| 质粒名称: | pKSEe401R |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 218532 |
| 存储实验室: | Kirill Demchenko |
|
载体骨架基本信息 |
|
| 载体名称: | pKSE401 |
| 载体出品公司: | Qi-Jun Chen (Addgene plasmid # 62202) |
| 空载体大小: | 18202bp |
| 完整质粒大小: | 18202bp |
| 载体类型: | 植物表达 |
| 筛选标记: | Neomycin (select with G418) ; pAtUBQ10-DsRED1 |
| 载体抗性: | Kanamycin and Spectinomycin, 50 & 50 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 克隆方法: | Golden Gate |
| 5’测序引物 | 35S prom: CTATCCTTCGCAAGACCCTTC; M13/pUC For: CCCAGTCACGACGTTGTAAAACG; DsRed1-N: GTACTGGAACTGGGGGGACAG |
| 3’测序引物 | M13 Rev: CAGGAAACAGCTATGAC; DsRed1-C: AGCTGGACATCACCTCCCACAACG |
|
文献引用方法 |
|
| 材料和方法部分: | pKSEe401R was a gift from Kirill Demchenko (Addgene plasmid # 218532 ; http://n2t.net/addgene:218532 ; RRID:Addgene_218532) |
| 参考文献部分: | Do DEEPER ROOTING 1 Homologs Regulate the Lateral Root Slope Angle in Cucumber (Cucumis sativus)?. Kiryushkin AS, Ilina EL, Kiikova TY, Pawlowski K, Demchenko KN. Int J Mol |
买家导航
pKSEe401R载体质粒基本信息
pKSEe401R质粒图谱载体图谱和pKSEe401R载体序列质粒序列多克隆位点信息
pKSEe401R质粒载体简介
pKSEe401R质粒序列载体序列
LOCUS pKSEe401R 18202 bp DNA circular SYN 15-DEC-2025
DEFINITION CRISPR/Cas9 genome editing vector pKSEe401R contains the maize
codon-optimized Cas9 (zCas9) enhanced by the 5′-UTR fragment of
OsMac3 and the pAtUBQ10-DsRED1-NOSt screening cassette in the
backbone..
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 18202)
AUTHORS Kiryushkin AS, Ilina EL, Kiikova TY, Pawlowski K, Demchenko KN
TITLE Do DEEPER ROOTING 1 Homologs Regulate the Lateral Root Slope Angle
in Cucumber (Cucumis sativus)?
JOURNAL Int J Mol Sci. 2024 Feb 6;25(4):1975. doi: 10.3390/ijms25041975.
PUBMED 38396652
REFERENCE 2 (bases 1 to 18202)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol
Sci."; date: "2024-02-6"; volume: "25(4):1975. doi"; pages: "
10.3390/ijms25041975"
COMMENT Sequence Label: pKSEe401R
FEATURES Location/Qualifiers
source 1..18202
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 21..45
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
primer_bind 233..255
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 247..264
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 248..264
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
CDS 977..1768
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
misc_RNA 1920..1995
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
promoter 2609..2953
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
CDS 3265..3330
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 3337..3357
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 3382..7482
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 3382..7482
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
RBS 7395..7403
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
CDS 7483..7530
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
terminator complement(8181..8433)
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
CDS complement(join(8446..9120,9121..9123,9124..9126))
/codon_start=1
/product="wild-type DsRed"
/label=DsRed1
/note="mammalian codon-optimized"
/translation="MARSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
HLFL"
primer_bind complement(8508..8531)
/label=DsRed1-C
/note="DsRed1, forward primer"
primer_bind complement(8706..8725)
/label=mCherry-F
/note="mCherry, forward primer"
primer_bind 8923..8943
/label=DsRed1-N
/note="DsRed1, reverse primer"
primer_bind complement(9793..9809)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(9793..9809)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(9806..9828)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 9817..9833
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(9841..9847,9848..9865,9866..9871))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9886..9907
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 10098..10774
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
CDS 10837..11634
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(10894..10913)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 11504..11523
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 11691..11865
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
misc_feature 11943..11967
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
CDS 12392..13186
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
rep_origin 13273..13861
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 13762..13781
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 14015..14032
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 14047..14187
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(14113..14132)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 14273..14295
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
rep_origin 14531..14725
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
CDS complement(14791..15864)
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
CDS complement(16293..16922)
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
1 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta
61 aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc
121 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
181 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
241 cgacgttgta aaacgacggc cagtgccaag cttcgacttg ccttccgcac aatacatcat
301 ttcttcttag ctttttttct tcttcttcgt tcatacagtt tttttttgtt tatcagctta
361 cattttcttg aaccgtagct ttcgttttct tctttttaac tttccattcg gagtttttgt
421 atcttgtttc atagtttgtc ccaggattag aatgattagg catcgaacct tcaagaattt
481 gattgaataa aacatcttca ttcttaagat atgaagataa tcttcaaaag gcccctggga
541 atctgaaaga agagaagcag gcccatttat atgggaaaga acaatagtat ttcttatata
601 ggcccattta agttgaaaac aatcttcaaa agtcccacat cgcttagata agaaaacgaa
661 gctgagttta tatacagcta gagtcgaagt agtgattggg agaccaaccc agtggacata
721 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
781 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
841 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
901 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
961 aacaaagtta aacatcatgg gggaagcggt gatcgccgaa gtatcgactc aactatcaga
1021 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
1081 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
1141 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
1201 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
1261 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
1321 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
1381 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
1441 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
1501 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
1561 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
1621 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
1681 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
1741 cgagatcacc aaggtagtcg gcaaataatg tctagctaga aattcgttca agccgacgcc
1801 gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa gcacataatt gctcacagcc
1861 aaactatcag gtcaagtctg cttttattat ttttaagcgt gcataataag ccggtctcgg
1921 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
1981 gcaccgagtc ggtgcttttt tttgcaaaat tttccagatc gatttcttct tcctctgttc
2041 ttcggcgttc aatttctggg gttttctctt cgttttctgt aactgaaacc taaaatttga
2101 cctaaaaaaa atctcaaata atatgattca gtggttttgt acttttcagt tagttgagtt
2161 ttgcagttcc gatgagataa accaataagc ttgcatgcct gcaggtcaac atggtggagc
2221 acgacacact tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa
2281 ttgagacttt tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta
2341 tctgtcactt tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt
2401 gcgataaagg aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac
2461 ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag
2521 tggattgatg tgataacatg gtggagcacg acacacttgt ctactccaaa aatatcaaag
2581 atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa
2641 acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg
2701 aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct
2761 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag
2821 acgttccaac cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg
2881 atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc
2941 atttggagag gacctcgacc tcaacacaac atatacaaaa caaacgaatc tcaagcaatc
3001 aagcattcta cttctattgc agcaatttaa atcatttctt ttaaagcaaa agcaattttc
3061 tgaaaatttt caccatttac gaacgatact cgagtaatct agaaagacta aagagagctt
3121 tttcatacca aagaagtaca acaaaagatt tgctcctcat tttctgaatc ctgggactct
3181 ctagcctgta gaagaagaaa ggcaggaatt tcagctcaag agaacagatc acaatattta
3241 cccacggcac tgtctcgcaa tatggattac aaggaccacg acggggatta caaggaccac
3301 gacattgatt acaaggatga tgatgacaag atggctccga agaagaagag gaaggttggc
3361 atccacgggg tgccagctgc tgacaagaag tactcgatcg gcctcgatat tgggactaac
3421 tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa gttcaaggtc
3481 ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct cctgttcgac
3541 agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg gtacacgcgc
3601 aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc gaaggttgac
3661 gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa gaagcacgag
3721 cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa gtaccctacg
3781 atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct ccgcctgatc
3841 tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga gggggatctc
3901 aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac gtacaaccag
3961 ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat cctgtccgct
4021 aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg cgagaagaag
4081 aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa cttcaagtcg
4141 aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata cgacgatgac
4201 ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct cgctgccaag
4261 aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga gattacgaag
4321 gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga tctgaccctc
4381 ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt cttcgatcag
4441 tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga gttctacaag
4501 ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt gaagctcaat
4561 cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc acaccagatt
4621 catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctatcc tttcctcaag
4681 gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta cgtcggccca
4741 ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga gacgatcacg
4801 ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt catcgagagg
4861 atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca ctcgctcctg
4921 tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac cgagggcatg
4981 aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct cctgttcaag
5041 accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa gattgagtgc
5101 ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct ggggacctac
5161 cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga gaatgaggat
5221 atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat gatcgaggag
5281 cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct caagaggagg
5341 aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg ggacaagcag
5401 tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg caatttcatg
5461 cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc tcaggtgagc
5521 ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc agctatcaag
5581 aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat gggcaggcac
5641 aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca gaagggccag
5701 aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct ggggtcccag
5761 atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct ctacctgtac
5821 tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa caggctcagc
5881 gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc cattgacaac
5941 aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc ttcagaggag
6001 gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct gatcacgcag
6061 cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct ggacaaggcg
6121 ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt tgcgcagatt
6181 ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg cgaggtgaag
6241 gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca gttctacaag
6301 gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc ggtggtcggc
6361 acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg ggactacaag
6421 gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa ggctaccgcc
6481 aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac tctggccaat
6541 ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga gatcgtgtgg
6601 gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca agtgaatatc
6661 gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct gcctaagcgg
6721 aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta cggcgggttc
6781 gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa gggcaagtcc
6841 aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg ctccagcttc
6901 gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa gaaggacctg
6961 atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa gcggatgctg
7021 gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa gtatgtgaac
7081 ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga caacgagcag
7141 aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga gcagatttcc
7201 gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct ctccgcgtac
7261 aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca tctcttcacc
7321 ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat cgatcgcaag
7381 aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc gattaccggc
7441 ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc agcggcgacg
7501 aagaaggcgg ggcaggcgaa gaagaagaag tgagagctca gagctttcgt tcgtatcatc
7561 ggtttcgaca acgttcgtca agttcaatgc atcagtttca ttgcgcacac accagaatcc
7621 tactgagttt gagtattatg gcattgggaa aactgttttt cttgtaccat ttgttgtgct
7681 tgtaatttac tgtgtttttt attcggtttt cgctatcgaa ctgtgaaatg gaaatggatg
7741 gagaagagtt aatgaatgat atggtccttt tgttcattct caaattaata ttatttgttt
7801 tttctcttat ttgttgtgtg ttgaatttga aattataaga gatatgcaaa cattttgttt
7861 tgagtaaaaa tgtgtcaaat cgtggcctct aatgaccgaa gttaatatga ggagtaaaac
7921 acttgtagtt gtaccattat gcttattcac taggcaacaa atatattttc agacctagaa
7981 aagctgcaaa tgttactgaa tacaagtatg tcctcttgtg ttttagacat ttatgaactt
8041 tcctttatgt aattttccag aatccttgtc agattctaat cattgcttta taattatagt
8101 tatactcatg gatttgtagt tgagtatgaa aatatttttt aatgcatttt atgacttgcc
8161 aattgattga caacgaattc gatctagtaa catagatgac accgcgcgcg ataatttatc
8221 ctagtttgcg cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct
8281 aatcataaaa acccatctca taaataacgt catgcattac atgttaatta ttacatgctt
8341 aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct
8401 taagaaactt tattgccaaa tgtttgaacg atcgggaatt ggatcctaca ggaacaggtg
8461 gtggcggccc tcggtgcgct cgtactgctc cacgatggtg tagtcctcgt tgtgggaggt
8521 gatgtccagc ttggagtcca cgtagtagta gccgggcagc tgcacgggct tcttggccat
8581 gtagatggac ttgaactcca ccaggtagtg gccgccgtcc ttcagcttca gggccttgtg
8641 gatctcgccc ttcagcacgc cgtcgcgggg gtacaggcgc tcggtggagg cctcccagcc
8701 catggtcttc ttctgcatta cggggccgtc ggaggggaag ttcacgccga tgaacttcac
8761 cttgtagatg aagcagccgt cctgcaggga ggagtcctgg gtcacggtca ccacgccgcc
8821 gtcctcgaag ttcatcacgc gctcccactt gaagccctcg gggaaggaca gcttcttgta
8881 gtcggggatg tcggcggggt gcttcacgta caccttggag ccgtactgga actgggggga
8941 caggatgtcc caggcgaagg gcagggggcc gcccttggtc accttcagct tcacggtgtt
9001 gtggccctcg taggggcggc cctcgccctc gccctcgatc tcgaactcgt ggccgttcac
9061 ggtgccctcc atgcgcacct tgaagcgcat gaactccttg atgacgttct tggaggagcg
9121 cgccatggca aagatctgca tctgttaatc agaaaaactc agattaatcg acaaattcga
9181 tcgcacaaac tagaaactaa caccagatct agatagaaat cacaaatcga agagtaatta
9241 ttcgacaaaa ctcaaattat ttgaacaaat cggatgatat ctatgaaacc ctaatcgaga
9301 attaagatga tatctaacga tcaaacccag aaaatcgtct tcgatctaag attaacagaa
9361 tctaaaccaa agaacatata cgaaattggg atcgaacgaa aacaaaatcg aagattttga
9421 gagaataagg aacacagaaa tttaccttga tcacggtaga gagaattgag agaaagtttt
9481 taagattttg agaaattgaa atctgaattg tgaagaagaa gagctctttg ggtattgttt
9541 tatagaagaa gaagaagaaa agacgaggac gactaggtca cgagaaagct aaggcggtga
9601 agcaatagct aataataaaa tgacacgtgt attgagcgtt gtttacacgc aaagttgttt
9661 ttggctaatt gccttatttt taggttgagg aaaagtattt gtgctttgag ttgataaaca
9721 cgactcgtgt gtgccggctg caaccacttt gacgccgttt attactggac ttcgtccgga
9781 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
9841 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
9901 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
9961 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattggct agagcagctt
10021 gccaacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga tacagtctca
10081 gaagaccaaa gggctattga gacttttcaa caaagggtaa tatcgggaaa cctcctcgga
10141 ttccattgcc cagctatctg tcacttcatc aaaaggacag tagaaaagga aggtggcacc
10201 tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc tgccgacagt
10261 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc
10321 acgtcttcaa agcaagtgga ttgatgtgaa catggtggag cacgacactc tcgtctactc
10381 caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag
10441 ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag
10501 gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat
10561 cgttcaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
10621 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
10681 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
10741 aggaagttca tttcatttgg agaggacacg ctgaaatcac cagtctctct ctacaaatct
10801 atctctctcg agctttcgca gatctgtcga tcgaccatgg ggattgaaca agatggattg
10861 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
10921 acaatcggct gctctgatgc cgccgtgttt cggctgtcag cgcaggggcg cccggttctt
10981 tttgtcaaga ccgacctgtc cggtgccctg aatgaactcc aggacgaggc agcgcggcta
11041 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
11101 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
11161 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
11221 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
11281 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
11341 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgaca
11401 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
11461 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
11521 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
11581 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
11641 ctctggggtt cggatcgatc ctctagctag agtcgatcga caagctcgag tttctccata
11701 ataatgtgtg agtagttccc agataaggga attagggttc ctatagggtt tcgctcatgt
11761 gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa
11821 aatttctaat tcctaaaacc aaaatccagt actaaaatcc agatcccccg aattaattcg
11881 gcgttaattc agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat
11941 ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc
12001 ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga
12061 gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact
12121 aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat
12181 gacagagcgt tgctgcctgt gatcaccgcg gtttcaaaat cggctccgtc gatactatgt
12241 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
12301 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
12361 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
12421 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
12481 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
12541 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
12601 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
12661 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
12721 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
12781 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
12841 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
12901 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
12961 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
13021 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
13081 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
13141 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagaatgcat
13201 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
13261 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
13321 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
13381 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
13441 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
13501 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
13561 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
13621 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
13681 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
13741 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
13801 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
13861 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
13921 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
13981 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
14041 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
14101 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta
14161 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc
14221 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
14281 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg
14341 tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta gattgcctgg
14401 ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg aagcggcggg
14461 gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg
14521 gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt
14581 ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt gtgaccggtt
14641 cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt acggctttgg
14701 gttcccaatg tacgtgctat ccacaggaaa cagacctttt cgaccttttt cccctgctag
14761 ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt cgccctcgat
14821 caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc
14881 gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga acttcttgaa
14941 ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg cttctgcctt
15001 gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat cgatcaaaaa
15061 gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct cgcggtacat
15121 ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct ttacgatctt
15181 gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt tcttggcctt
15241 cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc
15301 gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg caacgtgtgg
15361 acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca tggattcggt
15421 tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca tgccggccgg
15481 ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct cgccagctcg
15541 tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt
15601 gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt
15661 cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc
15721 ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc
15781 ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga tttgtaccgg
15841 gccggatggt ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc agtgccattg
15901 cagggccggc aggcaaccca gccgcttacg cctggccaac cgcccgttcc tccacacatg
15961 gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc tcctttagcc
16021 gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct gcgcgatgta
16081 ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt
16141 gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct
16201 ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt
16261 gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt aatttcagcg
16321 gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg
16381 ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc aagaatgggc
16441 agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc
16501 cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc
16561 agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac cggaatcagc
16621 acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg
16681 atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg
16741 atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc
16801 tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc gcgggctaga
16861 tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc gataaccttc
16921 atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca gcgaccgcat
16981 gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc tcggtttctt
17041 cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg gccaggattt
17101 catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca
17161 tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt
17221 tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa
17281 tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg
17341 ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg cctctttcac
17401 ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt
17461 agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg
17521 gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca acaccatgcg
17581 gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc
17641 ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag
17701 cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg ccagctccgg
17761 gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg
17821 cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag
17881 caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt cgcaagtatt
17941 ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc
18001 ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg
18061 tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt gatcccgagg
18121 ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt ttcacgccct
18181 tttaaatatc cgattattct aa
//
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