| 质粒名称: | mTert-pBabe-puro |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 36413 |
| 存储实验室: | Marta Alvarez, Joseph Bidwell |
|
载体骨架基本信息 |
|
| 载体名称: | pBABE-puro |
| 载体出品公司: | Bob Weinberg (Addgene Plasmid #1764) |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5169 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | mTERT |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | BC127068.1 |
| 启动子 | LTR |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI（未销毁） |
| 3' 克隆位点: | EcoRI（未销毁） |
| 5' 测序引物 | mTERT 1: 5'-TTCGAACAGCAAACCAACAG-3' |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | mTert-pBabe-puro was a gift from Marta Alvarez & Joseph Bidwell (Addgene plasmid # 36413 ; http://n2t.net/addgene:36413 ; RRID:Addgene_36413) |
| 参考文献部分: | Immortalization and characterization of osteoblast cell lines generated from wild-type and Nmp4-null mouse bone marrow stromal cells using murine telomerase reverse transcriptase (mTERT). Alvarez MB, Childress P, Philip BK, Gerard-O'Riley R, Hanlon M, Herbert BS, Robling AG, Pavalko FM, Bidwell JP. J Cell Physiol. 2012 May;227(5):1873-82. doi: 10.1002/jcp.22915. 10.1002/jcp.22915 PubMed 21732358 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS mTert-pBabe-puro 7820 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS mTert-pBabe-puro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7820)
AUTHORS Alvarez MB, Childress P, Philip BK, Gerard-O'Riley R, Hanlon M,
Herbert BS, Robling AG, Pavalko FM, Bidwell JP
TITLE Immortalization and characterization of osteoblast cell lines
generated from wild-type and Nmp4-null mouse bone marrow stromal
cells using murine telomerase reverse transcriptase (mTERT).
JOURNAL J Cell Physiol. 2012 May;227(5):1873-82. doi: 10.1002/jcp.22915.
PUBMED 21732358
REFERENCE 2 (bases 1 to 7820)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7820
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 8..477
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 542..741
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 942..1358
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1288..1310
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1326..1342
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
primer_bind complement(4150..4170)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 4155..4484
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4335..4470
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4397..4416
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4494..5093
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(4494..4513)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 4990..5010
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 5314..5783
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(5913..5930)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6084..6672)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6164..6183)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6832..7692)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7455..7474
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7693..7797)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatccca gtgtggtggt
1381 acgtaggaat tcgcccttca tcccggcctt gagcacaatg acccgcgctc ctcgttgccc
1441 cgcggtgcgc tctctgctgc gcagccgata ccgggaggtg tggccgctgg caacctttgt
1501 gcggcgcctg gggcccgagg gcaggcggct tgtgcaaccc ggggacccga agatctaccg
1561 cactttggtt gcccaatgcc tagtgtgcat gcactggggc tcacagcctc cacctgccga
1621 cctttccttc caccaggtgt catccctgaa agagctggtg gccagggttg tgcagagact
1681 ctgcgagcgc aacgagagaa acgtgctggc ttttggcttt gagctgctta acgaggccag
1741 aggcgggcct cccatggcct tcactagtag cgtgcgtagc tacttgccca acactgttat
1801 tgagaccctg cgtgtcagtg gtgcatggat gctactgttg agccgagtgg gcgacgacct
1861 gctggtctac ctgctggcac actgtgctct ttatcttctg gtgcccccca gctgtgccta
1921 ccaggggaga tggccaagag cgtctaaacc cctcattcct actcagcaac ctccagccta
1981 acttgactgg ggccaggaga ctggtggaga tcatctttct gggctcaagg cctaggacat
2041 caggaccact ctgcaggaca caccgtctat cgcgtcgata ctggcagatg cggcccctgt
2101 tccaacagct gctggtgaac catgcagagt gccaatatgt cagactcctc aggtcacatt
2161 gcaggtttcg aacagcaaac caacaggtga cagatgcctt gaacaccagc ccaccgcacc
2221 tcatggattt gctccgcctg cacagcagtc cctggcaggg aaggaccgtg tccccgctgc
2281 agagcaccgt ctgagggaga ggatcctggc tacgttcctg ttctggctga tggacacata
2341 cgtggtacag ctgcttaggt cattctttta catcacagag agcacattcc agaagaacag
2401 gctcttcttc taccgtaaga gtgtgtggag caagctgcag agcattggag tcaggcaaca
2461 ccttgagaga gtgcggctac gggagctgtc acaagaggag gtcaggcatc accaggacac
2521 ctggctagcc atgcccatct gcagactgcg cttcatcccc aagcccaacg gcctgcggcc
2581 cattgtgaac atgagttata gcatgggtac cagagctttg ggcagaagga agcaggccca
2641 gcatttcacc cagcgtctca agactctctt cagcatgctc aactatgagc ggacaaaaca
2701 tcctcacctt atggggtctt ctgtactggg tatgaatgac atctacagga cctggcgggc
2761 ctttgtgctg cgtgtgcgtg ctctggacca gacacccagg atgtactttg ttaaggcaga
2821 tgtgaccggg gcctatgatg ccatccccca gggtaagctg gtggaggttg ttgccaatat
2881 gatcaggcac tcggagagca cgtactgtat ccgccagtat gcagtggtcc ggagagatag
2941 ccaaggccaa gtccacaagt cctttaggag acaggtcacc accctctctg acctccagcc
3001 atacatgggc cagttcctta agcatctgca ggattcagat gccagtgcac tgaggaactc
3061 cgttgtcatc gagcagagca tctctatgaa tgagagcagc agcagcctgt ttgacttctt
3121 cctgcacttc ctgcgtcaca gtgtcgtaaa gattggtgac aggtgctata cgcagtgcca
3181 gggcatcccc cagggctcca gcctatccac cctgctctgc agtctgtgtt tcggagacat
3241 ggagaacaag ctgtttgctg aggtgcagcg ggatgggttg cttttacgtt ttgttgatga
3301 ctttctgttg gtgacgcctc acttggacca agcaaaaacc ttcctcagca ccctggtcca
3361 tggcgttcct gagtatgggt gcatgataaa cttgcagaag acagtggtga acttccctgt
3421 ggagcctggt accctgggtg gtgcagctcc ataccagctg cctgctcact gcctgtttcc
3481 ctggtgtggc ttgctgctgg acactcagac tttggaggtg ttctgtgact actcaggtta
3541 tgcccagacc tcaattaaga cgagcctcac cttccagagt gtcttcaaag ctgggaagac
3601 catgcggaac aagctcctgt cggtcttgcg gttgaagtgt cacggtctat ttctagactt
3661 gcaggtgaac agcctccaga cagtctgcat caatatatac aagatcttcc tgcttcaggc
3721 ctacaggttc catgcatgtg tgattcagct tccctttgac cagcgtgtta ggaagaacct
3781 cacattcttt ctgggcatca tctccagcca agcatcctgc tgctatgcta tcctgaaggt
3841 caagaatcca ggaatgacac taaaggcctc tggctccttt cctcctgaag ccgcacattg
3901 gctctgctac caggccttcc tgctcaagct ggctgctcat tctgtcatct acaaatgtct
3961 cctgggacct ctgaggacag cccaaaaact gctgtgccgg aagctcccag aggcgacaat
4021 gaccatcctt aaagctgcag ctgacccagc cctaagcaca gactttcaga ccattttgga
4081 ctaaccctgt ctccttccgc tagatgagag tgcgaagggc gaattcgcca gcacagtggt
4141 cgaccctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
4201 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
4261 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
4321 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
4381 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
4441 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt accatgaccg
4501 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
4561 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
4621 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
4681 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
4741 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
4801 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
4861 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
4921 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
4981 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
5041 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc
5101 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg ataaaataaa
5161 agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca
5221 agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaga
5281 agttcagatc aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg
5341 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc
5401 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc
5461 cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc
5521 ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct
5581 tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg
5641 gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg
5701 cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac
5761 tacccgtcag cgggggtctt tcacatgcag catgtatcaa aattaatttg gttttttttc
5821 ttaagtattt acattaaatg gccatagttg cattaatgaa tcggccaacg cgcggggaga
5881 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
5941 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
6001 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
6061 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
6121 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
6181 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
6241 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
6301 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
6361 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
6421 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
6481 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
6541 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
6601 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
6661 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
6721 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
6781 ttgcggccgc aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
6841 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
6901 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
6961 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
7021 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
7081 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
7141 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
7201 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
7261 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
7321 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
7381 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
7441 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
7501 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
7561 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
7621 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
7681 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
7741 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
7801 atttccccga aaagtgccac
//
