| 质粒名称: | pSL1545 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002450 |
| 产品用途: | AAV2-vector for PhCMV-driven constitutive nVEGFi expression (ITR(AAV)-PhCMV-nVEGFi-pA-ITR(AAV)). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pX601 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | PhCMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | nVEGFi |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Rationally designed light-inducible RNA-releasing protein for translational regulation and optogenetic control of gene therapies
NA
NA
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pSL1545
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSL1545载体质粒基本信息
pSL1545质粒图谱载体图谱和pSL1545载体序列质粒序列多克隆位点信息
pSL1545质粒载体简介
pSL1545质粒序列载体序列
LOCUS pSL1545 5526 bp DNA circular SYN 11-FEB-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5526)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Aug 30, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5526
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR (alternate)
/note="Functional equivalent of wild-type AAV2 ITR"
primer_bind 115..136
/label=oSCL1014
enhancer 154..533
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 534..737
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 688..708
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 750..776
/label=oSCL1017
misc_feature 763
/label=NheI
primer_bind 806..854
/label=oSCL2527
misc_feature 832..2514
/label=nVEGFi
CDS 832..909
/codon_start=1
/label=Signal peptide
/translation="MVSYWDTGVLLCALLSCLLLTGSSSG"
CDS 910..1200
/codon_start=1
/label=VEGFR1 IgG1
/translation="SKLKDPELSLKGTQHIMQAGQTLHLQCRGEAAHKWSLPEMVSKES
ERLSITKSACGRNGKQFCSTLTLNTAQANHTGFYSCKYLAVPTSKKKETESA"
CDS 1201..1464
/codon_start=1
/label=VEGFR1 IgG2
/translation="IYIFISDTGRPFVEMYSEIPEIIHMTEGRELVIPCRVTSPNITVT
LKKFPLDTLIPDGKRIIWDSRKGFIISNATYKEIGLLTCEATV"
CDS 1465..1830
/codon_start=1
/label=VEGFR2 IgG3
/translation="NGHLYKTNYLTHRQTNTIIDVVLSPSHGIELSVGEKLVLNCTART
ELNVGIDFNWEYPSSKHQHKKLVNRDLKTQSGSEMKKFLSTLTIDGVTRSDQGLYTCAA
SSGLMTKKNSTFVRVHEK"
CDS 1831..2511
/codon_start=1
/label= IgG FC
/translation="DKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVD
VSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVS
NKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWES
NGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLS
LSPGK"
primer_bind complement(2495..2534)
/label=oSCL2528
primer_bind complement(2529..2547)
/label=oYW306
terminator 2555..2782
/label=bGH PA
primer_bind complement(2764..2804)
/label=oSCL1014
primer_bind complement(2764..2786)
/label=oSCL1018
repeat_region 2789..2929
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 2789..2918
/label=AAV2 ITR
rep_origin 3004..3459
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3091..3110)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3301..3322
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(3476..3495)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 3595..3617
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(3655..3673)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 3741..3845
/gene="bla"
/label=AmpR promoter
CDS 3846..4706
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4064..4083)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 4877..5465
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5366..5385
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggcctcta gactcgaggc gttgacattg attattgact agttattaat
181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
541 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
661 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
721 tctatataag cagagctctc tggctaacta ccggccaagc tggctagcgt ttaaacttaa
781 gcttggtacc gagctcggat ccactagtcc agtgtggtgg aattcgccac catggtttct
841 tactgggata ccggcgtgct gctgtgtgcc ctgctgtctt gtctgctgct gaccggctct
901 agcagcggca gcaagctgaa ggatcccgag ctgagcctga agggcaccca gcatattatg
961 caggccggcc agacactgca cctccagtgt agaggcgaag ccgctcacaa atggtccctg
1021 cctgagatgg tgtccaaaga gagcgagcgg ctgagcatca ccaagagcgc ctgtggcaga
1081 aacggcaagc agttctgcag caccctgaca ctgaatactg cccaggccaa ccacaccggc
1141 ttctacagct gcaagtatct ggccgtgcct accagcaaga agaaagaaac cgagagcgcc
1201 atctacatct tcatctctga taccggcaga cccttcgtgg aaatgtacag cgagatcccc
1261 gagatcatcc acatgaccga gggcagagag ctggtcatcc cctgcagagt gacaagcccc
1321 aacatcaccg tgactctgaa gaagttccct ctggacacac tgatccccga cggcaagaga
1381 atcatctggg acagccggaa gggcttcatc atcagcaacg ccacctacaa agagatcggc
1441 ctgctgacct gtgaagccac cgtgaatggc cacctgtaca agaccaacta cctgacacac
1501 agacagacca acaccatcat cgacgtggtg ctgagcccta gccacggcat tgaactgtct
1561 gtgggcgaga agctggtgct gaactgtacc gccagaaccg agctgaacgt gggcatcgac
1621 ttcaactggg agtaccccag cagcaagcac cagcacaaga aactggtcaa ccgggacctg
1681 aaaacccaga gcggcagcga gatgaagaaa ttcctgagca ccctgaccat cgacggcgtg
1741 accagatctg accagggcct gtatacttgt gccgccagct ctggcctgat gaccaagaaa
1801 aacagcacct tcgtgcgggt gcacgagaag gacaagaccc acacctgtcc tccatgtcct
1861 gctccagaac tgctcggcgg accttccgtg ttcctgtttc ctccaaagcc taaggacacc
1921 ctgatgatca gcagaacccc tgaagtgacc tgcgtggtgg tggatgtgtc ccacgaggat
1981 cccgaagtga agttcaattg gtacgtggac ggcgtggaag tgcacaacgc caagaccaag
2041 cctagagagg aacagtacaa tagcacctac agagtggtgt ccgtgctgac cgtgctgcac
2101 caggattggc tgaacggcaa agagtacaag tgcaaggtgt ccaacaaggc cctgcctgct
2161 cctatcgaga aaaccatctc caaggccaag ggccagccta gggaacccca ggtttacaca
2221 ctgcctccaa gcagggacga gctgacaaag aaccaggtgt ccctgacctg cctggtcaag
2281 ggcttctacc cttccgatat cgccgtggaa tgggagagca atggccagcc tgagaacaac
2341 tacaagacaa cccctcctgt gctggacagc gacggctcat tcttcctgta cagcaagctg
2401 acagtggaca agagcagatg gcagcaggga aacgtgttca gctgcagcgt gatgcacgag
2461 gccctgcaca accactacac ccagaagtcc ctgagcctgt ctcctggcaa ataactcgag
2521 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
2581 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
2641 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
2701 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
2761 ggggatgcgg tgggctctat ggggccgcag gaacccctag tgatggagtt ggccactccc
2821 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
2881 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
2941 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
3001 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
3061 ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg
3121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
3181 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
3241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
3301 gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt
3361 tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat
3421 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
3481 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
3541 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
3601 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
3661 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
3721 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
3781 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
3841 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
3901 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
3961 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
4021 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
4081 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
4141 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
4201 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
4261 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
4321 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
4381 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
4441 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
4501 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
4561 gaagccgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
4621 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
4681 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
4741 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
4801 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
4861 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
4921 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
4981 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
5041 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
5101 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
5161 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
5221 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
5281 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
5341 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
5401 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
5461 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
5521 acatgt
//
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