| 质粒名称: | pHes7-Achilles-Hes7 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 153528 |
| 存储实验室: | Ryoichiro Kageyama |
|
载体骨架基本信息 |
|
| 载体名称: | pBluescript |
| 载体出品公司: | Clontech |
| 空载体大小: | 2974 bp |
| 完整质粒大小: | 11926bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Hes7、hes family bHLH transcription factor 7 |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 8952bp |
| 启动子: | mouse Hes7 promoter |
| 标签/融合蛋白: | Achilles (N terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | unknown (unknown if destroyed) |
| 3’克隆位点: | unknown (unknown if destroyed) |
| 5’测序引物 | unknown |
|
文献引用方法 |
|
| 材料和方法部分: | pHes7-Achilles-Hes7 was a gift from Ryoichiro Kageyama (Addgene plasmid # 153528 ; http://n2t.net/addgene:153528 ; RRID:Addgene_153528) |
| 参考文献部分: | Coupling delay controls synchronized oscillation in the segmentation clock. Yoshioka-Kobayashi K, Matsumiya M, Niino Y, Isomura A, Kori H, Miyawaki A, Kageyama R. Nature. 2020 Apr;580(7801):119-123. doi: 10.1038/s41586-019-1882-z. Epub 2020 Jan 8. 10.1038/s41586-019-1882-z PubMed 31915376 |
买家导航
pHes7-Achilles-Hes7载体质粒基本信息
pHes7-Achilles-Hes7质粒图谱载体图谱和pHes7-Achilles-Hes7载体序列质粒序列多克隆位点信息
pHes7-Achilles-Hes7质粒载体简介
pHes7-Achilles-Hes7质粒序列载体序列
如果没有序列信息，请删除本句话LOCUS pHes7-Achilles-Hes7 11926 bp DNA circular SYN 14-APR-2025
DEFINITION Expresses fusion protein of Achilles/YFP and mouse Hes7 from mouse
Hes7 promoter. The reporter shows oscillatory expression with 2-3
periodicity when expressed in mouse presomitic mesoderm..
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11926)
AUTHORS Yoshioka-Kobayashi K, Matsumiya M, Niino Y, Isomura A, Kori H,
Miyawaki A, Kageyama R
TITLE Coupling delay controls synchronized oscillation in the segmentation
clock.
JOURNAL Nature. 2020 Apr;580(7801):119-123. doi: 10.1038/s41586-019-1882-z.
Epub 2020 Jan 8.
PUBMED 31915376
REFERENCE 2 (bases 1 to 11926)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1038/s41586-019-1882-z"; journalName: "Nature"; date: "2020-04";
volume: "580"; issue: "7801"; pages: "119-123"
COMMENT Sequence Label: pHes7-Achilles-Hes7
FEATURES Location/Qualifiers
source 1..11926
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 1..17
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 6..28
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 25..41
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 25..41
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 60..80
/label=T3
/note="T3 promoter, forward primer"
promoter 62..80
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind 117..133
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 117..133
/label=pBluescriptSK
/note="For pBluescript vector"
primer_bind complement(167..183)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(168..184)
/label=pBluescriptKS
/note="For pBluescript vector"
regulatory 5568..5577
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS join(5574..5576,5577..5579,5580..6290)
/codon_start=1
/product="yellow fluorescent protein (YFP) with fast and
efficient maturation (Nagai et al., 2002)"
/label=Venus
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTL
KLICTTGKLPVPWPTLVTTLGYGLAVFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGSGFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSFLSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(5619..5640)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(5880..5899)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 6227..6248
/label=EGFP-C
/note="EGFP, forward primer"
CDS 7070..7081
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(9173..9192)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(9174..9192)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(9202..9219)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(9202..9218)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(9211..9233)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 9360..9815
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(9447..9466)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 9657..9678
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 9841..9945
/gene="bla"
/label=AmpR promoter
CDS join(9946..10014,10015..10806)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(10164..10183)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 10977..11565
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 11466..11485
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 11719..11736
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 11853..11874
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter join(11889..11894,11895..11912,11913..11919)
/label=lac promoter
/note="promoter for the E. coli lac operon"
ORIGIN
1 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
61 caattaaccc tcactaaagg gaacaaaagc tggagctcca ccgcggtggc ggccgctcta
121 gaactagtgg atcccccggg ctgcaggaat tcgatatcaa gcttatcgat accgtcgacc
181 tcgacgctag gactctaggc atcaccgcat ccagacaccc cattcttcta gagtgaggct
241 ttgcagaatc acagttctta ccctctgctc cctttctctg tcttttctcc atcataccta
301 ctgactgcca aataagagtt tatgtaatgc atatggactg tgtccccaca aaagcagaag
361 ccttttgttc agagctgtct ctgtggggcc tggaacagag caggtcggct aactatgtac
421 tgtggcaggc agcattcaac ttttgtagct gtggtcttta aagtttgtaa gtgctttcca
481 gtggagaccc gtcctcaaaa caaacaacaa caaggagaga aaggagggag gaagaggaag
541 aaagcaataa aaaaagaaaa gaaaaaagtg ttcctttcta ttttatctga gattccccca
601 gtttagtttt gaatcataga acaaaagacc gagaagttag gccacagtta ctttatgagc
661 ccagaacgct cccctgcttt gctgttttga ctgagagatt gagtgttctt gctttctaag
721 gatacagaaa cagctgtcta ggaacatccc cgattcagga cctagggcat cctaaggaga
781 ccactttccc tttgaagaac taaaggagaa gaagaaggag cttgaaagcc aggcctgggg
841 agctgccctg caggctaggg gaagttaaaa ggaccaccag ctgctcctag tcttcctttc
901 accttctgca tccctagagg cctacatgtg gccatgtgct gccctcctcc gaacttcagt
961 gcggcccacc atagagcaag cgtagggaga catagatgcc tgtatatgcc tgtactgtta
1021 gtatatagcc atagcatgtc ctctctggac ggaggcttgt gaccttcaga gaaatcctac
1081 tcataccctc aaacaaaggt accacacaca gccacatctt cacatctgtc agggcaagga
1141 gctggagtcc aatcacccag ttcattcagc tggtctccct gaagggtaac cttaggctgg
1201 agggcctgag aagcacatag tgtttatttc tattttttat ttttcaagac agggtttctc
1261 tttgtaacac tggctgtccc agaactcaga gatgctcctg cctctgcctt ccaagtcctg
1321 ggattaaaag tgtgtgctgc cactgcctaa tgtacataaa atgtaaaaga aaaaaaaact
1381 aggcataata agtaatattt agaacaaagc aaagaatgta atgatttcta tcttttaaaa
1441 attggctctg ggccacttcc gtggtacact ccggaaggta gagtcaggca gctgtctgag
1501 tttgaggcca gtctggtcta tagggcaagt tccaggacag ccaggagtac acagataaac
1561 cctgtctcaa aaaataaaaa taaaaataga agttgaccaa tagaggggga aactctgggt
1621 tcaaccctca atactaatgg aaaataattc tgacaaacta tcagtgaggt aaaacagcaa
1681 gaaaagcaag accctgtctc agaaatgggt acaacgatga ctgtgatgat aactgatagc
1741 cagtaagtta tcatcacact gacatgaaat gccagaaaag caacctaact cctggtagac
1801 ctccacaata ttctcagcag actgcctacc tactttgtat atttttcttg gtgtgtatgt
1861 gcaatttaca gagttctata ggccctatga acaccttgtt ggaactttcc ccagactgtt
1921 ttcgggggtc cctcatttgc attagcatca tggtgaatca gccttgcact tgaagttctt
1981 ctgctccccc caccccctgc gtcccaccgc cccttaaaag ctttcagcct actgggaaag
2041 cctattttct taaaccagta ggtttgtttt ttcacttttc aaaatgagcc tttctcatca
2101 aatggcctgc ctatagcact tctgaggccc gtggggataa ccaggagctg gggtggggct
2161 ggagatgggt gagttgatgt ctgatggggg tggggaatca gcttcagtag cagggaagac
2221 cccttttcca tcgagtggtc gggagatgga gggtatgatg ttgggaaaag tggggttctc
2281 taactgctac tctttcttac agaacaatgg ctcaaactag gatgccaact tgaaagtagt
2341 ctttgttgcc aagccaaggc tggtcttgaa ttctccactg cacttagact aacccccaag
2401 cttggaatag gctatgcctg acattatcta tctatctatc tatctatcta tctatctatc
2461 tatctatcta tctatcttat atttcatctt acacctgcca tccccaaatt ctctgttccg
2521 ggttctcttt ctctaccacc agcagatctc ttttctatag gatttgttcg ttttgcttgg
2581 actcatggct gaccccgggg agatattatc cccaccgtga agttgcctct gactacttcg
2641 accgaactag tacttcactg tcacgtcttt gcacttatcc ttacaaccat gtgaaattat
2701 cctattctgc cttcttggat gtttccggtc ttcacaccac catccccgtc aaaatggacg
2761 tttcaggggc gctctgagcc ggtgtaaccc gaaagcctaa caagagtggt cacaaagcag
2821 gcacacacag atttaggctg agtgggcaaa tgtaccagtt gggctcacag agatgccaag
2881 atgcggtttc cttttctcct agggccatgg cccctgcttg gaaccgagaa gggagagttg
2941 ttactgacac attccgagac caggctgaaa cgtttaggag acagcccaga gaggagaaga
3001 aggcttaaac cggaattgag actgtgagag cctgagaagg cagggaggag gggcacagaa
3061 ggtggcggag cgctccggag gggagcggaa agccagcgcg gggcgtcagg gcccccgggt
3121 cgctcacaag tggccccagc aggtgaccat gccgggtccc gcctttcccg ggcgcggcgc
3181 gtgcgggatc aaagctgccg gcaaggggcg ggccagggag ctgcagggaa ctgggggtgc
3241 gagcgtggga gcgcagggtt gccggaggag acagggacca ggaaagacga cgaggtagcc
3301 cggagaggcc cagagaggga gcagcagaag gatgtgagac tcagaagccg gaaagtacac
3361 tctagacccg gagagacccc agcagaagtc tccgagagac ctgaaaaaac tatcagagag
3421 atggagtcgc tggaagtgct cagggaccag caaaaacgga aaactggaat gcccaggact
3481 gaatatgggg aaaaggtgct tcagagaaca gaaaatgaaa gggagagaga ggaagaggga
3541 aagacctgga agacctggcc ctcccctgct gaggaaggag agaactcttg ggcagtgatc
3601 ccagctccgt gtcctttctc agggttgcgc ctcagggagt gtggacaaag atgtgtgtcg
3661 aggcagccag aactcccgag ggttaggggt agattgtgag gacctgcgga gtcagagaat
3721 aattttggaa cgtccaagcc ggccacagaa acaaaacact acatatcagt tttggttaaa
3781 atgttaaagc gcaagaataa tacttgcttg atgtgctgtc aatttaactt tttcctagtt
3841 gaggtagacg gaaaaaaaaa aaaagaaaaa agctagaact gtgcattggc cgggaatcga
3901 acccgggcct cccgcgtggc aggcgagaat tctaccactg aaccaccaat gcagataagg
3961 agggaggtca taatgagcct atgtgaactt ctcagaggca gatccaatcc tacttctagg
4021 tgtgggaaaa ggttgtagag aatcattgag gtgggtggag cgtcaggcca tttttttctc
4081 tccgttggat ccggccaccg ggccataaag tcattccata tggccagggg cggccccaca
4141 cccgggtgca aactgcctca ggccccgagc ctcacgtgca ggtgagaaaa actcaacccc
4201 aatcctggtt cccaaggagg ggggcggtgg ggcctgctgg ggccacacat ctgtgcttcc
4261 atttggctga agtaggggaa ggtgggatgt actgagggcc ctaaccccaa atgggtgcct
4321 cctttctcaa gtaccgggcc cctcctgctc tgcctttggc aaggcagcct cttgccagaa
4381 aaccctctca ggatgtggag ggcctctggg aaggggttcc actttgtctc tgactccaca
4441 gctatcttct ggctcggttt gtagccctgt ctttattatg tcctttcccc aggccttctt
4501 gtcgtgttaa atttcgattt taccttctca ggcacttcgc cctgaagtct ccaggtgttg
4561 acatatccat ccttctgaga ggtcatgctc tttgtttgag gtgggtgaag atcccatttt
4621 gcaaccaaac ctttcttgtc cctttagcaa atctcacgtc tccctgctcc cctacagtcc
4681 ctagaggcct ccagagcgcc caccaactgg tgcactctat gttttcctgc cagctgtact
4741 cctgaattta gttttaaacc aagttcttgt aaacggaaag gtatcgcttc tcggcctctt
4801 ggctaactaa gatcaagggt agtgaatgga aagatttctt cactcacgta tacttcaagg
4861 attttttttc cccctagaag tttcctttgt cctcccttag tccagccatc cttaggaaaa
4921 tctgttcccg gggactcaag tcacagtatc tatagagtca gtttggcggc agattctcct
4981 tattactacg tctgcaggga taagtactgg agttattgtg ggaattgagt agaagagaca
5041 gatacaacgg cgccgtcttt tcccacagcc agccagtaga atccggtaga tctgctctgc
5101 acctttaggg gttgggagaa gattataata ttcagcctgg aggcagaagc atgccaacat
5161 ttatttcagg gttggtgggg ctatagtgaa cctccaagaa atctactagt ctaggagacc
5221 cccaaatttc atgcctaagt gatttcttcc cagttcactt agactctctt cccctgataa
5281 tctcctccct ccagttgggt cccgccttat tatacagctt tatggtcctt aaaccagcct
5341 gtggcccctt cctcccctta cttcccacat ttggggctag ttcctcccct tcccctcccc
5401 cttcactccc tgcctttccg ggagcctcgt gccggggtcc ttgagctggg catctagggg
5461 ctgaaggggg cggggccggg ccctagccct cctaccctgc agcggcggga tataaggatc
5521 taggcagcac gcgctgagtc caccgaaggg tccggaggag cactcgagcc accatggtga
5581 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
5641 taaacggcca caagttcagc gtgcgcggcg agggcgaggg cgatgccacc atcggcaagc
5701 tgaccctgaa gctgatctgc accaccggca agctgcccgt gccctggccc accctcgtga
5761 ccaccctggg ctacggcctg gccgtcttcg cccgctaccc cgaccacatg aagcagcacg
5821 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
5881 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
5941 gcatcgagct gaagggctcc ggcttcaagg aggacggcaa catcctgggg cacaagctgg
6001 agtacaactt caacagccac aacgtctata tcaccgccga caagcagaag aacggcatca
6061 aggccaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact
6121 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
6181 gctaccagtc cttcctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
6241 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag gtcacccggg
6301 agcgagctga gaatagggac ggccccaagg tgagaggtgg gaagggaggg aaatagggtc
6361 cctgttcagg ccagaggact taaaggttct aactctgtgg actaggagtg aggatggaga
6421 cacaagagtg aaaagaccga aagaacctgt aaagtataaa gagggcaggg tcagagccca
6481 acttgcgcgg gctgcggaag cggaggtcta ggggtttgct ttattgtggg ctaaggcttg
6541 tggctaggaa agtgccagaa tttgggcatc gtggcctatt ctagggctcg ggaagctggg
6601 gcaggtggat tatcgcaagt tcgaggccac agagtgcacc cctaccgaaa aaacaaacaa
6661 acaaacaaaa accaaaccaa aataaaacaa acaaacaaaa ccccacgaag ctatactata
6721 acatgagata agctagaggc catagctggt ggaggagggt ggaaacaaaa atagaggaaa
6781 ggctatggac gctgtctgta agcgcctggg aacccacatc tgactcaaag agaagacctt
6841 tgagtcgttg gttccggagg tgcgaggcca ggtgagagat attaggagaa cgagtgaagg
6901 gtttgagtga gaagaggagg tgtggtcgca ctgtgtgtct gcctttcacc ttaggtctct
6961 atcgcgcggc tcactagcct gggggcaaag agtggcccta aagttcttca gtgatacact
7021 gagagggaga cgacacaaga tagagatata aagagaaaag aagagtgcca tagaggggag
7081 aagagaggag aaagctgaac acagaaaggg cagggagaag tgggcgagcc acggaaagcg
7141 acccaaggga ctgggaagta gcagggagtg agagggaaac gaatggacca gacccaagca
7201 gcctgggatt aggggaaagg ggcgcatggg gggttttccc agcggcagga tcggacgctt
7261 gcgcggcggc gctgagtctc ggtccggtcg gcccgctctt cctttcccgc tggtcacaga
7321 tgctgaagcc gttggtggag aagcggcgcc gggaccgcat caaccgcagc ctagaagagc
7381 tgaggctgct gctcctggag aggaccaggg accaggtcag ttccaccgct ggccccaggc
7441 ccctaagcat tctgtacctg tgtcccagag ctttctactg gggtggggac ataagctgga
7501 gtggtgactc tggacccaag cctgctcctt gaaatcccat cccagacccc ctctcgctct
7561 cagaacttgc tcttctccac cttgctggtc tgtaagtttc cagtctggct cggtaaactg
7621 agcttccagc ctgcgtcagt ttcccacttg gggttttcta aaattcctta tctacctccc
7681 acgcctgatc aatgggcttc agttctgcgg tggttttcac catccgggtt gctgtttcgt
7741 acacctctca tattgggaac tggttccact ccctcaggtt ggacaggctc atctcggggt
7801 gggacgggaa cttttcttgg ccatctccac attcggtcgc gagtcatggg ggcacccgca
7861 gctcccgtat ccgcccctcc cttcttcccc tagccacttt ccccctctct ccccacccct
7921 ttctgtctct gcgcccctcc ccccgtctgt agaacctccg gaacccgaag ctggagaaag
7981 cggagatact ggagttcgcc gtgggctact tgagggagcg aagccgggtg gagcccccgg
8041 gtacagcgcg gggggtgggg cagcggaggg aggctgggag ggtgggagcc cggaaggctc
8101 cggccggggg aggggcgggc tctgaggtgg cctgggactt gggggagcgg ccagctgtgc
8161 accatggcga ggttaataac acccgcgcgt gtctgtctct gtgtctccct cattgccctc
8221 ctctgcacgt ccatccggcc tcctgtctct gtctgtgcgc ccgtctgacc tcggtatctc
8281 tgcgccccgc cccttccctc aaccccgttg ggcccctgcc ctgcccgcct gtcctcccgc
8341 cggccgcacc aggggttccc cggtccccag gccaggacgc cgaggcgctc gccagctgct
8401 acttgtccgg cttccgcgag tgcctgcttc gcttggcggc ctttgcgcac gacgccagcc
8461 cggccgcacg ctcccagctt ttctccgcgc tgcacggcta ccggcgcccc aaaccgcccc
8521 ggcccgaggc cgtagatcca gggctcccag cgccgcgccc accgctggac cccgcttcac
8581 caatccttgg ccccgcgctg caccagcgcc ccccagtgca ccagggccct cctagtcccc
8641 gcctcgcttg gtccccatcc cactgctcct ctcgcgccgg ggattcgggc gcgccggcgc
8701 ccctcaccgg actgctgccg ccaccgccac cgccttacag acaagacggg gcgcccaagg
8761 ccccgccact cccaccgccc gcgttttgga gaccttggcc ctgagctttt gggggctggg
8821 gcggggattg ggggtggtgt agagactcca gtcagagggc aggagaggga tccgggcgag
8881 gagggcagcg gggcgcgcgg gctcagcgcg cgggtgagat gtggtctata ttagagtatc
8941 tatataaata tatattttcc catccctttt ccctgtccca cttccctgtg tgtagcattg
9001 tactctctct accctagtcc agtcccagtc ccttcccgag tccctagtgc atggaataaa
9061 atcgttatta aatcccggtg tgtcccctag ccaggggcct gcctttatat tggcgccacg
9121 cctacttgcc cataccgcgg ccgcgtcgag ggggggcccg gtacccaatt cgccctatag
9181 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
9241 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
9301 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
9361 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
9421 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
9481 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
9541 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
9601 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
9661 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
9721 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
9781 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
9841 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
9901 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
9961 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
10021 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
10081 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
10141 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
10201 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
10261 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
10321 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
10381 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
10441 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
10501 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
10561 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
10621 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
10681 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
10741 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
10801 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
10861 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
10921 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
10981 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
11041 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
11101 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
11161 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
11221 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
11281 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
11341 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
11401 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
11461 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
11521 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
11581 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
11641 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
11701 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
11761 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
11821 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
11881 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaa
//
