cggtttccct ctagcgggat caattccgcc
     9121 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     9181 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     9241 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     9301 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     9361 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     9421 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     9481 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     9541 tgccc
//