| 质粒名称: | pH2B-Electra2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000207 |
| 产品用途: | Expresses Electra2 fused to C-terminus of H2B |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳细胞表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | H2B, Electra2 |
| 物种来源: | Homo sapiens, Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Dual-expression system for blue fluorescent protein optimization
Papadaki S., Wang X., Wang Y., Zhang H., Jia S., Liu S., Yang M., Zhang D., Jia J., Köster R.W., Namikawa K., Piatkevich K.D.
Sci. Reports 2022 121 12, 1–16 (2022)
|
pH2B-Electra2
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pH2B-Electra2载体质粒基本信息
pH2B-Electra2质粒图谱载体图谱和pH2B-Electra2载体序列质粒序列多克隆位点信息
pH2B-Electra2质粒载体简介
pH2B-Electra2质粒序列载体序列
LOCUS pH2B-Electra2 5100 bp DNA circular SYN 29-MAY-2023
DEFINITION Localization: Nucleus/Histones, Excitation: 383, Emission: 448.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5100)
TITLE Davidson Blue Fluorescent Proteins
REFERENCE 2 (bases 1 to 5100)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 29, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5100
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 68..371
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 372..575
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 526..546
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS 664..1041
/codon_start=1
/gene="HIST1H2BJ"
/product="human histone H2B"
/label=H2B
/translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
GELAKHAVSEGTKAITKYTSAK"
primer_bind 1023..1077
/label=0126-pCICalypso-intoH2B-F
regulatory 1054..1063
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 1060..1773
/codon_start=1
/label=Electra2
/translation="MVSKGEELIEENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
RIKVIEGGPLPFAFDILATSFLFGSKTFIKYPADIPDFFEQSFPEGFTWERVTRYEDGG
VVTVTQDTSLEDGGLVYNVKVRGVNFHSKGPVMQKKTEGWEPFTEMMYPADGGLRGYTD
IALKVDGGGHLHANIVTTYRSKKTVGNIKMPGVHAVDYRLERIEESDNETYVVLREVAV
AKYSNLGGGMDELFK"
primer_bind complement(1750..1801)
/label=0126-pCICalypso6A4-intoH2BCyt-R
polyA_signal 1895..2016
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1932..1951)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1986..2005
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2023..2478)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2160..2181)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2372..2391
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2505..2609
/gene="bla"
/label=AmpR promoter
promoter 2611..2968
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2634..2654)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2819..2954
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2881..2900
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(2904..2915)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3003..3797
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3057..3076)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3667..3686
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(3985..4004)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4029..4076
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4405..4993
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4894..4913
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
601 agcgctaccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcc
661 accatgccag agccagcgaa gtctgctccc gccccgaaaa agggctccaa gaaggcggtg
721 actaaggcgc agaagaaagg cggcaagaag cgcaagcgca gccgcaagga gagctattcc
781 atctatgtgt acaaggttct gaagcaggtc caccctgaca ccggcatttc gtccaaggcc
841 atgggcatca tgaattcgtt tgtgaacgac attttcgagc gcatcgcagg tgaggcttcc
901 cgcctggcgc attacaacaa gcgctcgacc atcacctcca gggagatcca gacggccgtg
961 cgcctgctgc tgcctgggga gttggccaag cacgccgtgt ccgagggtac taaggccatc
1021 accaagtaca ccagcgctaa ggatccaccg gtcgccacca tggtgtctaa gggcgaagag
1081 ctgatcgagg aaaatatgcg tatgaaggtg gtcatggaag gttcggtcaa cggccaccaa
1141 ttcaaatgca caggtgaagg agaaggcaga ccgtacgagg gagtgcaaac catgaggatc
1201 aaagtcatcg agggaggacc cctgccattt gcctttgaca ttcttgccac gtcgttcctg
1261 ttcggcagca agacctttat caagtacccg gccgacatcc ctgatttctt cgaacagtcc
1321 tttcctgagg gctttacttg ggaaagagtt acgagatacg aagatggtgg agtagtcacc
1381 gtcacgcagg acaccagcct tgaggatggt gggctcgtct acaacgtcaa ggtcagaggg
1441 gtaaactttc actccaaggg tcccgtgatg cagaagaaga ccgagggttg ggagcctttc
1501 acagagatga tgtatccagc agatggtggt ctgagaggat ataccgacat cgcactgaaa
1561 gttgatggtg gtggccatct gcacgccaac atcgtgacaa cttacaggtc aaaaaagacc
1621 gtcgggaaca tcaagatgcc cggtgtccat gccgttgatt accgcctgga aaggatcgaa
1681 gagagtgaca atgaaaccta cgtagtgcta agagaagtgg cagttgccaa atacagcaac
1741 cttggtggtg gcatggacga gctgttcaag taagcggccg cgactctaga tcataatcag
1801 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
1861 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
1921 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
1981 tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata
2041 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
2101 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
2161 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
2221 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
2281 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
2341 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
2401 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
2461 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
2521 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
2581 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt
2641 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
2701 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
2761 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
2821 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
2881 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
2941 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc
3001 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
3061 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
3121 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
3181 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
3241 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
3301 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
3361 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
3421 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
3481 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
3541 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
3601 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
3661 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
3721 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
3781 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
3841 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
3901 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
3961 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
4021 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa
4081 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
4141 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
4201 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat
4261 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
4321 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
4381 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
4441 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
4501 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
4561 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
4621 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
4681 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
4741 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
4801 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
4861 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
4921 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
4981 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
5041 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
//
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