| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-NRXN1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001450 |
| 产品用途: | 3' AAVLINK plasmid for NRXN1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | NRXN1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-NRXN1-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-NRXN1-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-NRXN1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-NRXN1-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-NRXN1-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-NRXN1-FLAG 7225 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7225)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 8, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7225
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..141
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..141
/label=AAV2 ITR
promoter 156..276
/label=pCALM1
CDS 288..1319
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1320..1360
/label=Splicing Donor
protein_bind 1367..1400
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1401..1492
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1493..3938
/codon_start=1
/label=NRXN1
/translation="GWVEQICL*LFRNRLSWQVL*ERGNGFEL*WEHVYENSAPRSHAY
GG*GCFLTVPIPACIWHSDGNHF*RLC*HPPPGARRRTCETDGQSRLYQD*L*FQQRSR
DSFCWL*PQ**RVAHSACSSAWKKFKVNSG*PTGHDRSNGR*SY*AGVP*HRDWHHHRT
TVSFFCPLQLHWTPAELDI*WNGIH*PV*KWRHRLL*A*CQIWLQEHHSRSCHLQDQIE
LCCLSYLASLHFYASFFPVQDNIPRWINSI*QWGWK*LYCG*IS*RVLTLRV*FGKWC*
PHQRKLK*TSQ*QSVAQRDDIKGHQQPPHCKD*HKNHNANHRRSQELRPQE*LIYRRSS
*RNIQILTKTCTCQRRLSRLPGIS*FKWTASGPHLRCSFLQRTDRERM*RAQHNLPRGL
MFQSRCVLATMGWLQL*L*YDFLQWTTLQ*PWDDIYL*QRWWTNHV*VAS**PTQYTSR
QTGHRF*HCSERSRIGASGQFFRLG*LPRTAYTPGKNWS*V*CWDR*HRH*RIQCNH**
WEIPCSSFHEEWWQCHVAGGQLASDRALPCRKQ**RAPGDC*TANSISTWSSS**MATR
QRASAHNLQ*PSNHNNWRERAGPALPGPALWAVLQWLESSEYGSRKRCQHRHSGKCETG
W*SAFLYDN*VNSHCHAIRDVHINYGDYHDPGY*HSQKRKAPDKRTH*PDHR*HPCGLS
RVSQR**GH*PL*AELRWVSQPNPSRRQRAVSRLSRSDPGVQQHHGYGRWDSSRCRPVH
PYPPLCHVQVQKPG*RLIPCGRESKLHQ*LSTVQWGCCKGETTQQCEKLQQK*EKQG*R
VLC"
misc_feature 2501..2506
/label=SDS
misc_feature 2835..2840
/label=SDS
misc_feature 3758..3763
/label=SDS
CDS 3945..4010
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 4014..4445
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4158..4163
/label=SDS
repeat_region 4488..4628
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4488..4617
/label=AAV2 ITR
rep_origin 4703..5158
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5440..5544
/gene="bla"
/label=AmpR promoter
CDS 5545..6405
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6576..7164
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
541 taaaaaccat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac agggatgggt
1501 ggaacagata tgtctgtgat tgttccggaa caggctatct tggcaggtcc tgtgagagag
1561 aggcaacggt tttgagctat gatgggagca tgtttatgaa aattcagctc cccgtagtca
1621 tgcatacgga ggctgaggat gtttccttac ggttccgatc ccagcgtgca tatggcattc
1681 tgatggcaac cacttctaga gactctgctg acaccctccg cctggagcta gacgcaggac
1741 gtgtgaaact gacggtcaat ctagattgta tcaggattaa ctgtaattcc agcaaaggtc
1801 ccgagactct ttttgctggc tataacctca atgataacga gtggcacaca gtgcgtgtag
1861 ttcggcgtgg aaaaagttta aagttaacag tggatgacca acaggccatg acaggtcaaa
1921 tggcaggtga tcatactagg ctggagttcc ataacataga gactggcatc atcacagaac
1981 gacggtatct ttcttctgtc ccctccaact tcattggaca cctgcagagc ttgacattta
2041 atggaatggc atacattgac ctgtgtaaaa atggcgacat agattactgt gagcttaatg
2101 ccagatttgg cttcaggaac atcatagcag atcctgtcac cttcaagacc aaatcgagct
2161 atgttgcctt agctaccttg caagcctaca cttctatgca tctttttttc cagttcaaga
2221 caacatccct agatggatta attctatata acagtgggga tggaaatgac tttattgtgg
2281 ttgaattagt taaagggtac ttacattacg tgtttgattt gggaaatggt gctaacctca
2341 tcaaaggaag ctcaaataaa cctctcaatg acaatcagtg gcacaacgtg atgatatcaa
2401 gggacaccag caacctccac actgtaaaga ttgacacaaa aatcacaacg caaatcaccg
2461 ccggagccag gaacttagac ctcaagagtg acttatatat aggaggagta gctaaagaaa
2521 catacaaatc cttaccaaaa cttgtacatg ccaaagaagg ctttcaaggc tgcctggcat
2581 cagttgattt aaatggacgg cttccggacc tcatctccga tgctcttttc tgcaacggac
2641 agatcgagag aggatgtgaa gggcccagca caacctgcca agaggactca tgttccaatc
2701 aaggtgtgtg cttgcaacaa tgggatggct tcagctgtga ctgtagtatg acttccttca
2761 gtggaccact ctgcaatgac cctgggacga catatatctt tagcaaaggt ggtggacaaa
2821 tcacgtataa gtggcctcct aatgaccgac ccagtacacg agcagacaga ctggccatag
2881 gttttagcac tgttcagaaa gaagccgtat tggtgcgagt ggacagttct tcaggcttgg
2941 gtgactacct agaactgcat atacaccagg gaaaaattgg agttaagttt aatgttggga
3001 cagatgacat cgccattgaa gaatccaatg caatcattaa tgatgggaaa taccatgtag
3061 ttcgtttcac gaggagtggt ggcaatgcca cgttgcaggt ggacagctgg ccagtgatcg
3121 agcgctaccc tgcaggaaac aatgataacg agcgcctggc gattgctaga cagcgaattc
3181 catatcgact tggtcgagta gttgatgaat ggctactcga caaagggcgt cagctcacaa
3241 tcttcaatag ccaagcaacc ataataattg gcgggaaaga gcagggccag cccttccagg
3301 gccagctctc tgggctgtac tacaatggct tgaaagttct gaatatggca gccgaaaacg
3361 atgccaacat cgccatagtg ggaaatgtga gactggttgg tgaagtgcct tcctctatga
3421 caactgagtc aacagccact gccatgcaat cagagatgtc cacatcaatt atggagacta
3481 ccacgaccct ggctactagc acagccagaa gaggaaagcc cccgacaaaa gaacccatta
3541 gccagaccac agatgacatc cttgtggcct cagcagagtg tcccagcgat gatgaggaca
3601 ttgacccctg tgagccgagc tcaggtgggt tagccaaccc aacccgagca ggcggcagag
3661 agccgtatcc aggctcagca gaagtgatcc gggagtccag cagcaccacg ggtatggtcg
3721 ttgggatagt agccgctgcc gccctgtgca tccttatcct cctctatgcc atgtacaagt
3781 acagaaaccg ggatgaaggc tcataccatg tggacgagag tcgaaactac atcagtaact
3841 cagcacagtc caatggggct gttgtaaagg agaaacaacc cagcagtgcg aaaagctcca
3901 acaaaaataa gaaaaacaag gataaagagt attatgtctg tacagactac aaagaccatg
3961 acggtgatta taaagatcat gacatcgact acaaggatga cgatgacaag taactcgaga
4021 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
4081 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
4141 tatggctttc attttctcct ccttgtataa atcctggtta gttcttgcca cggcggaact
4201 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
4261 cgtggtgttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca tctagcttta
4321 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
4381 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt
4441 tttaaagatc tggagaccgg tgtggcacgt gcggaccgag cggccgcagg aacccctagt
4501 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa
4561 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg
4621 cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
4681 catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
4741 gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct
4801 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
4861 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
4921 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
4981 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc
5041 tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat
5101 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta
5161 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
5221 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
5281 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
5341 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
5401 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
5461 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
5521 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
5581 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
5641 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
5701 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
5761 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
5821 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
5881 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
5941 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
6001 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
6061 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
6121 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
6181 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
6241 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
6301 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
6361 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
6421 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
6481 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
6541 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
6601 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
6661 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
6721 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
6781 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
6841 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
6901 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
6961 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
7021 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
7081 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
7141 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
7201 ttttgctggc cttttgctca catgt
//
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