| 质粒名称: | pAAVLINK-EF1a-HA-5'-DYSF |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001723 |
| 产品用途: | 5' AAVLINK plasmid for DYSF expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | DYSF |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-DYSF
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-DYSF载体质粒基本信息
pAAVLINK-EF1a-HA-5'-DYSF质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-DYSF载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-DYSF质粒载体简介
pAAVLINK-EF1a-HA-5'-DYSF质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-DYSF 6548 bp DNA circular SYN 20-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6548)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6548
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..3688
/codon_start=1
/label=DYSF
/translation="MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNP
VWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNA
PLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTED
QGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQ
IRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDE
PIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKT
SLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAV
MDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCE
KMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLP
TFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDL
PADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLAST
TQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVH
LALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQIT
EAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPA
HQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQF
AEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGD
WFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEK"
misc_feature 1213..1218
/label=SDS
CDS 1430..1441
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
misc_feature 1833..1838
/label=SDS
misc_feature 2513..2518
/label=SDS
intron 3689..3729
/label=Splicing Donor
misc_feature 3733..3766
/label=lox2272-JT1571
repeat_region 3811..3951
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3811..3940
/label=AAV2 ITR
rep_origin 4026..4481
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4763..4867
/gene="bla"
/label=AmpR promoter
CDS 4868..5728
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5899..6487
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat gctgtgctgc ctgctggtga gggccagcaa
781 cctccccagt gcgaagaagg accggcgcag cgaccctgtc gcaagcctga ctttccgagg
841 ggtgaagaag agaaccaaag tcatcaagaa cagcgtgaac cctgtatgga atgagggatt
901 tgaatgggac ctcaagggca tccccctgga ccagggctct gagcttcatg tggtggtcaa
961 agaccatgag acgatgggga ggaacaggtt cctgggggaa gccaaggtcc cactccgaga
1021 ggtcctcgcc acccctagtc tgtccgccag cttcaatgcc cccctgctgg acaccaagaa
1081 gcagcccaca ggggcctcgc tggtcctgca ggtgtcctac acaccgctgc ctggagctgt
1141 gcccctgttc ccgcccccta ctcctctgga gccctccccg actctgcctg acctggatgt
1201 agtggcagac acaggaggag aggaagacac agaggaccag ggactcactg gagatgaggc
1261 ggagccattc ctggatcaaa gcggaggccc gggggctccc accaccccaa ggaaactacc
1321 ttcacgtcct ccgccccact accccgggat caaaagaaag cgaagtgcgc ctacatctag
1381 aaagctgctg tcagacaaac cgcaggattt ccagatcagg gtccaggtga tcgaggggcg
1441 ccagctgccg ggggtgaaca tcaagcctgt ggtcaaggtt accgctgcag ggcagaccaa
1501 gcggacgcgg atccacaagg gaaacagccc actcttcaat gagactcttt tcttcaactt
1561 gtttgactct cctggggagc tgtttgatga gcccatcttt atcacggtgg tagactctcg
1621 ttctctcagg acagatgctc tcctcgggga gttccggatg gacgtgggca ccatttacag
1681 agagccccgg cacgcctatc tcaggaagtg gctgctgctc tcagaccctg atgacttctc
1741 tgctggggcc agaggctacc tgaaaacaag cctttgtgtg ctggggcctg gggacgaagc
1801 gcctctggag agaaaagacc cctctgaaga caaggaggac attgaaagca acctgctccg
1861 gcccacaggc gtagccctgc gaggagccca cttctgcctg aaggtcttcc gggccgagga
1921 cttgccgcag atggacgatg ccgtgatgga caacgtgaaa cagatctttg gcttcgagag
1981 taacaagaag aacttggtgg acccctttgt ggaggtcagc tttgcgggga aaatgctgtg
2041 cagcaagatc ttggagaaga cggccaaccc tcagtggaac cagaacatca cactgcctgc
2101 catgtttccc tccatgtgcg aaaaaatgag gattcgtatc atagactggg accgcctgac
2161 tcacaatgac atcgtggcta ccacctacct gagtatgtcg aaaatctctg cccctggagg
2221 agaaatagaa gaggagcctg caggtgctgt caagccttcg aaagcctcag acttggatga
2281 ctacctgggc ttcctcccca cttttgggcc ctgctacatc aacctctatg gcagtcccag
2341 agagttcaca ggcttcccag acccctacac agagctcaac acaggcaagg gggaaggtgt
2401 ggcttatcgt ggccggcttc tgctctccct ggagaccaag ctggtggagc acagtgaaca
2461 gaaggtggag gaccttcctg cggatgacat cctccgggtg gagaagtacc ttaggaggcg
2521 caagtactcc ctgtttgcgg ccttctactc agccaccatg ctgcaggatg tggatgatgc
2581 catccagttt gaggtcagca tcgggaacta cgggaacaag ttcgacatga cctgcctgcc
2641 gctggcctcc accactcagt acagccgtgc agtctttgac gggtgccact actactacct
2701 accctggggt aacgtgaaac ctgtggtggt gctgtcatcc tactgggagg acatcagcca
2761 tagaatcgag actcagaacc agctgcttgg gattgctgac cggctggaag ctggcctgga
2821 gcaggtccac ctggccctga aggcgcagtg ctccacggag gacgtggact cgctggtggc
2881 tcagctgacg gatgagctca tcgcaggctg cagccagcct ctgggtgaca tccatgagac
2941 accctctgcc acccacctgg accagtacct gtaccagctg cgcacccatc acctgagcca
3001 aatcactgag gctgccctgg ccctgaagct cggccacagt gagctccctg cagctctgga
3061 gcaggcggag gactggctcc tgcgtctgcg tgccctggca gaggagcccc agaacagcct
3121 gccggacatc gtcatctgga tgctgcaggg agacaagcgt gtggcatacc agcgggtgcc
3181 cgcccaccaa gtcctcttct cccggcgggg tgccaactac tgtggcaaga attgtgggaa
3241 gctacagaca atctttctga aatatccgat ggagaaggtg cctggcgccc ggatgccagt
3301 gcagatacgg gtcaagctgt ggtttgggct ctcagtggat gagaaggagt tcaaccagtt
3361 tgctgagggg aagctgtctg tctttgctga aacctatgag aacgagacta agttggccct
3421 tgttgggaac tggggcacaa cgggcctcac ctaccccaag ttttctgacg tcacgggcaa
3481 gatcaagcta cccaaggaca gcttccgccc ctcggccggc tggacctggg ctggagattg
3541 gttcgtgtgt ccggagaaga ctctgctcca tgacatggac gccggtcacc tgagcttcgt
3601 ggaagaggtg tttgagaacc agacccggct tcccggaggc cagtggatct acatgagtga
3661 caactacacc gatgtgaacg gggagaaggt aagtatcaag gttacaagac aggtttaagg
3721 agaccaataa gctattattc gtataggata ctttatacga agttattaag ttatggagac
3781 cggtgtggca cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc
3841 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
3901 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga
3961 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
4021 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
4081 gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct
4141 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
4201 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
4261 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
4321 tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga
4381 tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa
4441 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
4501 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
4561 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
4621 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
4681 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
4741 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
4801 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
4861 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
4921 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
4981 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
5041 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
5101 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
5161 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
5221 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
5281 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
5341 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
5401 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
5461 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
5521 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
5581 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
5641 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
5701 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
5761 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
5821 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
5881 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
5941 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
6001 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
6061 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
6121 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
6181 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
6241 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
6301 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
6361 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
6421 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
6481 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
6541 tcacatgt
//
