| 质粒名称: | pCXLE-EGFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 27082 |
| 存储实验室: | Shinya Yamanaka |
|
载体骨架基本信息 |
|
| 载体名称: | pCXLE |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 10180 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | eGFP |
| 物种来源: | -- |
| 插入大小: | 732 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI(未破坏) |
| 3' 克隆位点: | EcoRI (未破坏) |
| 5' 测序引物 | pCAG-F |
| 3' 测序引物 | WPRE-R |
|
文献引用方法 |
|
| 材料和方法部分: | pCXLE-EGFP was a gift from Shinya Yamanaka (Addgene plasmid # 27082 ; http://n2t.net/addgene:27082 ; RRID:Addgene_27082) |
| 参考文献部分: | A more efficient method to generate integration-free human iPS cells. Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi M, Takahashi J, Saji H, Yamanaka S. Nat Methods. 2011 Apr 3. ():. 10.1038/nmeth.1591 PubMed 21460823 |
买家导航
pCXLEEGFP载体基本信息
pCXLEEGFP载体图谱质粒图谱和多克隆位点信息
pCXLEEGFP载体简介
pCXLEEGFP载体序列
LOCUS pCXLE-EGFP 10911 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Integration-free (episomal) expression of EGFP.
ACCESSION .
VERSION .
KEYWORDS pCXLE-EGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10911)
AUTHORS Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong
H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi
M, Takahashi J, Saji H, Yamanaka S
TITLE A more efficient method to generate integration-free human iPS
cells.
JOURNAL Nat Methods. 2011 Apr 3. ():.
PUBMED 21460823
REFERENCE 2 (bases 1 to 10911)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10911
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(72..89)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(243..831)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(323..342)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(1002..1862)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1625..1644
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1863..1967)
/gene="bla"
/label=AmpR promoter
enhancer 1998..2377
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2379..2655
/label=chicken beta-actin promoter
intron 2656..3672
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 3596..3618
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 3680..3699
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
regulatory 3727..3736
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3733..4452
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(3778..3799)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(4039..4058)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 4386..4407
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 4484..5072
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4537..4557)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4955..4966)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(5162..5181)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_site 5227..5282
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal"
primer_bind complement(5281..5300)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(5643..5659)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5643..5659)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5656..5678)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5667..5683
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5691..5721)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5736..5757
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
protein_bind complement(5846..5879)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
CDS 6340..8265
/codon_start=1
/product="Epstein-Barr nuclear antigen 1, also known as
EBNA-1"
/label=EBNA1
/note="crucial for latent viral infection, and for episomal
amplification of vectors containing the oriP origin (Okita
et al., 2013)"
/translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
GEEGQE"
protein_bind complement(8433..8466)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
rep_origin 8653..10442
/label=oriP
/note="Epstein-Barr virus oriP replication origin (Yates et
al., 2000)"
ORIGIN
1 gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
61 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
121 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
181 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
241 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
301 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
361 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
421 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
481 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
541 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
601 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
661 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
721 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
781 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
841 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
901 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
961 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
1021 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
1081 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
1141 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
1201 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
1261 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
1321 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
1381 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
1441 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
1501 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
1561 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
1621 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
1681 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
1741 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
1801 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
1861 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
1921 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
1981 aaagtgccac ctgggtcgac attgattatt gactagttat taatagtaat caattacggg
2041 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
2101 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
2161 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
2221 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
2281 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
2341 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
2401 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
2461 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
2521 gcggggcgag gggcggggcg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc
2581 gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga
2641 agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc
2701 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg
2761 gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt
2821 ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc
2881 tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc
2941 ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag
3001 gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc
3061 tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg
3121 caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg
3181 ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg
3241 gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc
3301 ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa
3361 tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg
3421 aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag
3481 gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag
3541 cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc
3601 ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc
3661 tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa
3721 gaattcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
3781 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
3841 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
3901 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
3961 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
4021 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
4081 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
4141 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
4201 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
4261 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
4321 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
4381 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
4441 ctgtacaagt aagaattcaa gcttcgggga ctagtcatat gataatcaac ctctggatta
4501 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
4561 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
4621 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
4681 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
4741 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
4801 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
4861 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
4921 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
4981 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
5041 gagtcggatc tccctttggg ccgcctcccc gcatcggtaa attcactcct caggtgcagg
5101 ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag
5161 atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt
5221 ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct
5281 cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt
5341 agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag
5401 gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga
5461 cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa
5521 aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca
5581 tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt ggcgtaatca
5641 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
5701 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
5761 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatctcaatt
5821 ccgatcatat tcaataaccc ttaatataac ttcgtataat gtatgctata cgaagttatt
5881 aggtctgaag aggagtttac gtccagccaa gcttaggatc aattctcatg tttgacagct
5941 tatcatcgat aagctgatcc tcacaggccg cacccagctt ttcttccgtt gccccagtag
6001 catctctgtc tggtgacctt gaagaggaag aggaggggtc ccgagaatcc ccatccctac
6061 cgtccagcaa aaagggggac gaggaatttg aggcctggct tgaggctcag gacgcaaatc
6121 ttgaggatgt tcagcgggag ttttccgggc tgcgagtaat tggtgatgag gacgaggatg
6181 gttcggagga tggggaattt tcagacctgg atctgtctga cagcgaccat gaaggggatg
6241 agggtggggg ggctgttgga gggggcagga gtctgcactc cctgtattca ctgagcgtcg
6301 tctaataaag atgtctattg atctctttta gtgtgaatca tgtctgacga ggggccaggt
6361 acaggacctg gaaatggcct aggagagaag ggagacacat ctggaccaga aggctccggc
6421 ggcagtggac ctcaaagaag agggggtgat aaccatggac gaggacgggg aagaggacga
6481 ggacgaggag gcggaagacc aggagccccg ggcggctcag gatcagggcc aagacataga
6541 gatggtgtcc ggagacccca aaaacgtcca agttgcattg gctgcaaagg gacccacggt
6601 ggaacaggag caggagcagg agcgggaggg gcaggagcag gaggggcagg agcaggagga
6661 ggggcaggag caggaggagg ggcaggaggg gcaggagggg caggaggggc aggagcagga
6721 ggaggggcag gagcaggagg aggggcagga ggggcaggag gggcaggagc aggaggaggg
6781 gcaggagcag gaggaggggc aggaggggca ggagcaggag gaggggcagg aggggcagga
6841 ggggcaggag caggaggagg ggcaggagca ggaggagggg caggaggggc aggagcagga
6901 ggaggggcag gaggggcagg aggggcagga gcaggaggag gggcaggagc aggaggggca
6961 ggaggggcag gaggggcagg agcaggaggg gcaggagcag gaggaggggc aggaggggca
7021 ggaggggcag gagcaggagg ggcaggagca ggaggggcag gagcaggagg ggcaggagca
7081 ggaggggcag gaggggcagg agcaggaggg gcaggagggg caggagcagg aggggcagga
7141 ggggcaggag caggaggagg ggcaggaggg gcaggagcag gaggaggggc aggaggggca
7201 ggagcaggag gggcaggagg ggcaggagca ggaggggcag gaggggcagg agcaggaggg
7261 gcaggagggg caggagcagg aggaggggca ggagcaggag gggcaggagc aggaggtgga
7321 ggccggggtc gaggaggcag tggaggccgg ggtcgaggag gtagtggagg ccggggtcga
7381 ggaggtagtg gaggccgccg gggtagagga cgtgaaagag ccaggggggg aagtcgtgaa
7441 agagccaggg ggagaggtcg tggacgtgga gaaaagaggc ccaggagtcc cagtagtcag
7501 tcatcatcat ccgggtctcc accgcgcagg ccccctccag gtagaaggcc atttttccac
7561 cctgtagggg aagccgatta ttttgaatac caccaagaag gtggcccaga tggtgagcct
7621 gacgtgcccc cgggagcgat agagcagggc cccgcagatg acccaggaga aggcccaagc
7681 actggacccc ggggtcaggg tgatggaggc aggcgcaaaa aaggagggtg gtttggaaag
7741 catcgtggtc aaggaggttc caacccgaaa tttgagaaca ttgcagaagg tttaagagct
7801 ctcctggcta ggagtcacgt agaaaggact accgacgaag gaacttgggt cgccggtgtg
7861 ttcgtatatg gaggtagtaa gacctccctt tacaacctaa ggcgaggaac tgcccttgct
7921 attccacaat gtcgtcttac accattgagt cgtctcccct ttggaatggc ccctggaccc
7981 ggcccacaac ctggcccgct aagggagtcc attgtctgtt atttcatggt ctttttacaa
8041 actcatatat ttgctgaggt tttgaaggat gcgattaagg accttgttat gacaaagccc
8101 gctcctacct gcaatatcag ggtgactgtg tgcagctttg acgatggagt agatttgcct
8161 ccctggtttc cacctatggt ggaaggggct gccgcggagg gtgatgacgg agatgacgga
8221 gatgaaggag gtgatggaga tgagggtgag gaagggcagg agtgatgtaa cttgttagga
8281 gacgccctca atcgtattaa aagccgtgta ttcccccgca ctaaagaata aatccccagt
8341 agacatcatg cgtgctgttg gtgtatttct ggccatctgt cttgtcacca ttttcgtcct
8401 cccaacatgg ggcaattgcc ggaaccctta atataacttc gtataatgta tgctatacga
8461 agttattagg tccctcgaag aggttcacta gcggatctca attgggcata cccatgttgt
8521 cacgtcactc agctccgcgc tcaacacctt ctcgcgttgg aaaacattag cgacatttac
8581 ctggtgagca atcagacatg cgacggcttt agcctggcct ccttaaattc acctaagaat
8641 gggagcaacc agcaggaaaa ggacaagcag cgaaaattca cgcccccttg ggaggtggcg
8701 gcatatgcaa aggatagcac tcccactcta ctactgggta tcatatgctg actgtatatg
8761 catgaggata gcatatgcta cccggataca gattaggata gcatatacta cccagatata
8821 gattaggata gcatatgcta cccagatata gattaggata gcctatgcta cccagatata
8881 aattaggata gcatatacta cccagatata gattaggata gcatatgcta cccagatata
8941 gattaggata gcctatgcta cccagatata gattaggata gcatatgcta cccagatata
9001 gattaggata gcatatgcta tccagatatt tgggtagtat atgctaccca gatataaatt
9061 aggatagcat atactaccct aatctctatt aggatagcat atgctacccg gatacagatt
9121 aggatagcat atactaccca gatatagatt aggatagcat atgctaccca gatatagatt
9181 aggatagcct atgctaccca gatataaatt aggatagcat atactaccca gatatagatt
9241 aggatagcat atgctaccca gatatagatt aggatagcct atgctaccca gatatagatt
9301 aggatagcat atgctatcca gatatttggg tagtatatgc tacccatggc aacattagcc
9361 caccgtgctc tcagcgacct cgtgaatatg aggaccaaca accctgtgct tggcgctcag
9421 gcgcaagtgt gtgtaatttg tcctccagat cgcagcaatc gcgcccctat cttggcccgc
9481 ccacctactt atgcaggtat tccccggggt gccattagtg gttttgtggg caagtggttt
9541 gaccgcagtg gttagcgggg ttacaatcag ccaagttatt acacccttat tttacagtcc
9601 aaaaccgcag ggcggcgtgt gggggctgac gcgtgccccc actccacaat ttcaaaaaaa
9661 agagtggcca cttgtctttg tttatgggcc ccattggcgt ggagccccgt ttaattttcg
9721 ggggtgttag agacaaccag tggagtccgc tgctgtcggc gtccactctc tttccccttg
9781 ttacaaatag agtgtaacaa catggttcac ctgtcttggt ccctgcctgg gacacatctt
9841 aataacccca gtatcatatt gcactaggat tatgtgttgc ccatagccat aaattcgtgt
9901 gagatggaca tccagtcttt acggcttgtc cccaccccat ggatttctat tgttaaagat
9961 attcagaatg tttcattcct acactagtat ttattgccca aggggtttgt gagggttata
10021 ttggtgtcat agcacaatgc caccactgaa ccccccgtcc aaattttatt ctgggggcgt
10081 cacctgaaac cttgttttcg agcacctcac atacacctta ctgttcacaa ctcagcagtt
10141 attctattag ctaaacgaag gagaatgaag aagcaggcga agattcagga gagttcactg
10201 cccgctcctt gatcttcagc cactgccctt gtgactaaaa tggttcacta ccctcgtgga
10261 atcctgaccc catgtaaata aaaccgtgac agctcatggg gtgggagata tcgctgttcc
10321 ttaggaccct tttactaacc ctaattcgat agcatatgct tcccgttggg taacatatgc
10381 tattgaatta gggttagtct ggatagtata tactactacc cgggaagcat atgctacccg
10441 tttagggtta acaagggggc cttataaaca ctattgctaa tgccctcttg agggtccgct
10501 tatcggtagc tacacaggcc cctctgattg acgttggtgt agcctcccgt agtcttcctg
10561 ggcccctggg aggtacatgt cccccagcat tggtgtaaga gcttcagcca agagttacac
10621 ataaaggcaa tgttgtgttg cagtccacag actgcaaagt ctgctccagg atgaaagcca
10681 ctcagtgttg gcaaatgtgc acatccattt ataaggatgt caactacagt cagagaaccc
10741 ctttgtgttt ggtccccccc cgtgtcacat gtggaacagg gcccagttgg caagttgtac
10801 caaccaactg aagggattac atgcactgcc ccgcgaagaa ggggcagaga tgtcgtagtc
10861 aggtttagtt cgtccggggc ggggatcgat cctctagagt cgacgctagc g
//
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pCXLEEGFP载体质粒应用举例
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