pDisplay-GRAB_g5-HT1.0

LOCUS      pDisplay-GRAB_g5-HT1.0             8603 bp DNA     circular SYN 20-DEC-2024
DEFINITION  TSN20241124-0571-00027_777_20241125-BAN3_C03_C03_i10.1  (8603 bp).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 24, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    463..1048
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             complement(1056..3026)
                     /codon_start=1
                     /label=GRAB_HT1.0
                     /translation="TTGVNLRNAVHSFLVHLIGLLVWQCDISVSPVAAIVTDIFNTSDG
                     GRFKFPDGVQNWPALSIVIIIIMTIGGNILVIMAVSMEKKLHNATNYFLMSLAIADMLV
                     GLLVMPLSLLAILYDYVWPLPRYLCPVWISLDVLFSTASIMHLCAISLDRYVAIRNPIE
                     HSRFNSRTKAIMKIAIVWAISIGVSVPIPVIGLRDEEKVFVNNTTCVLNDPNFVLIGSF
                     VAFFIPLTIMVITYCLTIYVLRRQALMFLNGNVYIKADKQKNGIKANFHIRHNIEDGGV
                     QLAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDE
                     LYKGGTGGQESMSSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATTGKLTLKFI
                     CTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNGFATANNERKASKVLGIVFFV
                     FLIMWCPFFITNILSVLCEKSCNQKLMEKLLNVFVWIGYVCSGINPLVYTLFNKIYRRA
                     FSNYLRCNYKVEKKPPVRQIPRVAATALSGRELNVNIYRHTNEPVIEKASDNEPGIEMQ
                     VENLELPVNPSSVVSERISSV"
     protein_bind    complement(3026..3050)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     sig_peptide     complement(3051..3113)
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTGD"
     promoter        complement(3194..3212)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3257..3460)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        3461..3840
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3852..4307)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4334..4438
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4439..5299
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             5747..6541
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTYGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6773..6820
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7149..7737
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    7869..8093
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(8121..8267)
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             complement(8271..8300)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(join(8358..8603,1..462))
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 ccgctcggag gaggcctccc agcccatggt cttcttctgc attacggggc cgtcggaggg
       61 gaagttggtg ccgcgcagct tcaccttgta gatgaactcg ccgtcctgca gggaggagtc
      121 ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc acgcgctccc acttgaagcc
      181 ctcggggaag gacagcttca agtagtcggg gatgtcggcg gggtgcttca cgtaggcctt
      241 ggagccgtac atgaactgag gggacaggat gtcccaggcg aagggcaggg ggccaccctt
      301 ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggg cggccctcgc cctcgccctc
      361 gatctcgaac tcgtggccgt tcacggagcc ctccatgtgc accttgaagc gcatgaactc
      421 cttgatgatg gccatgttat cctcctcgcc cttgctcacc atggttgtgg ccatattatc
      481 atcgtgtttt tcaaaggaaa accacgtccc cgtggttcgg ggggcctaga cgttttttta
      541 acctcgacta aacacatgta aagcatgtgt accgaggccc cagatcagat cccatacaat
      601 ggggtacctt ctgggcatcc ttcagcccct tgttgaatac gcttgaggag agccatttga
      661 ctctttccac aactatccaa ctcacaacgt ggcactgggg ttgtgccgcc tttgcaggtg
      721 tatcttatac acgtggcgtt ggccgcagag gcacctgtcg ccaggtgggg ggttccgctg
      781 cctgcaaagg gtcgctacag acgttgtttg tcttcaagaa gcttccagag gaactgcttc
      841 cttcacgaca ttcaacagac cttgcattcc tttggcgaga ggggaaagac ccctaggaat
      901 gctcgtcaag aagacagggc caggtttccg ggccctcaca ttgccaaaag acggcaatat
      961 ggtggaaaat aacatataga caaacgcaca ccggccttat tccaagcggc ttcggccagt
     1021 aacgttaggg gggggggagg gagaggggcg ctagcttaca cactgctaat cctttcgcta
     1081 accacactgg agggatttac tggtaactct aaattctcaa cttgcatctc tataccgggc
     1141 tcattgtcac tggctttctc gatcaccggt tcattggtat gccgataaat gttaacatta
     1201 agctccctcc cagacaaagc agtggcggca actcttggaa tctgcctgac aggaggcttt
     1261 ttctctacct tataattgca acgcaaatag ttggagaatg cccttcggta aattttgttg
     1321 aacagagtat acaccagagg attgattcct gaacaaacat agccaatcca aacaaacaca
     1381 ttcagaagct tttccatgag cttttggtta caggacttct cacaaagaac agacagaata
     1441 ttggtaatga aaaatgggca ccacatgatc agaaacacaa agaaaacaat cccaaggact
     1501 ttcgaagctt ttctttcatt gtttgctgtt gcaaatccgt tgtactccag cttgtgcccc
     1561 aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg
     1621 tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg
     1681 gtgcgctcct ggatgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg
     1741 tggtcggggt agcggctgaa gcactgcacg ccgtaggtca gggtggtcac gagggtgggc
     1801 cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccagtggtg
     1861 gcatcgccct caccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc
     1921 tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctgct catggattct
     1981 tgccctccgg taccgccctt gtacagctcg tccatgccga gagtgattcc ggcggcggtc
     2041 acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttcga aagtttggac
     2101 tgcacgctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc
     2161 tgctggtagt ggtaggcgag ctgcacgccg ccgtcctcga tgttgtggcg gatgtggaag
     2221 ttcgccttga tgccgttctt ctgcttgtcg gccttgatat agacgtttcc gttcagaaac
     2281 atcaaagctt gtcggcgcag aacgtagatg gtcaggcaat acgtaatcac cataatcgtc
     2341 agcggtatga agaaagctac gaaggaccca ataagaacga aatttgggtc gttgagcacg
     2401 cacgtcgtgt tgttcacgaa caccttttct tcgtccctca gtccaatcac agggatagga
     2461 actgatacac ctatagaaat tgcccaaaca atagcaatct tcatgatggc cttagtccgc
     2521 gaattgaaac ggctatgctc aataggatta cgtattgcta cataccgatc cagcgatata
     2581 gcgcagaggt gcatgatgga cgctgttgaa aataaaacat ctaaagaaat ccagacgggg
     2641 cacaaatatc taggtagtgg ccagacataa tcataaagga ttgccaggag agacaggggc
     2701 atgacaagta gtcccactag catatcagca atggctaggg acattaagaa gtaattggtg
     2761 gcattgtgca gtttcttttc catgcttact gccatgatca caaggatgtt gccacctatt
     2821 gtcatgatta ttatgatgac gattgaaagt gctggccagt tttgtacccc gtctgggaat
     2881 ttgaagcgtc caccatcgga ggtattgaaa atgtcagtta ctatagctgc tactgggctc
     2941 acagaaatat cacattgcca aaccaatagg ccaattaggt gcacaaggaa tgaatgcacc
     3001 gcattcctca ggttcacacc ggtggtgcca acttttttgt acaaacttgt gtcaccagtg
     3061 gaacctggaa cccagagcag cagtacccat agcaggagtg tgtctgtctc catggtggat
     3121 atccccaagc cgaattccag cacactggcg gccgttacta gtggatccga gctcggtacc
     3181 aagcttgggt ctccctatag tgagtcgtat taatttcgat aagccagtaa gcagtgggtt
     3241 ctctagttag ccagagagct ctgcttatat agacctccca ccgtacacgc ctaccgccca
     3301 tttgcgtcaa tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc
     3361 aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc
     3421 gctatccacg cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta
     3481 atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc
     3541 aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt
     3601 gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag
     3661 tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg caggggtcgt
     3721 tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg
     3781 ggctatgaac taatgacccc gtaattgatt actattaata actagtcaat aatcaatgtc
     3841 aacgcgcgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     3901 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     3961 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     4021 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     4081 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     4141 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     4201 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     4261 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt
     4321 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     4381 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     4441 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     4501 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     4561 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     4621 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     4681 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     4741 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     4801 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     4861 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     4921 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     4981 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     5041 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     5101 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     5161 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     5221 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     5281 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     5341 attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg
     5401 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5461 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     5521 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccattc cgcccctaac
     5581 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     5641 ggccgaggcc cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     5701 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     5761 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     5821 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     5881 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     5941 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     6001 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     6061 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     6121 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     6181 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     6241 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
     6301 cgtgacctat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     6361 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     6421 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     6481 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     6541 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     6601 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     6661 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg
     6721 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
     6781 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
     6841 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
     6901 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
     6961 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta
     7021 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     7081 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     7141 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     7201 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     7261 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     7321 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     7381 gtggctgctg ccagtggcga taagtcgcgt cttaccgggt tggactcaag acgatagtta
     7441 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     7501 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     7561 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     7621 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     7681 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     7741 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     7801 tttcctgcgt tatcccccga ttctgtggat aaccgtatta ccgccactgg ttctttccgc
     7861 ctcagaagcc atagagccca ccgcatcccc agcatgcctg ctattgtctt cccaatcctc
     7921 ccccttgctg tcctgcccca ccccaccccc cagaatagaa tgacacctac tcagacaatg
     7981 cgatgcaatt tcctcatttt attaggaaag gacagtggga gtggcacctt ccagggtcaa
     8041 ggaaggcacg ggggaggggc aaacaacaga tggctggcaa ctagaaggca cagtcgaggc
     8101 tgatctcgag cggccgccta acgtggcttc ttctgccaaa gcatgatgag gatgataagg
     8161 gagatgatgg tgagcaccac cagggccagg atggctgaga tcaccaccac cttaaagggc
     8221 aaggagtgtg gcaccacgat gacctcctgc gtgtcctggc ccacagcatt cagatcctct
     8281 tctgagatga gtttttgttc gtcgacttac ataattacac actttgtctt tgacttcttt
     8341 ttcttctttt taccatcctt gtacagctcg tccatgccgc cggtggagtg gcggccctcg
     8401 gcgcgttcgt actgttccac gatggtgtag tcctcgttgt gggaggtgat gtccaacttg
     8461 atgttgacgt tgtaggcgcc gggcagctgc acgggcttct tggccttgta ggtggtcttg
     8521 acctcagcgt cgtagtggcc gccgtccttc agcttcagcc tctgcttgat ctcgcccttc
     8581 agggcgccgt cctcggggta cat
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