| 质粒名称: | pYK97 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000791 |
| 产品用途: | Mammalian PhCMV-driven constitutive NS3a(H1)-AG-FTH1 expression vector (PhCMV-NS3a(H1)-AG-FTH1-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | NS3a(H1)-AG-FTH1 |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK97
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK97载体质粒基本信息
pYK97质粒图谱载体图谱和pYK97载体序列质粒序列多克隆位点信息
pYK97质粒载体简介
pYK97质粒序列载体序列
LOCUS pYK97 7542 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7542)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 24, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7542
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 70..147
/label=SV40 ORI
CDS 289..1080
/label=Kan/neoR
misc_feature 1254..1384
/label=SV40 early polyadenylation signal
primer_bind complement(1420..1440)
/label=M13-rev
misc_binding complement(1446..1468)
/label=LacO
promoter complement(1473..1502)
/label=lac
misc_feature complement(1767..2440)
/label=pUC origin
CDS complement(2588..3247)
/label=AmpR
primer_bind 2904..2915
/label=oyk03-03-F
primer_bind 2904..2915
/label=oYK04-ns3a-F
promoter complement(3487..3515)
/label=Amp prom
misc_feature 3813..4400
/label=CMV
primer_bind 4444..4463
/label=T7
primer_bind 4462..4509
/label=oYK317_NLS_NS3a_F
primer_bind 4462..4508
/label=oYK30-NS3a_Head-F
primer_bind 4462..4490
/label=oYK36-AG-Link_s-F
primer_bind 4462..4481
/label=oYK06-ANR-mEGFP-F
CDS 4491..5078
/codon_start=1
/label=NS3a H1
/translation="KKKGSVVIVGRINLSGDTAYSQQTRGLEGCQETSQTGRDKNQVEG
EVQVVSTATQSFLATSINGVLWTVYHGAGTRTIASPKGPVTQMYTNVDKDLVGWQAPQG
SRSLTPCTCGSSDLYLVTRHADVIPVRGRGDSRGSLLSPRPISYLKGSSGGPLLCPAGH
AVGIFRAAVSTRGVAKAVDFIPVESLETTMRSP"
primer_bind 4491..4526
/label=oSCL1082
primer_bind 4491..4511
/label=oyk03-03-F
primer_bind 4491..4511
/label=oYK04-ns3a-F
primer_bind 4491..4509
/label=oYK25-NS3a-F
primer_bind 4508..4566
/label=oSCL1083
primer_bind 4547..4605
/label=oSCL1084
primer_bind 4584..4642
/label=oSCL1085
primer_bind 4621..4679
/label=oSCL1086
primer_bind complement(4649..4668)
/label=oSCL1106
primer_bind 4650..4688
/label=oSCL1104
primer_bind complement(4885..4943)
/label=oSCL1105
primer_bind complement(4925..4996)
/label=oSCL1087
primer_bind 4927..4948
/label=oSCL1107
primer_bind 4978..4996
/label=oSCL1088
primer_bind complement(5060..5084)
/label=oSCL1089
primer_bind complement(5063..5095)
/label=oYK30-NS3a_head-R
primer_bind complement(5066..5107)
/label=oYK66-NS3a-R
misc_feature 5085..5095
/label=linker
CDS 5088..5162
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind 5088..5108
/label=oYK66-AG-lplA-F
primer_bind 5088..5106
/label=Primer 1
primer_bind complement(5137..5165)
/label=olyl212
primer_bind complement(5137..5162)
/label=olyl234
primer_bind complement(5146..5182)
/label=oYK45-46 ANR1-R
primer_bind 5162..5181
/label=oYK36-AG-Link_s-F
primer_bind 5163..5182
/label=oYK41-44 AG-LplA-F
primer_bind 5163..5182
/label=oYK45-46 AG-lplA-F
misc_feature 5163..5165
/label=Long_Linker
misc_feature 5166..5840
/label=AG
primer_bind 5614..5624
/label=oSCL1104
primer_bind 5821..5857
/label=oYK37-Long_Link-F
primer_bind complement(5825..5840)
/label=oYK37-AG-R
misc_feature 5841..6068
/label=Long_Linker
primer_bind complement(6048..6068)
/label=oYK81_ANR_AG_R
primer_bind 6049..6085
/label=oYK81_FTH_F
primer_bind complement(6049..6071)
/label=oYK37-Long_Link-R
/note="AGGCGTAATGTGGAgcCCATCATGGATCCTCCTTGGGTCGATC"
gene 6068..6621
/gene="FTH1"
/gene_synonym="FHC; FTH; FTHL6; HFE5; PIG15; PLIF"
/label=FTH1
/note="ferritin heavy chain 1"
/db_xref="GeneID:2495"
/db_xref="HGNC:HGNC:3976"
/db_xref="MIM:134770"
primer_bind 6068..6085
/label=oCW57
primer_bind 6068..6082
/label=oCW29
CDS 6069..6620
/codon_start=1
/gene="FTH1"
/gene_synonym="FHC; FTH; FTHL6; HFE5; PIG15; PLIF"
/product="ferritin heavy chain"
/EC_number="1.16.3.1"
/label=FTH1
/note="apoferritin; placenta immunoregulatory
factor;ferritin, heavy polypeptide 1;
proliferation-inducing protein 15; ferritin H subunit; cell
proliferation-inducing gene 15 protein"
/db_xref="CCDS:CCDS41655.1"
/db_xref="GeneID:2495"
/db_xref="HGNC:HGNC:3976"
/db_xref="MIM:134770"
/protein_id="NP_002023.2"
/translation="MTTASTSQVRQNYHQDSEAAINRQINLELYASYVYLSMSYYFDRD
DVALKNFAKYFLHQSHEEREHAEKLMKLQNQRGGRIFLQDIKKPDCDDWESGLNAMECA
LHLEKNVNQSLLELHKLATDKNDPHLCDFIETHYLNEQVKAIKELGDHVTNLRKMGAPE
SGLAEYLFDKHTLGDSDNES"
misc_feature 6069..6071
/gene="FTH1"
/gene_synonym="FHC; FTH; FTHL6; HFE5; PIG15; PLIF"
/note="N-acetylmethionine.
/evidence=ECO:0007744|PubMed:22814378; propagated from
UniProtKB/Swiss-Prot (P02794.2); acetylation site"
misc_feature 6072..6074
/gene="FTH1"
/gene_synonym="FHC; FTH; FTHL6; HFE5; PIG15; PLIF"
/note="N-acetylthreonine, in Ferritin heavy
chain,N-terminally processed.
/evidence=ECO:0007744|PubMed:22814378; propagated from
UniProtKB/Swiss-Prot (P02794.2); acetylation site"
primer_bind complement(6593..6640)
/label=oYK81_FTH1_R
primer_bind complement(6593..6617)
/label=oYK236_FTH1
primer_bind complement(6597..6664)
/label=oCW30
misc_feature 6603..6605
/gene="FTH1"
/gene_synonym="FHC; FTH; FTHL6; HFE5; PIG15; PLIF"
/note="Phosphoserine./evidence=ECO:0007744|PubMed:17081983,
ECO:0007744|PubMed:18318008, ECO:0007744|PubMed:20068231,
ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:24275569;
propagated from UniProtKB/Swiss-Prot (P02794.2);
phosphorylation site"
misc_feature 6615..6617
/gene="FTH1"
/gene_synonym="FHC; FTH; FTHL6; HFE5; PIG15; PLIF"
/note="Phosphoserine./evidence=ECO:0007744|PubMed:18318008;
propagated from UniProtKB/Swiss-Prot (P02794.2);
phosphorylation site"
primer_bind complement(6621..6643)
/label=oYK57-MCP-mCherry-R
primer_bind complement(6645..6664)
/label=oSCL1089
terminator 6717..6944
/label=bGH PA
misc_feature 6990..7418
/label=f1 origin
misc_feature join(7423..7542,1..224)
/label=SV40 early promoter and origin
ORIGIN
1 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
61 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
121 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
181 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
241 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
301 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
361 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
421 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
481 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
541 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
601 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
661 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
721 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
781 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
841 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
901 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
961 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
1021 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
1081 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
1141 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
1201 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
1261 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
1321 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
1381 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
1441 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
1501 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
1561 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
1621 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
1681 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
1741 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
1801 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
1861 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
1921 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
1981 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
2041 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
2101 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
2161 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
2221 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
2281 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
2341 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
2401 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
2461 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
2521 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
2581 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
2641 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
2701 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
2761 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
2821 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
2881 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
2941 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
3001 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
3061 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
3121 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
3181 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
3241 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
3301 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
3361 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
3421 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
3481 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
3541 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
3601 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
3661 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
3721 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
3781 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
3841 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
3901 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
3961 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
4021 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
4081 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
4141 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
4201 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
4261 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
4321 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
4381 ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga
4441 aattaatacg actcactata gggagaccca agctggctag cgccaccatg aagaagaagg
4501 gcagcgtggt gatcgtgggc agaatcaacc tgagcggcga caccgcctac agccagcaga
4561 ccagaggcct ggagggctgc caggagacca gccagaccgg cagagacaag aaccaggtgg
4621 agggcgaggt gcaggtggtg agcaccgcca cccagagctt cctggccacc agcatcaacg
4681 gcgtgctgtg gaccgtgtac cacggcgccg gcaccagaac catcgccagc cccaagggcc
4741 ccgtgaccca gatgtacacg aacgtggaca aggacttagt gggctggcag gccccccaag
4801 gcagcagaag cctgaccccc tgcacctgcg gcagcagcga cctgtacctg gtgaccagac
4861 acgccgacgt gatccccgtg agaggaagag gcgacagcag aggcagcctg ctgagcccca
4921 gacccatcag ctatctgaaa ggctcaagcg gcggccccct gttgtgtccc gccggccatg
4981 ccgtgggcat cttcagagcc gccgtgagca ccagaggcgt ggccaaggcc gtggacttca
5041 tccccgtgga gagcctggag accaccatga gaagccccgc tagcagcgga agtggaactg
5101 ggtctgggac tgggtccggc accgggacta cttcaggcac cggcactggg ggctccactg
5161 gcatggtgag cgtgatcaag cccgagatga agatcaagct gtgcatgagg ggcaccgtga
5221 acggccacaa cttcgtgatc gagggcgagg gcaagggcaa cccctacgag ggcacccaga
5281 tcctggacct gaacgtgacc gagggcgccc ccctgccctt cgcctacgac atcctgacca
5341 ccgtgttcca gtacggcaac agggccttca ccaagtaccc cgccgacatc caggactact
5401 tcaagcagac cttccccgag ggctaccact gggagaggag catgacctac gaggaccagg
5461 gcatctgcac cgccaccagc aacatcagca tgaggggcga ctgcttcttc tacgacatca
5521 ggttcgacgg cgtgaacttc ccccccaacg gccccgtgat gcagaagaag accctgaagt
5581 gggagcccag caccgagaag atgtacgtga gggacggcgt gctgaagggc gacgtgaaca
5641 tggccctgct gctggagggc ggcggccact acaggtgcga cttcaagacc acctacaagg
5701 ccaagaagga cgtgaggctg cccgactacc acttcgtgga ccacaggatc gagatcctga
5761 agcacgacaa ggactacaac aaggtgaagc tgtacgagaa cgccgtggcc aggtacagca
5821 tgctgcccag ccaggccaag accggttctg ggagtggcgg tagtggtgct ggaggcagcg
5881 caggctctgg agcaggcggc agtgccggaa gtggcgctgg agggtctgcc ggatctggag
5941 ccggtggttc tgccggctcc ggcgctggtg ggagcgcttc cggaagtagt cccggtaatt
6001 ccgctgacgg cggcggagga tcgggtggta gtggtggttc aggaggagga tcgacccaag
6061 gaggatccat gacgaccgcg tccacctcgc aggtgcgcca gaactaccac caggactcag
6121 aggccgccat caaccgccag atcaacctgg agctctacgc ctcctacgtt tacctgtcca
6181 tgtcttacta ctttgaccgc gatgatgtgg ctttgaagaa ctttgccaaa tactttcttc
6241 accaatctca tgaggagagg gaacatgctg agaaactgat gaagctgcag aaccaacgag
6301 gtggccgaat cttccttcag gatatcaaga aaccagactg tgatgactgg gagagcgggc
6361 tgaatgcaat ggagtgtgca ttacatttgg aaaaaaatgt gaatcagtca ctactggaac
6421 tgcacaaact ggccactgac aaaaatgacc cccatttgtg tgacttcatt gagacacatt
6481 acctgaatga gcaggtgaaa gccatcaaag aattgggtga ccacgtgacc aacttgcgca
6541 agatgggagc gcccgaatct ggcttggcgg aatatctctt tgacaagcac accctgggag
6601 acagtgataa tgaaagctaa ggatccacta gtccagtgtg gtggaattct gcagatatcc
6661 agcacagtgg cggccgctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac
6721 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
6781 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
6841 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
6901 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
6961 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
7021 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
7081 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
7141 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
7201 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
7261 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
7321 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
7381 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
7441 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
7501 aattagtcag caaccaggtg tggaaagtcc ccaggctccc ca
//
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