| 质粒名称: | pWA21cBP-EGFP-TagBFP |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000447 |
| 产品用途: | Express protein in bacteria |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pWA21cBP |
| 表达系统: | 细菌表达 |
| 启动子: | rhaBAD, araBAD |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | EGFP-AraCDNA, mTagBFP |
| 物种来源: | bacteria |
| 密码子优化: | 人源基因优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Bright and stable monomeric green fluorescent protein derived from StayGold
Zhang H., Lesnov G.D., Subach O.M., Zhang W., Kuzmicheva T.P., Vlaskina A.V., Samygina V.R., Chen L., Ye X., Nikolaeva A.Y., Gabdulkhakov A., Papadaki S., Qin W., Borshchevskiy V., Perfilov M.M., Gavrikov A.S., Drobizhev M., Mishin A.S., Piatkevich K.D., Subach F.V.
Nat. Methods 2024 214 21, 657–665 (2024)
|
买家导航
pWA21cBP-EGFP-TagBFP载体质粒基本信息
pWA21cBP-EGFP-TagBFP质粒图谱载体图谱和pWA21cBP-EGFP-TagBFP载体序列质粒序列多克隆位点信息
pWA21cBP-EGFP-TagBFP质粒载体简介
pWA21cBP-EGFP-TagBFP质粒序列载体序列
LOCUS pWA21cBP-EGFP-TagBFP 7620 bp DNA circular SYN 10-OCT-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7620)
AUTHORS Triple Threat
TITLE Direct Submission
JOURNAL Exported Apr 15, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7620
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 89..349
/gene="cer"
/product="cer"
/label=cer
/note="/vntifkey=21"
CDS 377..392
/gene="pT7"
/product="pT7"
/label=pT7
/note="/vntifkey=4"
promoter 407..525
/label=rhaB promoter
/note="promoter of the E. coli rhaBAD operon, conferring
tight induction with L-rhamnose and repression with
D-glucose in the presence of RhaR and RhaS (Giacalone et
al., 2006)"
primer_bind 439..459
/label=rha-F
RBS 569..573
/label=RBS
/note="/vntifkey=32"
CDS 582..1331
/codon_start=1
/label=EGFP
/note="/vntifkey=21"
/translation="MTMITHHHHHHGCSKGEELFTGVVPILVELDGDVNGHKFSVYGEG
EGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGY
VQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVY
IMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKD
PNEKRDHMVLLEFVTAAGITLGMDELYK"
primer_bind complement(662..688)
/label=EBFP2-Rseq
primer_bind complement(689..705)
/label=EGFP-R
primer_bind 1157..1175
/label=WKG-423-F1
primer_bind complement(1192..1212)
/label=EGFP-Rseq2
primer_bind 1280..1300
/label=EGFP-F
misc_feature 1332..1733
/label=araC DNA binding domain
/label=araC\DNA\binding\domain
/note="/vntifkey=21"
misc_feature 1383..1421
/label=helix1
misc_feature 1443..1463
/label=helix2
misc_feature 1476..1511
/label=helix3
misc_feature 1521..1577
/label=helix4
misc_feature 1590..1610
/label=helix5
misc_feature 1626..1661
/label=helix6
misc_feature 1671..1688
/label=helix7
terminator 1822..1979
/label=rrnB T1 and T2
/label=rrnB\T1\and\T2
/note="/vntifkey=43"
primer_bind complement(1956..1974)
/label=rrnbt1-Rseq2
primer_bind 1989..2011
/label=RRNBT1-R
promoter 2436..2708
/label=araBAD promoter
/label=araBAD\promoter
/note="/vntifkey=30"
primer_bind 2595..2621
/label=ARABAD-F
primer_bind complement(2730..2754)
/label=WKG-447-R
RBS 2737..2741
/label=RBS
/note="/vntifkey=32"
CDS 2751..3554
/codon_start=1
/label=TagBFP
/note="/vntifkey=21"
/translation="MGGSHHHHHHGMASMTGGQQMGRDLYDDDDKDPSSSMSELIKENM
HMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVVEGGPLPFAFDILATSFLYGSKT
FINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFT
SNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLVGGSHLIANIKTTYRSKKPAKN
LKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCDLPSKLGHK"
terminator 3662..3819
/label=rrnB T1 and T2
/label=rrnB\T1\and\T2
/note="/vntifkey=43"
primer_bind complement(3796..3814)
/label=rrnbt1-Rseq2
primer_bind 3829..3851
/label=RRNBT1-R
misc_feature 4017..4115
/label=Bla promoter
/label=Bla\promoter
/note="/vntifkey=21"
CDS 4116..4976
/gene="bla"
/product="bla"
/label=bla
/note="/vntifkey=4"
misc_feature 5123..5795
/label=pBR322 origin
/label=pBR322\origin
/note="/vntifkey=21"
misc_feature 5771
/gene="ori"
/product="ori"
/label=ori
/note="/vntifkey=21"
primer_bind 5814..5840
/label=WKG-304-F1
CDS complement(6165..6356)
/gene="rop"
/label=rop
/note="/vntifkey=4"
ORIGIN
1 gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga
61 gcaccgccgc cgcaaggaat ggtgcatgct cgatggctac gagggcagac agtaagtgga
121 tttaccataa tcccttaatt gtacgcaccg ctaaaacgcg ttcagcgcga tcacggcagc
181 agacaggtaa aaatggcaac aaaccacccg aaaaactgcc gcgatcgcgc ctgataaatt
241 ttaaccgtat gaatacctat gcaaccagag ggtacaggcc acattacccc cacttaatcc
301 actgaagctg ccatttttca tggtttcacc atcccagcga agggccatgc atgcatcgaa
361 attaatacga cgaaattaat acgactcact atagggcaat tgcgatcacc acaattcagc
421 aaattgtgaa catcatcacg ttcatctttc cctggttgcc aatggcccat tttcctgtca
481 gtaacgagaa ggtcgcgaat tcaggcgctt tttagactgg tcgtagggag accacaacgg
541 tttccctcta gaaataattt tcttaagaag gagatataca tatgaccatg attacgcatc
601 atcatcatca tcatggctgc agcaagggcg aggagctgtt caccggggtg gtgcccatcc
661 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtacggc gagggcgagg
721 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
781 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
841 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
901 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
961 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
1021 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
1081 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
1141 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
1201 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
1261 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
1321 agctgtacaa gatctcaggt tcaatggaag cgattaacga gtcgctccat ccaccgatgg
1381 ataatcgggt acgcgaggct tgtcagtaca tcagcgatca cctggcagac agcaattttg
1441 atatcgccag cgtcgcacag catgtttgct tgtcgccgtc gcgtctgtca catcttttcc
1501 gccagcagtt agggattagc gtcttaagct ggcgcgagga ccaacgtatc agccaggcga
1561 agctgctttt gagcaccacc cggatgccta tcgccaccgt cggtcgcaat gttggttttg
1621 acgatcaact ctatttctcg cgggtattta aaaaatgcac cggggccagc ccgagcgagt
1681 tccgtgccgg ttgtgaagaa aaagtgaatg atgtagccgt caagttgtca taaaagcttg
1741 gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa
1801 gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca
1861 tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga
1921 gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
1981 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg
2041 gatttgacct aggcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
2101 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
2161 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
2221 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagca
2281 gatcaattcg cgcgcgaagg cgaagcggca tgcataatgt gcctgtcaaa tggacgaagc
2341 agggattctg caaaccctat gctactccgt caagccgtca attgtctgat tcgttaccaa
2401 ttacatactt ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag
2461 acattgccgt cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct
2521 tattaaaagc attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag
2581 tgtctataat cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta
2641 tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact
2701 ctctactgtt tctccatacc cgttttttgg gctaacagga ggaattaacc atggggggtt
2761 ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa atgggtcggg
2821 atctgtacga cgatgacgat aaggatccga gctcgagtat gagcgagctg atcaaggaga
2881 acatgcacat gaagctgtac atggagggca ccgtggacaa ccatcacttc aagtgcacat
2941 ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg
3001 gcggccctct ccccttcgcc ttcgacatcc tggctactag cttcctctac ggcagcaaga
3061 ccttcatcaa ccacacccag ggcatccccg acttcttcaa gcagtccttc cctgagggct
3121 tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca
3181 ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcacat
3241 ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccttcacc gagacgctgt
3301 accccgctga cggcggcctg gaaggcagaa acgacatggc cctgaagctc gtgggcggga
3361 gccatctgat cgcaaacatc aagaccacat atagatccaa gaaacccgct aagaacctca
3421 agatgcctgg cgtctactat gtggactaca gactggaaag aatcaaggag gccaacaacg
3481 agacctacgt cgagcagcac gaggtggcag tggccagata ctgcgacctc cctagcaaac
3541 tggggcacaa gtaaggtacc atatgggaat tcgaagcttg gctgttttgg cggatgagag
3601 aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat aaaacagaat
3661 ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc agaagtgaaa
3721 cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa ctgccaggca
3781 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
3841 ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca
3901 acggcccgga gggtggcggc ggccgcgagg gtggcgggca ggacgcccgc cataaactgc
3961 caggcatcaa attaagcaga aggccatcct gacggatggc ctttttgcgt ttctacaaac
4021 tcttttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
4081 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
4141 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
4201 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
4261 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
4321 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
4381 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
4441 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
4501 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
4561 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
4621 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
4681 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
4741 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
4801 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
4861 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
4921 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
4981 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
5041 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
5101 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
5161 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
5221 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
5281 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
5341 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
5401 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
5461 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
5521 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
5581 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
5641 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
5701 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
5761 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
5821 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
5881 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
5941 ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc
6001 tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct
6061 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
6121 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
6181 tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat
6241 tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat
6301 gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat
6361 gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg
6421 atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt
6481 aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag
6541 cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag
6601 atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg
6661 aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt
6721 cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct
6781 agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg
6841 cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa
6901 gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg
6961 gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg
7021 caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca
7081 acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccaatga
7141 tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat
7201 ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa
7261 gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca
7321 gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg
7381 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc
7441 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg
7501 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca
7561 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac
//
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