pAAVLINKv1-pCALM1-Cre-3'-RIMS2-FLAG

LOCUS       pAAVLINKv1-pCALM1-Cre-3'-RIMS2-FLAG                6421 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6421)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..3134
                     /codon_start=1
                     /label=RIMS2
                     /translation="GLRGTRTMTGHYNTISRMDRHRVMDDHYSPDRDRDCEAADRQPYH
                     RSRSTEQRPLLERTTTRSRSTERPDTNLMRSMPSLMTGRSAPPSPALSRSHPRTGSVQT
                     SPSSTPVAGRRGRQLPQLPPKGTLDRKELDSTRRRYAGTMDIEERNRQMKINKYKQVAG
                     SDPRLEQDYHSKYRSGWDPHRGADNVSTKSSDSDVSDISAVSRTSSASRFSSTSYMSVQ
                     SERPGGNKKISVFTSKMQSRQMGISGKNMTKSTSISGDMCSLEKNDGSQSDTAVGTLGT
                     SGKKRRSSLGAKMVAIVGLSRKSRSASQLSQTEAGGKKLRSTVQRSTETGLAVEMRNWM
                     TRQASRESTDGSMNSYSSEGNLIFPGVRLASDSQFSDFLDGLGPAQLVGRQTLATPAMG
                     DIQVGMMDKKGQLEVEIIRARGLVVKPGSKTLPAPYVKVYLLDNGVCIAKKKTKVARKT
                     LEPLYQQLLSFEESPQGKVLQIIVWGDYGRMDHKSFMGVAQILLDELELSNMVIGWFKL
                     FPPSSLVDPTLAPLTRRASQSSLESSTGPSYSRS"
     misc_feature    1740..1745
                     /label=SDS
     misc_feature    2159..2164
                     /label=SDS
     misc_feature    2426..2431
                     /label=SDS
     CDS             3141..3206
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3210..3641
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3354..3359
                     /label=SDS
     repeat_region   3684..3824
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3684..3813
                     /label=AAV2 ITR
     rep_origin      3899..4354
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4636..4740
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4741..5601
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5772..6360
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca ggggcttcga gggacccgca
     1501 ctatgaccgg acattataat acaattagcc gaatggacag acatcgtgtc atggatgacc
     1561 attattctcc agatagagac agggattgtg aagcagcaga tagacagcca tatcacagat
     1621 ccagatcaac agaacaacgg cctctccttg agcggaccac cacccgctcc agatccactg
     1681 aacgtcctga tacaaacctc atgaggtcga tgccttcatt aatgactgga agatctgccc
     1741 ctccttcacc tgccttatcg aggtctcatc ctcgtactgg gtctgtccag acaagcccat
     1801 caagtactcc agtcgcagga cgaaggggcc gacagcttcc acagcttcca ccaaagggaa
     1861 cgttggatag aaaagaactg gactctacaa ggagaagata tgcaggtact atggatatag
     1921 aggagagaaa tcgccaaatg aaaattaaca aatacaaaca ggtagccgga tcagatccca
     1981 gactggaaca agattaccat tcgaagtatc gatcaggatg ggatcctcat agaggggcag
     2041 ataatgtttc tactaaatct tcggacagtg atgtaagtga tatatctgcg gtttcaagga
     2101 ctagtagtgc ttctcgtttc agcagcacaa gctacatgtc tgtccaatca gaacgcccag
     2161 gaggaaacaa gaaaatcagt gtctttacat ccaaaatgca aagcagacaa atgggcatat
     2221 cagggaagaa catgacaaaa agcaccagca tcagtggaga catgtgctca ctggagaaga
     2281 atgatggcag ccagtctgac actgcagtgg gcaccttggg caccagtggc aaaaagcggc
     2341 gctctagcct tggtgccaaa atggtagcta tcgttggtct gtcacggaaa agtcgcagtg
     2401 cttctcagct cagccaaacg gaagcaggag gtaaaaaact aaggagcact gtccaaagaa
     2461 gtacagaaac aggcctggcc gtggaaatga ggaactggat gactcgacag gcaagccgag
     2521 agtctacaga tggtagcatg aacagctaca gctcagaagg aaatctgatt ttccctggtg
     2581 ttcgcttggc ctctgatagc cagttcagtg atttcctgga tggccttggc cctgctcagc
     2641 tagtgggacg ccagactctg gcaacacctg caatgggtga cattcaagta ggaatgatgg
     2701 acaaaaaggg acagctggag gtagaaatca tccgggcccg tggccttgtt gtaaaaccag
     2761 gttccaagac actgccagca ccgtatgtaa aagtgtatct attagataac ggagtctgca
     2821 tagccaaaaa gaaaacaaaa gtggcaagaa aaacgctgga acccctttac cagcagctat
     2881 tatctttcga agagagtcca caaggaaaag ttttacagat catcgtctgg ggagattatg
     2941 gccgcatgga tcacaaatct tttatgggag tggcccagat acttttagat gaactagagc
     3001 tatccaatat ggtgatcgga tggttcaaac ttttcccacc ttcctcccta gtagatccaa
     3061 ccttggctcc tctgacaaga agagcttccc aatcatctct ggaaagttca actggacctt
     3121 cttactctcg ttcatgtaca gactacaaag accatgacgg tgattataaa gatcatgaca
     3181 tcgactacaa ggatgacgat gacaagtaac tcgagataat caacctctgg attacaaaat
     3241 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     3301 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     3361 gtataaatcc tggttagttc ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     3421 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgtttattt gtgaaatttg
     3481 tgatgctatt gctttatttg taaccatcta gctttatttg tgaaatttgt gatgctattg
     3541 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     3601 ttatgtttca ggttcagggg gagatgtggg aggtttttta aagatctgga gaccggtgtg
     3661 gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
     3721 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     3781 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
     3841 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
     3901 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     3961 acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4021 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4081 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     4141 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4201 ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa
     4261 gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4321 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
     4381 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     4441 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     4501 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     4561 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     4621 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4681 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4741 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4801 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4861 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4921 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4981 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5041 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5101 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5161 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5221 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5281 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5341 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5401 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5461 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5521 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5581 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5641 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5701 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5761 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5821 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5881 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     5941 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6001 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6061 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6121 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6181 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6241 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6301 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6361 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6421 t
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