| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-SCN8A-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001420 |
| 产品用途: | 3' AAVLINK plasmid for SCN8A expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | SCN8A |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-SCN8A-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-SCN8A-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-SCN8A-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-SCN8A-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-SCN8A-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-SCN8A-FLAG 7218 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7218)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 7, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
FEATURES Location/Qualifiers
source 1..7218
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3931
/codon_start=1
/label=SCN8A
/translation="VVSSGSSAARSTSRKG*ASLGGSCGKPASSSWSTTGLRPSSSS*F
C*AVAPWPSRTSTLSRERPSAPSWNMLTKSSPISSSWRCCSSGQPMASSSSSPMPGVGW
TSSLWLSL*SAL*LMPWATRN*VP*SPLGP*EL*DP*EPYHDLKG*GWW*MPWWAPSPP
S*MCCWCVSSSG*FSASWELTCLRESTTTALMRLLKSDLKLKMSTIKLNVKSLWRGTIQ
RSDGRT*RSTLTMLGQDTWPFFK*QPSKAGWTSCMQL*IPGSLMSSLSMRTISTCTSIL
SSSSSSAPSSP*TCSLVSSLITSINKRKSSEVRTSS*PKNRRSTTMP*KSWAQRSHRNL
FPAP*TKSKESSLILSLSKPLTLLS*CSSALTW*Q*WWRQTLKASRWRTSSTGLTWCLL
SSSPVSVCSKCLR*GTTTSPLAGTSSTSW*SSSPLWECSWQI*LRNTLFPQPYSESSDW
PVLGASCV*SKAPKGFVPCSLP**CPCLPCSTSAFCSSWSCSSSPFLGCPILHM*STRL
VSMTCSTLRHLATA*SACFKSQPQLVGMACCCPS*TAPLTAA*IRNTQGVALREIVGTP
QWASSSL*ATSSSLS*LS*TCTLPSSWRTSV*PQRKVQTL*VRMTLRPSMRSGRSSTPM
PPSSLSTVSWQTLQMPWSILSECPSPIPLSSSLWICQW*AGIASTAWTSFLPSPSGSWE
IAGSWTSCGSRWKSGSWHPILPKCLTSQSQPHCVASRRRYLQWSCSVPTGDIWQGGASS
AKRQLLISWRMEAHTGRKKRAPHLQPPSRPMTV*LNLKRRNSSGQRKEEGKEPKDKKRS
ENPS"
misc_feature 3672..3677
/label=SDS
misc_feature 3675..3680
/label=SDS
CDS 3938..4003
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 4007..4438
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4151..4156
/label=SDS
repeat_region 4481..4621
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4481..4610
/label=AAV2 ITR
rep_origin 4696..5151
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5433..5537
/gene="bla"
/label=AmpR promoter
CDS 5538..6398
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6569..7157
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggttgtgtcc agcggttcaa
1501 gtgctgccag gtcaacatcg aggaagggct aggcaagtct tggtggatcc tgcggaaaac
1561 ctgcttcctc atcgtggagc acaactggtt tgagaccttc atcatcttca tgattctgct
1621 gagcagtggc gccctggcct tcgaggacat ctacattgag cagagaaaga ccatccgcac
1681 catcctggaa tatgctgaca aagtcttcac ctatatcttc atcctggaga tgttgctcaa
1741 gtggacagcc tatggcttcg tcaagttctt caccaatgcc tggtgttggc tggacttcct
1801 cattgtggct gtctctttag tcagccttat agctaatgcc ctgggctact cggaactagg
1861 tgccataaag tcccttagga ccctaagagc tttgagaccc ttaagagcct tatcacgatt
1921 tgaagggatg agggtggtgg tgaatgcctt ggtgggcgcc atcccctcca tcatgaatgt
1981 gctgctggtg tgtctcatct tctggctgat tttcagcatc atgggagtta acttgtttgc
2041 gggaaagtac cactactgct ttaatgagac ttctgaaatc cgatttgaaa ttgaagatgt
2101 caacaataaa actgaatgtg aaaagcttat ggaggggaac aatacagaga tcagatggaa
2161 gaacgtgaag atcaactttg acaatgttgg ggcaggatac ctggcccttc ttcaagtagc
2221 aaccttcaaa ggctggatgg acatcatgta tgcagctgta gattcccgga agcctgatga
2281 gcagcctaag tatgaggaca atatctacat gtacatctat tttgtcatct tcatcatctt
2341 cggctccttc ttcaccctga acctgttcat tggtgtcatc attgataact tcaatcaaca
2401 aaagaaaaag ttcggaggtc aggacatctt catgaccgaa gaacagaaga agtactacaa
2461 tgccatgaaa aagctgggct caaagaagcc acagaaacct attccccgcc ccttgaacaa
2521 aatccaagga atcgtctttg attttgtcac tcagcaagcc tttgacattg ttatcatgat
2581 gctcatctgc cttaacatgg tgacaatgat ggtggagaca gacactcaaa gcaagcagat
2641 ggagaacatc ctctactgga ttaacctggt gtttgttatc ttcttcacct gtgagtgtgt
2701 gctcaaaatg tttgcgttga ggcactacta cttcaccatt ggctggaaca tcttcgactt
2761 cgtggtagtc atcctctcca ttgtgggaat gttcctggca gatataattg agaaatactt
2821 tgtttcccca accctattcc gagtcatccg attggcccgt attgggcgca tcttgcgtct
2881 gatcaaaggc gccaaaggga ttcgtaccct gctctttgcc ttaatgatgt ccttgcctgc
2941 cctgttcaac atcggccttc tgctcttcct ggtcatgttc atcttctcca tttttgggat
3001 gtccaatttt gcatatgtga agcacgaggc tggtatcgat gacatgttca actttgagac
3061 atttggcaac agcatgatct gcctgtttca aatcacaacc tcagctggtt gggatggcct
3121 gctgctgccc atcctaaacc gcccccctga ctgcagccta gataaggaac acccagggag
3181 tggctttaag ggagattgtg ggaacccctc agtgggcatc ttcttctttg taagctacat
3241 catcatctct ttcctaattg tcgtgaacat gtacattgcc atcatcctgg agaacttcag
3301 tgtagccaca gaggaaagtg cagaccctct gagtgaggat gactttgaga ccttctatga
3361 gatctgggag aagttcgacc ccgatgccac ccagttcatt gagtactgta agctggcaga
3421 ctttgcagat gccttggagc atcctctccg agtgcccaag cccaatacca ttgagctcat
3481 cgctatggat ctgccaatgg tgagcgggga tcgcatccac tgcttggaca tcctttttgc
3541 cttcaccaag cgggtcctgg gagatagcgg ggagttggac atcctgcggc agcagatgga
3601 agagcggttc gtggcatcca atccttccaa agtgtcttac gagccaatca caaccacact
3661 gcgtcgcaag caggaggagg tatctgcagt ggtcctgcag cgtgcctacc ggggacattt
3721 ggcaaggcgg ggcttcatct gcaaaaagac aacttctaat aagctggaga atggaggcac
3781 acaccgggag aaaaaagaga gcaccccatc tacagcctcc ctcccgtcct atgacagtgt
3841 aactaaacct gaaaaggaga aacagcagcg ggcagaggaa ggaagaaggg aaagagccaa
3901 aagacaaaaa gaggtcagag aatccaagtg ttgtacagac tacaaagacc atgacggtga
3961 ttataaagat catgacatcg actacaagga tgacgatgac aagtaactcg agataatcaa
4021 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
4081 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
4141 ttcattttct cctccttgta taaatcctgg ttagttcttg ccacggcgga actcatcgcc
4201 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
4261 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccatctagct ttatttgtga
4321 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
4381 caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag
4441 atctggagac cggtgtggca cgtgcggacc gagcggccgc aggaacccct agtgatggag
4501 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
4561 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag
4621 gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
4681 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
4741 cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc
4801 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
4861 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
4921 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
4981 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct
5041 attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga
5101 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
5161 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
5221 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
5281 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
5341 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
5401 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
5461 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
5521 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
5581 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
5641 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
5701 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
5761 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
5821 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
5881 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
5941 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
6001 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
6061 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
6121 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
6181 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
6241 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
6301 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
6361 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
6421 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
6481 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
6541 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
6601 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
6661 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
6721 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
6781 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
6841 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
6901 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
6961 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
7021 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
7081 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
7141 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
7201 ggccttttgc tcacatgt
//
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