pDisplay-GRAB_r5-HT1.0

LOCUS       pDisplay-GRAB_r5-HT1.0           8542 bp DNA     circular SYN 23-JAN-2024
DEFINITION  Expresses the red 5-HT sensor GRAB_r5-HT1.0 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pDisplay-GRAB_r5-HT1.0
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8542)
  TITLE     Dual-color GRAB sensors for monitoring spatiotemporal serotonin 
            release in vivo
REFERENCE   2  (bases 1 to 8542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 10, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8542
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        41..232
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(64..84)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     311..330
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             429..1223
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(483..502)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1093..1112
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1411..1430)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1455..1502
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1831..2419
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2320..2339
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    2551..2775
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2764..2781
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(2803..2949)
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             complement(2953..2982)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(3040..3756)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(3040..3756)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3082..3103)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3431..3450
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3690..3711
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    3768..4319
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3930..3949)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     4158..4175
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(4346..6337)
                     /codon_start=1
                     /label=r5-HT1.0
                     /translation="METDTLLLWVLLLWVPGSTGDTSLYKKVGTTGDKLDANVSSNEGF
                     RSVEKVVLLTFLAVVILMAILGNLLVMVAVCRDRQLRKIKTNYFIVSLAFADLLVSVLV
                     MPFGAIELVQDIWAYGEMFCLVRTSLDVYLCTASIFHLCCISLDRYYAICCQPLVYRNK
                     MTPLRIALMLGGCWVLPMFISFLPIMQGWNNIGIVDVIEKRKFSHNSNSTWCVFMVNKP
                     YAITCSVVAFYIPFLLMVLAYYRIYVTAKEHAQLWPVCSEVMYPEDGALKSEIVKGLRL
                     KDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQCERAEARHSTGGM
                     DELYKGGTGGSLVSKGEEHNHAIIKEFMRFKVHMEGSFNGHEFEIEGEGEGRPYEAFQT
                     AKLKVTKGGPLPFAWDILSPQFMYGSKAYIKHPADIPDYFKLSFPEGFRWERVMNFEDG
                     GRIHVLQDSSLQDGYFIYKVKLRGTNFPPDGPVMQKKTMGWELTVHDQHSTSRMRTETK
                     AAKTLCIIMGCFCFCWAPFFVTNIVDPFIDYTVPEQVWIAFLWLGYINSGLNPFLYAFL
                     NKSFRRAFLIILRCNYKVEKKPPVRQIPRVAATALSGRELNVNIYRHTNEPVIEKASDN
                     EPGIEMQVENLELPVNPSSVVSERISSV"
     primer_bind     complement(4852..4871)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(6250..6274)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     sig_peptide     complement(6275..6337)
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTGD"
     primer_bind     complement(6417..6436)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6418..6436)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(6481..6684)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6510..6530)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6685..7064
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(7076..7531)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7213..7234)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7425..7444
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7558..7662
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7663..8523
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7881..7900)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 catatatact ttagattgat ttattgaaaa aggaagagtc ctgaggcgga aagaaccagc
       61 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
      121 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
      181 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
      241 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
      301 taattttttt tatttatgca gaggccgagg cccggcctct gagctattcc agaagtagtg
      361 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
      421 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
      481 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
      541 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
      601 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
      661 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
      721 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
      781 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
      841 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
      901 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
      961 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     1021 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     1081 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     1141 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     1201 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     1261 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     1321 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     1381 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
     1441 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
     1501 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     1561 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     1621 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
     1681 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     1741 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1801 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1861 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1921 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1981 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2041 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgc gtcttaccgg
     2101 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2161 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2221 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2281 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2341 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2401 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2461 ctggcctttt gctcacatgt tctttcctgc gttatccccc gattctgtgg ataaccgtat
     2521 taccgccact ggttctttcc gcctcagaag ccatagagcc caccgcatcc ccagcatgcc
     2581 tgctattgtc ttcccaatcc tcccccttgc tgtcctgccc caccccaccc cccagaatag
     2641 aatgacacct actcagacaa tgcgatgcaa tttcctcatt ttattaggaa aggacagtgg
     2701 gagtggcacc ttccagggtc aaggaaggca cgggggaggg gcaaacaaca gatggctggc
     2761 aactagaagg cacagtcgag gctgatctcg agcggccgcc taacgtggct tcttctgcca
     2821 aagcatgatg aggatgataa gggagatgat ggtgagcacc accagggcca ggatggctga
     2881 gatcaccacc accttaaagg gcaaggagtg tggcaccacg atgacctcct gcgtgtcctg
     2941 gcccacagca ttcagatcct cttctgagat gagtttttgt tcgtcgactt acataattac
     3001 acactttgtc tttgacttct ttttcttctt tttaccatcc ttgtacagct cgtccatgcc
     3061 gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt
     3121 ggggtctttg ctcagggcgg actgggtgct caggtagtgg ttgtcgggca gcagcacggg
     3181 gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc tgccgtcctc
     3241 gatgttgtgg cggatcttga agttcacctt gatgccgttc ttctgcttgt cggccatgat
     3301 atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc
     3361 cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac
     3421 ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta
     3481 gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct
     3541 gaagcactgc acgccgtagg tcagggtggt cacgagggtg ggccagggca cgggcagctt
     3601 gccggtggtg cagatgaact tcagggtcag cttgccgtag gtggcatcgc cctcgccctc
     3661 gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac
     3721 caccccggtg aacagctcct cgcccttgct caccatggtt gtggccatat tatcatcgtg
     3781 tttttcaaag gaaaaccacg tccccgtggt tcggggggcc tagacgtttt tttaacctcg
     3841 actaaacaca tgtaaagcat gtgtaccgag gccccagatc agatcccata caatggggta
     3901 ccttctgggc atccttcagc cccttgttga atacgcttga ggagagccat ttgactcttt
     3961 ccacaactat ccaactcaca acgtggcact ggggttgtgc cgcctttgca ggtgtatctt
     4021 atacacgtgg ctttggccgc agaggcacct gtcgccaggt ggggggttcc gctgcctgca
     4081 aagggtcgct acagacgttg tttgtcttca agaagcttcc agaggaactg cttccttcac
     4141 gacatttaac agaccttgca ttcctttggc gagaggggaa agacccctag gaatgctcgt
     4201 caagaagaca gggccaggtt tccgggccct cacattgcca aaagacggca atatggtgga
     4261 aaataacata tagacaaacg cacaccggcc ttattccaag cggcttcggc cagtaacgtt
     4321 aggggggagg gagaggggcg ctagcttaca cactgctaat cctttcgcta accacactgg
     4381 agggatttac tggtaactct aaattctcaa cttgcatctc tataccgggc tcattgtcac
     4441 tggctttctc gatcaccggt tcattggtat gccgataaat gttaacatta agctccctcc
     4501 cagacaaagc agtggcggca actcttggaa tctgcctgac aggaggcttt ttctctacct
     4561 tataattgca acggaggatg atgaggaagg cacgtctgaa agacttattc aagaaggcat
     4621 agagaaaagg gttcaacccc gaattgatat agccaagcca gaggaaagcg atccacacct
     4681 gctcggggac agtgtagtct atgaaagggt ccacaatatt ggtgacaaag aagggggccc
     4741 agcagaaaca gaagcagccc atgatgatac ataaagtctt ggctgccttg gtctctgtcc
     4801 tcatgcgact tgtgctgtgc tggtcgtgca ccgtcagctc ccagcccatg gtcttcttct
     4861 gcattacggg gccgtcgggg gggaagttgg tgccgcgcag cttcaccttg tagatgaagt
     4921 agccgtcctg cagggaggag tcctgcagaa cgtgaatgcg gccgccgtcc tcgaagttca
     4981 tcacgcgctc ccacctgaag ccctcgggga aggacagctt gaagtagtcg gggatgtcgg
     5041 ctgggtgctt aatgtaggcc ttggagccgt acatgaactg aggggacagg atgtcccagg
     5101 cgaagggcag ggggccaccc ttggtcacct tcagcttagc ggtctgaaag gcctcgtagg
     5161 ggcggccctc gccctcgccc tcgatctcga actcgtggcc gttgaaggag ccctccatgt
     5221 gcaccttgaa gcgcatgaac tccttgatga tggcgtggtt gtgctcctcg cccttgctca
     5281 ccagactccc gcctgtacct cccttgtaca gctcgtccat gccgccggtg gagtggcggg
     5341 cctcggcgcg ttcgcactgt tccacgatgg tgtagtcctc gttgtgggac acgatgtcca
     5401 acttgatgtc gacgatgtag gcgccgggca gctgcacggg cttcttggcc ttgtaggtgg
     5461 tcttgacctc ggcggcgtag tggccgccgt ccttcagcct cagccccttc acgatctcgc
     5521 tcttcagggc gccgtcctcg gggtacataa cctcggaaca cacgggccag agctgggcat
     5581 gctccttagc agtgacatag attcggtaat aggccagcac catgaggaga aacgggatgt
     5641 agaaggccac cacagagcag gtgatagcat agggcttgtt gaccatgaag acacaccacg
     5701 tggagttaga gttgtggctg aatttccttt tctctatcac atcaactatg ccgatgttgt
     5761 tccagccttg cattatgggg agaaaagata taaacatggg aaggacccag cagcctccca
     5821 acattaatgc gatgcgtaga ggggtcatct tgttcctata aaccaaaggc tggcagcaga
     5881 tggcgtaata cctgtccagg gaaatacagc acaggtgaaa gatcgatgct gtacaaaggt
     5941 agacatccag agaggtccgg accaggcaga acatctcccc ataagcccag atgtcttgga
     6001 ccagctcaat ggcaccaaag ggcatcacca gcaccgaaac cagcaggtca gcaaaggcga
     6061 gagacacaat gaaatagttg gtttttattt tcctgagctg cctgtccctg cacacagcca
     6121 ccatcaccag caggttgccc aagatggcca tcaggataac cactgcaagg aacgtgagca
     6181 gcacgacctt ctccacggac ctgaaaccct cgttggaact cacattagca tcaagtttgt
     6241 caccggtggt gccaactttt ttgtacaaac ttgtgtcacc agtggaacct ggaacccaga
     6301 gcagcagtac ccatagcagg agtgtgtctg tctccatggt ggatatcccc aagccgaatt
     6361 ccagcacact ggcggccgtt actagtggat ccgagctcgg taccaagctt gggtctccct
     6421 atagtgagtc gtattaattt cgataagcca gtaagcagtg ggttctctag ttagccagag
     6481 agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc
     6541 ggagttgtta cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt
     6601 gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt
     6661 gatgtactgc caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg
     6721 ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc
     6781 gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg
     6841 ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac
     6901 tatgggaaca tacgtcatta ttgacgtcaa tgggcagggg tcgttgggcg gtcagccagg
     6961 cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga
     7021 ccccgtaatt gattactatt aataactagt caataatcaa tgtcaacgcg cgcgtaaatt
     7081 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     7141 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     7201 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     7261 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     7321 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     7381 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     7441 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     7501 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
     7561 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     7621 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     7681 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     7741 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     7801 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     7861 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     7921 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     7981 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     8041 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     8101 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     8161 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     8221 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     8281 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     8341 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     8401 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     8461 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     8521 taactgtcag accaagttta ct
//