| 质粒名称: | pAAV-CAG-IFP2-P2A-GFP |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000015 |
| 产品用途: | AAV, Expresses IFP2 and EGFP in cytoplasm of mammalian cells |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 哺乳动物表达 |
| 启动子: |
CAG
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a, TOP10 |
|
插入基因信息 |
|
| 插入基因名称: |
IFP2, EGFP
|
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳动物表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Quantitative assessment of near-infrared fluorescent proteins
Zhang H., Papadaki S., Sun X., Wang X., Drobizhev M., Yao L., Rehbock M., Köster R.W., Wu L., Namikawa K., Piatkevich K.D.
Nat. Methods 2023 2010 20, 1605–1616 (2023)
|
pAAV-CAG-IFP2-P2A-GFP
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAV-CAG-IFP2-P2A-GFP载体质粒基本信息
pAAV-CAG-IFP2-P2A-GFP质粒图谱载体图谱和pAAV-CAG-IFP2-P2A-GFP载体序列质粒序列多克隆位点信息
pAAV-CAG-IFP2-P2A-GFP质粒载体简介
pAAV-CAG-IFP2-P2A-GFP质粒序列载体序列
LOCUS pAAV-CAG-IFP2-P2A-GFP 6496 bp DNA circular SYN 27-MAY-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6496)
AUTHORS Triple Threat
TITLE Direct Submission
JOURNAL Exported Jun 23, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6496
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 279..556
/label=chicken beta-actin promoter
primer_bind 956..1001
/label=1108-IFP2-F
regulatory 977..986
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 983..1945
/codon_start=1
/product="bacteriophytochrome-based monomeric infrared
fluorescent protein (Yu et al., 2014)"
/label=IFP2.0
/translation="MARDPQPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTAD
GHSGEVLQVSLNAATFLGQEPTVLRGQTLAALLPDQWPALQTALPPGCQDALQYRATLD
WPAAGHLSLTVHRVAELLILEFEPTEAWDSIGPHALRNAMFALESAPNLRALAEVATQT
VRELSGFDRVMLYKFAPDATGEVIAEARREGMQAYLGHRFPASTTPAQARALYTRHLLR
LTADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQYLRNMGVGSSLSVSVVVGG
QLWGLIVCHHQTPYVLPPDLRTTLEYLGRLLSLQVQRKEA"
primer_bind complement(1924..1967)
/label=1108-IFP2-R
CDS 1970..2026
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 2039..2755
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
primer_bind complement(2110..2128)
/label=EGFP-R
misc_feature 2780..3368
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(3251..3262)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3411..3532
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(4060..4648)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4819..5679)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5680..5784)
/gene="bla"
/label=AmpR promoter
rep_origin complement(5810..6265)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 tctacgtagc catgctctag gaagagtacc attgacgtca ataatgacgt atgttcccat
61 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
121 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
181 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
241 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
301 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
361 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
421 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
481 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
541 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
601 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
661 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
721 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
781 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
841 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
901 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
961 aattggatcc ggtaccgcca ccatggctcg ggaccctcaa cctttcttcc ctcctctgta
1021 tctcggagga ccagagatca ctactgagaa ctgcgagcgg gaacctatac atataccagg
1081 ttctattcaa cctcatggag cactgctcac agccgatgga cattctggcg aggtgctgca
1141 agtttcactc aacgcagcca ctttcctggg ccaggaacca actgttctgc ggggacagac
1201 cctggcagcc ctgctgccag accagtggcc tgctctgcag actgccctgc ctccaggctg
1261 ccaggacgcc ctgcagtatc gcgccaccct ggattggccc gccgccggac acctgagcct
1321 gaccgtgcat agggtggccg agctgctgat tctggaattc gagcctactg aggcatggga
1381 ctctattgga ccccacgctc tgagaaatgc aatgttcgct ctggagagtg ctcctaatct
1441 ccgggcactg gctgaagtcg caacccaaac agtccgggaa ctgtcaggtt tcgaccgggt
1501 gatgctgtac aagtttgcac cagacgcaac aggagaggtt attgccgaag caaggcgcga
1561 gggcatgcag gcttacctcg ggcataggtt tcccgcatcc accacccctg cacaagctag
1621 ggccctctac acaagacacc tgctccggct gaccgcagac accagggctg cagcagtgcc
1681 cctcgacccc gtgctgaatc cccagacaaa tgctcctaca cctctgggcg gagctgtcct
1741 cagagctaca tccccaatgc acatgcagta cctgaggaat atgggagtgg gctcctccct
1801 gagcgtcagc gtcgtggtcg gcggccagct gtggggactg attgtctgcc accatcagac
1861 accctacgtg ctgccacccg atctgcggac caccctggag tatctgggga ggctcctgtc
1921 cctgcaggtg cagaggaaag aagcccgacc ggttcacgtg ggaagtgggg ctacaaactt
1981 ctcactgctg aaacaggccg gggatgtgga ggaaaaccca ggaccacacg tgactagtgt
2041 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
2101 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
2161 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
2221 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
2281 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
2341 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
2401 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
2461 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
2521 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
2581 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
2641 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
2701 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaagaatt
2761 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
2821 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
2881 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
2941 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
3001 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
3061 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
3121 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
3181 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
3241 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
3301 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
3361 ctccccgcat cgataccgtc gacccgggcg gccgcttcga gcagacatga taagatacat
3421 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
3481 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
3541 caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt tttaaagcaa
3601 gtaaaacctc tacaaatgtg gtaaaatcga taaggatctt cctagagcat ggctacgtag
3661 ataagtagca tggcgggtta atcattaact acaaggaacc cctagtgatg gagttggcca
3721 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
3781 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcatt aatgaatcgg
3841 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
3901 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
3961 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
4021 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
4081 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
4141 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
4201 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
4261 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
4321 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
4381 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
4441 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
4501 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
4561 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
4621 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
4681 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
4741 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
4801 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
4861 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
4921 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
4981 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
5041 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
5101 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
5161 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
5221 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
5281 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
5341 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
5401 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
5461 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
5521 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
5581 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
5641 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
5701 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
5761 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc
5821 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
5881 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
5941 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
6001 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
6061 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
6121 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
6181 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
6241 cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa
6301 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgc gcgctcgctc gctcactgag
6361 gccgcccggg caaagcccgg gcgtcgggcg acctttggtc gcccggcctc agtgagcgag
6421 cgagcgcgca gagagggagt ggccaactcc atcactaggg gttccttgta gttaatgatt
6481 aacccgccat gctact
//
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