| 质粒名称: | pCSIIbsr-6013x |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 108654 |
| 存储实验室: | Michiyuki Matsuda |
|
载体骨架基本信息 |
|
| 载体名称: | pCSIIbsr |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 10000 bp |
| 完整质粒大小: | 13663 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达,Lentiviral |
| 筛选标记: | 杀螨素 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Raichu-EV |
| 物种来源: | S. cerevisiae (budding yeast), Synthetic; aequorea coerulescens |
| 插入大小: | 2300 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | XFP（插入件上的N端子） |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pCSIIbsr-6013x was a gift from Michiyuki Matsuda (Addgene plasmid # 108654 ; http://n2t.net/addgene:108654 ; RRID:Addgene_108654) |
| 参考文献部分: | A platform of BRET-FRET hybrid biosensors for optogenetics, chemical screening, and in vivo imaging. Komatsu N, Terai K, Imanishi A, Kamioka Y, Sumiyama K, Jin T, Okada Y, Nagai T, Matsuda M. Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.1038/s41598-018-27174-x. 10.1038/s41598-018-27174-x PubMed 29895862 |
买家导航
pCSIIbsr-6013x载体 pCSIIbsr6013x质粒基本信息
pCSIIbsr-6013x质粒图谱 pCSIIbsr6013x载体图谱和pCSIIbsr-6013x载体序列 pCSIIbsr6013x质粒序列多克隆位点信息
pCSIIbsr-6013x质粒 pCSIIbsr6013x载体简介
pCSIIbsr-6013x质粒序列 pCSIIbsr6013x载体序列
LOCUS pCSIIbsr-6013x 13660 bp ds-DNA circular SYN 10-AUG-2018
DEFINITION An HRas biosensor based on fluorescence resonance energy transfer
(FRET) and those based on bioluminescence energy transfer (BRET).
ACCESSION .
VERSION .
KEYWORDS pCSIIbsr-6013x
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13660)
AUTHORS Komatsu N, Terai K, Imanishi A, Kamioka Y, Sumiyama K, Jin T, Okada
Y, Nagai T, Matsuda M
TITLE A platform of BRET-FRET hybrid biosensors for optogenetics, chemical
screening, and in vivo imaging.
JOURNAL Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.1038/s41598-018-27174-x.
PUBMED 29895862
REFERENCE 2 (bases 1 to 13660)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 10, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..13660
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 616..814
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 832..1012
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1059..1184
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1676..1909
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2412..2529
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2585..3747
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 2816..3738
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 3695..3715
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
CDS 3782..4492
/codon_start=1
/product="yellow fluorescent protein optimized for FRET
with CyPet (Nguyen and Daugherty, 2005)"
/label=YPet
/translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
LLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLE
FLTAAGITEGMNELY"
primer_bind complement(5678..5699)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(5939..5958)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
misc_feature 7326..7912
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(7493..7510)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 7720..7739
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 7913..8311
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
misc_feature 8355..8943
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(8408..8428)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(8826..8837)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 9462..9642
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(9668..9685)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 9674..9898
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 9944..10372
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(10031..10050)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 10241..10262
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(10381..10401)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 10386..10715
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 10566..10701
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 10628..10647
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 10763..10810
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 10829..11203
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 11333..11454
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(11370..11389)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 11424..11443
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(11503..11519)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(11503..11519)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(11516..11538)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 11527..11543
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(11551..11581)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 11596..11617
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(11734..11751)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(11905..12493)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(11985..12004)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(12664..13524)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 13287..13306
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(13525..13629)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
1501 gtaagaccac cgcacagcaa gggccgctga tcttcagacc tggaggagga gatatgaggg
1561 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
1621 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
1681 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
1741 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
1801 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
1861 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
1921 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
1981 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
2041 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
2101 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
2161 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
2221 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
2281 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
2341 gtggagagag agacagagac agatccattc gattagtgaa cggatctaca aatggcagta
2401 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
2461 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
2521 caaaattttc gggtttatta cagggacagc agaaattcac tttgaattaa ttcaagcttc
2581 gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
2641 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
2701 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
2761 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
2821 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
2881 aattacttcc acctggctcc agtacgtgat tcttgatccc gagctggagc caggggcggg
2941 ccttgcgctt taggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg
3001 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct
3061 ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt
3121 gtaaatgcgg gccaggatct gcacactggt atttcggttt ttggggccgc gggcggcgac
3181 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg
3241 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
3301 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
3361 gaaagatggc cgcttcccgg ccctgctcca gggggctcaa aatggaggac gcggcgctcg
3421 ggagagcggg cgggtgagtc acccacacaa aggaaagggg cctttccgtc ctcagccgtc
3481 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctggagc
3541 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
3601 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
3661 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
3721 ttttttcttc catttcaggt gtcgtgaaca cgctaccggt ctcgacgaat tcgcccttag
3781 catgtctaaa ggtgaagaat tattcactgg tgttgtccca attttggttg aattagatgg
3841 tgatgttaat ggtcacaaat tttctgtctc cggtgaaggt gaaggtgatg ctacgtacgg
3901 taaattgacc ttaaaattac tctgtactac tggtaaattg ccagttccat ggccaacctt
3961 agtcactact ttaggttatg gtgttcaatg ttttgctaga tacccagatc atatgaaaca
4021 acatgacttt ttcaagtctg ccatgccaga aggttatgtt caagaaagaa ctattttttt
4081 caaagatgac ggtaactaca agaccagagc tgaagtcaag tttgaaggtg ataccttagt
4141 taatagaatc gaattaaaag gtattgattt taaagaagat ggtaacattt taggtcacaa
4201 attggaatac aactataact ctcacaatgt ttacatcact gctgacaaac aaaagaatgg
4261 tatcaaagct aacttcaaaa ttagacacaa cattgaagat ggtggtgttc aattagctga
4321 ccattatcaa caaaatactc caattggtga tggtccagtc ttgttaccag acaaccatta
4381 cttatcctat caatctgcct tattcaaaga tccaaacgaa aagagagacc acatggtctt
4441 gttagaattt ttgactgctg ctggtattac cgagggtatg aatgaattgt acctcgagat
4501 gacggaatat aagctggtgg tggtgggcgc cggcggtgtg ggcaagagtg cgctgaccat
4561 ccagctgatc cagaaccatt ttgtggacga atacgacccc actatagagg attcctaccg
4621 gaagcaggtg gtcattgatg gggagacgtg cctgttggac atcctggata ccgccggcca
4681 ggaggagtac agcgccatgc gggaccagta catgcgcacc ggggagggct tcctgtgtgt
4741 gtttgccatc aacaacacca agtcttttga ggacatccac cagtacaggg agcagatcaa
4801 acgggtgaag gactcggatg acgtgcccat ggtgctggtg gggaacaagt gtgacctggc
4861 tgcacgcact gtggaatctc ggcaggctca ggacctcgcc cgaagctacg gcatccccta
4921 catcgagacc tcggccaaga cccggcaggg agtggaggat gccttctaca cgttggtgcg
4981 tgagatccgg cagcacaagc tgcggaagct gaacggctcc ggaggaacca gtgctggtgg
5041 tagtgctggt ggtagtgctg gtggtagtgc tggtggtagt gctggtggtt ccggcagtgc
5101 tggtggtagt gctggtggta gtaccagtgc tggtggtagt gctggtggta gtgctggtgg
5161 tagtgctggt ggtagtgctg gtggttccgg cagtgctggt ggtagtgctg gtggtagtac
5221 cagtgctggt ggtagtgctg gtggtagtgc tggtggtagt gctggtggta gtgctggtgg
5281 ttccggcagt gctggtggta gtgctggtgg tagtaccagt gctggtggta gtgctggtgg
5341 tagtgctggt ggtagtgctg gtggtagtgc tggtggtacc ccttctaaga caagcaacac
5401 tatccgtgtt ttcttgccga acaagcaaag aacagtggtc aatgtgcgaa atggaatgag
5461 cttgcatgac tgccttatga aagcactcaa ggtgaggggc ctgcaaccag agagctgtgc
5521 agtgttcaga cttctccacg aacacaaagg taaaaaagca cgcttagatt ggaatactga
5581 agctgcgtct ttgattggag aagaacttca cgtagatttc ctgggcggcc gcatggtgag
5641 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
5701 caacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
5761 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
5821 caccctgtcc tggggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
5881 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
5941 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
6001 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
6061 gtacaactac ttcagcggga acgtctatat caccgccgac aagcagaaga acggcatcaa
6121 ggccaacttc aagatccgcc acaacatcga ggacggcggc gtgcagctcg ccgaccacta
6181 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
6241 cacccagtcc gccttaagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
6301 gttcttgacc gccgccgggg gtaccaaggt gtacgacccc gagcagagga agaggatgat
6361 caccggcccc cagtggtggg ccaggtgcaa gcagatgaac gtgctggaca gcttcatcaa
6421 ctactacgac agcgagaagc acgccgagaa cgccgtgatc ttcctgcacg gcaacgccac
6481 tagcagctac ctgtggaggc acgtggtgcc ccacatcgag cccgtggcca ggtgcatcat
6541 ccccgatctg atcggcatgg gcaagagcgg caagagcggc aacggcagct acaggctgct
6601 ggaccactac aagtacctga ccgcctggtt cgagcttctg aacctgccca agaagatcat
6661 cttcgtgggc cacgactggg gcgccgccct ggccttccac tacgcctacg agcaccagga
6721 caggatcaag gccatcgtgc acatggagag cgtggtggac gtgatcgaga gctgggacga
6781 gtggccagac atcgaggagg acatcgccct gatcaagagc gaggagggcg agaagatggt
6841 gctggagaac aacttcttcg tggagaccgt gctgcccagc aagatcatga gaaagctgga
6901 gcccgaggag ttcgccgcct acctggagcc cttcaaggag aagggcgagg tgagaagacc
6961 caccctgagc tggcccagag agatccccct ggtgaagggc ggcaagcccg acgtggtgca
7021 gatcgtgaga aactacaacg cctacctgag agccagcgac gacctgccca agctgttcat
7081 cgagggcgac cccggcttct tcagcaacgc catcgtggag ggcgccaaga agttccccaa
7141 caccgagttc gtgaaggtga agggcctgca cttcctccag gaggacgccc ccgacgagat
7201 gggcaagtac atcaagagct tcgtggagag agtgctgaag aacgagcagt ctagaaagat
7261 gagcaaagat ggtaaaaaga agaaaaagaa gtcaaagaca aagtgtgtaa ttatgtaagg
7321 atccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
7381 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
7441 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
7501 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
7561 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
7621 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
7681 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
7741 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
7801 cggtacacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
7861 cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatggccaa
7921 gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat
7981 ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt
8041 cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg
8101 cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa
8161 caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg
8221 gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt
8281 gctgccctct ggttatgtgt gggagggcta agtcgagcct ctagagttaa catcgaggga
8341 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
8401 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
8461 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
8521 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
8581 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
8641 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
8701 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
8761 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
8821 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
8881 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
8941 cgcatcgata ccgtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag
9001 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag
9061 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
9121 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc
9181 ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag
9241 ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
9301 aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc
9361 tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag
9421 catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc
9481 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
9541 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
9601 gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc
9661 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
9721 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
9781 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
9841 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
9901 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
9961 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
10021 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
10081 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
10141 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
10201 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
10261 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
10321 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
10381 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
10441 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
10501 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
10561 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
10621 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
10681 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
10741 ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt
10801 ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct
10861 caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg
10921 ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag
10981 cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct
11041 ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg
11101 gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc
11161 ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt
11221 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
11281 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
11341 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
11401 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
11461 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
11521 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
11581 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
11641 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
11701 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
11761 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
11821 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
11881 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
11941 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
12001 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
12061 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
12121 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
12181 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
12241 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
12301 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
12361 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
12421 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
12481 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
12541 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
12601 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
12661 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
12721 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
12781 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
12841 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
12901 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
12961 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
13021 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
13081 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
13141 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
13201 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
13261 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
13321 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
13381 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
13441 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
13501 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
13561 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
13621 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
