| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-CLTCL1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001513 |
| 产品用途: | 3' AAVLINK plasmid for CLTCL1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CLTCL1 |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-CLTCL1-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-CLTCL1-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-CLTCL1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-CLTCL1-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-CLTCL1-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-CLTCL1-FLAG 6439 bp DNA circular SYN 12-JAN-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6439)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 13, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6439
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3152
/codon_start=1
/label=CLTCL1
/translation="VLIEHIGNLDRAYEFAERCNEPAVWSQLAQAQLQKDLVKEAINSY
IRGDDPSSYLEVVQSASRSNNWEDLVKFLQMARKKGRESYIETELIFALAKTSRVSELE
DFINGPNNAHIQQVGDRCYEEGMYEAAKLLYSNVSNFARPASTLVHLGEYQAAVDNSRK
ASSTRTWKEVCFACMDGQEFRFAQLCGLHIVIHADELEELMCYYQDRGYFEELILLLEA
ALGLERAHMGMFTELAILYSKFKPQKMLEHLELFWSRVNIPKVLRAAEQAHLWAELVFL
YDKYEEYDNAVLTMMSHPTEAWKEGQFKDIITKVANVELCYRALQFYLDYKPLLINDLL
LVLSPRLDHTWTVSFFSKAGQLPLVKPYLRSVQSHNNKSVNEALNHLLTEEEDYQGLRA
SIDAYDNFDNISLAQQLEKHQLMEFRCIAAYLYKGNNWWAQSVELCKKDHLYKDAMQHA
AESRDAELAQKLLQWFLEEGKRECFAACLFTCYDLLRPDMVLELAWRHNLVDLAMPYFI
QVMREYLSKVDKLDALESLRKQEEHVTEPAPLVFDFDGHE"
misc_feature 1852..1857
/label=SDS
misc_feature 1993..1998
/label=SDS
misc_feature 2392..2397
/label=SDS
misc_feature 2650..2655
/label=SDS
misc_feature 2653..2658
/label=SDS
CDS 3159..3224
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3228..3659
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3372..3377
/label=SDS
repeat_region 3702..3842
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3702..3831
/label=AAV2 ITR
rep_origin 3917..4372
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4654..4758
/gene="bla"
/label=AmpR promoter
CDS 4759..5619
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5790..6378
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtcctgatc gagcacattg
1501 gaaacctgga ccgggcatat gagtttgcgg agagatgcaa tgagcctgct gtgtggagtc
1561 agctggccca agcccagctc cagaaagatt tggtgaagga agccatcaac tcctatatca
1621 gaggggacga cccttcctct tacctggaag ttgttcagtc agccagcagg agcaacaact
1681 gggaggatct agttaaattt ctgcagatgg ccaggaaaaa gggccgtgag tcctatatag
1741 agactgaact tatttttgcc ttggctaaaa ccagccgtgt ttctgagcta gaagatttta
1801 ttaatggacc caacaatgcc cacatccagc aggttggaga ccgctgttac gaggagggaa
1861 tgtacgaggc tgccaagctg ctctatagca atgtttctaa ctttgcccgc ccggcttcca
1921 ccttggttca cctcggtgag tatcaggcag cagtggacaa cagccgcaag gccagcagca
1981 cccggacgtg gaaggaggtg tgctttgcct gcatggatgg acaagagttc cgcttcgcac
2041 agctgtgtgg tcttcacatc gtcattcatg cagatgagct ggaggagctg atgtgctatt
2101 accaggatcg tggctacttt gaggagctga tcttgctgtt ggaagcggcc ctgggcctgg
2161 agcgggccca catgggcatg ttcactgagc tggccatcct ctactccaaa ttcaagccac
2221 agaagatgct ggagcatctg gagcttttct ggtcccgtgt caacatccca aaggtgctga
2281 gggctgcaga gcaggcacac ctgtgggctg agctggtgtt cctctatgac aagtacgagg
2341 agtatgacaa tgctgtgctc accatgatga gccaccccac tgaggcctgg aaggagggtc
2401 agttcaagga catcattacc aaggttgcca acgtcgagct ctgttacaga gccctgcagt
2461 tctatttgga ttacaaacca ctgctcatca atgacctgct gctggtgctt tcaccccggc
2521 tggaccacac ctggacagtc agtttctttt caaaggcagg tcagctgccc ctggtgaagc
2581 cttacctgcg gtcagtccag agccacaaca acaagagtgt gaatgaggca ctcaaccacc
2641 tgctgacaga ggaggaggac tatcagggct taagggcatc tatcgatgcc tatgacaact
2701 ttgacaacat cagcctggct cagcagctgg agaagcatca gctgatggag ttcaggtgca
2761 ttgcggccta tctgtacaag ggcaataact ggtgggccca gagcgtggag ctctgcaaga
2821 aggatcatct ctacaaggat gccatgcagc atgctgcaga gtcgcgggat gctgagctgg
2881 cccagaagtt gctgcagtgg ttcctggagg aaggcaagag ggagtgcttc gcagcttgtc
2941 tcttcacctg ctatgacctg cttcgcccag acatggtgct tgagctggcc tggaggcaca
3001 acctcgtgga cttggccatg ccctacttca tccaggtgat gagggagtac ctgagcaagg
3061 tggacaaact ggatgccttg gagagtctgc gcaagcaaga ggagcatgtg acagagcctg
3121 cccctctcgt gtttgatttt gatgggcatg aatgtacaga ctacaaagac catgacggtg
3181 attataaaga tcatgacatc gactacaagg atgacgatga caagtaactc gagataatca
3241 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
3301 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
3361 tttcattttc tcctccttgt ataaatcctg gttagttctt gccacggcgg aactcatcgc
3421 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
3481 gtttatttgt gaaatttgtg atgctattgc tttatttgta accatctagc tttatttgtg
3541 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
3601 acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa
3661 gatctggaga ccggtgtggc acgtgcggac cgagcggccg caggaacccc tagtgatgga
3721 gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc
3781 ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca
3841 ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
3901 tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
3961 acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt cgctttcttc
4021 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
4081 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat
4141 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
4201 acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc tatctcgggc
4261 tattcttttg atttataagg gattttgccg atttcggtct attggttaaa aaatgagctg
4321 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc
4381 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
4441 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
4501 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
4561 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
4621 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
4681 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
4741 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
4801 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
4861 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
4921 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
4981 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
5041 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
5101 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
5161 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
5221 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
5281 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
5341 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
5401 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
5461 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
5521 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
5581 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
5641 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
5701 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
5761 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
5821 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
5881 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
5941 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
6001 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
6061 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
6121 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
6181 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
6241 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
6301 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
6361 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
6421 tggccttttg ctcacatgt
//
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