| 质粒名称: | pAAVLINK-EF1a-HA-5'-DOCK8 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001705 |
| 产品用途: | 5' AAVLINK plasmid for DOCK8 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | DOCK8 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-DOCK8
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-DOCK8载体质粒基本信息
pAAVLINK-EF1a-HA-5'-DOCK8质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-DOCK8载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-DOCK8质粒载体简介
pAAVLINK-EF1a-HA-5'-DOCK8质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-DOCK8 6728 bp DNA circular SYN 21-JAN-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6728)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 3, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6728
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..3868
/codon_start=1
/label=DOCK8
/translation="MATLPSAERRAFALKINRYSSAEIRKQFTLPPNLGQYHRQSISTS
GFPSLQLPQFYDPVEPVDFEGLLMTHLNSLDVQLAQELGDFTDDDLDVVFTPKECRTLQ
PSLPEEGVELDPHVRDCVQTYIREWLIVNRKNQGSPEICGFKKTGSRKDFHKTLPKQTF
ESETLECSEPAAQAGPRHLNVLCDVSGKGPVTACDFDLRSLQPDKRLENLLQQVSAEDF
EKQNEEARRTNRQAKLSALYPSVDEEDAVEIRPVPECPKEHLGNRILVKLLTLKFEIEI
EPLFASIALYDVKERKKISENFHCDLNSDQFKGFLRAHTPSVAASSQARSAVFSVTYPS
SDIYLVVKIEKVLQQGEIGDCAEPYTVIKESDGGKSKEKIEKLKLQAESFCQRLGKYRM
PFAWAPISLSSFFNVSTLEREVTDVDSVVGRSSVGERRTLAQSRRLSERALSLEENGVG
SNFKTSTLSVSSFFKQEGDRLSDEDLFKFLADYKRSSSLQRRVKSIPGLLRLEISTAPE
IINCCLTPEMLPVKPFPENRTRPHKEILEFPTREVYVPHTVYRNLLYVYPQRLNFVNKL
ASARNITIKIQFMCGEDASNAMPVIFGKSSGPEFLQEVYTAVTYHNKSPDFYEEVKIKL
PAKLTVNHHLLFTFYHISCQQKQGASVETLLGYSWLPILLNERLQTGSYCLPVALEKLP
PNYSMHSAEKVPLQNPPIKWAEGHKGVFNIEVQAVSSVHTQDNHLEKFFTLCHSLESQV
TFPIRVLDQKISEMALEHELKLSIICLNSSRLEPLVLFLHLVLDKLFQLSVQPMVIAGQ
TANFSQFAFESVVAIANSLHNSKDLSKDQHGRNCLLASYVHYVFRLPEVQRDVPKSGAP
TALLDPRSYHTYGRTSAAAVSSKLLQARVMSSSNPDLAGTHSAADEEVKNIMSSKIADR
NCSRMSYYCSGSSDAPSSPAAPRPASKKHFHEELALQMVVSTGMVRETVFKYAWFFFEL
LVKSMAQHVHNMDKRDSFRRTRFSDRFMDDITTIVNVVTSEIAALLVKPQK"
misc_feature 1384..1389
/label=SDS
misc_feature 1428..1433
/label=SDS
misc_feature 1488..1493
/label=SDS
misc_feature 2680..2685
/label=SDS
misc_feature 3367..3372
/label=SDS
intron 3869..3909
/label=Splicing Donor
misc_feature 3913..3946
/label=lox2272-JT1571
repeat_region 3991..4131
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3991..4120
/label=AAV2 ITR
rep_origin 4206..4661
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4943..5047
/gene="bla"
/label=AmpR promoter
CDS 5048..5908
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6079..6667
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat ggccactctg ccgagcgcag agcgccgcgc
781 gttcgcgctc aagatcaaca ggtattcttc agcggaaata aggaaacagt ttactctccc
841 accaaacctt ggccagtacc atcgacagag cataagtacc tctggcttcc cctctcttca
901 actacctcag ttttatgacc ctgtggagcc agtggacttt gaaggacttc tgatgacaca
961 cctgaacagc ctggatgtgc agcttgccca ggagctcggg gacttcactg atgacgactt
1021 ggacgtggtg ttcacgccaa aggaatgtag gactttgcag ccctctttgc cggaggaagg
1081 ggttgaactg gaccctcatg tcagggactg tgttcagacc tacatccgtg agtggctaat
1141 cgtgaaccgg aaaaaccaag gaagtccaga aatctgtggc tttaaaaaga ctggatctcg
1201 aaaagatttt cacaagacgc ttccgaaaca gacgtttgag tcggaaacct tggagtgcag
1261 tgaacccgct gctcaggcag gcccccgcca cttaaacgtg ctgtgcgacg tgtctgggaa
1321 aggccccgtc actgcctgtg actttgacct ccgcagcctg cagcctgaca agcggctaga
1381 aaacctcctg cagcaagtga gtgccgagga ctttgagaag cagaacgagg aggcccggag
1441 gaccaatagg caggccaagc tctctgccct ttacccatca gtggacgagg aggatgctgt
1501 ggaaatacgt ccagtaccag aatgtcccaa ggaacacctg ggcaacagaa tattggtcaa
1561 gttgctgacc ttgaagttcg agattgaaat tgagcccctg tttgccagca ttgccctcta
1621 cgatgttaaa gaaaggaaaa agatctcaga aaattttcac tgtgacctga actctgacca
1681 gttcaaagga tttctgcgag ctcacacgcc ttcagtggcc gcatcaagtc aggcgagatc
1741 tgcagtcttc tcagtcacct acccgtcctc agacatctac ctggtagtca agattgaaaa
1801 agtcctgcag cagggagaga ttggagactg tgcagagccc tacacggtta tcaaagaaag
1861 tgatggtgga aagagtaaag aaaagattga aaaactaaaa ctccaagctg aatccttctg
1921 ccagcgtttg gggaaatacc ggatgccctt tgcctgggca cccataagct tatcaagctt
1981 cttcaatgtc tccacccttg agagggaggt aactgatgtg gactctgtgg ttgggagaag
2041 ctcagtgggt gaacggagga cattggccca atctagaagg ctttctgaaa gagccctctc
2101 cttggaggaa aatggggttg gatccaactt caaaacctcc actctgagcg ttagcagctt
2161 tttcaagcag gaaggagatc gccttagcga tgaagactta ttcaagtttt tagctgacta
2221 caaaagatca tcatccttac agagacgagt caagtcaatt ccaggcttgc taagactgga
2281 gatttctaca gctccagaga tcatcaattg ctgtctgact cctgaaatgc tgcccgtgaa
2341 accctttcct gaaaaccgga cacgcccgca caaagagatt ttggaatttc caacacgaga
2401 agtatatgtc cctcacactg tgtacagaaa ccttctctat gtctacccac agaggctgaa
2461 ctttgtaaac aaactagcat cagcccggaa cattacaata aagatccagt ttatgtgtgg
2521 agaagatgct agcaatgcga tgccggtcat ctttggaaaa tccagcgggc ctgaatttct
2581 gcaggaagtg tacacagctg ttacatacca taataagtct cctgactttt atgaagaagt
2641 gaaaattaag ctccccgcta agctcacagt aaatcaccac ctcctgttca ccttctacca
2701 tatcagctgt cagcagaagc aaggagcctc cgtggaaact ctcctgggat attcatggct
2761 gccaattctc ttaaatgaac gtcttcaaac tggatcctac tgtctcccag ttgccttgga
2821 aaaattgcca cccaactact ccatgcattc tgctgagaaa gtcccattac agaatcctcc
2881 cattaagtgg gctgaaggac ataagggagt atttaatatt gaagtgcaag ctgtttcttc
2941 tgtacacacc caggacaacc acctggagaa gttcttcacc ctctgccact ccctggagag
3001 ccaggtgacc ttccccatcc gcgtgctgga tcagaaaatc agcgagatgg cgctggagca
3061 tgagctgaag ctcagcatca tctgcctgaa ctcctcccgc ctggagccgc tcgtgctctt
3121 cctgcacctg gtgctggaca agctcttcca gctgtccgtg cagcccatgg tcatcgctgg
3181 ccagacagcc aacttctccc agtttgcctt cgagtccgtg gtggccatcg ccaacagtct
3241 gcacaacagc aaggacctga gcaaggacca gcatgggagg aactgcctgc tggcttccta
3301 cgtgcactac gtcttccgcc tgccagaggt gcaaagggat gtgcccaagt caggcgctcc
3361 cactgccctc ctagaccctc ggagctacca cacgtatggc cgcacatcag ctgctgctgt
3421 gagttcaaag ctgctgcagg cccgggtgat gagcagcagt aacccagacc tcgcggggac
3481 acactccgca gcagacgagg aagtgaagaa catcatgtct tcaaagatcg ccgatcgcaa
3541 ctgcagccga atgtcttact attgctctgg cagtagtgat gctccaagtt cacctgcagc
3601 cccaaggcca gccagcaaaa agcatttcca tgaggagctt gcccttcaga tggtggtcag
3661 caccggaatg gtgagagaaa cagtcttcaa gtatgcctgg ttcttctttg agcttctggt
3721 gaaaagcatg gcccagcacg tacataacat ggacaaacgg gacagttttc ggaggactcg
3781 tttttctgac cgtttcatgg atgacataac tactattgtt aatgtggtca cctcggaaat
3841 tgcagccctt ttagtaaaac cacagaaggt aagtatcaag gttacaagac aggtttaagg
3901 agaccaataa gctattattc gtataggata ctttatacga agttattaag ttatggagac
3961 cggtgtggca cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc
4021 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
4081 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga
4141 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
4201 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
4261 gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct
4321 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
4381 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
4441 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
4501 tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga
4561 tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa
4621 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
4681 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
4741 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
4801 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
4861 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
4921 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
4981 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
5041 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
5101 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
5161 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
5221 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
5281 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
5341 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
5401 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
5461 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
5521 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
5581 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
5641 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
5701 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
5761 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
5821 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
5881 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
5941 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
6001 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
6061 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
6121 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
6181 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
6241 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
6301 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
6361 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
6421 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
6481 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
6541 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
6601 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
6661 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
6721 tcacatgt
//
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