pAAVLINK-U6-sgRNA-EF1alpha-5'-CRISPRoff-V2

价格：元

规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg

相关技术服务：质粒构建    基因合成    质粒大提

联系方式：I47-825O-882O

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pAAVLINK-U6-sgRNA-EF1alpha-5'-CRISPRoff-V2载体质粒基本信息

质粒名称: pAAVLINK-U6-sgRNA-EF1alpha-5'-CRISPRoff-V2
出品公司: Wekwikgene
目录编号: 0001904
产品用途: 5' AAVLINK plasmid for CRISPRoff-V2 expression
存储实验室: Zhonghua Lu Lab

载体骨架基本信息
载体名称: pAAV-MCS2
表达系统: 腺病毒
启动子: EF1alpha
拷贝数: 高拷贝
载体抗性: 氨苄霉素
生长温度: 37 ℃
宿主菌: DH5alpha

插入基因信息
插入基因名称: CRISPRoff-V2
物种来源: 未知
密码子优化: 未优化

文献引用方法
参考文献部分:
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review

pAAVLINK-U6-sgRNA-EF1alpha-5'-CRISPRoff-V2质粒图谱载体图谱和pAAVLINK-U6-sgRNA-EF1alpha-5'-CRISPRoff-V2载体序列质粒序列多克隆位点信息

pAAVLINK-U6-sgRNA-EF1alpha-5-CRISPRoff-V2载体图谱

pAAVLINK-U6-sgRNA-EF1alpha-5'-CRISPRoff-V2质粒载体简介

pAAVLINK-U6-sgRNA-EF1alpha-5'-CRISPRoff-V2质粒序列载体序列

LOCUS       pAAVLINK-U6-sgRNA-EF1alpha-5'-CRISPRoff-V2               7599 bp DNA     circular SYN 10-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 15, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7599
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        145..393
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        145..385
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        415..490
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        504..1046
                     /label=EF1-alpha
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
     promoter        533..744
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             757..1025
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    851..856
                     /label=SDS
     CDS             1059..4733
                     /codon_start=1
                     /label=CRISPRoff-V2
                     /translation="MNHDQEFDPPKVYPPVPAEKRKPIRVLSLFDGIATGLLVLKDLGI
                     QVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKHIQEWGPFDLVIGGSPCNDLS
                     IVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFENVVAMGVSDKRDISRFL
                     ESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDKLELQECLEHGRIAKFSKVRT
                     ITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFPVHYTDVSNMSRLARQRLLGR
                     SWSVPVIRHLFAPLKEYFACVSSGNSNANSRGPSFSSGLVPLSLRGSHMGPMEIYKTVS
                     AWKRQPVRVLSLFRNIDKVLKSLGFLESGSGSGGGTLKYVEDVTNVVRRDVEKWGPFDL
                     VYGSTQPLGSSCDRCPGWYMFQFHRILQYALPRQESQRPFFWIFMDNLLLTEDDQETTT
                     RFLQTEAVTLQDVRGRDYQNAMRVWSNIPGLKSKHAPLTPKEEEYLQAQVRSRSKLDAP
                     KVDLLVKNCLLPLREYFKYFSQNSLPLGGPSSGAPPPSGGSPAGSPTSTEEGTSESATP
                     ESGPGTSTEPSEGSAPGSPAGSPTSTEEGTSTEPSEGSAPGTSTEPSEDKKYSIGLAIG
                     TNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRR
                     YTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHE
                     KYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLV
                     QTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGL
                     TPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILR
                     VNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGG
                     ASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRR
                     QEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDK
                     GASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGE
                     QKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIK
                     "
     misc_feature    1436..1441
                     /label=SDS
     misc_feature    1459..1464
                     /label=SDS
     misc_feature    1792..1797
                     /label=SDS
     misc_feature    2618..2623
                     /label=SDS
     CDS             2931..4646
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     intron          4734..4774
                     /label=Splicing Donor
     misc_feature    4784..4817
                     /label=lox2272-JT15
     repeat_region   4862..5002
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4862..4991
                     /label=AAV2 ITR
     repeat_region   4863..5003
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      5077..5532
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5814..5918
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5919..6779
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6950..7538
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgagggc ctatttccca tgattccttc atatttgcat
      181 atacgataca aggctgttag agagataatt ggaattaatt tgactgtaaa cacaaagata
      241 ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa
      301 ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg
      361 gctttatata tcttgtggaa aggacgaaac accggatcta actcaacatg gggggtttta
      421 gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc
      481 gagtcggtgc tttttttgta caggatctgc gatcgctccg gtgcccgtca gtgggcagag
      541 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc
      601 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
      661 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
      721 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc
      781 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc
      841 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga
      901 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt
      961 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac
     1021 agatccaagc tgtgaccggc gcctaccgta cggccaccat gaaccatgac caggaatttg
     1081 accccccaaa ggtttaccca cctgtgccag ctgagaagag gaagcccatc cgcgtgctgt
     1141 ctctctttga tgggattgct acagggctcc tggtgctgaa ggacctgggc atccaagtgg
     1201 accgctacat tgcctccgag gtgtgtgagg actccatcac ggtgggcatg gtgcggcacc
     1261 agggaaagat catgtacgtc ggggacgtcc gcagcgtcac acagaagcat atccaggagt
     1321 ggggcccatt cgacctggtg attggaggca gtccctgcaa tgacctctcc attgtcaacc
     1381 ctgcccgcaa gggactttat gagggtactg gccgcctctt ctttgagttc taccgcctcc
     1441 tgcatgatgc gcggcccaag gagggagatg atcgcccctt cttctggctc tttgagaatg
     1501 tggtggccat gggcgttagt gacaagaggg acatctcgcg atttcttgag tctaaccccg
     1561 tgatgattga cgccaaagaa gtgtctgctg cacacagggc ccgttacttc tggggtaacc
     1621 ttcctggcat gaacaggcct ttggcatcca ctgtgaatga taagctggag ctgcaagagt
     1681 gtctggagca cggcagaata gccaagttca gcaaagtgag gaccattacc accaggtcaa
     1741 actctataaa gcagggcaaa gaccagcatt tccccgtctt catgaacgag aaggaggaca
     1801 tcctgtggtg cactgaaatg gaaagggtgt ttggcttccc cgtccactac acagacgtct
     1861 ccaacatgag ccgcttggcg aggcagagac tgctgggccg atcgtggagc gtgccggtca
     1921 tccgccacct cttcgctccg ctgaaggaat attttgcttg tgtgtctagc ggcaatagta
     1981 acgctaacag ccgcgggccg agcttcagca gcggcctggt gccgttgagc ttgcgcggca
     2041 gccatatggg ccctatggag atatacaaga cagtgtctgc atggaagaga cagccagtgc
     2101 gggtactgag cctcttcaga aacatcgaca aggtactaaa gagtttgggc ttcttggaaa
     2161 gcggttctgg ttctggggga ggaacgctga agtacgtgga agatgtcaca aatgtcgtga
     2221 ggagagacgt ggagaaatgg ggcccctttg acctggtgta cggctcgacg cagcccctag
     2281 gcagctcttg tgatcgctgt cccggctggt acatgttcca gttccaccgc atcctgcagt
     2341 atgcgctgcc tcgccaggag agtcagcggc ccttcttctg gatattcatg gacaatctgc
     2401 tgctgactga ggatgaccaa gagacaacta cccgcttcct tcagacagag gctgtgaccc
     2461 tccaggatgt ccgtggcaga gactaccaga atgctatgcg ggtgtggagc aacattccag
     2521 ggctgaagag caagcatgcg cccctgaccc caaaggaaga agagtatctg caagcccaag
     2581 tcagaagcag gagcaagctg gacgccccga aagttgacct cctggtgaag aactgccttc
     2641 tcccgctgag agagtacttc aagtattttt ctcaaaactc acttcctctt ggagggccga
     2701 gctctggcgc acccccacca agtggagggt ctcctgccgg gtccccaaca tctactgaag
     2761 aaggcaccag cgaatccgca acgcccgagt caggccctgg aacctccaca gaaccatctg
     2821 aaggtagtgc gcctggttcc ccagctggaa gccctacttc caccgaagaa ggcacgtcaa
     2881 ccgaaccaag tgaaggatct gcccctggga ccagcactga accatctgag gacaagaagt
     2941 acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt
     3001 acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga
     3061 agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga
     3121 agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga
     3181 tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct
     3241 tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg
     3301 aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca
     3361 gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc
     3421 ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt
     3481 tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg
     3541 gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc
     3601 tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggcaacctg attgccctga
     3661 gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc
     3721 agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc
     3781 agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca
     3841 tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat
     3901 acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg
     3961 agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc tacattgacg
     4021 gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg
     4081 gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag cagcggacct
     4141 tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc
     4201 ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga
     4261 ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga
     4321 tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg
     4381 gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg
     4441 agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat aacgagctga
     4501 ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga
     4561 aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga
     4621 aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag
     4681 atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggtaagta
     4741 tcaaggttac aagacaggtt taaggagacc aataagcgct agcaattatt cgtataggat
     4801 actttatacg aagttattaa gttatggaga ccggtgtggc acgtgcggac cgagcggccg
     4861 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
     4921 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
     4981 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc
     5041 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
     5101 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc
     5161 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     5221 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     5281 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     5341 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     5401 cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct
     5461 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     5521 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     5581 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     5641 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     5701 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     5761 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     5821 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5881 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5941 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     6001 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     6061 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     6121 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     6181 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     6241 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     6301 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     6361 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6421 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6481 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6541 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6601 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6661 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6721 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6781 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6841 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6901 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6961 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7021 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7081 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7141 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7201 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7261 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7321 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7381 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7441 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7501 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7561 tacggttcct ggccttttgc tggccttttg ctcacatgt
//

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