| 质粒名称: | pDJ657 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001156 |
| 产品用途: | Mammalian cAMP-responsive NanoLuc expression vector |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | cAMP-responsive |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | NanoLuc |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Fluorescent protein-based ticker tapes for multiplexed recordings of transcriptional histories in single cells in culture and in vivo
Wang R., Jiang J., Li Z., Liu T., Wang Y., Xie M., Piatkevich K.D.
Preprint
|
pDJ657
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pDJ657载体质粒基本信息
pDJ657质粒图谱载体图谱和pDJ657载体序列质粒序列多克隆位点信息
pDJ657质粒载体简介
pDJ657质粒序列载体序列
LOCUS pDJ657 4776 bp DNA circular SYN 10-MAR-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM recombinant plasmid
REFERENCE 1 (bases 1 to 4776)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Aug 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4776
/mol_type="other DNA"
/organism="recombinant plasmid"
primer_bind 14..41
/label=oSCL930
misc_feature 24..612
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(77..97)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(495..506)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 655..776
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(666..685)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 679..699
/label=PSG5-R(SV40-R)
primer_bind 720..739
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 881..897
/label=oSCL929
repeat_region 938..1067
/label=AAV2 ITR
primer_bind complement(984..995)
/label=L4440
/note="L4440 vector, forward primer"
primer_bind 1045..1056
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(1133..1150)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(1304..1892)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(1380..1403)
/label=pBR322ori-F
CDS complement(2063..2923)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 2686..2705
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(2924..3028)
/gene="bla"
/label=AmpR promoter
rep_origin complement(3054..3509)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3191..3212)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 3403..3422
/label=F1ori-R
/note="F1 origin, reverse primer"
repeat_region 3581..3710
/label=AAV2 ITR
primer_bind complement(3592..3603)
/label=L4440
/note="L4440 vector, forward primer"
primer_bind 3653..3664
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(3751..3770)
/label=oSCL929
primer_bind 3874..3917
/label=ODJ657-1
misc_feature 3919..4005
/label=CRE reg_Regulatory_Seq
misc_feature 3930..3937
/label=CRE
misc_feature 3966..3973
/label=CRE*
misc_feature 3988..3995
/label=CRE
promoter 4012..4158
/label=P_TAL
primer_bind 4161..4199
/label=oQZ280
primer_bind 4161..4184
/label=oQZ321
CDS 4173..4241
/codon_start=1
/label=Secretory (IgK)
/label=nonstandard type: signal
/translation="MTSETDTLLLWVLLLWVPGSTGD"
primer_bind 4173..4199
/label=oQZ331
primer_bind 4176..4197
/label=oQZ757
primer_bind complement(4243..4275)
/label=oQZ758
primer_bind 4243..4275
/label=oQZ759
CDS 4260..4775
/codon_start=1
/product="NanoLuc(R) luciferase"
/label=Nluc
/note="human codon-optimized"
/translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
RLCERILA"
primer_bind complement(4370..4393)
/label=oQZ826
primer_bind 4394..4418
/label=oQZ827
primer_bind complement(join(4754..4776,1..19))
/label=oDJ657-2
primer_bind complement(4754..4775)
/label=oQZ281
primer_bind complement(4757..4776)
/label=oQZ322
ORIGIN
1 aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
61 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
121 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
181 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
241 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
301 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
361 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
421 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
481 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
541 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
601 ccgcctcccc gcatcgatac cgtcgacccg ggcggccgct tcgagcagac atgataagat
661 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
721 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
781 acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa
841 gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tcttcctaga gcatggctac
901 gtagataagt agcatggcgg gttaatcatt aactacaagg aacccctagt gatggagttg
961 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
1021 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cattaatgaa
1081 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
1141 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
1201 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
1261 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
1321 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
1381 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
1441 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
1501 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
1561 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
1621 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
1681 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
1741 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
1801 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
1861 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
1921 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
1981 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
2041 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
2101 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
2161 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
2221 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
2281 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
2341 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
2401 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
2461 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
2521 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
2581 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
2641 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
2701 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
2761 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
2821 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
2881 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
2941 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
3001 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt
3061 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
3121 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
3181 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
3241 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
3301 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
3361 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
3421 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
3481 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg
3541 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctgcgcgctc gctcgctcac
3601 tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt ggtcgcccgg cctcagtgag
3661 cgagcgagcg cgcagagagg gagtggccaa ctccatcact aggggttcct tgtagttaat
3721 gattaacccg ccatgctact tatctacgta gccatgctct aggaagagta ccattgacgt
3781 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
3841 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atggtaccga
3901 gctcttacgc gtgctagcgc accagacagt gacgtcagct gccagatccc atggccgtca
3961 tactgtgacg tctttcagac accccattga cgtcaatggg agaacagatc tgccgccccg
4021 actgcatctg cgtgttcgaa ttcgccaatg acaagacgct gggcggggtt tgtgtcatca
4081 tagaactaaa gacatgcaaa tatatttctt ccggggacac cgccagcaaa cgcgagcaac
4141 gggccacggg gatgaagcag aagcttgcca ccatgactag tgagacagac acactcctgc
4201 tatgggtact gctgctctgg gttccaggtt ccactggtga cgctagtggt ggttctggta
4261 tggtcttcac actcgaagat ttcgttgggg actggcgaca gacagccggc tacaacctgg
4321 accaagtcct tgaacaggga ggtgtgtcca gtttgtttca gaatctcggg gtgtccgtaa
4381 ctccgatcca aaggattgtc ctgagcggtg aaaatgggct gaagatcgac atccatgtca
4441 tcatcccgta tgaaggtctg agcggcgacc aaatgggcca gatcgaaaaa atttttaagg
4501 tggtgtaccc tgtggatgat catcacttta aggtgatcct gcactatggc acactggtaa
4561 tcgacggggt tacgccgaac atgatcgact atttcggacg gccgtatgaa ggcatcgccg
4621 tgttcgacgg caaaaagatc actgtaacag ggaccctgtg gaacggcaac aaaattatcg
4681 acgagcgcct gatcaacccc gacggctccc tgctgttccg agtaaccatc aacggagtga
4741 ccggctggcg gctgtgcgaa cgcattctgg cgtgag
//
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pDJ657质粒载体应用举例
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