| 质粒名称: | pAAVLINK-3'-ANKRD11-FLAG-bGHpA |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001925 |
| 产品用途: | 3' AAVLINK plasmid for ANKRD11 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | Promoterless |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ANKRD11 |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-3'-ANKRD11-FLAG-bGHpA
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-3'-ANKRD11-FLAG-bGHpA载体质粒基本信息
pAAVLINK-3'-ANKRD11-FLAG-bGHpA质粒图谱载体图谱和pAAVLINK-3'-ANKRD11-FLAG-bGHpA载体序列质粒序列多克隆位点信息
pAAVLINK-3'-ANKRD11-FLAG-bGHpA质粒载体简介
pAAVLINK-3'-ANKRD11-FLAG-bGHpA质粒序列载体序列
LOCUS pAAVLINK-3'-ANKRD11-FLAG-bGHpA 7315 bp DNA circular SYN 10-AUG-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7315)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 16, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7315
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
protein_bind 145..178
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 179..270
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 271..4255
/codon_start=1
/gene="ANKRD11"
/gene_synonym="ANCO-1; ANCO1; LZ16; T13"
/product="ankyrin repeat domain-containing protein 11"
/label=ANKRD11
/note="nasopharyngeal carcinoma susceptibility
protein;ankyrin repeat-containing cofactor 1; ankyrin
repeat domain 11; ankyrin repeats containing cofactor 1"
/db_xref="GI:56676397"
/db_xref="CCDS:CCDS32513.1"
/db_xref="GeneID:29123"
/db_xref="HGNC:HGNC:21316"
/db_xref="MIM:611192"
/protein_id="NP_037407.4"
/translation="ESACLPEKLKEKERHRHSSSSSKKSHDRERAKKEKAEKKEKGEDY
KEGGSRKDSGQYEKDFLEADAYGVSYNMKADIEDELDKTIELFSTEKKDKNDSEREPSK
KIEKELKPYGSSAINILKEKKKREKHREKWRDEKERHRDRHADGLLRHHRDELLRHHRD
EQKPATRDKDSPPRVLKDKSRDEGPRLGDAKLKEKFKDGAEKEKGDPVKMSNGNDKVAP
SKDPGKKDARPREKLLGDGDLMMTSFERMLSQKDLEIEERHKRHKERMKQMEKLRHRSG
DPKLKEKAKPADDGRKKGLDIPAKKPPGLDPPFKDKKLKESTPIPPAAENKLHPASGAD
SKDWLAGPHMKEVLPASPRPDQSRPTGVPTPTSVLSCPSYEEVMHTPRTPSCSADDYAD
LVFDCADSQHSTPVPTAPTSACSPSFFDRFSVASSGLSENASQAPARPLSTNLYRSVSV
DIRRTPEEEFSVGDKLFRQQSVPAASSYDSPMPPSMEDRAPLPPVPAEKFACLSPGYYS
PDYGLPSPKVDALHCPPAAVVTVTPSPEGVFSSLQAKPSPSPRAELLVPSLEGALPPDL
DTSEDQQATAAIIPPEPSYLEPLDEGPFSAVITEEPVEWAHPSEQALASSLIGGTSENP
VSWPVGSDLLLKSPQRFPESPKRFCPADPLHSAAPGPFSASEAPYPAPPASPAPYALPV
AEPGLEDVKDGVDAVPAAISTSEAAPYAPPSGLESFFSNCKSLPEAPLDVAPEPACVAA
VAQVEALGPLENSFLDGSRGLSHLGQVEPVPWADAFAGPEDDLDLGPFSLPELPLQTKD
AADGEAEPVEESLAPPEEMPPGAPGVINGGDVSTVVAEEPPALPPDQASTRLPAELEPE
PSGEPKLDVALEAAVEAETVPEERARGDPDSSVEPAPVPPEQRPLGSGDQGAEAEGPPA
ASLCAPDGPAPNTVAQAQAADGAGPEDDTEASRAAAPAEGPPGGIQPEAAEPKPTAEAP
KAPRVEEIPQRMTRNRAQMLANQSKQGPPPSEKECAPTPAPVTRAKARGSEDDDAQAQH
PRKRRFQRSTQQLQQQLNTSTQQTREVIQQTLAAIVDAIKLDAIEPYHSDRANPYFEYL
QIRKKIEEKRKILCCITPQAPQCYAEYVTYTGSYLLDGKPLSKLHIPVIAPPPSLAEPL
KELFRQQEAVRGKLRLQHSIEREKLIVSCEQEILRVHCRAARTIANQAVPFSACTMLLD
SEVYNMPLESQGDENKSVRDRFNARQFISWLQDVDDKYDRMKTCLLMRQQHEAAALNAV
QRMEWQLKVQELDPAGHKSLCVNEVPSFYVPMVDVNDDFVLLPA"
misc_feature 408..413
/label=SDS
misc_feature 1413..1418
/label=SDS
misc_feature 1539..1544
/label=SDS
misc_feature 1652..1657
/label=SDS
misc_feature 2200..2205
/label=SDS
misc_feature 2837..2842
/label=SDS
misc_feature 3182..3187
/label=SDS
misc_feature 3694..3699
/label=SDS
misc_feature 3789..3794
/label=SDS
misc_feature 4078..4083
/label=SDS
misc_feature 4200..4205
/label=SDS
CDS 4262..4327
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
polyA_signal 4331..4555
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 4578..4718
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4578..4707
/label=AAV2 ITR
repeat_region 4579..4719
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 4793..5248
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5530..5634
/gene="bla"
/label=AmpR promoter
CDS 5635..6495
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6666..7254
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtataact tcgtatagga tactttatag caatttatga
181 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
241 ctgacatcca ctttgccttt ctctccacag ggagagcgcc tgcctccctg agaagctgaa
301 agagaaggag aggcacagac actcctcatc ttcatccaag aagagccacg accgagagcg
361 agccaagaaa gagaaggccg agaagaaaga gaagggcgaa gattacaagg agggcggtag
421 caggaaggac tccggccagt acgaaaagga cttcctggag gcggatgctt acggagtttc
481 ttacaacatg aaagctgaca tagaagatga gctagataaa accattgaat tgttttctac
541 cgaaaagaaa gataaaaatg attccgagag agaaccttcc aagaaaatag aaaaggaact
601 aaagccttat ggatctagtg ccatcaacat cctaaaagag aagaagaaga gagagaaaca
661 cagggagaaa tggagagacg agaaggagag gcaccgggac aggcatgcgg atgggctgct
721 gcggcatcac agggacgagc tcctgcggca tcacagggac gagcagaagc ccgccaccag
781 ggacaaggac agcccgcccc gcgtgctcaa agacaagtcc agggacgagg gcccgaggct
841 cggcgatgcc aaactgaagg agaaattcaa ggacggtgca gagaaagaaa agggcgaccc
901 agtgaagatg agcaacggga atgataaggt agcgccatcc aaagacccag gcaagaaaga
961 cgccaggccc agggagaagc tcctggggga cggcgacctg atgatgacca gcttcgagag
1021 gatgctgtcc cagaaggacc tggagatcga ggagcgccac aagcggcaca aggagaggat
1081 gaagcaaatg gagaagctga ggcaccggtc cggagacccc aagctcaagg agaaggcgaa
1141 gccggcagac gacgggcgga agaagggtct ggacattcct gctaagaaac cgccggggct
1201 ggaccctcca tttaaagaca aaaagctcaa agagtcgact cctattccac ctgccgcgga
1261 aaataagcta cacccagcat caggtgcaga ctccaaagac tggctggcag gccctcacat
1321 gaaagaggtc ctgcctgcgt cccccaggcc tgaccagagc cggcccactg gcgtgcccac
1381 ccctacgtcg gtgctatcct gccccagcta cgaggaggtg atgcacacgc ccaggacccc
1441 gtcctgcagc gccgatgact acgcggacct cgtgttcgac tgcgccgact cgcagcactc
1501 cacgcccgtg cccaccgctc ccaccagcgc ctgctccccc tcctttttcg acaggttctc
1561 cgtggcttca agtgggcttt cggaaaacgc cagccaggct cctgccaggc ctctctccac
1621 aaacctttac cgctcggtct ctgtcgacat taggaggacc cccgaggaag aattcagcgt
1681 cggagacaag ctcttcaggc agcagagcgt tcctgctgcc tccagctacg actctcccat
1741 gccaccctcg atggaagaca gggcgcccct gcccccggtt cccgcggaga agtttgcctg
1801 cttgtcgcca gggtactact ccccagacta tggcctcccg tcgcccaaag tcgacgcttt
1861 gcactgccca ccggctgccg ttgtcactgt caccccgtct ccagagggcg tcttctcaag
1921 tttacaagca aaaccttccc cttcccccag agccgagctg ctggttcctt ccctcgaagg
1981 ggcccttccc ccggacctgg acacctccga ggaccagcag gcgacggccg ccatcatccc
2041 cccggagccc agctacctgg agccgctgga cgagggtccc ttcagcgccg tcatcaccga
2101 ggagcccgtt gagtgggccc acccctccga gcaggcgctt gcctctagcc tgatcggggg
2161 cacctctgaa aaccctgtga gctggcctgt gggctcggac ctcctgctga agtctccaca
2221 gagattcccc gagtccccaa agcgtttctg ccccgcggac cccctccact ctgccgcccc
2281 agggcccttc agcgcctcgg aggcgccgta ccccgcccct cccgcctctc ctgccccgta
2341 cgctctgccc gtcgctgagc cggggctgga ggacgtcaag gacggagtgg acgccgtccc
2401 cgccgccatc tccacctcag aggcggctcc ctacgcccct ccctccgggc tggagtcctt
2461 cttcagcaac tgcaagtcac ttccggaagc cccgctggac gtggcccccg agcccgcctg
2521 tgtagccgct gtggctcagg tggaggctct ggggcccctg gaaaatagct tcctggacgg
2581 cagccgcggc ctgtctcacc tcggccaggt ggagccggtg ccctgggcgg acgccttcgc
2641 cggccccgag gacgacctgg acctggggcc cttctccctg ccggagcttc ccctgcagac
2701 taaagatgcc gcagatggtg aagcggaacc cgtggaagaa agtcttgctc ctccagaaga
2761 gatgcctcca ggggcccccg gggtcataaa cggtggggat gtttccaccg tagtggctga
2821 ggagccgccg gcactgcctc ctgaccaggc ctccacccgg ctccctgcag agctcgagcc
2881 tgagccctca ggggagccaa agctggacgt ggctctagaa gctgcggtgg aggcggagac
2941 ggtgccggaa gagagggccc gtggggatcc ggactccagc gtggagcccg cgcccgttcc
3001 cccagaacag cgcccactgg ggagcggaga ccagggggct gaggctgaag gcccccccgc
3061 cgcgtccctc tgtgcccctg acggccccgc cccgaacact gtggcacaag ctcaggccgc
3121 agacggtgcc ggccccgagg acgacactga ggcctcccgt gccgccgccc cagccgaagg
3181 ccctcctggc ggcatccagc cggaagccgc agaaccaaaa cccacggccg aagccccgaa
3241 ggccccccga gtggaggaga tccctcagcg catgaccagg aaccgggcgc agatgctcgc
3301 gaaccagagc aagcagggcc cgcccccctc cgagaaggag tgcgccccca cccctgcccc
3361 ggtcaccagg gccaaggccc gcggctccga ggacgacgac gcccaggccc agcatccgcg
3421 caaacgccgc tttcagcgct ccacccagca gctgcagcag cagctgaaca cgtccacgca
3481 gcagacgcgg gaggtgatcc agcagacgct ggccgccatc gtggacgcca tcaagctgga
3541 tgccatcgag ccctaccaca gcgacagggc caacccctac ttcgaatacc tgcagatcag
3601 gaagaagatc gaggagaagc gcaagatcct gtgctgtatc acgccgcagg cgccccagtg
3661 ctacgccgag tacgtcacct acacgggctc ctacctcctg gacggcaagc cgctcagcaa
3721 gctccacatc cccgtgatcg caccccctcc ctccctggcg gagcccctga aggagctgtt
3781 caggcagcag gaggccgtcc ggggaaagct gcgtctacag cacagcatcg agcgggagaa
3841 gctgatcgta tcctgtgagc aggagattct gcgggttcac tgccgggcgg ccaggaccat
3901 cgccaaccag gcagtgccat tcagcgcctg cacgatgctg ctggactccg aggtctacaa
3961 catgcccctg gagagccagg gtgacgagaa caagtcagtg cgcgaccgtt tcaacgcccg
4021 ccagttcatc tcctggctcc aggacgtgga tgacaagtat gaccgcatga agacttgcct
4081 cctcatgcgg cagcagcacg aggccgcggc cctgaacgcc gtgcagagga tggagtggca
4141 gctgaaggtg caggaactgg accccgccgg gcacaagtcc ctgtgcgtga acgaggtgcc
4201 ctccttctac gtgcccatgg tcgacgtcaa cgacgacttt gtattgttgc cggcatgtac
4261 agactacaaa gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga
4321 tgacaagtaa ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
4381 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
4441 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
4501 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcacgt
4561 gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc
4621 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
4681 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct
4741 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc
4801 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
4861 gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
4921 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
4981 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
5041 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
5101 ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt
5161 ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
5221 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat
5281 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
5341 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
5401 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
5461 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
5521 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
5581 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
5641 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
5701 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
5761 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
5821 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
5881 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
5941 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
6001 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
6061 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
6121 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
6181 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
6241 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
6301 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
6361 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
6421 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
6481 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
6541 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
6601 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
6661 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
6721 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
6781 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
6841 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
6901 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
6961 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
7021 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
7081 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
7141 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
7201 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
7261 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//
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