pGEX-6p-1-CSDE1

LOCUS       pGEX-6p-1-CSDE1                7294 bp DNA     circular SYN 08-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7294)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Apr 17, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        183..211
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    219..235
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             258..911
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             918..941
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     misc_feature    complement(939..977)
                     /label=Primer R for TEV
     primer_bind     complement(939..977)
                     /label=YZY-P793
     CDS             951..971
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     misc_feature    957..995
                     /label=Primer F
     CDS             978..3278
                     /codon_start=1
                     /label=CSDE1
                     /translation="MSFDPNLLHNNGHNGYPNGTSAALRETGVIEKLLTSYGFIQCSER
                     QARLFFHCSQYNGNLQDLKVGDDVEFEVSSDRRTGKPIAVKLVKIKQEILPEERMNGQE
                     VFYLTYTPEDVEGNVQLETGDKINFVIDNNKHTGAVSARNIMLLKKKQARCQGVVCAMK
                     EAFGFIERGDVVKEIFFHYSEFKGDLETLQPGDDVEFTIKDRNGKEVATDVRLLPQGTV
                     IFEDISIEHFEGTVTKVIPKVPSKNQNDPLPGRIKVDFVIPKELPFGDKDTKSKVTLLE
                     GDHVRFNISTDRRDKLERATNIEVLSNTFQFTNEAREMGVIAAMRDGFGFIKCVDRDVR
                     MFFHFSEILDGNQLHIADEVEFTVVPDMLSAQRNHAIRIKKLPKGTVSFHSHSDHRFLG
                     TVEKEATFSNPKTTSPNKGKEKEAEDGIIAYDDCGVKLTIAFQAKDVEGSTSPQIGDKV
                     EFSISDKQRPGQQVATCVRLLGRNSNSKRLLGYVATLKDNFGFIETANHDKEIFFHYSE
                     FSGDVDSLELGDMVEYSLSKGKGNKVSAEKVNKTHSVNGITEEADPTIYSGKVIRPLRS
                     VDPTQTEYQGMIEIVEEGDMKGEVYPFGIVGMANKGDCLQKGESVKFQLCVLGQNAQTM
                     AYNITPLRRATVECVKDQFGFINYEVGDSKKLFFHVKEVQDGIELQAGDEVEFSVILNQ
                     RTGKCSACNVWRVCEGPKAVAAPRPDRLVNRLKNITLDDASAPRLMVLRQPRGPDNSMG
                     FGAERKIRQAGVID"
     misc_feature    complement(3261..3299)
                     /label=Primer R
     misc_feature    3279..3317
                     /label=Primer F for TEV
     promoter        3597..3701
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3702..4562
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4733..5321
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        5565..5642
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             5643..6725
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     protein_bind    6738..6759
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6774..6804
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6812..6828
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6836..6852
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             6848..7294
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGI"
     primer_bind     complement(6868..6884)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
      301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
      361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
      421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
      481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
      541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
      601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
      661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
      721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
      781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
      841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
      901 atcctccaaa atcggatctg gaagttctgt tccaggggcc cctgggatcc gaaaacctgt
      961 atttccaggg cggtagtatg agctttgatc caaaccttct ccacaacaat ggacataatg
     1021 ggtaccctaa tggtacttca gcagcactgc gtgaaactgg ggttattgaa aaactgttaa
     1081 cctcttacgg atttattcag tgttcagaac gtcaagctag acttttcttc cactgttcac
     1141 agtataatgg caacctgcaa gacttaaaag taggagatga tgttgaattt gaagtatcat
     1201 cggaccgacg gactgggaaa cccattgctg ttaaactggt gaagataaaa caagaaatcc
     1261 tccctgaaga acgaatgaat ggacaagaag tgttttatct gacttacacc cctgaagatg
     1321 tcgaagggaa cgttcagctg gaaactggag ataaaataaa ctttgtaatt gataacaata
     1381 aacatactgg tgctgtaagt gctcgcaaca ttatgctgtt gaaaaagaaa caagcccgct
     1441 gtcagggagt agtttgtgcc atgaaggagg catttggctt tattgaaaga ggtgatgttg
     1501 taaaagagat attctttcac tatagtgaat ttaagggtga cttagaaacc ttacagcctg
     1561 gcgatgatgt ggaattcaca atcaaggaca gaaatggtaa agaagttgca acagatgtca
     1621 gactattgcc tcaaggaaca gtcatttttg aagatatcag cattgaacat tttgaaggaa
     1681 ctgtaaccaa agttatccca aaagtaccca gtaaaaacca gaatgaccca ttgccaggac
     1741 gcatcaaagt tgactttgtg atccctaaag aacttccctt tggagacaaa gatacgaaat
     1801 ccaaggtgac cctgctggaa ggtgaccatg ttaggtttaa tatttcaaca gaccgacgtg
     1861 acaaattaga gcgagcaacc aatatagaag ttctgtcaaa tacatttcag ttcactaatg
     1921 aagcccgaga aatgggtgtg attgctgcca tgagagatgg ttttggtttc atcaagtgtg
     1981 tggatcgtga tgttcgtatg ttcttccact tcagtgaaat tctggatggg aaccagctcc
     2041 atattgcaga tgaagtagag tttactgtgg ttcctgatat gctctctgct caaagaaatc
     2101 atgctattag gattaaaaaa cttcccaagg gcacggtttc atttcattcc cattcagatc
     2161 accgttttct gggcacggta gaaaaagaag ccactttttc caatcctaaa accactagcc
     2221 caaataaagg caaagagaag gaggctgagg atggcattat tgcttatgat gactgtgggg
     2281 tgaaactgac tattgctttt caagccaagg atgtggaagg atctacttct cctcaaatag
     2341 gagataaggt tgaatttagt attagtgaca aacagaggcc tggacagcag gttgcaactt
     2401 gtgtgcgact tttaggtcgt aattctaact ccaagaggct cttgggttat gtggcaactc
     2461 tgaaggataa ttttggattt attgaaacag ccaatcatga taaggaaatc tttttccatt
     2521 acagtgagtt ctctggtgat gttgatagcc tggaactggg ggacatggtc gagtatagct
     2581 tgtccaaagg caaaggcaac aaagtcagtg cagaaaaagt gaacaaaaca cactcagtga
     2641 atggcattac tgaggaagct gatcccacca tttactctgg caaagtaatt cgccccctga
     2701 ggagtgttga tccaacacag actgagtacc aaggaatgat tgagattgtg gaggagggcg
     2761 atatgaaagg tgaggtctat ccatttggca tcgttgggat ggccaacaaa ggggattgcc
     2821 tgcagaaagg ggagagcgtc aagttccaat tgtgtgtcct gggccaaaat gcacaaacta
     2881 tggcttacaa catcacaccc ctgcgcaggg ccacagtgga atgtgtgaaa gatcagtttg
     2941 gcttcattaa ctatgaagta ggagatagca agaagctctt tttccatgtg aaagaagttc
     3001 aggatggcat tgagctacag gcaggagatg aggtggagtt ctcagtgatt cttaatcagc
     3061 gcactggcaa gtgcagcgcc tgtaatgttt ggcgagtctg tgagggcccc aaggctgttg
     3121 cagctcctcg acctgatcgg ttggtcaatc gcttgaagaa tatcactctg gatgatgcca
     3181 gtgctcctcg cctaatggtt cttcgtcagc caaggggacc agataactca atggggtttg
     3241 gtgcagaaag aaagatccgt caagctggtg tcattgacct cgagcggccg catcgtgact
     3301 gactgacgat ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     3361 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     3421 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
     3481 gcggagtgta taattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
     3541 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     3601 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     3661 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     3721 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     3781 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     3841 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     3901 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
     3961 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     4021 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     4081 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     4141 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     4201 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa
     4261 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     4321 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     4381 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     4441 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     4501 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     4561 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     4621 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     4681 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     4741 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     4801 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     4861 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     4921 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     4981 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     5041 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     5101 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     5161 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     5221 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     5281 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     5341 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     5401 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     5461 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     5521 ttctccttac gcatctgtgc ggtatttcac accgcataaa ttccgacacc atcgaatggc
     5581 gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga
     5641 atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg
     5701 tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag
     5761 cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac
     5821 agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg
     5881 tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag
     5941 aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca
     6001 gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct
     6061 gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta
     6121 ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc
     6181 agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg
     6241 gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact
     6301 ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca
     6361 ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt
     6421 ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct
     6481 catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca
     6541 gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc
     6601 ccgtctcact ggtgaaaaga aaaaccacgc tagcgcccaa tacgcaaacc gcctctcccc
     6661 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     6721 agtgagcgca acgcaattaa tgtaagttag ctcactcatt aggcacccca ggctttacac
     6781 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     6841 aacagctatg accatgatta cggattcact ggccgtcgtt ttacaacgtc gtgactggga
     6901 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     6961 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     7021 atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga
     7081 tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc
     7141 gcccatctac accaacgtga cctatcccat tacggtcaat ccgccgtttg ttcccacgga
     7201 gaatccgacg ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg
     7261 ccagacgcga attatttttg atggcgttgg aatt
//