| 质粒名称: | pAAVLINK-SRCAP-B |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001937 |
| 产品用途: | Body AAVLINK plasmid for SRCAP expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | Promoterless |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | SRCAP |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-SRCAP-B
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-SRCAP-B载体质粒基本信息
pAAVLINK-SRCAP-B质粒图谱载体图谱和pAAVLINK-SRCAP-B载体序列质粒序列多克隆位点信息
pAAVLINK-SRCAP-B质粒载体简介
pAAVLINK-SRCAP-B质粒序列载体序列
LOCUS pAAVLINK-SRCAP-B 7327 bp DNA circular SYN 10-AUG-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7327)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 16, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7327
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
protein_bind complement(145..178)
/label=loxJTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
intron 179..270
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 271..4483
/codon_start=1
/gene="SRCAP"
/gene_synonym="DEHMBA; DOMO1; EAF1; FLHS; SWR1"
/product="helicase SRCAP"
/label=SRCAP
/note="Snf2-related CBP activator protein;
Swi2/Snf2-related ATPase homolog, domino homolog 1; domino
homolog 2"
/db_xref="CCDS:CCDS10689.2"
/db_xref="GeneID:10847"
/db_xref="HGNC:HGNC:16974"
/db_xref="MIM:611421"
/protein_id="NP_006653.2"
/translation="PELSAQPTPGPVPQVLPASLMVSASPAGPPLIPASRPPGPVLLPP
LQPNSGSLPQVLPSPLGVLSGTSRPPTPTLSLKPTPPAPVRLSPAPPPGSSSLLKPLTV
PPGYTFPPAAATTTSTTTATATTTAVPAPTPAPQRLILSPDMQARLPSGEVVSIGQLAS
LAQRPVANAGGSKPLTFQIQGNKLTLTGAQVRQLAVGQPRPLQRNVVHLVSAGGQHHLI
SQPAHVALIQAVAPTPGPTPVSVLPSSTPSTTPAPTGLSLPLAANQVPPTMVNNTGVVK
IVVRQAPRDGLTPVPPLAPAPRPPSSGLPAVLNPRPTLTPGRLPTPTLGTARAPMPTPT
LVRPLLKLVHSPSPEVSASAPGAAPLTISSPLHVPSSLPGPASSPMPIPNSSPLASPVS
STVSVPLSSSLPISVPTTLPAPASAPLTIPISAPLTVSASGPALLTSVTPPLAPVVPAA
PGPPSLAPSGASPSASALTLGLATAPSLSSSQTPGHPLLLAPTSSHVPGLNSTVAPACS
PVLVPASALASPFPSAPNPAPAQASLLAPASSASQALATPLAPMAAPQTAILAPSPAPP
LAPLPVLAPSPGAAPVLASSQTPVPVMAPSSTPGTSLASASPVPAPTPVLAPSSTQTML
PAPVPSPLPSPASTQTLALAPALAPTLGGSSPSQTLSLGTGNPQGPFPTQTLSLTPASS
LVPTPAQTLSLAPGPPLGPTQTLSLAPAPPLAPASPVGPAPAHTLTLAPASSSASLLAP
ASVQTLTLSPAPVPTLGPAAAQTLALAPASTQSPASQASSLVVSASGAAPLPVTMVSRL
PVSKDEPDTLTLRSGPPSPPSTATSFGGPRPRRQPPPPPRSPFYLDSLEEKRKRQRSER
LERIFQLSEAHGALAPVYGTEVLDFCTLPQPVASPIGPRSPGPSHPTFWTYTEAAHRAV
LFPQQRLDQLSEIIERFIFVMPPVEAPPPSLHACHPPPWLAPRQAAFQEQLASELWPRA
RPLHRIVCNMRTQFPDLRLIQYDCGKLQTLAVLLRQLKAEGHRVLIFTQMTRMLDVLEQ
FLTYHGHLYLRLDGSTRVEQRQALMERFNADKRIFCFILSTRSGGVGVNLTGADTVVFY
DSDWNPTMDAQAQDRCHRIGQTRDVHIYRLISERTVEENILKKANQKRMLGDMAIEGGN
FTTAYFKQQTIRELFDMPLEEPSSSSVPSAPEEEEETVASKQTHILEQALCRAEDEEDI
RAATQAKAEQVAELAEFNENDGFPAGEGEEAGRPGAEDEEMSRAEQEIAALVEQLTPIE
RYAMKFLEASLEEVSREELKQAEEQVEAARKDLDQAKEEVFRLPQEEEEGPGAGDESSC
GTGGGTHRRSKKAKAPERPGTRVSERLRGARAETQGANHTPVISAHQTRSTTTPPRCSP
A"
misc_feature 381..386
/label=SDS
misc_feature 603..608
/label=SDS
misc_feature 1657..1662
/label=SDS
misc_feature 1995..2000
/label=SDS
misc_feature 3126..3131
/label=SDS
misc_feature 3171..3176
/label=SDS
misc_feature 3868..3873
/label=SDS
misc_feature 4159..4164
/label=SDS
misc_feature 4234..4239
/label=SDS
misc_feature 4237..4242
/label=SDS
misc_feature 4261..4266
/label=SDS
misc_feature 4264..4269
/label=SDS
misc_feature 4267..4272
/label=SDS
intron 4484..4524
/label=Splicing Donor
misc_feature 4528..4561
/label=lox2272-JT1571
repeat_region 4590..4730
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4590..4719
/label=AAV2 ITR
repeat_region 4591..4731
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 4805..5260
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5542..5646
/gene="bla"
/label=AmpR promoter
CDS 5647..6507
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6678..7266
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtataact tcgtatagca tacattatag caatttatga
181 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
241 ctgacatcca ctttgccttt ctctccacag gtcctgagct ctcagcccag cccacccctg
301 gcccagtccc ccaagtgctg ccagcatcac tgatggtttc agcctcacct gccgggcccc
361 cgcttattcc tgcatctcgg cctcctggcc ctgtcctctt gcctccactg cagcccaaca
421 gtggttctct cccccaggtg ttgccatccc ccctgggggt cctgagtggg acctcacggc
481 ctcccacgcc aaccttgtcc ctaaagccaa caccacctgc cccagttcgc ctgagcccag
541 ccccacctcc aggctcctct agcctgttga agcccctgac agtgccacca ggctacacct
601 tccctcctgc tgctgccacc accacttcta ccaccacggc aactgctacc accacagcag
661 tgccagctcc gactcctgca ccacagcgcc tcattctatc tcccgatatg caggctcgcc
721 tgccctcagg cgaagtggtc agcatcgggc agttagcctc actggcacaa cgtccagtgg
781 ctaatgcagg gggaagcaaa cctctcacct tccaaatcca gggcaacaag ctgactttga
841 ctggtgccca ggtgcgccag cttgctgtgg ggcagccccg cccgctgcaa aggaatgtgg
901 tgcacctcgt gtcagcaggg gggcagcacc atctcatcag ccagcctgcc catgtggccc
961 tcatccaggc cgtggccccg acccctggcc ctacccctgt ctctgtgctg ccttcttcga
1021 cccccagcac cacccctgcc cctactggcc tcagccttcc gcttgctgct aaccaggtgc
1081 caccaaccat ggtgaataat acaggcgtgg tgaagattgt agtgagacaa gcccctcggg
1141 atggactgac tcctgttcct ccattggccc cagcaccccg gcctccgagc tctgggcttc
1201 cagctgtgtt gaatccacgc cccacgttaa cccctggccg gctacccaca cctactctgg
1261 gtactgctcg agcccccatg cccacaccca ctctggtgag gcctcttctc aagctggtcc
1321 acagtccttc acctgaagtc agtgcttcag cccccggagc tgcccccttg accatctctt
1381 ctcctctcca cgtgccatcc tccctccctg ggccagcctc ttctccaatg ccaattccca
1441 actcctctcc ccttgctagt cctgtgtcct ctacagtctc agttccattg tcatcttcac
1501 tccccatctc tgtccccacc acacttcctg ccccagcctc ggctccactc accatcccca
1561 tctcagcccc cttgactgtt tctgcttcgg gcccagctct gttgaccagt gtgactccac
1621 cattggcacc tgttgtccca gcggctcctg gacctccctc cttggcacca tctggtgctt
1681 ccccgtcagc atcagccttg actctaggtt tggccacagc tccatccctg tcttcatctc
1741 agacacctgg tcaccctctg ttgttggctc ccacctcttc acatgttcca gggttgaact
1801 caaccgtggc cccagcatgc tcacctgtcc tggtgccagc ttcggctctg gccagtcctt
1861 ttccgtcagc accaaatcca gctccagctc aggcttccct tctggctcca gcatcttctg
1921 catctcaggc tctagccacc cctctggctc ctatggcggc tccacagaca gcaattctgg
1981 ctccttctcc agctcctcct ctggctcctc ttccggtcct ggcaccatcg ccaggtgctg
2041 ctcctgtcct ggcttcatca cagactccgg ttccagttat ggctccatcg tctactccag
2101 gaacctcttt agcctcagct tcaccggtac cagctccaac ccctgtgttg gctccatcat
2161 caactcaaac tatgctacca gccccggttc cgtcacctct cccgagcccg gcttctacgc
2221 agacactggc cctagcccca gctttagcac ccactcttgg aggctcatct ccatctcaga
2281 cactctcttt gggaacgggg aacccccagg gaccctttcc aactcagaca ttgtcattaa
2341 ctccagcatc atccctggta ccaactccag cccagacact gtctttggca ccaggaccac
2401 cactgggtcc aactcagacg ctgtctctgg ctccagcacc ccctctggct ccagcttctc
2461 cagtgggccc agccccagct cacacgctga ctttggctcc agcatcgtca tctgcttcac
2521 tcctggcccc agcttcagtg cagacactga ccttgagccc tgccccagtt cctaccctgg
2581 gcccggccgc agctcagacc ttggcgctgg ccccagcctc cacacagtcc ccagcttccc
2641 aggcatcttc ccttgtggtt tcggcatctg gtgccgctcc cttgcctgtc accatggtat
2701 cccggctgcc tgtttccaag gatgagcctg acacactgac attgcgctct ggtcccccca
2761 gccctccctc cactgctacc tcgtttggtg gcccccggcc tcgacgccag ccccccccac
2821 cacctcgttc ccctttttat ctggactccc tggaggaaaa gcggaagcgg cagcggtctg
2881 aacgcctgga acggattttc caacttagtg aggctcatgg ggccctggca cctgtgtatg
2941 ggactgaagt cctggatttc tgtaccctgc cccaacctgt tgccagcccc atcggccctc
3001 gttctcctgg ccccagccac cccacctttt ggacttatac cgaggctgcc caccgggctg
3061 tactgtttcc ccagcagcga ctagaccagc tgtcagaaat cattgagagg ttcatctttg
3121 tcatgcctcc tgtggaggca cctccccctt ccctgcatgc ctgccaccca cctccttggc
3181 tggccccacg tcaggcagcc ttccaggagc aattggcctc tgagctctgg ccccgggctc
3241 gtcctttgca ccgtattgtg tgtaacatgc gcacccagtt ccctgactta agactcatcc
3301 agtatgattg cggaaagttg cagacgttgg cagtgctgtt gcggcagctc aaggcagagg
3361 gccaccgagt gctcatcttc acccagatga cccgaatgct ggatgtattg gagcagtttc
3421 tcacctacca tggccatctc tacctgcgcc tggatggatc tactagagtt gaacagagac
3481 aggccttgat ggaacggttc aatgcagaca aacgcatatt ctgcttcatc ctttcaactc
3541 ggagtggggg tgtgggcgtg aacctgacag gagcagacac tgttgttttt tatgacagcg
3601 actggaatcc caccatggat gctcaggccc aggaccgctg tcaccgaatt ggccagaccc
3661 gggatgtcca catatatagg cttatcagtg aacggacagt ggaggagaac atcctaaaaa
3721 aggcaaatca gaagagaatg ttgggggaca tggccattga gggaggcaac ttcaccacag
3781 cctatttcaa acagcagacc atccgagagc tgtttgatat gcccctggag gaaccttcta
3841 gctcatccgt gccctctgcc cctgaagagg aggaagagac tgtggccagc aagcagactc
3901 atattctgga gcaggcattg tgtcgggcag aagatgaaga ggatatccgt gcagccaccc
3961 aggccaaggc tgaacaggtg gctgagcttg cagaatttaa tgagaacgat gggtttcctg
4021 ctggtgaggg agaggaagct ggccggcctg gggctgagga tgaggagatg tcccgggctg
4081 agcaggaaat tgctgccctc gtagaacagc tgacccccat tgagcgctat gccatgaaat
4141 tcctggaggc ctcactggag gaggtgagcc gagaggagct caaacaggca gaagagcaag
4201 tggaagctgc ccgcaaagac ctggaccaag ccaaggagga ggtgttccgc ctaccccaag
4261 aggaggagga ggggccgggg gctggggatg agagttcctg tgggactggt ggaggcaccc
4321 accggcgcag taaaaaggcc aaagcccctg agaggccggg gactcgtgtc agtgagcgtc
4381 ttcgtggagc ccgggctgag actcaagggg caaaccacac tcctgtcata tccgcccatc
4441 aaactcgcag caccaccaca ccaccccgct gcagtcctgc caggtaagta tcaaggttac
4501 aagacaggtt taaggagacc aataagctat tattcgtata ggatacttta tacgaagtta
4561 tgaattccac gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc
4621 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
4681 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
4741 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
4801 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
4861 accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc
4921 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
4981 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
5041 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
5101 agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat
5161 ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa
5221 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
5281 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
5341 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
5401 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
5461 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
5521 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
5581 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
5641 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
5701 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
5761 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
5821 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
5881 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
5941 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
6001 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
6061 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
6121 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
6181 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
6241 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
6301 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
6361 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
6421 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
6481 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
6541 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
6601 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
6661 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
6721 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
6781 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
6841 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
6901 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
6961 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
7021 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
7081 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
7141 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
7201 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
7261 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
7321 cacatgt
//
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