| 质粒名称: | pAAVLINK-3'-ARHGAP32-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001929 |
| 产品用途: | 3' AAVLINK plasmid for ARHGAP32 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: |
Promoterless
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ARHGAP32 |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-3'-ARHGAP32-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-3'-ARHGAP32-FLAG载体质粒基本信息
pAAVLINK-3'-ARHGAP32-FLAG质粒图谱载体图谱和pAAVLINK-3'-ARHGAP32-FLAG载体序列质粒序列多克隆位点信息
pAAVLINK-3'-ARHGAP32-FLAG质粒载体简介
pAAVLINK-3'-ARHGAP32-FLAG质粒序列载体序列
LOCUS pAAVLINK-3'-ARHGAP32-FLAG 6347 bp DNA circular SYN 10-AUG-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6347)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 16, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6347
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
protein_bind 145..178
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 179..270
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 271..3086
/codon_start=1
/gene="ARHGAP32"
/gene_synonym="GC-GAP; GRIT; p200RhoGAP; p250GAP;
PX-RICS;RICS"
/product="rho GTPase-activating protein 32 isoform 3"
/label=ARHGAP32
/note="isoform 3 is encoded by transcript variant 3;RhoGAP
involved in the -catenin-N-cadherin and NMDA receptor
signaling; brain-specific Rho GTP-ase-activating protein;
rho/Cdc42/Rac GTPase-activating protein RICS; rac GTPase
activating protein; GAB-associated CDC42; rho
GTPase-activating protein 32; GTPase regulator interacting
with TrkA; brain-specific Rho GTPase-activating protein;
GAB-associated Cdc42/Rac GTPase-activating protein; rhoGAP
involved in the beta-catenin-N-cadherin and NMDA receptor
signaling; rho-type GTPase-activating protein 32;
GTPase-activating protein for Cdc42 and Rac1"
/db_xref="CCDS:CCDS91623.1"
/db_xref="GeneID:9743"
/db_xref="HGNC:HGNC:17399"
/db_xref="MIM:608541"
/protein_id="NP_001364953.1"
/translation="NQPHQAYLSGDPEKARITSVPLDSEKSDDHVSFPEDQSGKNSMPT
VSFLDQDQSPPRFYSGDQPPSYLGASVDKLHHPLEFADKSPTPPNLPSDKIYPPSGSPE
ENTSTATMTYMTTTPATAQMSTKEASWDVAEQPTTADFAAATLQRTHRTNRPLPPPPSQ
RSAEQPPVVGQVQAATNIGLNNSHKVQGVVPVPERPPEPRAMDDPASAFISDSGAAAAQ
CPMATAVQPGLPEKVRDGARVPLLHLRAESVPAHPCGFPAPLPPTRMMESKMIAAIHSS
SADATSSSNYHSFVTASSTSVDDALPLPLPVPQPKHASQKTVYSSFARPDVTTEPFGPD
NCLHFNMTPNCQYRPQSVPPHHNKLEQHQVYGARSEPPASMGLRYNTYVAPGRNASGHH
SKPCSRVEYVSSLSSSVRNTCYPEDIPPYPTIRRVQSLHAPPSSMIRSVPISRTEVPPD
DEPAYCPRPLYQYKPYQSSQARSDYHVTQLQPYFENGRVHYRYSPYSSSSSSYYSPDGA
LCDVDAYGTVQLRPLHRLPNRDFAFYNPRLQGKSLYSYAGLAPRPRANVTGYFSPNDHN
VVSMPPAADVKHTYTSWDLEDMEKYRMQSIRRESRARQKVKGPVMSQYDNMTPAVQDDL
GGIYVIHLRSKSDPGKTGLLSVAEGKESRHAAKAISPEGEDRFYRRHPEAEMDRAHHHG
GHGSTQPEKPSLPQKQSSLRSRKLPDMGCSLPEHRAHQEASHRQFCESKNGPPYPQGAG
QLDYGSKGIPDTSEPVSYHNSGVKYAASGQESLRLNHKEVRLSKEMERPWVRQPSAPEK
HSRDCYKEEEHLTQSIVPPPKPERSHSLKLHHTQNVERDPSVLYQYQPHGKRQSSVTVV
SQYDNLEDYHSLPQHQRGVFGGGGMGTYVPPGFPHPQSRTYATALGQGAFLPAELSLQH
PETQIHAE"
misc_feature 462..467
/label=SDS
misc_feature 534..539
/label=SDS
misc_feature 564..569
/label=SDS
misc_feature 1246..1251
/label=SDS
misc_feature 2310..2315
/label=SDS
misc_feature 2728..2733
/label=SDS
CDS 3093..3158
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3162..3593
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3306..3311
/label=SDS
repeat_region 3610..3750
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3610..3739
/label=AAV2 ITR
repeat_region 3611..3751
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 3825..4280
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4562..4666
/gene="bla"
/label=AmpR promoter
CDS 4667..5527
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5698..6286
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtataact tcgtatagga tactttatag caatttatga
181 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
241 ctgacatcca ctttgccttt ctctccacag ggaatcagcc acatcaagca tatttatctg
301 gggacccaga aaaggccaga attacttcag ttcccttaga ctcagagaag tctgatgatc
361 atgtaagttt ccctgaagac cagtctggga agaacagtat gccaactgtc tccttcttgg
421 atcaggacca gtctccaccc cgtttctaca gtggagatca gcctccttct tatcttggtg
481 caagtgtgga taaactccat caccctttag aatttgcaga caaatctccc acacctccta
541 atttacctag cgataaaatc taccctcctt ctgggtcccc cgaagagaat accagcacag
601 ccaccatgac ttacatgaca actactccag caacagccca aatgagcacc aaggaagcca
661 gctgggatgt ggctgaacaa cccaccactg ctgattttgc tgctgccaca cttcagcgca
721 cgcacagaac taatcgtccc cttccccctc cgccttccca gagatctgca gagcagccac
781 cagttgtggg gcaggtacaa gcagcaacca atataggatt aaataattcc cacaaggttc
841 aaggagtagt tccagttcca gagaggccac ctgaacctcg agccatggat gaccctgcgt
901 ctgccttcat cagtgacagt ggtgctgctg ctgctcagtg tcccatggct acagctgtcc
961 agccaggcct gcctgagaaa gtgcgggacg gtgcccgggt cccgctgctg cacctgcgcg
1021 ccgagtctgt ccctgcgcat ccctgtggct ttcctgcacc actgcccccc accaggatga
1081 tggagagtaa gatgattgct gccatacact ccagcagtgc agatgccacc agcagttcaa
1141 attatcattc ctttgtcact gcttcatcca cctctgtgga cgatgcattg cctttaccac
1201 ttcctgtccc acaacctaag catgcttctc agaaaacagt ttactcctcc tttgctaggc
1261 ccgatgtcac cactgaaccc tttggtccag ataactgttt gcatttcaat atgactccaa
1321 actgccagta ccgtccccag agtgtacctc cccatcacaa taaattggag cagcaccaag
1381 tgtatggtgc caggtcagag ccaccagcct ccatgggtct tcgttataac acatatgtgg
1441 ccccaggaag aaacgcatct ggacaccact ccaagccatg cagccgggtc gagtatgtgt
1501 cttctttgag ctcctctgtc aggaatacct gttaccccga agacattcca ccgtacccta
1561 ccatccggag agtgcagtct ctccatgctc cgccgtcttc catgattcgc tctgttccca
1621 tttcacggac agaagttccc ccagatgatg agccagccta ctgcccaaga cctctgtacc
1681 aatataagcc atatcagtcc tcccaggccc gctcagatta tcatgtcact cagcttcagc
1741 cttactttga gaatggccgg gtccactaca ggtatagccc atattccagt tcttctagtt
1801 cctattacag tccagatggg gccctgtgtg atgtggatgc ctatggcaca gtccagttga
1861 gaccccttca ccgccttccc aatcgagact ttgctttcta caatcctagg ctgcaaggaa
1921 agagcttgta cagttatgct ggtttggctc cacgtccccg ggccaacgtg actggctatt
1981 tctctcccaa cgaccataat gtagtcagca tgcctccggc tgctgatgtg aagcacacct
2041 acacctcatg ggatcttgag gacatggaaa aataccgcat gcagtccatc cggagagaga
2101 gccgtgctcg gcagaaggtg aaagggcctg tcatgtccca atatgataac atgaccccgg
2161 cggtgcagga cgacttgggt gggatctatg tcatccatct gcgtagtaaa tcagatcctg
2221 ggaaaactgg acttctctca gtggcagaag gaaaggagag ccgccatgca gccaaggcca
2281 tcagtcccga gggagaggac cgcttctata ggaggcatcc cgaggcagag atggacagag
2341 cccaccatca cggaggccat ggtagcacgc agccggagaa gccatccctg cctcagaagc
2401 agagcagcct gaggagcagg aagcttcctg acatgggctg cagtcttcct gagcacaggg
2461 cacaccaaga agcaagccat aggcagttct gtgagtcaaa gaatgggccc ccttatcccc
2521 agggagctgg ccagttagat tatgggtcca aagggattcc agacacttct gagccagtca
2581 gctaccacaa ctctggagta aaatatgctg catccgggca agaatcttta agactgaacc
2641 acaaagaggt aaggctctcc aaagagatgg agcgaccctg ggttaggcag ccttctgccc
2701 cagagaaaca ctccagagac tgctacaagg aggaagaaca cctcactcag tcaatcgtcc
2761 caccccctaa accagagagg agtcatagcc tcaaactcca tcatacccag aacgtggaga
2821 gggaccccag tgtgctgtac cagtaccaac cacacggcaa gcgccagagc agtgtgactg
2881 ttgtgtccca gtatgataac ctggaagatt accactccct gcctcagcac cagcgaggag
2941 tctttggagg gggcggcatg gggacgtatg tgccccctgg ctttccccat ccacagagca
3001 ggacctatgc tacagcgttg ggtcaagggg ccttcctgcc cgcagagttg tccttgcagc
3061 atcctgaaac acagatccat gcagaatgta cagactacaa agaccatgac ggtgattata
3121 aagatcatga catcgactac aaggatgacg atgacaagta actcgagata atcaacctct
3181 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
3241 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
3301 tttctcctcc ttgtataaat cctggttagt tcttgccacg gcggaactca tcgccgcctg
3361 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgtttat
3421 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatc tagctttatt tgtgaaattt
3481 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
3541 attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt taacggaccg
3601 agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
3661 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
3721 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc
3781 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg
3841 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
3901 cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
3961 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
4021 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
4081 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
4141 tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat
4201 ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
4261 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
4321 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
4381 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
4441 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
4501 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
4561 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
4621 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
4681 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
4741 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
4801 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
4861 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
4921 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
4981 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
5041 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
5101 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
5161 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
5221 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
5281 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
5341 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
5401 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
5461 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
5521 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
5581 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
5641 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
5701 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
5761 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
5821 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
5881 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
5941 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
6001 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
6061 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
6121 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
6181 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
6241 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
6301 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
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