pCytERM-mScarlet

LOCUS       pCytERM-mScarlet         4791 bp DNA     circular SYN 22-OCT-2024
DEFINITION  TSN20241013-0571-00010_743_20241014-BAN4_B02_B02_i20.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 22, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..4791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        61..165
                     /gene="bla"
                     /label=AmpR promoter
     promoter        167..524
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      375..510
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             559..1353
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             559..1353
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    1585..1632
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1961..2549
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     enhancer        2724..3027
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3028..3231
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             3283..3369
                     /codon_start=1
                     /label=cytochrome p450
                     /translation="MDPVVVLGLCLSCLLLLSLWKQSYGGGKL"
     regulatory      3415..3424
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3421..4119
                     /codon_start=1
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     polyA_signal    4242..4363
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(join(4370..4791,1..34))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg
       61 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
      121 caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga
      181 accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
      241 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
      301 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
      361 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
      421 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
      481 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca
      541 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
      601 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
      661 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
      721 ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc
      781 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
      841 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
      901 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
      961 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     1021 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     1081 aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     1141 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     1201 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     1261 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     1321 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     1381 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     1441 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     1501 tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa
     1561 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg
     1621 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
     1681 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
     1741 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct
     1801 caggttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     1861 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1921 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1981 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2041 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2101 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2161 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2221 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2281 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2341 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2401 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2461 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2521 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2581 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2641 ataaccgtat taccgccatg cattagttat taatagtaat caattacggg gtcattagtt
     2701 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     2761 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     2821 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     2881 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     2941 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     3001 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     3061 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     3121 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     3181 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg
     3241 aaccgtcaga tccgctagcg ctaccggact cagatctcca ccatggatcc tgtggtggtg
     3301 ctggggctct gtctctcctg tttgcttctc ctttcactct ggaaacagag ctatggggga
     3361 gggaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccacc ggtcgccacc
     3421 atggtgagca agggagaggc cgtgatcaag gagttcatga gattcaaggt gcacatggag
     3481 ggaagcatga acggacacga gttcgagatc gagggcgagg gcgagggaag gccatacgag
     3541 gggacccaga cagcaaagct gaaggtgaca aagggcggac ccctgccttt tagctgggac
     3601 atcctgagcc cacaatttat gtatggcagc agagccttta ccaagcaccc cgccgacatc
     3661 cccgactact acaaacaatc ctttcccgag ggctttaaat gggaacgggt gatgaacttc
     3721 gaagacggcg gcgccgtgac cgtgacccag gataccagcc tggaagacgg aaccctgatc
     3781 tacaaagtga agctcagagg caccaacttc ccccccgacg gccccgttat gcagaagaag
     3841 acaatgggtt gggaggccag caccgagaga ctctaccccg aggacggcgt gctcaagggc
     3901 gacattaaga tggcactgcg cctgaaggac ggcggaagat acctggccga cttcaagacc
     3961 acctacaagg ccaaaaagcc cgtgcagatg cccggcgcat acaacgtgga cagaaagctg
     4021 gacataacca gccacaacga agactacacc gtggtggagc aatacgagag aagcgaagga
     4081 aggcacagca caggaggcat ggatgagctg tataaataaa gcggccgcga ctctagatca
     4141 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     4201 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     4261 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     4321 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc
     4381 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     4441 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     4501 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     4561 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     4621 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     4681 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     4741 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac a
//