| 出品公司: | Addgene |
|---|---|
| 载体名称: | PX399 或 PX399 Mycoplasma gallisepticum str. F Cas9 |
| 质粒类型: | CRISPR-Cas9载体；基因敲除载体；昆虫细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 插入位点: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 11003 bp |
| 5' 测序引物及序列: | CMV Forward:CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | -- |
| 载体标签: | NLS (C ter)；HA、NLS (N ter) |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Neomycin |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | 昆虫细胞 |
| 备注: | -- |
| 产品目录号: | #68335 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 杆状病毒 |
买家导航
PX399载体质粒基本信息
PX399质粒图谱载体图谱和PX399载体序列质粒序列多克隆位点信息
PX399质粒载体简介
PX399质粒序列载体序列
LOCUS PX399 Mycoplasma gallisepticum str. F Cas9 11003 bp DNA SYN
DEFINITION PX399 Mycoplasma gallisepticum str. F Cas9
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..11003
/organism="PX399 Mycoplasma gallisepticum str. F Cas9"
/mol_type="other DNA"
misc_feature 47..1001
/label="Ac_5_flank"
CDS complement(343..948)
/label="ORF frame 3"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
promoter 1023..1587
/label="CMV_immearly_promoter"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
misc_feature 1090..1377
/label="CAG_enhancer"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
misc_feature 1544..1564
/label="CMV_fwd_primer"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
promoter 1615..1633
/label="T7_promoter"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
misc_feature 1633..1656
/label="lacO"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
misc_feature 1772..1796
/label="attB1"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
CDS 1822..5694
/label="ORF frame 1"
/translation="MYPYDVPDYASPKKKRKVEASMNNSIKSKPEVTIGLDLGVGSVG
WAIVDNETNIIHHLGSRLFSQAKTAEDRRSFRGVRRLIRRRKYKLKRFVNLIWKYNSY
FGFKNKEDILNNYQEQQKLHNTVLNLKSEALNAKIDPKALSWILHDYLKNRGHFYEDN
RDFNVYPTKELAKYFDKYGYYKGIIDSKEDNDNKLEEELTKYKFSNKHWLEEVKKVLS
NQTGLPEKFKEEYESLFSYVRNYSEGPGSINSVSPYGIYHLDEKEGKVVQKYNNIWDK
TIGKCNIFPDEYRAPKNSPIAMIFNEINELSTIRSYSIYLTGWFINQEFKKAYLNKLL
DLLIKTNGEKPIDARQFKKLREETIAESIGKETLKDVENEEKLEKEDHKWKLKGLKLN
TNGKIQYNDLSSLAKFVHKLKQHLKLDFLLEDQYATLDKINFLQSLFVYLGKHLRYSN
RVDSANLKEFSDSNKLFERILQKQKDGLFKLFEQTDKDDEKILAQTHSLSTKAMLLAI
TRMTNLDNDEDNQKNNDKGWNFEAIKNFDQKFIDITKKNNNLSLKQNKRYLDDRFIND
AILSPGVKRILREATKVFNAILKQFSEEYDVTKVVIELARELSEEKELENTKNYKKLI
KKNGDKISEGLKALGISEDEIKDILKSPTKSYKFLLWLQQDHIDPYSLKEIAFDDIFT
KTEKFEIDHIIPYSISFDDSSSNKLLVLAESNQAKSNQTPYEFISSGNAGIKWEDYEA
YCRKFKDGDSSLLDSTQRSKKFAKMMKTDTSSKYDIGFLARNLNDTRYATIVFRDALE
DYANNHLVEDKPMFKVVCINGSVTSFLRKNFDDSSYAKKDRDKNIHHAVDASIISIFS
NETKTLFNQLTQFADYKLFKNTDGSWKKIDPKTGVVTEVTDENWKQIRVRNQVSEIAK
VIEKYIQDSNIERKARYSRKIENKTNISLFNDTVYSAKKVGYEDQIKRKNLKTLDIHE
SAKENKNSKVKRQFVYRKLVNVSLLNNDKLADLFAEKEDILMYRANPWVINLAEQIFN
EYTENKKIKSQNVFEKYMLDLTKEFPEKFSEFLVKSMLRNKTAIIYDDKKNIVHRIKR
LKMLSSELKENKLSNVIIRSKNQSGTKLSYQDTINSLALMIMRSIDPTAKKQYIRVPL
NTLNLHLGDHDFDLHNMDAYLKKPKFVKYLKANEIGDEYKPWRVLTSGTLLIHKKDKK
LMYISSFQNLNDVIEIKNLIETEYKENDDSDSKKKKKANRFLMTLSTILNDYILLDAK
DNFDILGLSKNRIDEILNSKLGLDKIVK*"
misc_feature 1825..1851
/label="HA"
/translation="MYPYDVPDYASPKKKRKVEASMNNSIKSKPEVTIGLDLGVGSVG
WAIVDNETNIIHHLGSRLFSQAKTAEDRRSFRGVRRLIRRRKYKLKRFVNLIWKYNSY
FGFKNKEDILNNYQEQQKLHNTVLNLKSEALNAKIDPKALSWILHDYLKNRGHFYEDN
RDFNVYPTKELAKYFDKYGYYKGIIDSKEDNDNKLEEELTKYKFSNKHWLEEVKKVLS
NQTGLPEKFKEEYESLFSYVRNYSEGPGSINSVSPYGIYHLDEKEGKVVQKYNNIWDK
TIGKCNIFPDEYRAPKNSPIAMIFNEINELSTIRSYSIYLTGWFINQEFKKAYLNKLL
DLLIKTNGEKPIDARQFKKLREETIAESIGKETLKDVENEEKLEKEDHKWKLKGLKLN
TNGKIQYNDLSSLAKFVHKLKQHLKLDFLLEDQYATLDKINFLQSLFVYLGKHLRYSN
RVDSANLKEFSDSNKLFERILQKQKDGLFKLFEQTDKDDEKILAQTHSLSTKAMLLAI
TRMTNLDNDEDNQKNNDKGWNFEAIKNFDQKFIDITKKNNNLSLKQNKRYLDDRFIND
AILSPGVKRILREATKVFNAILKQFSEEYDVTKVVIELARELSEEKELENTKNYKKLI
KKNGDKISEGLKALGISEDEIKDILKSPTKSYKFLLWLQQDHIDPYSLKEIAFDDIFT
KTEKFEIDHIIPYSISFDDSSSNKLLVLAESNQAKSNQTPYEFISSGNAGIKWEDYEA
YCRKFKDGDSSLLDSTQRSKKFAKMMKTDTSSKYDIGFLARNLNDTRYATIVFRDALE
DYANNHLVEDKPMFKVVCINGSVTSFLRKNFDDSSYAKKDRDKNIHHAVDASIISIFS
NETKTLFNQLTQFADYKLFKNTDGSWKKIDPKTGVVTEVTDENWKQIRVRNQVSEIAK
VIEKYIQDSNIERKARYSRKIENKTNISLFNDTVYSAKKVGYEDQIKRKNLKTLDIHE
SAKENKNSKVKRQFVYRKLVNVSLLNNDKLADLFAEKEDILMYRANPWVINLAEQIFN
EYTENKKIKSQNVFEKYMLDLTKEFPEKFSEFLVKSMLRNKTAIIYDDKKNIVHRIKR
LKMLSSELKENKLSNVIIRSKNQSGTKLSYQDTINSLALMIMRSIDPTAKKQYIRVPL
NTLNLHLGDHDFDLHNMDAYLKKPKFVKYLKANEIGDEYKPWRVLTSGTLLIHKKDKK
LMYISSFQNLNDVIEIKNLIETEYKENDDSDSKKKKKANRFLMTLSTILNDYILLDAK
DNFDILGLSKNRIDEILNSKLGLDKIVK*"
misc_feature 1855..1875
/label="SV40 NLS"
/translation="MYPYDVPDYASPKKKRKVEASMNNSIKSKPEVTIGLDLGVGSVG
WAIVDNETNIIHHLGSRLFSQAKTAEDRRSFRGVRRLIRRRKYKLKRFVNLIWKYNSY
FGFKNKEDILNNYQEQQKLHNTVLNLKSEALNAKIDPKALSWILHDYLKNRGHFYEDN
RDFNVYPTKELAKYFDKYGYYKGIIDSKEDNDNKLEEELTKYKFSNKHWLEEVKKVLS
NQTGLPEKFKEEYESLFSYVRNYSEGPGSINSVSPYGIYHLDEKEGKVVQKYNNIWDK
TIGKCNIFPDEYRAPKNSPIAMIFNEINELSTIRSYSIYLTGWFINQEFKKAYLNKLL
DLLIKTNGEKPIDARQFKKLREETIAESIGKETLKDVENEEKLEKEDHKWKLKGLKLN
TNGKIQYNDLSSLAKFVHKLKQHLKLDFLLEDQYATLDKINFLQSLFVYLGKHLRYSN
RVDSANLKEFSDSNKLFERILQKQKDGLFKLFEQTDKDDEKILAQTHSLSTKAMLLAI
TRMTNLDNDEDNQKNNDKGWNFEAIKNFDQKFIDITKKNNNLSLKQNKRYLDDRFIND
AILSPGVKRILREATKVFNAILKQFSEEYDVTKVVIELARELSEEKELENTKNYKKLI
KKNGDKISEGLKALGISEDEIKDILKSPTKSYKFLLWLQQDHIDPYSLKEIAFDDIFT
KTEKFEIDHIIPYSISFDDSSSNKLLVLAESNQAKSNQTPYEFISSGNAGIKWEDYEA
YCRKFKDGDSSLLDSTQRSKKFAKMMKTDTSSKYDIGFLARNLNDTRYATIVFRDALE
DYANNHLVEDKPMFKVVCINGSVTSFLRKNFDDSSYAKKDRDKNIHHAVDASIISIFS
NETKTLFNQLTQFADYKLFKNTDGSWKKIDPKTGVVTEVTDENWKQIRVRNQVSEIAK
VIEKYIQDSNIERKARYSRKIENKTNISLFNDTVYSAKKVGYEDQIKRKNLKTLDIHE
SAKENKNSKVKRQFVYRKLVNVSLLNNDKLADLFAEKEDILMYRANPWVINLAEQIFN
EYTENKKIKSQNVFEKYMLDLTKEFPEKFSEFLVKSMLRNKTAIIYDDKKNIVHRIKR
LKMLSSELKENKLSNVIIRSKNQSGTKLSYQDTINSLALMIMRSIDPTAKKQYIRVPL
NTLNLHLGDHDFDLHNMDAYLKKPKFVKYLKANEIGDEYKPWRVLTSGTLLIHKKDKK
LMYISSFQNLNDVIEIKNLIETEYKENDDSDSKKKKKANRFLMTLSTILNDYILLDAK
DNFDILGLSKNRIDEILNSKLGLDKIVK*"
misc_feature 1861..1875
/label="NLS"
/translation="MYPYDVPDYASPKKKRKVEASMNNSIKSKPEVTIGLDLGVGSVG
WAIVDNETNIIHHLGSRLFSQAKTAEDRRSFRGVRRLIRRRKYKLKRFVNLIWKYNSY
FGFKNKEDILNNYQEQQKLHNTVLNLKSEALNAKIDPKALSWILHDYLKNRGHFYEDN
RDFNVYPTKELAKYFDKYGYYKGIIDSKEDNDNKLEEELTKYKFSNKHWLEEVKKVLS
NQTGLPEKFKEEYESLFSYVRNYSEGPGSINSVSPYGIYHLDEKEGKVVQKYNNIWDK
TIGKCNIFPDEYRAPKNSPIAMIFNEINELSTIRSYSIYLTGWFINQEFKKAYLNKLL
DLLIKTNGEKPIDARQFKKLREETIAESIGKETLKDVENEEKLEKEDHKWKLKGLKLN
TNGKIQYNDLSSLAKFVHKLKQHLKLDFLLEDQYATLDKINFLQSLFVYLGKHLRYSN
RVDSANLKEFSDSNKLFERILQKQKDGLFKLFEQTDKDDEKILAQTHSLSTKAMLLAI
TRMTNLDNDEDNQKNNDKGWNFEAIKNFDQKFIDITKKNNNLSLKQNKRYLDDRFIND
AILSPGVKRILREATKVFNAILKQFSEEYDVTKVVIELARELSEEKELENTKNYKKLI
KKNGDKISEGLKALGISEDEIKDILKSPTKSYKFLLWLQQDHIDPYSLKEIAFDDIFT
KTEKFEIDHIIPYSISFDDSSSNKLLVLAESNQAKSNQTPYEFISSGNAGIKWEDYEA
YCRKFKDGDSSLLDSTQRSKKFAKMMKTDTSSKYDIGFLARNLNDTRYATIVFRDALE
DYANNHLVEDKPMFKVVCINGSVTSFLRKNFDDSSYAKKDRDKNIHHAVDASIISIFS
NETKTLFNQLTQFADYKLFKNTDGSWKKIDPKTGVVTEVTDENWKQIRVRNQVSEIAK
VIEKYIQDSNIERKARYSRKIENKTNISLFNDTVYSAKKVGYEDQIKRKNLKTLDIHE
SAKENKNSKVKRQFVYRKLVNVSLLNNDKLADLFAEKEDILMYRANPWVINLAEQIFN
EYTENKKIKSQNVFEKYMLDLTKEFPEKFSEFLVKSMLRNKTAIIYDDKKNIVHRIKR
LKMLSSELKENKLSNVIIRSKNQSGTKLSYQDTINSLALMIMRSIDPTAKKQYIRVPL
NTLNLHLGDHDFDLHNMDAYLKKPKFVKYLKANEIGDEYKPWRVLTSGTLLIHKKDKK
LMYISSFQNLNDVIEIKNLIETEYKENDDSDSKKKKKANRFLMTLSTILNDYILLDAK
DNFDILGLSKNRIDEILNSKLGLDKIVK*"
CDS complement(2752..3312)
/label="ORF frame 3"
/translation="MCLGQDLLIVFVSLFKQLEQPILLFLQDALEQFVRVGKFLQVGR
IDPIGISQVLAQVHKQGLQEIDLVQCCILVLQQEIQFQMLLQFVHKLSQGAQIVVLDF
AVGIQLQSLQFPLVVLLFQFLFILHILQCLFANAFGDCLFAQFLELPCVNWLLSVGFD
QQIQQLVQVGFLELLINEPASQVDAV*"
CDS complement(2805..3419)
/label="ORF frame 1"
/translation="MASKFQPLSLFFWLSSSLSRFVIRVIASSMALVDRLCVWARIFS
SSLSVCSNSLNSPSFCFCRMRSNSLLESENSFRLAESTLLEYLRCLPRYTNRDCRKLI
LSSVAYWSSSRKSSFRCCFSLCTNLARELRSLYWILPLVFSFSPFSFHLWSSFSSFSS
FSTSFSVSLPMLSAIVSSRSFLNCLASIGFSPLVLISRSSSLFR*"
CDS complement(4378..5073)
/label="ORF frame 3"
/translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
QLVEQSLCLVAEN*"
misc_feature complement(5727..5744)
/label="Sp6_primer"
/translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
QLVEQSLCLVAEN*"
misc_feature 5749..5773
/label="attB2"
/translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
QLVEQSLCLVAEN*"
terminator 5785..6251
/label="hGH_PA_terminator"
/translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
QLVEQSLCLVAEN*"
terminator 6257..6292
/label="T7_terminator"
/translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
QLVEQSLCLVAEN*"
rep_origin 6409..6715
/label="f1_origin"
/translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
QLVEQSLCLVAEN*"
misc_feature complement(6829..6849)
/label="pBABE_3_primer"
/translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
QLVEQSLCLVAEN*"
misc_feature complement(6835..7050)
/label="SV40_enhancer"
/translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
QLVEQSLCLVAEN*"
promoter 6847..7115
/label="SV40_promoter"
/translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
QLVEQSLCLVAEN*"
rep_origin 7014..7091
/label="SV40_origin"
/translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
QLVEQSLCLVAEN*"
misc_feature 7076..7095
/label="SV40pro_F_primer"
/translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
QLVEQSLCLVAEN*"
CDS 7230..8024
/label="ORF frame 3"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
gene 7233..8021
/label="NeoR/KanR"
/gene="NeoR/KanR"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
CDS complement(7539..8075)
/label="ORF frame 1"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
terminator 8201..8320
/label="SV40_PA_terminator"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
misc_feature 8289..8308
/label="EBV_rev_primer"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
terminator 8511..8546
/label="T7_terminator"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
misc_feature complement(8569..8586)
/label="Baculovirs_rev_primer"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
misc_feature 8576..8653
/label="3_AcPH"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
CDS complement(8683..9300)
/label="ORF frame 3"
/translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
misc_feature 9280..9313
/label="loxP"
/translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
gene complement(10081..10941)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
CDS complement(10081..10941)
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
1861 aaaaagcgca aggtcgaagc gtccatgaac aatagcatca aatctaaacc tgaagtgacc
1921 atcgggctgg acctgggagt gggaagcgtg gggtgggcaa tcgtggataa cgaaacaaac
1981 atcattcacc atctgggctc caggctgttt tctcaggcca agactgctga ggatcggaga
2041 tctttccgcg gggtgaggcg cctgatccga cggagaaaat acaagctgaa acgattcgtc
2101 aatctgattt ggaagtacaa cagctatttc ggcttcaaga acaaagagga catcctgaac
2161 aattatcagg agcagcagaa gctgcacaat accgtgctga acctgaaatc agaggcactg
2221 aatgccaaga tcgatcctaa agcactgagc tggattctgc acgactacct gaagaacaga
2281 ggccattttt atgaggacaa tagggatttc aacgtgtacc caacaaagga gctggccaag
2341 tacttcgata agtacgggta ctacaaggga atcattgaca gcaaggagga caatgataac
2401 aaactggagg aagagctgac aaagtacaaa ttctccaata agcactggct ggaagaggtg
2461 aagaaagtcc tgtctaacca gactggcctg ccagaaaagt ttaaagaaga gtatgagtca
2521 ctgttcagct acgtgagaaa ttattcagag ggcccaggga gcatcaactc tgtcagtccc
2581 tacgggatct accatctgga cgaaaaagag ggaaaggtgg tccagaagta caacaacatc
2641 tgggataaga caatcggaaa gtgcaacatc ttccctgacg agtatagagc tcccaagaac
2701 agtcctatcg caatgatttt caatgaaatc aacgagctgt ccacaatcag gtcatacagc
2761 atctacctga ctggctggtt cattaatcag gagttcaaga aagcctacct gaacaagctg
2821 ctggatctgc tgatcaaaac caacggagag aagccaattg acgcaaggca gttcaagaaa
2881 ctgcgcgaag agacaatcgc cgaaagcatt ggcaaagaga cactgaagga tgtggagaat
2941 gaagagaaac tggaaaagga ggaccacaag tggaaactga agggactgaa gctgaatacc
3001 aacggcaaaa tccagtacaa cgatctgagc tccctggcta agtttgtgca caaactgaag
3061 cagcatctga aactggattt cctgctggag gaccagtatg caacactgga caagatcaat
3121 ttcctgcagt ccctgtttgt gtacctgggc aagcacctga gatattccaa tagggtcgat
3181 tctgccaacc tgaaggaatt ttccgactct aacaaactgt tcgagcgcat cctgcagaaa
3241 cagaaggatg ggctgttcaa gctgtttgaa cagactgaca aagacgatga gaagatcctg
3301 gcccagacac atagtctgtc aactaaggcc atgctgctgg ctattacccg gatgacaaat
3361 ctggacaacg atgaggacaa ccagaaaaac aatgacaagg gctggaattt tgaggccatc
3421 aaaaacttcg atcagaagtt tatcgacatc accaagaaaa acaacaacct gagcctgaaa
3481 cagaataagc gctacctgga cgatcgattc atcaacgatg ctattctgtc ccctggggtg
3541 aagcgaatcc tgcgggaggc aaccaaggtc tttaatgcca ttctgaaaca gttctctgaa
3601 gagtacgacg tgacaaaggt ggtcatcgaa ctggctcgcg agctgagcga agagaaggaa
3661 ctggagaaca caaagaacta caagaaactg atcaagaaaa acggcgacaa gattagtgag
3721 ggcctgaaag cactggggat ctcagaagat gagatcaaag acattctgaa gagtcccact
3781 aaatcataca agtttctgct gtggctgcag caggaccaca tcgatcctta tagcctgaag
3841 gagatcgcct tcgacgatat ttttaccaaa acagaaaagt tcgagatcga ccatatcatt
3901 ccctacagca tttccttcga cgattctagt tcaaacaagc tgctggtgct ggctgaaagt
3961 aatcaggcaa agtcaaacca gactccttat gagttcatca gctccggaaa cgcaggcatt
4021 aagtgggaag attacgaggc ctattgccgc aagttcaagg atggggactc tagtctgctg
4081 gacagcaccc agcggtccaa gaaattcgcc aaaatgatga aaaccgatac ctcaagcaag
4141 tacgacatcg gatttctggc tcgaaatctg aacgatactc ggtacgcaac cattgtgttc
4201 cgggacgccc tggaggacta tgctaataac cacctggtcg aggacaaacc catgtttaag
4261 gtggtctgta tcaatgggtc cgtgacctct ttcctgcgga agaactttga cgattcctct
4321 tacgccaaga aagatagaga caagaatatc caccatgctg tggatgcaag tatcatctca
4381 attttcagca acgagacaaa gactctgttc aaccagctga ctcagtttgc tgactataaa
4441 ctgttcaaga acaccgatgg cagctggaag aaaatcgacc ctaagacagg ggtggtcact
4501 gaagtgaccg acgagaattg gaagcagatt agggtgcgca accaggtgag cgaaatcgcc
4561 aaagtcattg agaagtacat ccaggatagc aacatcgaaa gaaaggctag gtattcccgc
4621 aaaatcgaga ataagactaa catttccctg tttaatgaca ccgtgtactc tgccaagaaa
4681 gtcggctatg aggatcagat caaaagaaag aacctgaaaa ccctggacat tcacgaatct
4741 gctaaagaga ataagaacag taaagtgaag cggcagtttg tctacagaaa gctggtgaat
4801 gtcagcctgc tgaataacga taagctggca gacctgttcg ccgaaaaaga ggatatcctg
4861 atgtataggg ccaatccatg ggtcatcaac ctggctgagc agattttcaa tgaatacact
4921 gagaacaaga aaatcaagtc ccagaacgtg tttgaaaaat atatgctgga cctgaccaaa
4981 gagttccccg agaagttcag cgagtttctg gtgaagtcca tgctgagaaa caagaccgcc
5041 atcatctacg acgataagaa aaacattgtc catcgaatca aacggctgaa gatgctgagt
5101 tcagaactga aagagaataa gctgtctaac gtgatcatta ggtctaagaa tcagagtggg
5161 accaaactgt cataccagga tacaatcaac agcctggccc tgatgattat gcgcagcatc
5221 gaccctactg ctaagaaaca gtatattcga gtgccactga ataccctgaa cctgcacctg
5281 ggagatcatg actttgatct gcacaatatg gatgcttacc tgaagaaacc aaaattcgtg
5341 aagtatctga aagcaaacga aatcggcgac gagtacaagc cctggagggt cctgacatct
5401 ggcactctgc tgatccataa gaaggataag aaactgatgt acatcagctc cttccagaat
5461 ctgaacgacg tgatcgaaat taagaatctg atcgaaaccg agtataaaga gaacgacgat
5521 tctgatagta agaaaaagaa aaaggcaaac cgctttctga tgaccctgag cacaatcctg
5581 aatgactaca ttctgctgga cgccaaggat aacttcgaca tcctggggct gtctaaaaat
5641 cggatcgatg agattctgaa cagtaagctg ggactggaca agattgtgaa ataagaattc
5701 ggtaccgagc tcaagcttgc ggccgcctat agtgtcacct aaatcgtaac ccagctttct
5761 tgtacaaagt ggtcgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg
5821 aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt
5881 ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc
5941 aagttgggat gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag
6001 tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc
6061 agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt
6121 tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg
6181 tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct
6241 tccctgtcct tctagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgt
6301 ctagaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
6361 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
6421 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
6481 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
6541 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
6601 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
6661 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
6721 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
6781 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
6841 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
6901 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
6961 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
7021 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
7081 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
7141 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
7201 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
7261 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
7321 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
7381 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
7441 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
7501 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
7561 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
7621 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
7681 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
7741 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
7801 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
7861 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
7921 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
7981 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
8041 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
8101 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
8161 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
8221 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
8281 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgtt catgaagccc
8341 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg
8401 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag
8461 aatgagtatt tggtttagag tttggcaaca tatgcccata tgtaactagc ataacccctt
8521 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag catgcggagg aaattctcct
8581 tgaagtttcc ctggtgttca aagtaaagga gtttgcacca gacgcacctc tgttcactgg
8641 tccggcgtat taaaacacga tacattgtta ttagtacatt tattaagcgc tagattctgt
8701 gcgttgttga tttacagaca attgttgtac gtattttaat aattcattaa atttataatc
8761 tttagggtgg tatgttagag cgaaaatcaa atgattttca gcgtctttat atctgaattt
8821 aaatattaaa tcctcaatag atttgtaaaa taggtttcga ttagtttcaa acaagggttg
8881 tttttccgaa ccgatggctg gactatctaa tggattttcg ctcaacgcca caaaacttgc
8941 caaatcttgt agcagcaatc tagctttgtc gatattcgtt tgtgttttgt tttgtaataa
9001 aggttcgacg tcgttcaaaa tattatgcgc ttttgtattt ctttcatcac tgtcgttagt
9061 gtacaattga ctcgacgtaa acacgttaaa tagagcttgg acatatttaa catcgggcgt
9121 gttagcttta ttaggccgat tatcgtcgtc gtcccaaccc tcgtcgttag aagttgcttc
9181 cgaagacgat tttgccatag ccacacgacg cctattaatt gtgtcggcta acacgtccgc
9241 gatcaaattt gtagttgagc tttttggaat tgcgatcgca taacttcgta tagcatacat
9301 tatacgaagt tataagctcg gaacgctgcg ctcggtcgtt cggctgcggc gagcggtatc
9361 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
9421 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
9481 tttccatagg ctccgccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
9541 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
9601 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
9661 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
9721 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
9781 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
9841 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
9901 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
9961 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
10021 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
10081 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
10141 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
10201 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
10261 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
10321 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
10381 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
10441 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
10501 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
10561 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
10621 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
10681 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
10741 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
10801 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
10861 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
10921 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
10981 ttattgtctc atgtccgcgc gtt
//
