| 质粒名称: | pBAMD1-4 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 61565 |
| 存储实验室: | Víctor de Lorenzo |
|
载体骨架基本信息 |
|
| 载体名称: | pBAMD1-4 |
| 载体出品公司: | Clontech |
| 空载体大小: | 4730bp |
| 完整质粒大小: | 6156bp |
| 载体类型: | Synthetic Biology ; Mini-Tn5 vector |
| 筛选标记: | Ampicillin |
| 载体抗性: | Streptomycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | Pir1 |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 克隆方法: | Gibson Cloning |
|
文献引用方法 |
|
| 材料和方法部分: | pBAMD1-4 was a gift from Víctor de Lorenzo (Addgene plasmid # 61565 ; http://n2t.net/addgene:61565 ; RRID:Addgene_61565) |
| 参考文献部分: | New transposon tools tailored for metabolic engineering of gram-negative microbial cell factories. Martinez-Garcia E, Aparicio T, de Lorenzo V, Nikel PI. Front Bioeng Biotechnol. 2014 Oct 28;2:46. doi: 10.3389/fbioe.2014.00046. eCollection 2014. 10.3389/fbioe.2014.00046 PubMed 25389526 |
买家导航
pBAMD1-4载体质粒基本信息
pBAMD1-4质粒图谱载体图谱和pBAMD1-4载体序列质粒序列多克隆位点信息
pBAMD1-4质粒载体简介
pBAMD1-4质粒序列载体序列
LOCUS pBAMD1-4 4730 bp DNA circular SYN 14-APR-2025
DEFINITION Standardized mini-Tn5 delivery plasmid, streptomycin/spectinomycin
resistant.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4730)
AUTHORS Martinez-Garcia E, Aparicio T, de Lorenzo V, Nikel PI
TITLE New transposon tools tailored for metabolic engineering of
gram-negative microbial cell factories.
JOURNAL Front Bioeng Biotechnol. 2014 Oct 28;2:46. doi:
10.3389/fbioe.2014.00046. eCollection 2014.
PUBMED 25389526
REFERENCE 2 (bases 1 to 4730)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Front
Bioeng Biotechnol."; date: "2014-10-28"; volume: "2:46. doi"; pages:
" 10.3389/fbioe.2014.00046. eCollection 2014"
COMMENT Sequence Label: pBAMD1-4
FEATURES Location/Qualifiers
source 1..4730
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 10..28
/label=Tn5 ME
/note="hyperactive mosaic end for Tn5 transposase
recognition (Reznikoff et al., 2004)"
misc_feature 62..118
/label=MCS
/note="pUC18/19 multiple cloning site"
CDS 362..1153
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
misc_feature 1246..1264
/label=Tn5 ME
/note="hyperactive mosaic end for Tn5 transposase
recognition (Reznikoff et al., 2004)"
terminator 1275..1369
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
CDS 1474..2901
/codon_start=1
/product="transposase from the bacterial Tn5 transposon
(Reznikoff, 1993)"
/label=Tn5 transposase
/translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKS
ITISSEGSKAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYR
HQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESG
KWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDVESGL
YLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNA
VLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAER
QRMEEPDNLERMVSILSFVAVRLLQLRESFTPPQALRAQGLLKEAEHVESQSAETVLTP
DECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQS
KLDGFLAAKDLMAQGIKI"
promoter 2958..3049
/gene="bla"
/label=AmpR promoter
CDS join(3050..3118,3119..3910)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
terminator 3914..3953
/label=fd terminator
/note="central terminator from bacteriophage fd (Otsuka and
Kunisawa, 1982)"
oriT 4101..4209
/note="incP origin of transfer"
rep_origin 4220..4608
/label=R6K gamma ori
/note="gamma replication origin from E. coli plasmid R6K;
requires the R6K initiator protein pi for replication"
terminator complement(4639..4725)
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
ORIGIN
1 tttaattaac tgtctcttat acacatcttt gtgtctcagg ccgcctaggc cgcggccgcg
61 cgaattcgag ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagcttgc
121 ggccgccaaa gcccgccgaa aggcgggctt ttctgtattt aaatgaacct tgaccgaacg
181 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt
241 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
301 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
361 catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
421 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
481 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
541 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
601 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
661 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
721 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
781 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
841 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
901 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
961 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
1021 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
1081 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt
1141 agtcggcaaa taagacaatt gtctaattaa ttgcggaccc tagaggtccc cttttttatt
1201 ttaaaaattt tttcacaaaa cggtttacaa gcataaaatc tctgaagatg tgtataagag
1261 acagactagt cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga
1321 tttgttcaga acgctcggtt gccgccgggc gttttttatt ggtgagaatc caggggtccc
1381 ctggtttaaa ctacacaagt agcgtcctga acggaacctt tcccgttttc cagaatctga
1441 tgttccatgt gacctcctaa catggtaacg ttcatgatta ccagtgcact gcatcgtgcg
1501 gcggattggg cgaaaagcgt gttttctagt gctgcgctgg gtgatccgcg tcgtaccgcg
1561 cgtctggtga atgttgcggc gcaactggcc aaatatagcg gcaaaagcat taccattagc
1621 agcgaaggca gcaaagccat gcaggaaggc gcgtatcgtt ttattcgtaa tccgaacgtg
1681 agcgcggaag cgattcgtaa agcgggtgcc atgcagaccg tgaaactggc ccaggaattt
1741 ccggaactgc tggcaattga agataccacc tctctgagct atcgtcatca ggtggcggaa
1801 gaactgggca aactgggtag cattcaggat aaaagccgtg gttggtgggt gcatagcgtg
1861 ctgctgctgg aagcgaccac ctttcgtacc gtgggcctgc tgcatcaaga atggtggatg
1921 cgtccggatg atccggcgga tgcggatgaa aaagaaagcg gcaaatggct ggccgctgct
1981 gcaacttcgc gtctgagaat gggcagcatg atgagcaacg tgattgcggt gtgcgatcgt
2041 gaagcggata ttcatgcgta tctgcaagat aaactggccc ataacgaacg ttttgtggtg
2101 cgtagcaaac atccgcgtaa agatgtggaa agcggcctgt atctgtatga tcacctgaaa
2161 aaccagccgg aactgggcgg ctatcagatt agcattccgc agaaaggcgt ggtggataaa
2221 cgtggcaaac gtaaaaaccg tccggcgcgt aaagcgagcc tgagcctgcg tagcggccgt
2281 attaccctga aacagggcaa cattaccctg aacgcggtgc tggccgaaga aattaatccg
2341 ccgaaaggcg aaaccccgct gaaatggctg ctgctgacca gcgagccggt ggaaagtctg
2401 gcccaagcgc tgcgtgtgat tgatatttat acccatcgtt ggcgcattga agaatttcac
2461 aaagcgtgga aaacgggtgc gggtgcggaa cgtcagcgta tggaagaacc ggataacctg
2521 gaacgtatgg tgagcattct gagctttgtg gcggtgcgtc tgctgcaact gcgtgaatct
2581 tttactccgc cgcaagcact gcgtgcgcag ggcctgctga aagaagcgga acacgttgaa
2641 agccagagcg cggaaaccgt gctgaccccg gatgaatgcc aactgctggg ctatctggat
2701 aaaggcaaac gcaaacgcaa agaaaaagcg ggcagcctgc aatgggcgta tatggcgatt
2761 gcgcgtctgg gcggctttat ggatagcaaa cgtaccggca ttgcgagctg gggtgcgctg
2821 tgggaaggtt gggaagcgct gcaaagcaaa ctggatggct ttctggccgc gaaagacctg
2881 atggcgcagg gcattaaaat ctaatcggcg atcgctagta gcccgcctaa tgagcgggct
2941 tttttttaat tcccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
3001 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagcattca
3061 gcattttcgt gtggcgctga ttccgttttt tgcggcgttt tgcctgccgg tgtttgcgca
3121 tccggaaacc ctggtgaaag tgaaagatgc ggaagatcaa ctgggtgcgc gcgtgggcta
3181 tattgaactg gatctgaaca gcggcaaaat tctggaatct tttcgtccgg aagaacgttt
3241 tccgatgatg agcaccttta aagtgctgct gtgcggtgcg gttctgagcc gtgtggatgc
3301 gggccaggaa caactgggcc gtcgtattca ttatagccag aacgatctgg tggaatatag
3361 cccggtgacc gaaaaacatc tgaccgatgg catgaccgtg cgtgaactgt gcagcgcggc
3421 gattaccatg agcgataaca ccgcggcgaa cctgctgctg acgaccattg gcggtccgaa
3481 agaactgacc gcgtttctgc ataacatggg cgatcatgtg acccgtctgg atcgttggga
3541 accggaactg aacgaagcga ttccgaacga tgaacgtgat accaccatgc cggcagcaat
3601 ggcgaccacc ctgcgtaaac tgctgacggg tgagctgctg accctggcaa gccgccagca
3661 actgattgat tggatggaag cggataaagt ggcgggtccg ctgctgcgta gcgcgctgcc
3721 ggctggctgg tttattgcgg ataaaagcgg tgcgggcgaa cgtggcagcc gtggcattat
3781 tgcggcgctg ggcccggatg gtaaaccgag ccgtattgtg gtgatttata ccaccggcag
3841 ccaggcgacg atggatgaac gtaaccgtca gattgcggaa attggcgcga gcctgattaa
3901 acattggtaa accgatacaa ttaaaggctc cttttggagc cttttttttt ggacacgcgt
3961 ttgtcctttt ccgctgcata accctgcttc ggggtcatta tagcgatttt ttcggtatat
4021 ccatcctttt tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga ctttccttgg
4081 tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc gagcgggtgt
4141 tccttcttca ctgtccctta ttcgcacctg gcggtgctca acgggaatcc tgctctgcga
4201 ggctggccgt aggccggccg atctgaagat cagcagttca acctgttgat agtacgtact
4261 aagctctcat gtttcacgta ctaagctctc atgtttaacg tactaagctc tcatgtttaa
4321 cgaactaaac cctcatggct aacgtactaa gctctcatgg ctaacgtact aagctctcat
4381 gtttcacgta ctaagctctc atgtttgaac aataaaatta atataaatca gcaacttaaa
4441 tagcctctaa ggttttaagt tttataagaa aaaaaagaat atataaggct tttaaagcct
4501 ttaaggttta acggttgtgg acaacaagcc agggatgtaa cgcactgaga agcccttaga
4561 gcctctcaaa gcaattttga gtgacacagg aacacttaac ggctgacatg gggcgcgccc
4621 agctgtctag ggcggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
4681 aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc
//
pBAMD1-4载体图谱质粒图谱pdf版和pBAMD1-4载体序列质粒序列等相关资料下载
pBAMD1-4质粒载体应用举例
- 上一篇：AsCas12a-2C-NLS in pCSDest
- 下一篇：pGR475
