| 质粒名称: | pMaster12 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 58527 |
| 存储实验室: | Mario Capecchi |
|
载体骨架基本信息 |
|
| 载体名称: | pCEP4 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418),tk |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | 816 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pMaster12 was a gift from Mario Capecchi (Addgene plasmid # 58527 ; http://n2t.net/addgene:58527 ; RRID:Addgene_58527) |
| 参考文献部分: | Efficient germ-line transmission obtained with transgene-free induced pluripotent stem cells. Wu S, Wu Y, Zhang X, Capecchi MR. Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10678-83. doi: 10.1073/pnas.1409933111. Epub 2014 Jul 7. 10.1073/pnas.1409933111 PubMed 25002522 |
买家导航
pMaster12载体质粒基本信息
pMaster12质粒图谱载体图谱和pMaster12载体序列质粒序列多克隆位点信息
pMaster12质粒载体简介
pMaster12质粒序列载体序列
LOCUS pMaster12 27879 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Reprogram somatic cells to iPS cells.
ACCESSION .
VERSION .
KEYWORDS pMaster12
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 27879)
AUTHORS Wu S, Wu Y, Zhang X, Capecchi MR
TITLE Efficient germ-line transmission obtained with transgene-free
induced pluripotent stem cells.
JOURNAL Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10678-83. doi:
10.1073/pnas.1409933111. Epub 2014 Jul 7.
PUBMED 25002522
REFERENCE 2 (bases 1 to 27879)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..27879
/organism="synthetic DNA construct"
/mol_type="other DNA"
polyA_signal 87..134
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
promoter complement(1891..3069)
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 1900..2838
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
polyA_signal 3090..3137
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
CDS complement(3369..4172)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3480..3499)
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 4090..4109
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
misc_feature 4173..4759
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(4346..4365)
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
primer_bind 4575..4592
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
CDS complement(4775..5905)
/codon_start=1
/product="herpes simplex virus thymidine kinase"
/label=HSV TK
/note="confers sensitivity to nucleoside analogs such as
acyclovir or ganciclovir"
/translation="MASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQK
MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
PGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFILDYDQSPAGCRDAL
LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
intron 5978..6986
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
promoter complement(6987..7262)
/label=chicken beta-actin promoter
enhancer 7265..7568
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
rep_origin 7694..8149
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7781..7800)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7991..8012
/label=F1ori-F
/note="F1 origin, forward primer"
polyA_signal 8156..8277
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
CDS complement(8401..9720)
/codon_start=1
/product="human c-Myc proto-oncogene"
/label=c-Myc
/translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
CDS complement(9721..9780)
/codon_start=1
/product="2A peptide from equine rhinitis A virus
polyprotein"
/label=E2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="QCTNYALLKLAGDVESNPGP"
CDS complement(10741..10794)
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS complement(12214..12279)
/codon_start=1
/product="2A peptide from foot-and-mouth disease virus
polyprotein"
/label=F2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="VKQTLNFDLLKLAGDVESNPGP"
regulatory 13365..13374
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
promoter complement(13382..14560)
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 13391..14329
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
enhancer 14667..14970
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 14973..15248
/label=chicken beta-actin promoter
intron 15249..16257
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
polyA_signal 17251..17372
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin 17852..19641
/label=oriP
/note="Epstein-Barr virus oriP replication origin (Yates et
al., 2000)"
CDS complement(19943..21868)
/codon_start=1
/product="Epstein-Barr nuclear antigen 1, also known as
EBNA-1"
/label=EBNA1
/note="crucial for latent viral infection, and for episomal
amplification of vectors containing the oriP origin (Okita
et al., 2013)"
/translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
GEEGQE"
primer_bind complement(22303..22321)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 22389..22493
/gene="bla"
/label=AmpR promoter
CDS 22494..23354
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(22712..22731)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 23525..24113
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 24014..24033
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
polyA_signal 24344..24568
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind 24557..24574
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
CDS complement(25230..25295)
/codon_start=1
/product="2A peptide from foot-and-mouth disease virus
polyprotein"
/label=F2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="VKQTLNFDLLKLAGDVESNPGP"
intron 26288..27296
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
promoter complement(27297..27572)
/label=chicken beta-actin promoter
enhancer 27575..75
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat taataactag
61 tcaataatca atgtcgaggg cgcgccgaac aaacgaccca acaccgtgcg ttttattctg
121 tctttttatt gccgtcatag cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt
181 tagcctcccc ctagggtggg cgaagaactc cagcatgaga tccccgcgct ggaggatcat
241 ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg cggcggtgga
301 atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga accccagagt
361 cccgcggccg cgtcgacatt taaatttatg ctcttttggc atgcaacatt tcaatgagaa
421 ggttattata gggcacatcc ccgttcaggt gcttgtagta gaggtattct tcagcctgca
481 tactgatggc ccggatttcg ggtagtcgaa gaagtagctg tccaaatttc tctgtctgct
541 gcgggtagtt acacattgtg tagtccagca gggcggcatt gacttgttcc tggacacctt
601 ctaccagctg gaagttttca aggtttttga catctaaact aaagagcacc aagaatttca
661 gacatacgaa ctctcgttga tcaaactgga gagaacgaag ttttgccact aactcctgtg
721 catgactcat gaggttgttg agggtggctc cggcttgtga tgctattatg gaatagtcca
781 cttgttgccc agtaaccagg aagatggatc cttcctttcc atgtaccact tgtcggtaaa
841 tgtggtcgag gattaagagc tcactccagc agttctgaag cagcttcatt tggtcatcaa
901 ccttaagttc tctgaagaag atactactcc tggcccactc gacaatggag aagagagttt
961 gatctgccat tttgcacata agcccaaagg tgctcagctt ttcgtgcttg cttcggttag
1021 cctgctcttg ctgcaaatag gccatgattt tagcctggac ttgaggctca tctggctcac
1081 acttcaaaag ttccagtatc agatgtggga tgcttgctgg agagctcgtc tggtaactat
1141 ccatatatga atagcccatt atggactcgg gtgagctggt ataggggtct gggtactcag
1201 acttgatggc ccggctagga aagtggccat atgtttggta accttgcagg ctgccgtgag
1261 ggggcattgt catgctaatg ggggatgtta caaagggact tctgtcatag tctgtaggag
1321 gcaaggcagc atggttcaga ggtaggcctt tggaggcaga gtggatgttt tgaattgcag
1381 aggaaatggt caggtcagag ggcatagctt ggatcacctg agacatggct tctagcttaa
1441 gtccattggc tcggatgagg gcttttttct gttgcttcag ggccctgtct ctcttgtaca
1501 ttggcccaaa cttattcctt cctccacgca ttcggtcggc ccttacagct tctagcttca
1561 ttccaacact tagacatttt tgaaaacgac agtaaggaca acgctttctc tgtgttttgt
1621 caatttggca gttctggttt tctatacatg tgtacctttt attattttgg actgttcgct
1681 taaaaaatcc cttgcagctt tcacaggtga ggagcccata atggtaccca gacactttat
1741 ctccacacac gggacaaagc tcttccagat cttcatcata ggagtaattc accattttaa
1801 attgagacac aataggtgta agtccgtgct ttaaagactc ttgtaaatcc ccagtatctg
1861 aattagaaga catggtggcg atcgctagct tcacgacacc tgaaatggaa gaaaaaaact
1921 ttgaaccact gtctgaggct tgagaatgaa ccaagatcca aactcaaaaa gggcaaattc
1981 caaggagaat tacatcaagt gccaagctgg cctaacttca gtctccaccc actcagtgtg
2041 gggaaactcc atcgcataaa acccctcccc ccaacctaaa gacgacgtac tccaaaagct
2101 cgagaactaa tcgaggtgcc tggacggcgc ccggtactcc gtggagtcac atgaagcgac
2161 ggctgaggac ggaaaggccc ttttcctttg tgtgggtgac tcacccgccc gctctcccga
2221 gcgccgcgtc ctccattttg agctccctgc agcagggccg ggaagcggcc atctttccgc
2281 tcacgcaact ggtgccgacc gggccagcct tgccgcccag ggcggggcga tacacggcgg
2341 cgcgaggcca ggcaccagag caggccggcc agcttgagac tacccccgtc cgattctcgg
2401 tggccgcgct cgcaggcccc gcctcgccga acatgtgcgc tgggacgcac gggccccgtc
2461 gccgcccgcg gccccaaaaa ccgaaatacc agtgtgcaga tcttggcccg catttacaag
2521 actatcttgc cagaaaaaaa gcgtcgcagc aggtcatcaa aaattttaaa tggctagaga
2581 cttatcgaaa gcagcgagac aggcgcgaag gtgccaccag attcgcacgc ggcggcccca
2641 gcgcccaggc caggcctcaa ctcaagcacg aggcgaaggg gctccttaag cgcaaggcct
2701 cgaactctcc cacccacttc caacccgaag ctcgggatca agaatcacgt actgcagcca
2761 ggtggaagta attcaaggca cgcaagggcc ataacccgta aagaggccag gcccgcggga
2821 accacacacg gcacttacct gtgttctggc ggcaaacccg ttgcgaaaaa gaacgttcac
2881 ggcgactact gcacttatat acggttctcc cccaccctcg ggaaaaaggc ggagccagta
2941 cacgacatca ctttcccagt ttaccccgcg ccaccttctc taggcaccgg ttcaattgcc
3001 gacccctccc cccaacttct cggggactgt gggcgatgtg cgctctgccc actgacgggc
3061 accggagcct cacgattaat cgccgcgccg aacaaacgac ccaacaccgt gcgttttatt
3121 ctgtcttttt attgccgtca tagcgcgggt tccttccggt attgtctcct tccgtgtttc
3181 agttagcctc cccctagggt gggcgaagaa ctccagcatg agatccccgc gctggaggat
3241 catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt
3301 ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag
3361 agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga
3421 gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca
3481 atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag
3541 tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca
3601 tgggtcacga cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg
3661 gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc
3721 atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc
3781 ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga
3841 gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt
3901 cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac
3961 gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc ggtcttgaca
4021 aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt
4081 gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg
4141 tgcaatccat cttgttcaat ggcggatccc atggttgtgg ccatattatc atcgtgtttt
4201 tcaaaggaaa accacgtccc cgtggttcgg ggggcctaga cgttttttta acctcgacta
4261 aacacatgta aagcatgtgc accgaggccc cagatcagat cccatacaat ggggtacctt
4321 ctgggcatcc ttcagcccct tgttgaatac gcttgaggag agccatttga ctctttccac
4381 aactatccaa ctcacaacgt ggcactgggg ttgtgccgcc tttgcaggtg tatcttatac
4441 acgtggcttt tggccgcaga ggcacctgtc gccaggtggg gggttccgct gcctgcaaag
4501 ggtcgctaca gacgttgttt gtcttcaaga agcttccaga ggaactgctt ccttcacgac
4561 attcaacaga ccttgcattc ctttggcgag aggggaaaga cccctaggaa tgctcgtcaa
4621 gaagacaggg ccaggtttcc gggccctcac attgccaaaa gacggcaata tggtggaaaa
4681 taacatatag acaaacgcac accggcctta ttccaagcgg cttcggccag taacgttagg
4741 ggggggggag ggagaggggc ggatccgcgg ccgctcagtt agcctccccc atctcccggg
4801 caaacgtgcg cgccaggtcg catatcgtcg gtatggagcc gggggtggtg acgtgggtct
4861 ggaccatccc ggaggtaagt tgcagcaggg cgtcccggca gccggcgggc gattggtcgt
4921 aatccaggat aaagacgtgc atggaacgga ggcgtttggc caagacgtcc aaggcccagg
4981 caaacacgtt atacaggtcg ccgttggggg ccagcaactc gggggcccga aacagggtaa
5041 ataacgtgtc cccgatatgg ggtcgtgggc ccgcgttgct ctggggctcg gcaccctggg
5101 gcggcacggc cgtccccgaa agctgtcccc agtcctcccg ccacgacccg ccgcactgca
5161 gataccgcac cgtattggca agtagcccgt aaacgcggcg aatcgcagcc agcatagcca
5221 ggtccagccg ctcgccgggg cgctggcgtt tggccaggcg gtcgatgtgt ctgtcctccg
5281 gaagggcccc aagcacgatg ttggtgccgg gcaaggtcgg cgggatgagg gccacgaacg
5341 ccagcacggc ctggggggtc atgctgccca taaggtaccg cgcggccggg tagcacagga
5401 gggcggcgat gggatggcgg tcgaagatga gggtgagggc cgggggcggg gcatgtgagc
5461 tcccagcctc ccccccgata tgaggagcca gaacggcgtc ggtcacggca taaggcatgc
5521 ccattgttat ctgggcgctt gtcattacca ccgccgcgtc cccggccgat atctcaccct
5581 ggtcgaggcg gtgttgtgtg gtgtagatgt tcgcgattgt ctcggaagcc cccagcaccc
5641 gccagtaagt catcggctcg ggtacgtaga cgatatcgtc gcgcgaaccc agggccacca
5701 gcagttgcgt ggtggtggtt ttccccatcc cgtggggacc gtctatataa acccgcagta
5761 gcgtgggcat tttctgctcc gggcggactt ccgtggcttc ttgctgccgg cgagggcgca
5821 acgccgtacg tcggttgcta tggccgcgag aacgcgcagc ctggtcgaac gcagacgcgt
5881 gttgatggcc ggggtacgaa gccatattac cctgttatcc ctagaattct ttgccaaaat
5941 gatgagacag cacaataacc agcacgttgc ccaggagctg taggaaaaag aagaaggcat
6001 gaacatggtt agcagaggct ctagagccgc cggtcacacg ccagaagccg aaccccgccc
6061 tgccccgtcc cccccgaagg cagccgtccc cctgcggcag ccccgaggct ggagatggag
6121 aaggggacgg cggcgcggcg acgcacgaag gccctccccg cccatttcct tcctgccggc
6181 gccgcaccgc ttcgcccgcg cccgctagag ggggtgcggc ggcgcctccc agatttcggc
6241 tccgccagat ttgggacaaa ggaagtccct gcgccctctc gcacgattac cataaaaggc
6301 aatggctgcg gctcgccgcg cctcgacagc cgccggcgct ccggggccgc cgcgcccctc
6361 ccccgagccc tccccggccc gaggcggccc cgccccgccc ggcaccccca cctgccgcca
6421 ccccccgccc ggcacggcga gccccgcgcc acgccccgca cggagccccg cacccgaagc
6481 cgggccgtgc tcagcaactc ggggaggggg gtgcaggggg gggttacagc ccgaccgccg
6541 cgcccacacc ccctgctcac ccccccacgc acacaccccg cacgcagcct ttgttcccct
6601 cgcagccccc ccgcaccgcg gggcaccgcc cccggccgcg ctcccctcgc gcacacgcgg
6661 agcgcacaaa gccccgcgcc gcgcccgcag cgctcacagc cgccgggcag cgcgggccgc
6721 acgcggcgct ccccacgcac acacacacgc acgcaccccc cgagccgctc ccccccgcac
6781 aaagggccct cccggagccc tttaaggctt tcacgcagcc acagaaaaga aacgagccgt
6841 cattaaacca agcgctaatt acagcccgga ggagaagggc cgtcccgccc gctcacctgt
6901 gggagtaacg cggtcagtca gagccggggc gggcggcgcg aggcggcgcg gagcggggca
6961 cggggcgaag gcaacgcagc gactcccgcc cgccgcgcgc ttcgcttttt atagggccgc
7021 cgccgccgcc gcctcgccat aaaaggaaac tttcggagcg cgccgctctg attggctgcc
7081 gccgcacctc tccgcctcgc cccgccccgc ccctcgcccc gccccgcccc gcctggcgcg
7141 cgcccccccc ccccccgccc ccatcgctgc acaaaataat taaaaaataa ataaatacaa
7201 aattgggggt ggggaggggg gggagatggg gagagtgaag cagaacgtgg ggctcacctc
7261 gacccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata
7321 aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg
7381 gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatagt
7441 ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta
7501 ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt
7561 atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt
7621 aataactagc gctgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
7681 aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
7741 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
7801 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
7861 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
7921 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
7981 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
8041 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
8101 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta cgccttaaga
8161 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
8221 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
8281 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
8341 agcaagtaaa acctctacaa atgtggtatg gctgattatg atctagagtc gcggccgcta
8401 ttacgcacaa gagttccgta gctgttcaag tttgtgtttc aactgttctc gtcgtttccg
8461 caacaagtcc tcttcagaaa tgagcttttg ctcctctgct tggacggaca ggatgtatgc
8521 tgtggctttt ttaaggataa ctaccttggg ggccttttca ttgttttcca actccgggat
8581 ctggtcacgc agggcaaaaa agctccgttt tagctcgttc ctcctctggc gctccaagac
8641 gttgtgtgtt cgcctcttga cattctcctc ggtgtccgag gacctggggc tggtgcattt
8701 tcggttgttg ctgatctgtc tcaggactct gacactgtcc aacttgaccc tcttggcagc
8761 aggatagtcc ttccgagtgg agggaggcgc tgcgtagttg tgctgatgtg tggagacgtg
8821 gcacctcttg aggaccagtg ggctgtgagg aggtttgctg tggcctccag cagaaggtga
8881 tccagactct gaccttttgc caggagcctg cctcttttcc acagaaacaa catcgatttc
8941 ttcctcatct tcttgttcct cctcagagtc gctgctggtg gtgggcggtg tctcctcatg
9001 gagcaccagg ggctcggggc tgccctgcgg ggaggactcc gtcgaggaga gcagagaatc
9061 cgaggacgga gagaaggcgc tggagtcttg cgaggcgcag gacttgggcg agctgctgtc
9121 gttgagaggg taggggaaga ccaccgaggg gtcgatgcac tctgaggcgg cggcgctcag
9181 atcctgcagg tacaagctgg aggtggagca gacgctgtgg ccgcgggcgg ggttcgggct
9241 gccgctgtct ttgcgcgcag cctggtagga ggccagcttc tctgagacga gcttggcggc
9301 ggccgagaag ccgctccaca tacagtcctg gatgatgatg tttttgatga aggtctcgtc
9361 gtccgggtcg cagatgaaac tctggttcac catgtctcct cccagcagct cggtcaccat
9421 ctccagctgg tcggccgtgg agaagctccc gccaccgccg tcgttgtctc cccgaaggga
9481 gaagggtgtg accgcaacgt aggagggcga gcagagcccg gagcggcggc taggggacag
9541 gggcggggtg ggcagcagct cgaatttctt ccagatatcc tcgctgggcg ccgggggctg
9601 cagctcgctc tgctgctgct gctggtagaa gttctcctcc tcgtcgcagt agaaatacgg
9661 ctgcaccgag tcgtagtcga ggtcatagtt cctgttggtg aagctaacgt tgaggggcat
9721 aggaccgggg ttactttcaa catcgccagc gagtttcaac aaagcgtagt tagtacattg
9781 cccactaccc atgtgtgaga ggggcagtgt gccgttaatg gccgtgccgg gcaccgggcc
9841 gctctggtag tgctgggaca tgtgaagtct gctgggggcg gcgggttccg gcacctcggc
9901 gccggggaga tacatgctga tcatgtcccg gaggtccccg gcctggcagg gcgccctgga
9961 gtgggaggaa gaggtaacca caggggggct ggagctggcc tcggacttga ccaccgaacc
10021 catggagcca agagccatgc caggggtgcc ctgctgcgag taggacatgc tgtaggtggg
10081 cgagccgttc atgtaggtct gcgagctggt catggagttg tactgcaggg cgctcacgtc
10141 gtagcggtgc atgggctgca tctgcgctgc gccgtgcgca ttgaggcccg ggtgctgcgg
10201 gtagcccagc tggtcctgca tcatgctgta gctgccgttg ctccagccgt tcatgtgcgc
10261 gtaactgtcc atgcgctggt tcacgcccgc gcccaggccg gcgcccaccc cgaccccgct
10321 cgccatgcta ttgccgccgg gggccagcag cccgccgggc agcgtgtact tatccttctt
10381 catgagcgtc ttggttttcc gccggggccg gtatttataa tccgggtgct ccttcatgtg
10441 cagcgctcgc agccgcttag cctcgtcgat gaacggccgc ttctccgtct ccgacaaaag
10501 tttccactcg gcgcccaggc gcttgctgat ctccgagttg tgcatcttgg ggttctcctg
10561 ggccatcttg cgccgctgcc cgcgggacca caccatgaag gcattcatgg gccgcttgac
10621 gcggtccggg ctgtttttct ggttgccgcc ggccgccgcc gcggtggagt tgccgccgcc
10681 gccccccgaa gtttgctgcg ggcccggcgg cttcagctcc gtctccatca tgttgtacat
10741 ggggccgggg ttctcctcca cgtcgccgca ggtcagcagg ctgcccctgc cctcgccgct
10801 gccaaaatgc ctcttcatgt gtaaggcgag gtggtccgac ctggaaaatg ctcggtcgca
10861 tttttggcac tggaacgggc ggtgccccgt gtgtttacgg tagtgcctgg tcagttcatc
10921 tgagcgggcg aatttccatc cacagccgtc ccagtcacag tggtaaggtt tctcacctgt
10981 gtgggttcgc aggtgtgcct tgagatggga actctttgtg taggttttgc cgcagcccgc
11041 gtaatcacaa gtgtgggtgg cggtcctttt ccggggccac gatcgtcttc ccctctttgg
11101 cttgggctcc tctggcatgc aggaaccggg tggcatgagc tcttggtaat ggagcggcgg
11161 gacttgcggc tgcatctgat cgggcaggaa ggatgggtaa ttgggccccg ggtggggatg
11221 gaagccggga ggaagcggca gggcagggtg acagtccctg ctgctcagca cttcctcaag
11281 acccagggtc ggggtagtcc tgctggggag ctgccgcccc agggggaagt cgtgtgcagc
11341 cggccggtgg ccattgctga gagggggtcc agcgcccaag tgggtgcacg aagagaccgc
11401 ctcctgcttg atcttggggc acgtgcgcgg cggcccgccg ttgtagggcg ccaccaccac
11461 cgggtggctg ccgtcagggc tgcctttgct gacgctgatg accgacgggc tgccgtactc
11521 gctgccaggg gcgctcagcg acgccttcag cacgaacttg cccatcagcc cgccacctgg
11581 cggctgcggc tgctgcggcg gaatgtacac cgggtccaat tctggccgca ggagctcggc
11641 cacgaagccg cccgaggggc tcacgtcgtt gatgtccgcc aggttgaagg gagccgtcgg
11701 agggggagcg gactccctgc catagaggag gcctccgccc gtgccgcccg gcgccacgcc
11761 cgggtcgttc ccggcccgga tcggataggt gaagctgcag gtggagggcg cgctggcagg
11821 gccgctgctc gacggcgacg acgaagagga ggctgacgct gacgaggaca cggtggcggc
11881 cactgactcc ggaggatggg tcagcgaatt ggagagaata aagtccaggt ccaggagatc
11941 gttgaactcc tcggtctctc tccgaggtag gggcgccagg ttgctaccgc cgcaagccgc
12001 accggctccg ccgctctcca ggtctgtggc cacggtcgcc gccgccaggt cataggggcg
12061 gccgggaagc actgggggaa gtcgcttcat gtgggagagc tcctcccgcc agcggttatt
12121 cggggcacct gcttgacgca gtgtcttctc ccttcccgcc gggccagacg cgaacgtgga
12181 gaaagatggg agcagcgcgt cgctgacagc catgggccct gggttggact ccacgtctcc
12241 cgccaacttg agaaggtcaa aattcaaagt ctgtttcacg ccacttccgt ttgaatgcat
12301 gggagagccc agagtggtga cggagacagg gggaaaggct tccccctcag ggaaagggac
12361 cgaggagtac agtgcagtga agtgagggct cccatagcct ggggtaccaa aatggggccc
12421 tggggccaga ggaaaggaca ctggtccccc tgagaaagga gacccagcag cctcaaaatc
12481 ctctcgttgt gcatagtcgc tgcttgatcg cttgcccttc tggcgccggt tacagaacca
12541 cactcggacc acatccttct cgagcccaag ctgctgggcg atgtggctga tctgctgcag
12601 tgtgggtttc gggcactgca ggaacaaatt ctccaggttg cctctcactc ggttctcgat
12661 actggttcgc tttctctttc gggcctgcac gagggtttct gctttgcata tctcctgaag
12721 attttcattg ttgtcagctt cctccaccca cttctgcagc aagggccgca gcttacacat
12781 gttcttgaag ctaagctgca gagcctcaaa gcggcagatg gtcgtttggc tgaatacctt
12841 cccaaataga acccccaggg tgagccccac atcggcctgt gtatatccca gggtgatcct
12901 cttctgcttc aggagcttgg caaattgctc gagttctttc tgcagagctt tgatgtcctg
12961 ggactcctcc gggttttgct ccagcttctc cttctccagc ttcacggcac caggggtgac
13021 ggtgcagggc tccggggagg ccccatcgga gttgctctcc accccgactc ctgcttcgcc
13081 ctcaggctga gaggtctcca agccgccttg gggcactagc cccactccaa cctggggccc
13141 acagtacgcc atccccccac agaactcata cggcgggggg catgggggaa tcccccacac
13201 ctcagagcct ggcccaaccc ccggcccgat tcctggccct ccaggagggc cttggaagct
13261 tagccaggtc cgaggatcaa cccagcccgg ctccggcccc cctggcccat cacctccacc
13321 acctggaggg ggcgagaagg cgaaatccga agccaggtgt cccgccatgg tggcgctagc
13381 ttcacgacac ctgaaatgga agaaaaaaac tttgaaccac tgtctgaggc ttgagaatga
13441 accaagatcc aaactcaaaa agggcaaatt ccaaggagaa ttacatcaag tgccaagctg
13501 gcctaacttc agtctccacc cactcagtgt ggggaaactc catcgcataa aacccctccc
13561 cccaacctaa agacgacgta ctccaaaagc tcgagaacta atcgaggtgc ctggacggcg
13621 cccggtactc cgtggagtca catgaagcga cggctgagga cggaaaggcc cttttccttt
13681 gtgtgggtga ctcacccgcc cgctctcccg agcgccgcgt cctccatttt gagctccctg
13741 cagcagggcc gggaagcggc catctttccg ctcacgcaac tggtgccgac cgggccagcc
13801 ttgccgccca gggcggggcg atacacggcg gcgcgaggcc aggcaccaga gcaggccggc
13861 cagcttgaga ctacccccgt ccgattctcg gtggccgcgc tcgcaggccc cgcctcgccg
13921 aacatgtgcg ctgggacgca cgggccccgt cgccgcccgc ggccccaaaa accgaaatac
13981 cagtgtgcag atcttggccc gcatttacaa gactatcttg ccagaaaaaa agcgtcgcag
14041 caggtcatca aaaattttaa atggctagag acttatcgaa agcagcgaga caggcgcgaa
14101 ggtgccacca gattcgcacg cggcggcccc agcgcccagg ccaggcctca actcaagcac
14161 gaggcgaagg ggctccttaa gcgcaaggcc tcgaactctc ccacccactt ccaacccgaa
14221 gctcgggatc aagaatcacg tactgcagcc aggtggaagt aattcaaggc acgcaagggc
14281 cataacccgt aaagaggcca ggcccgcggg aaccacacac ggcacttacc tgtgttctgg
14341 cggcaaaccc gttgcgaaaa agaacgttca cggcgactac tgcacttata tacggttctc
14401 ccccaccctc gggaaaaagg cggagccagt acacgacatc actttcccag tttaccccgc
14461 gccaccttct ctaggcaccg gttcaattgc cgacccctcc ccccaacttc tcggggactg
14521 tgggcgatgt gcgctctgcc cactgacggg caccggagcc tcacgattaa ttaataggga
14581 taacagggta atttaaatag atctactagt tattaatagt aatcaattac ggggtcatta
14641 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
14701 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
14761 ccaataggga ctttccattg acgtcaatgg gtggactatt tacggtaaac tgcccacttg
14821 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
14881 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
14941 atctacgtat tagtcatcgc tattaccatg ggtcgaggtg agccccacgt tctgcttcac
15001 tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt
15061 ttgtgcagcg atgggggcgg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg
15121 aggggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc
15181 gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc
15241 ggcgggcggg agtcgctgcg ttgccttcgc cccgtgcccc gctccgcgcc gcctcgcgcc
15301 gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt
15361 ctcctccggg ctgtaattag cgcttggttt aatgacggct cgtttctttt ctgtggctgc
15421 gtgaaagcct taaagggctc cgggagggcc ctttgtgcgg gggggagcgg ctcggggggt
15481 gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggcc cgcgctgccc ggcggctgtg
15541 agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcgt gtgcgcgagg ggagcgcggc
15601 cgggggcggt gccccgcggt gcgggggggc tgcgagggga acaaaggctg cgtgcggggt
15661 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcggcgg tcgggctgta acccccccct
15721 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtgcggg
15781 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
15841 ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc ccggagcgcc
15901 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
15961 gcgcagggac ttcctttgtc ccaaatctgg cggagccgaa atctgggagg cgccgccgca
16021 ccccctctag cgggcgcggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
16081 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccat ctccagcctc ggggctgccg
16141 cagggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
16201 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
16261 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt cgctagtcga
16321 cgagcggccg cagctctaaa tactctgaag tccaaagaag ttgtatgttg ggtgggctcc
16381 cttcaacttt aacatggaag tgctttctgt gactttaaaa gtaagtgctt ccatgtttta
16441 gtaggagtga atccaattta cttctccaaa atagaacacg ctaacctcat ttgaagggat
16501 cccctttgct ttaacatggg ggtacctgct gtgtgaaaca aaagtaagtg cttccatgtt
16561 tcagtggagg tgtctccaag ccagcacacc ttttgttaca aaattttttt gttattgtgt
16621 tttaaggtta ctaagcttgt tacaggttaa aggattctaa ctttttccaa gactgggctc
16681 cccaccactt aaacgtggat gtacttgctt tgaaactaaa gaagtaagtg cttccatgtt
16741 ttggtgatgg taagtcttcc ttttacattt ttattatttt tttagaaaat aactttattg
16801 tattgaccgc agctcatata tttaagcttt attttgtatt tttacatctg ttaaggggcc
16861 ccctctactt taacatggag gcacttgctg tgacatgaca aaaataagtg cttccatgtt
16921 tgagtgtggt ggttcctacc taatcagcaa ttgcgttaac gcccacactg tgtgcagttc
16981 ttggctacag gccattactg ttgctaatat gcaactctgt tgaatataaa ttggaattgc
17041 actttagcaa tggtgatgga ttgttaagcc aatgacagaa tttaaaccac agacttactt
17101 tgatagcact cttaatggta taacttcttc tcccaggcgc gtctagatca taatcagcca
17161 taccacattg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
17221 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
17281 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
17341 tgtggtttgt ccaaactcat caatgtatct taaagacgtc tttaattaat cgactctaga
17401 ggatcgatgc cccgccccgg acgaactaaa cctgactacg acatctctgc cccttcttcg
17461 cggggcagtg catgtaatcc cttcagttgg ttggtacaac ttgccaactg ggccctgttc
17521 cacatgtgac acgggggggg accaaacaca aaggggttct ctgactgtag ttgacatcct
17581 tataaatgga tgtgcacatt tgccaacact gagtggcttt catcctggag cagactttgc
17641 agtctgtgga ctgcaacaca acattgcctt tatgtgtaac tcttggctga agctcttaca
17701 ccaatgctgg gggacatgta cctcccaggg gcccaggaag actacgggag gctacaccaa
17761 cgtcaatcag aggggcctgt gtagctaccg ataagcggac cctcaagagg gcattagcaa
17821 tagtgtttat aaggccccct tgttaaccct aaacgggtag catatgcttc ccgggtagta
17881 gtatatacta tccagactaa ccctaattca atagcatatg ttacccaacg ggaagcatat
17941 gctatcgaat tagggttagt aaaagggtcc taaggaacag cgatatctcc caccccatga
18001 gctgtcacgg ttttatttac atggggtcag gattccacga gggtagtgaa ccattttagt
18061 cacaagggca gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa tcttcgcctg
18121 cttcttcatt ctccttcgtt tagctaatag aataactgct gagttgtgaa cagtaaggtg
18181 tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa tttggacggg
18241 gggttcagtg gtggcattgt gctatgacac caatataacc ctcacaaacc ccttgggcaa
18301 taaatactag tgtaggaatg aaacattctg aatatcttta acaatagaaa tccatggggt
18361 ggggacaagc cgtaaagact ggatgtccat ctcacacgaa tttatggcta tgggcaacac
18421 ataatcctag tgcaatatga tactggggtt attaagatgt gtcccaggca gggaccaaga
18481 caggtgaacc atgttgttac actctatttg taacaagggg aaagagagtg gacgccgaca
18541 gcagcggact ccactggttg tctctaacac ccccgaaaat taaacggggc tccacgccaa
18601 tggggcccat aaacaaagac aagtggccac tctttttttt gaaattgtgg agtgggggca
18661 cgcgtcagcc cccacacgcc gccctgcggt tttggactgt aaaataaggg tgtaataact
18721 tggctgattg taaccccgct aaccactgcg gtcaaaccac ttgcccacaa aaccactaat
18781 ggcaccccgg ggaatacctg cataagtagg tgggcgggcc aagatagggg cgcgattgct
18841 gcgatctgga ggacaaatta cacacacttg cgcctgagcg ccaagcacag ggttgttggt
18901 cctcatattc acgaggtcgc tgagagcacg gtgggctaat gttgccatgg gtagcatata
18961 ctacccaaat atctggatag catatgctat cctaatctat atctgggtag cataggctat
19021 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
19081 cctaatttat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
19141 cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat
19201 cctaatagag attagggtag tatatgctat cctaatttat atctgggtag catatactac
19261 ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata tgctatccta
19321 atctatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
19381 atctatatct gggtagtata tgctatccta atttatatct gggtagcata ggctatccta
19441 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
19501 atctgtatcc gggtagcata tgctatcctc atgcatatac agtcagcata tgatacccag
19561 tagtagagtg ggagtgctat cctttgcata tgccgccacc tcccaagggg gcgtgaattt
19621 tcgctgcttg tccttttcct gctggttgct cccattctta ggtgaattta aggaggccag
19681 gctaaagccg tcgcatgtct gattgctcac caggtaaatg tcgctaatgt tttccaacgc
19741 gagaaggtgt tgagcgcgga gctgagtgac gtgacaacat gggtatgccc aattgcccca
19801 tgttgggagg acgaaaatgg tgacaagaca gatggccaga aatacaccaa cagcacgcat
19861 gatgtctact ggggatttat tctttagtgc gggggaatac acggctttta atacgattga
19921 gggcgtctcc taacaagtta catcactcct gcccttcctc accctcatct ccatcacctc
19981 cttcatctcc gtcatctccg tcatcaccct ccgcggcagc cccttccacc ataggtggaa
20041 accagggagg caaatctact ccatcgtcaa agctgcacac agtcaccctg atattgcagg
20101 taggagcggg ctttgtcata acaaggtcct taatcgcatc cttcaaaacc tcagcaaata
20161 tatgagtttg taaaaagacc atgaaataac agacaatgga ctcccttagc gggccaggtt
20221 gtgggccggg tccaggggcc attccaaagg ggagacgact caatggtgta agacgacatt
20281 gtggaatagc aagggcagtt cctcgcctta ggttgtaaag ggaggtctta ctacctccat
20341 atacgaacac accggcgacc caagttcctt cgtcggtagt cctttctacg tgactcctag
20401 ccaggagagc tcttaaacct tctgcaatgt tctcaaattt cgggttggaa cctccttgac
20461 cacgatgctt tccaaaccac cctccttttt tgcgcctgcc tccatcaccc tgaccccggg
20521 gtccagtgct tgggccttct cctgggtcat ctgcggggcc ctgctctatc gctcccgggg
20581 gcacgtcagg ctcaccatct gggccacctt cttggtggta ttcaaaataa tcggcttccc
20641 ctacagggtg gaaaaatggc cttctacctg gagggggcct gcgcggtgga gacccggatg
20701 atgatgactg actactggga ctcctgggcc tcttttctcc acgtccacga cctctccccc
20761 tggctctttc acgacttccc cccctggctc tttcacgtcc tctaccccgg cggcctccac
20821 tacctcctcg accccggcct ccactacctc ctcgaccccg gcctccactg cctcctcgac
20881 cccggcctcc acctcctgct cctgcccctc ctgctcctgc ccctcctcct gctcctgccc
20941 ctcctgcccc tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgcccctc
21001 ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctcctgctc
21061 ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg
21121 cccctcctgc tcctgcccct cctgctcctg cccctcctgc tcctgcccct cctgctcctg
21181 cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgctcct gcccctcctg
21241 cccctcctgc ccctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg
21301 cccctcctcc tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctcctgctc
21361 ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctcctg
21421 ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg
21481 cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc tcctgcccct cctcctgctc
21541 ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc tcccgctcct gctcctgctc
21601 ctgttccacc gtgggtccct ttgcagccaa tgcaacttgg acgtttttgg ggtctccgga
21661 caccatctct atgtcttggc cctgatcctg agccgcccgg ggctcctggt cttccgcctc
21721 ctcgtcctcg tcctcttccc cgtcctcgtc catggttatc accccctctt ctttgaggtc
21781 cactgccgcc ggagccttct ggtccagatg tgtctccctt ctctcctagg ccatttccag
21841 gtcctgtacc tggcccctcg tcagacatga ttcacactaa aagagatcaa tagacatctt
21901 tattagacga cgctcagtga atacagggag tgcagactcc tgccccctcc aacagccccc
21961 ccaccctcat ccccttcatg gtcgctgtca gacagatcca ggtctgaaaa ttccccatcc
22021 tccgaaccat cctcgtcctc atcaccaatt actcgcagcc cggaaaactc ccgctgaaca
22081 tcctcaagat ttgcgtcctg agcctcaagc caggcctcaa attcctcgtc cccctttttg
22141 ctggacggta gggatgggga ttctcgggac ccctcctctt cctcttcaag gtcaccagac
22201 agagatgcta ctggggcaac ggaagaaaag ctgggtgcgg cctgtgagga tcagcttatc
22261 gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat
22321 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
22381 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
22441 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
22501 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
22561 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
22621 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
22681 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
22741 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
22801 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
22861 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
22921 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
22981 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag
23041 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
23101 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
23161 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
23221 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
23281 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
23341 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
23401 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
23461 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
23521 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
23581 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
23641 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
23701 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
23761 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
23821 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
23881 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
23941 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
24001 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
24061 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
24121 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttgaagctgt ccctgatggt
24181 cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag
24241 cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgagcgat taaacgcgcc
24301 aactagagga tcgagcccca gctggttctt tccgcctcag aagccataga gcccaccgca
24361 tccccagcat gcctgctatt gtcttcccaa tcctccccct tgctgtcctg ccccacccca
24421 ccccccagaa tagaatgaca cctactcaga caatgcgatg caatttcctc attttattag
24481 gaaaggacag tgggagtggc accttccagg gtcaaggaag gcacggggga ggggcaaaca
24541 acagatggct ggcaactaga aggcacagtc gaggctgatc agcgagctcg tttatcttat
24601 caattctgtg cctccgggag cagggtaggg ctgtggattt cttcttcttc ctctcgaaag
24661 taggttggct ttccctgtgc actagggccc tgctgggcct tcagcggaca tgaggctacc
24721 atatggctga tgctctggca gaagtggcac ttcttgggct ggggtggcag cttgcattcc
24781 ttggcatgat gatctagacc tccacagttg tagcacctgt ctccttttga tctgcgcttc
24841 tgcatgctct ttccttttgg ccgcctctca ctcccaatac agaatactcc accaggtccg
24901 gtgacacgga tggattccag acccttggct gacttcttaa aggtgaactc cactgcctca
24961 ccctccttca agctccggaa cccttccatg tgcagcttac tctggtgcac aaagacatcc
25021 actggggggt cgagcgcgac cccggcgcgg gcggtcatgg acaggaagcc gaaccccatg
25081 cgcacgttga accacttaca gatgcccgca ccgtgcagca gctgaggctc gtccgccgcc
25141 cgggccgcgt cctccggcgc ctcctcgggc gcctcttctg ccgccttggc gcagccacct
25201 gcaaactgct ggttggacac ggagcccatg ggccctgggt tggactccac gtctcccgcc
25261 aacttgagaa ggtcaaaatt caaagtctgt ttcacgccac ttcccacgtc ttcaggttgc
25321 atgttcatgg agtagtttag gaataaatcc atggtttgtg gagtactaaa atacctagtg
25381 gtctgctgta ttacattaag gccttcccca gcagcttcca aggcagcctc caagtcactg
25441 gcaggagaat ttggctggaa ctgcatgcag gactgcagag attcctctcc acagttatag
25501 aagggactgt tccaggcctg attgttccag gattgggtgc accaggtctg agtgttccag
25561 gagtggttgc tccaggactg gatgttctgg gtctggttgc tccaggttga attgttccag
25621 gtctggttgc tccacattgg aaggttccca gtcgggttca ccaggcatcc ctggtggtag
25681 gaagagtaaa ggctggggta ggtaggtgct gaggccttct gcgtcacacc attgctattc
25741 ttcggccagt tgtttttctg ccacctctta gatttcattc tctggttctg gaaccaggtc
25801 ttcacctgtt tgtagctgag gttcaggatg ttggagagtt cttgcatctg ctggaggctg
25861 aggtatttct gtctctgaaa tctatcattg agtacacaca gctgggtgga agagaacaca
25921 gttctggtct tctgtttctt gaccgggacc ttgtcttcct tttttgcgac actcttctct
25981 gcagaagtgg gttgtttgcc tttgggactg gtggaagaat cagggctgtc ctgaataagc
26041 agatccatgg aggaaggaag aggagagaca gtctccgtgt gaggcatctc agcagaagac
26101 atttgcaagg atggatagtt ttcttcaggc ccacaaatca caggcatagg tgaagattct
26161 ttacagtcgg atgcttcaaa gcaaggcaag ctttggggac aagctggatc cacactcatg
26221 gtggcttaat cgcgaattct ttgccaaaat gatgagacag cacaataacc agcacgttgc
26281 ccaggagctg taggaaaaag aagaaggcat gaacatggtt agcagaggct ctagagccgc
26341 cggtcacacg ccagaagccg aaccccgccc tgccccgtcc cccccgaagg cagccgtccc
26401 cctgcggcag ccccgaggct ggagatggag aaggggacgg cggcgcggcg acgcacgaag
26461 gccctccccg cccatttcct tcctgccggc gccgcaccgc ttcgcccgcg cccgctagag
26521 ggggtgcggc ggcgcctccc agatttcggc tccgccagat ttgggacaaa ggaagtccct
26581 gcgccctctc gcacgattac cataaaaggc aatggctgcg gctcgccgcg cctcgacagc
26641 cgccggcgct ccggggccgc cgcgcccctc ccccgagccc tccccggccc gaggcggccc
26701 cgccccgccc ggcaccccca cctgccgcca ccccccgccc ggcacggcga gccccgcgcc
26761 acgccccgca cggagccccg cacccgaagc cgggccgtgc tcagcaactc ggggaggggg
26821 gtgcaggggg gggttacagc ccgaccgccg cgcccacacc ccctgctcac ccccccacgc
26881 acacaccccg cacgcagcct ttgttcccct cgcagccccc ccgcaccgcg gggcaccgcc
26941 cccggccgcg ctcccctcgc gcacacgcgg agcgcacaaa gccccgcgcc gcgcccgcag
27001 cgctcacagc cgccgggcag cgcgggccgc acgcggcgct ccccacgcac acacacacgc
27061 acgcaccccc cgagccgctc ccccccgcac aaagggccct cccggagccc tttaaggctt
27121 tcacgcagcc acagaaaaga aacgagccgt cattaaacca agcgctaatt acagcccgga
27181 ggagaagggc cgtcccgccc gctcacctgt gggagtaacg cggtcagtca gagccggggc
27241 gggcggcgcg aggcggcgcg gagcggggca cggggcgaag gcaacgcagc gactcccgcc
27301 cgccgcgcgc ttcgcttttt atagggccgc cgccgccgcc gcctcgccat aaaaggaaac
27361 tttcggagcg cgccgctctg attggctgcc gccgcacctc tccgcctcgc cccgccccgc
27421 ccctcgcccc gccccgcccc gcctggcgcg cgcccccccc ccccccgccc ccatcgctgc
27481 acaaaataat taaaaaataa ataaatacaa aattgggggt ggggaggggg gggagatggg
27541 gagagtgaag cagaacgtgg ggctcacctc gacccatggt aatagcgatg actaatacgt
27601 agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg
27661 gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta
27721 ctgccaagtg ggcagtttac cgtaaatagt ccacccattg acgtcaatgg aaagtcccta
27781 ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg
27841 gtcagccagg cgggccattt accgtaagtt atgtaacgc
//
