| 质粒名称: | pAAVLINK-FLNA-B |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001752 |
| 产品用途: | Body' AAVLINK plasmid for FLNA expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | Promoterless |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | FLNA |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-FLNA-B
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-FLNA-B载体质粒基本信息
pAAVLINK-FLNA-B质粒图谱载体图谱和pAAVLINK-FLNA-B载体序列质粒序列多克隆位点信息
pAAVLINK-FLNA-B质粒载体简介
pAAVLINK-FLNA-B质粒序列载体序列
LOCUS pAAVLINK-FLNA-B 6148 bp DNA circular SYN 21-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6148)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6148
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
protein_bind 145..178
/label=loxJTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 179..270
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 271..3304
/codon_start=1
/label=FLNA
/translation="GPVGVNVTYGGDPIPKSPFSVAVSPSLDLSKIKVSGLGEKVDVGK
DQEFTVKSKGAGGQGKVASKIVGPSGAAVPCKVEPGLGADNSVVRFLPREEGPYEVEVT
YDGVPVPGSPFPLEAVAPTKPSKVKAFGPGLQGGSAGSPARFTIDTKGAGTGGLGLTVE
GPCEAQLECLDNGDGTCSVSYVPTEPGDYNINILFADTHIPGSPFKAHVVPCFDASKVK
CSGPGLERATAGEVGQFQVDCSSAGSAELTIEICSEAGLPAEVYIQDHGDGTHTITYIP
LCPGAYTVTIKYGGQPVPNFPSKLQVEPAVDTSGVQCYGPGIEGQGVFREATTEFSVDA
RALTQTGGPHVKARVANPSGNLTETYVQDRGDGMYKVEYTPYEEGLHSVDVTYDGSPVP
SSPFQVPVTEGCDPSRVRVHGPGIQSGTTNKPNKFTVETRGAGTGGLGLAVEGPSEAKM
SCMDNKDGSCSVEYIPYEAGTYSLNVTYGGHQVPGSPFKVPVHDVTDASKVKCSGPGLS
PGMVRANLPQSFQVDTSKAGVAPLQVKVQGPKGLVEPVDVVDNADGTQTVNYVPSREGP
YSISVLYGDEEVPRSPFKVKVLPTHDASKVKASGPGLNTTGVPASLPVEFTIDAKDAGE
GLLAVQITDPEGKPKKTHIQDNHDGTYTVAYVPDVTGRYTILIKYGGDEIPFSPYRVRA
VPTGDASKCTVTVSIGGHGLGAGIGPTIQIGEETVITVDTKAAGKGKVTCTVCTPDGSE
VDVDVVENEDGTFDIFYTAPQPGKYVICVRFGGEHVPNSPFQVTALAGDQPSVQPPLRS
QQLAPQYTYAQGGQQTWAPERPLVGVNGLDVTSLRPFDLVIPFTIKKGEITGEVRMPSG
KVAQPTITDNKDGTVTVRYAPSEAGLHEMDIRYDNMHIPGSPLQFYVDYVNCGHVTAYG
PGLTHGVVNKPATFTVNTKDAGEGGLSLAIEGPSKAEISCTDNQDGTCSVSYLPVLPGD
YSILVKYNEQHVPGSPFTARVT"
misc_feature 1418..1423
/label=SDS
intron 3305..3345
/label=Splicing Donor
misc_feature 3349..3382
/label=lox2272-JT1571
repeat_region 3411..3551
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3411..3540
/label=AAV2 ITR
rep_origin 3626..4081
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4363..4467
/gene="bla"
/label=AmpR promoter
CDS 4468..5328
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5499..6087
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtataact tcgtatagca tacattatag caatttatga
181 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
241 ctgacatcca ctttgccttt ctctccacag ggtccagtag gcgtcaatgt cacttatgga
301 ggggatccca tccctaagag ccctttctca gtggcagtat ctccaagcct ggacctcagc
361 aagatcaagg tgtctggcct gggagagaag gtggacgttg gcaaagacca ggagttcaca
421 gtcaaatcaa agggtgctgg tggtcaaggc aaagtggcat ccaagattgt gggcccctcg
481 ggtgcagcgg tgccctgcaa ggtggagcca ggcctggggg ctgacaacag tgtggtgcgc
541 ttcctgcccc gtgaggaagg gccctatgag gtggaggtga cctatgacgg cgtgcccgtg
601 cctggcagcc cctttcctct ggaagctgtg gcccccacca agcctagcaa ggtgaaggcg
661 tttgggccgg ggctgcaggg aggcagtgcg ggctcccccg cccgcttcac catcgacacc
721 aagggcgccg gcacaggtgg cctgggcctg acggtggagg gcccctgtga ggcgcagctc
781 gagtgcttgg acaatgggga tggcacatgt tccgtgtcct acgtgcccac cgagcccggg
841 gactacaaca tcaacatcct cttcgctgac acccacatcc ctggctcccc attcaaggcc
901 cacgtggttc cctgctttga cgcatccaaa gtcaagtgct caggccccgg gctggagcgg
961 gccaccgctg gggaggtggg ccaattccaa gtggactgct cgagcgcggg cagcgcggag
1021 ctgaccattg agatctgctc ggaggcgggg cttccggccg aggtgtacat ccaggaccac
1081 ggtgatggca cgcacaccat tacctacatt cccctctgcc ccggggccta caccgtcacc
1141 atcaagtacg gcggccagcc cgtgcccaac ttccccagca agctgcaggt ggaacctgcg
1201 gtggacactt ccggtgtcca gtgctatggg cctggtattg agggccaggg tgtcttccgt
1261 gaggccacca ctgagttcag tgtggacgcc cgggctctga cacagaccgg agggccgcac
1321 gtcaaggccc gtgtggccaa cccctcaggc aacctgacgg agacctacgt tcaggaccgt
1381 ggcgatggca tgtacaaagt ggagtacacg ccttacgagg agggactgca ctccgtggac
1441 gtgacctatg acggcagtcc cgtgcccagc agccccttcc aggtgcccgt gaccgagggc
1501 tgcgacccct cccgggtgcg tgtccacggg ccaggcatcc aaagtggcac caccaacaag
1561 cccaacaagt tcactgtgga gaccagggga gctggcacgg gcggcctggg cctggctgta
1621 gagggcccct ccgaggccaa gatgtcctgc atggataaca aggacggcag ctgctcggtc
1681 gagtacatcc cttatgaggc tggcacctac agcctcaacg tcacctatgg tggccatcaa
1741 gtgccaggca gtcctttcaa ggtccctgtg catgatgtga cagatgcgtc caaggtcaag
1801 tgctctgggc ccggcctgag cccaggcatg gttcgtgcca acctccctca gtccttccag
1861 gtggacacaa gcaaggctgg tgtggcccca ttgcaggtca aagtgcaagg gcccaaaggc
1921 ctggtggagc cagtggacgt ggtagacaac gctgatggca cccagaccgt caattatgtg
1981 cccagccgag aagggcccta cagcatctca gtactgtatg gagatgaaga ggtaccccgg
2041 agccccttca aggtcaaggt gctgcctact catgatgcca gcaaggtgaa ggccagtggc
2101 cccgggctca acaccactgg cgtgcctgcc agcctgcccg tggagttcac catcgatgca
2161 aaggacgccg gggagggcct gctggctgtc cagatcacgg atcccgaagg caagccgaag
2221 aagacacaca tccaagacaa ccatgacggc acgtatacag tggcctacgt gccagacgtg
2281 acaggtcgct acaccatcct catcaagtac ggtggtgacg agatcccctt ctccccgtac
2341 cgcgtgcgtg ccgtgcccac cggggacgcc agcaagtgca ctgtcacagt gtcaatcgga
2401 ggtcacgggc taggtgctgg catcggcccc accattcaga ttggggagga gacggtgatc
2461 actgtggaca ctaaggcggc aggcaaaggc aaagtgacgt gcaccgtgtg cacgcctgat
2521 ggctcagagg tggatgtgga cgtggtggag aatgaggacg gcactttcga catcttctac
2581 acggcccccc agccgggcaa atacgtcatc tgtgtgcgct ttggtggcga gcacgtgccc
2641 aacagcccct tccaagtgac ggctctggct ggggaccagc cctcggtgca gccccctcta
2701 cggtctcagc agctggcccc acagtacacc tacgcccagg gcggccagca gacttgggcc
2761 ccggagaggc ccctggtggg tgtcaatggg ctggatgtga ccagcctgag gccctttgac
2821 cttgtcatcc ccttcaccat caagaagggc gagatcacag gggaggttcg gatgccctca
2881 ggcaaggtgg cgcagcccac catcactgac aacaaagacg gcaccgtgac cgtgcggtat
2941 gcacccagcg aggctggcct gcacgagatg gacatccgct atgacaacat gcacatccca
3001 ggaagcccct tgcagttcta tgtggattac gtcaactgtg gccatgtcac tgcctatggg
3061 cctggcctca cccatggagt agtgaacaag cctgccacct tcaccgtcaa caccaaggat
3121 gcaggagagg ggggcctgtc tctggccatt gagggcccgt ccaaagcaga aatcagctgc
3181 actgacaacc aggatgggac atgcagcgtg tcctacctgc ctgtgctgcc gggggactac
3241 agcattctag tcaagtacaa tgaacagcac gtcccaggca gccccttcac tgctcgggtc
3301 acaggtaagt atcaaggtta caagacaggt ttaaggagac caataagcta ttattcgtat
3361 aggatacttt atacgaagtt atgaattcca cgtgcggacc gagcggccgc aggaacccct
3421 agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc
3481 aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag
3541 ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
3601 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
3661 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc
3721 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
3781 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
3841 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
3901 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct
3961 atctcgggct attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa
4021 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
4081 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
4141 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
4201 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
4261 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
4321 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
4381 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
4441 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
4501 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
4561 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
4621 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
4681 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
4741 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
4801 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
4861 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
4921 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
4981 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
5041 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
5101 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
5161 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
5221 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
5281 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
5341 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
5401 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
5461 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
5521 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
5581 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
5641 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
5701 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
5761 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
5821 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
5881 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
5941 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
6001 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
6061 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
6121 gccttttgct ggccttttgc tcacatgt
//
