pAAVLINK-EF1a-HA-BRCA2-A

LOCUS       pAAVLINK-EF1a-HA-BRCA2-A            7246 bp DNA     circular SYN 24-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7246)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 8, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        146..688
                     /label=EF1-alpha
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
     promoter        175..386
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             399..667
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    493..498
                     /label=SDS
     CDS             704..730
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             737..4386
                     /codon_start=1
                     /label=BRCA2
                     /translation="MPIGSKERPTFFEIFKTRCNKADLGPISLNWFEELSSEAPPYNSE
                     PAEESEHKNNNYEPNLFKTPQRKPSYNQLASTPIIFKEQGLTLPLYQSPVKELDKFKLD
                     LGRNVPNSRHKSLRTVKTKMDQADDVSCPLLNSCLSESPVVLQCTHVTPQRDKSVVCGS
                     LFHTPKFVKGRQTPKHISESLGAEVDPDMSWSSSLATPPTLSSTVLIVRNEEASETVFP
                     HDTTANVKSYFSNHDESLKKNDRFIASVTDSENTNQREAASHGFGKTSGNSFKVNSCKD
                     HIGKSMPNVLEDEVYETVVDTSEEDSFSLCFSKCRTKNLQKVRTSKTRKKIFHEANADE
                     CEKSKNQVKEKYSFVSEVEPNDTDPLDSNVANQKPFESGSDKISKEVVPSLACEWSQLT
                     LSGLNGAQMEKIPLLHISSCDQNISEKDLLDTENKRKKDFLTSENSLPRISSLPKSEKP
                     LNEETVVNKRDEEQHLESHTDCILAVKQAISGTSPVASSFQGIKKSIFRIRESPKETFN
                     ASFSGHMTDPNFKKETEASESGLEIHTVCSQKEDSLCPNLIDNGSWPATTTQNSVALKN
                     AGLISTLKKKTNKFIYAIHDETSYKGKKIPKDQKSELINCSAQFEANAFEAPLTFANAD
                     SGLLHSSVKRSCSQNDSEEPTLSLTSSFGTILRKCSRNETCSNNTVISQDLDYKEAKCN
                     KEKLQLFITPEADSLSCLQEGQCENDPKSKKVSDIKEEVLAAACHPVQHSKVEYSDTDF
                     QSQKSLLYDHENASTLILTPTSKDVLSNLVMISRGKESYKMSDKLKGNNYESDVELTKN
                     IPMEKNQDVCALNENYKNVELLPPEKYMRVASPSRKVQFNQNTNLRVIQKNQEETTSIS
                     KITVNPDSEELFSDNENNFVFQVANERNNLALGNTKELHETDLTCVNEPIFKNSTMVLY
                     GDTGDKQATQVSIKKDLVYVLAEENKNSVKQHIKMTLGQDLKSDISLNIDKIPEKNNDY
                     MNKWAGLLGPISNHSFGGSFRTASNKEIKLSEHNIKKSKMFFKDIEEQYPTSLACVEIV
                     NTLALDNQKKLSKPQSINTVSAHLQSSVVVSDCKNSHITPQMLFSKQDFNSNHNLTPSQ
                     KAEITELSTILEESGSQFEFTQFRKPSYILQKSTFEVPENQMTILKTTSEECRDADLHV
                     IMNAPSIGQVDSSKQFEGTVEIKRKFAGLLKNDCNKSASGYLTDENEVGF"
     misc_feature    2382..2387
                     /label=SDS
     intron          4387..4427
                     /label=Splicing Donor
     misc_feature    4431..4464
                     /label=loxP-JT1571
     repeat_region   4509..4649
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4509..4638
                     /label=AAV2 ITR
     rep_origin      4724..5179
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5461..5565
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5566..6426
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6597..7185
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
      181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
      241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
      421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
      481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
      541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
      601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
      661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
      721 tgactatgct ggatctatgc ctattggatc caaagagagg ccaacatttt ttgaaatttt
      781 taagacacgc tgcaacaaag cagatttagg accaataagt cttaattggt ttgaagaact
      841 ttcttcagaa gctccaccct ataattctga acctgcagaa gaatctgaac ataaaaacaa
      901 caattacgaa ccaaacctat ttaaaactcc acaaaggaaa ccatcttata atcagctggc
      961 ttcaactcca ataatattca aagagcaagg gctgactctg ccgctgtacc aatctcctgt
     1021 aaaagaatta gataaattca aattagactt aggaaggaat gttcccaata gtagacataa
     1081 aagtcttcgc acagtgaaaa ctaaaatgga tcaagcagat gatgtttcct gtccacttct
     1141 aaattcttgt cttagtgaaa gtcctgttgt tctacaatgt acacatgtaa caccacaaag
     1201 agataagtca gtggtatgtg ggagtttgtt tcatacacca aagtttgtga agggtcgtca
     1261 gacaccaaaa catatttctg aaagtctagg agctgaggtg gatcctgata tgtcttggtc
     1321 aagttcttta gctacaccac ccacccttag ttctactgtg ctcatagtca gaaatgaaga
     1381 agcatctgaa actgtatttc ctcatgatac tactgctaat gtgaaaagct atttttccaa
     1441 tcatgatgaa agtctgaaga aaaatgatag atttatcgct tctgtgacag acagtgaaaa
     1501 cacaaatcaa agagaagctg caagtcatgg atttggaaaa acatcaggga attcatttaa
     1561 agtaaatagc tgcaaagacc acattggaaa gtcaatgcca aatgtcctag aagatgaagt
     1621 atatgaaaca gttgtagata cctctgaaga agatagtttt tcattatgtt tttctaaatg
     1681 tagaacaaaa aatctacaaa aagtaagaac tagcaagact aggaaaaaaa ttttccatga
     1741 agcaaacgct gatgaatgtg aaaaatctaa aaaccaagtg aaagaaaaat actcatttgt
     1801 atctgaagtg gaaccaaatg atactgatcc attagattca aatgtagcaa atcagaagcc
     1861 ctttgagagt ggaagtgaca aaatctccaa ggaagttgta ccgtctttgg cctgtgaatg
     1921 gtctcaacta accctttcag gtctaaatgg agcccagatg gagaaaatac ccctattgca
     1981 tatttcttca tgtgaccaaa atatttcaga aaaagaccta ttagacacag agaacaaaag
     2041 aaagaaagat tttcttactt cagagaattc tttgccacgt atttctagcc taccaaaatc
     2101 agagaagcca ttaaatgagg aaacagtggt aaataagaga gatgaagagc agcatcttga
     2161 atctcataca gactgcattc ttgcagtaaa gcaggcaata tctggaactt ctccagtggc
     2221 ttcttcattt cagggtatca aaaagtctat attcagaata agagaatcac ctaaagagac
     2281 tttcaatgca agtttttcag gtcatatgac tgatccaaac tttaaaaaag aaactgaagc
     2341 ctctgaaagt ggactggaaa tacatactgt ttgctcacag aaggaggact ccttatgtcc
     2401 aaatttaatt gataatggaa gctggccagc caccaccaca cagaattctg tagctttgaa
     2461 gaatgcaggt ttaatatcca ctttgaaaaa gaaaacaaat aagtttattt atgctataca
     2521 tgatgaaaca tcttataaag gaaaaaaaat accgaaagac caaaaatcag aactaattaa
     2581 ctgttcagcc cagtttgaag caaatgcttt tgaagcacca cttacatttg caaatgctga
     2641 ttcaggttta ttgcattctt ctgtgaaaag aagctgttca cagaatgatt ctgaagaacc
     2701 aactttgtcc ttaactagct cttttgggac aattctgagg aaatgttcta gaaatgaaac
     2761 atgttctaat aatacagtaa tctctcagga tcttgattat aaagaagcaa aatgtaataa
     2821 ggaaaaacta cagttattta ttaccccaga agctgattct ctgtcatgcc tgcaggaagg
     2881 acagtgtgaa aatgatccaa aaagcaaaaa agtttcagat ataaaagaag aggtcttggc
     2941 tgcagcatgt cacccagtac aacattcaaa agtggaatac agtgatactg actttcaatc
     3001 ccagaaaagt cttttatatg atcatgaaaa tgccagcact cttattttaa ctcctacttc
     3061 caaggatgtt ctgtcaaacc tagtcatgat ttctagaggc aaagaatcat acaaaatgtc
     3121 agacaagctt aaaggtaaca attatgaatc tgatgttgaa ttaaccaaaa atattcccat
     3181 ggaaaagaat caagatgtat gtgctttaaa tgaaaattat aaaaacgttg agctgttgcc
     3241 acctgaaaaa tacatgagag tagcatcacc ttcaagaaag gtacaattca accaaaacac
     3301 aaatctaaga gtaatccaaa aaaatcaaga agaaactact tcaatttcaa aaataactgt
     3361 caatccagac tctgaagaac ttttctcaga caatgagaat aattttgtct tccaagtagc
     3421 taatgaaagg aataatcttg ctttaggaaa tactaaggaa cttcatgaaa cagacttgac
     3481 ttgtgtaaac gaacccattt tcaagaactc taccatggtt ttatatggag acacaggtga
     3541 taaacaagca acccaagtgt caattaaaaa agatttggtt tatgttcttg cagaggagaa
     3601 caaaaatagt gtaaagcagc atataaaaat gactctaggt caagatttaa aatcggacat
     3661 ctccttgaat atagataaaa taccagaaaa aaataatgat tacatgaaca aatgggcagg
     3721 actcttaggt ccaatttcaa atcacagttt tggaggtagc ttcagaacag cttcaaataa
     3781 ggaaatcaag ctctctgaac ataacattaa gaagagcaaa atgttcttca aagatattga
     3841 agaacaatat cctactagtt tagcttgtgt tgaaattgta aataccttgg cattagataa
     3901 tcaaaagaaa ctgagcaagc ctcagtcaat taatactgta tctgcacatt tacagagtag
     3961 tgtagttgtt tctgattgta aaaatagtca tataacccct cagatgttat tttccaagca
     4021 ggattttaat tcaaaccata atttaacacc tagccaaaag gcagaaatta cagaactttc
     4081 tactatatta gaagaatcag gaagtcagtt tgaatttact cagtttagaa agccaagcta
     4141 catattgcag aagagtacat ttgaagtgcc tgaaaaccag atgactatct taaagaccac
     4201 ttctgaggaa tgcagagatg ctgatcttca tgtcataatg aatgccccat cgattggtca
     4261 ggtagacagc agcaagcaat ttgaaggtac agttgaaatt aaacggaagt ttgctggcct
     4321 gttgaaaaat gactgtaaca aaagtgcttc tggttattta acagatgaaa atgaagtggg
     4381 gtttaggtaa gtatcaaggt tacaagacag gtttaaggag accaataagc tattattcgt
     4441 atagcataca ttatacgaag ttattaagtt atggagaccg gtgtggcacg tgcggaccga
     4501 gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct
     4561 cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt
     4621 gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca
     4681 tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc
     4741 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     4801 ttagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     4861 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     4921 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     4981 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     5041 ggaacaacac tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt
     5101 tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     5161 atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     5221 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     5281 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     5341 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     5401 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     5461 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5521 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5581 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5641 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5701 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     5761 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     5821 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5881 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     5941 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     6001 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     6061 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     6121 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     6181 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     6241 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6301 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6361 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6421 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6481 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6541 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6601 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6661 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6721 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     6781 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6841 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6901 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6961 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7021 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7081 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7141 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7201 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgt
//