| 质粒名称: | pAAVLINK-EFS-HA-5'-MYH9 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001584 |
| 产品用途: | 5' AAVLINK plasmid for MYH9 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EFS |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | MYH9 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EFS-HA-5'-MYH9
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EFS-HA-5'-MYH9载体质粒基本信息
pAAVLINK-EFS-HA-5'-MYH9质粒图谱载体图谱和pAAVLINK-EFS-HA-5'-MYH9载体序列质粒序列多克隆位点信息
pAAVLINK-EFS-HA-5'-MYH9质粒载体简介
pAAVLINK-EFS-HA-5'-MYH9质粒序列载体序列
LOCUS pAAVLINK-EFS-HA-5'-MYH9 7259 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7259)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 19, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7259
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..356
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
CDS 371..397
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 404..4399
/codon_start=1
/label=MYH9
/translation="AQQAADKYLYVDKNFINNPLAQADWAAKKLVWVPSDKSGFEPASL
KEEVGEEAIVELVENGKKVKVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKERY
YSGLIYTYSGLFCVVINPYKNLPIYSEEIVEMYKGKKRHEMPPHIYAITDTAYRSMMQD
REDQSILCTGESGAGKTENTKKVIQYLAYVASSHKSKKDQGELERQLLQANPILEAFGN
AKTVKNDNSSRFGKFIRINFDVNGYIVGANIETYLLEKSRAIRQAKEERTFHIFYYLLS
GAGEHLKTDLLLEPYNKYRFLSNGHVTIPGQQDKDMFQETMEAMRIMGIPEEEQMGLLR
VISGVLQLGNIVFKKERNTDQASMPDNTAAQKVSHLLGINVTDFTRGILTPRIKVGRDY
VQKAQTKEQADFAIEALAKATYERMFRWLVLRINKALDKTKRQGASFIGILDIAGFEIF
DLNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIDLIEKP
AGPPGILALLDEECWFPKATDKSFVEKVMQEQGTHPKFQKPKQLKDKADFCIIHYAGKV
DYKADEWLMKNMDPLNDNIATLLHQSSDKFVSELWKDVDRIIGLDQVAGMSETALPGAF
KTRKGMFRTVGQLYKEQLAKLMATLRNTNPNFVRCIIPNHEKKAGKLDPHLVLDQLRCN
GVLEGIRICRQGFPNRVVFQEFRQRYEILTPNSIPKGFMDGKQACVLMIKALELDSNLY
RIGQSKVFFRAGVLAHLEEERDLKITDVIIGFQACCRGYLARKAFAKRQQQLTAMKVLQ
RNCAAYLKLRNWQWWRLFTKVKPLLQVSRQEEEMMAKEEELVKVREKQLAAENRLTEME
TLQSQLMAEKLQLQEQLQAETELCAEAEELRARLTAKKQELEEICHDLEARVEEEEERC
QHLQAEKKKMQQNIQELEEQLEEEESARQKLQLEKVTTEAKLKKLEEEQIILEDQNCKL
AKEKKLLEDRIAEFTTNLTEEEEKSKSLAKLKNKHEAMITDLEERLRREEKQRQELEKT
RRKLEGDSTDLSDQIAELQAQIAELKMQLAKKEEELQAALARVEEEAAQKNMALKKIRE
LESQISELQEDLESERASRNKAEKQKRDLGEELEALKTELEDTLDSTAAQQELRSKREQ
EVNILKKTLEEEAKTHEAQIQEMRQKHSQAVEELAEQLEQTKRVKANLEKAKQTLENER
GELANEVKVLLQGKGDSEHKRKKVEAQLQELQVKFNEGERVRTELADKVTKLQVELDNV
TGLLSQSDSKSSKLTKDFSALESQLQDTQELLQEENRQKLSLSTKLKQ"
misc_feature 540..545
/label=SDS
misc_feature 543..548
/label=SDS
misc_feature 1734..1739
/label=SDS
misc_feature 1866..1871
/label=SDS
misc_feature 2715..2720
/label=SDS
misc_feature 2928..2933
/label=SDS
misc_feature 2931..2936
/label=SDS
misc_feature 2949..2954
/label=SDS
misc_feature 2952..2957
/label=SDS
misc_feature 3174..3179
/label=SDS
misc_feature 3177..3182
/label=SDS
misc_feature 3180..3185
/label=SDS
misc_feature 3258..3263
/label=SDS
misc_feature 3261..3266
/label=SDS
misc_feature 3330..3335
/label=SDS
misc_feature 3432..3437
/label=SDS
misc_feature 3645..3650
/label=SDS
misc_feature 3900..3905
/label=SDS
misc_feature 3930..3935
/label=SDS
misc_feature 3933..3938
/label=SDS
misc_feature 4353..4358
/label=SDS
intron 4400..4440
/label=Splicing Donor
misc_feature 4444..4477
/label=lox2272-JT1571
repeat_region 4522..4662
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4522..4651
/label=AAV2 ITR
rep_origin 4737..5192
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5474..5578
/gene="bla"
/label=AmpR promoter
CDS 5579..6439
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6610..7198
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgggcag agcgcacatc gcccacagtc cccgagaagt
181 tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg
241 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
301 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagcgta
361 cgccaccatg tacccttatg acgtacctga ctatgctgga tctgcacagc aagctgccga
421 taagtatctc tatgtggata aaaacttcat caacaatccg ctggcccagg ccgactgggc
481 tgccaagaag ctggtatggg tgccttccga caagagtggc tttgagccag ccagcctcaa
541 ggaggaggtg ggcgaagagg ccatcgtgga gctggtggag aatgggaaga aggtgaaggt
601 gaacaaggat gacatccaga agatgaaccc gcccaagttc tccaaggtgg aggacatggc
661 agagctcacg tgcctcaacg aagcctcggt gctgcacaac ctcaaggagc gttactactc
721 agggctcatc tacacctatt caggcctgtt ctgtgtggtc atcaatcctt acaagaacct
781 gcccatctac tctgaagaga ttgtggaaat gtacaagggc aagaagaggc acgagatgcc
841 ccctcacatc tatgccatca cagacaccgc ctacaggagt atgatgcaag accgagaaga
901 tcaatccatc ttgtgcactg gtgaatctgg agctggcaag acggagaaca ccaagaaggt
961 catccagtat ctggcgtacg tggcgtcctc gcacaagagc aagaaggacc agggcgagct
1021 ggagcggcag ctgctgcagg ccaaccccat cctggaggcc ttcgggaacg ccaagaccgt
1081 gaagaatgac aactcctccc gcttcggcaa attcattcgc atcaactttg atgtcaatgg
1141 ctacattgtt ggagccaaca ttgagactta tcttttggag aaatctcgtg ctatccgcca
1201 agccaaggaa gaacggacct tccacatctt ctattatctc ctgtctgggg ctggagagca
1261 cctgaagacc gatctcctgt tggagccgta caacaaatac cgcttcctgt ccaatggaca
1321 cgtcaccatc cccgggcagc aggacaagga catgttccag gagaccatgg aggccatgag
1381 gattatgggc atcccagaag aggagcaaat gggcctgctg cgggtcatct caggggttct
1441 tcagctcggc aacatcgtct tcaagaagga gcggaacact gaccaggcgt ccatgcccga
1501 caacacagct gcccaaaagg tgtcccatct cttgggtatc aatgtgaccg atttcaccag
1561 aggaatcctc accccgcgca tcaaggtggg acgggattac gtccagaagg cgcagactaa
1621 agagcaggct gactttgcca tcgaggcctt ggccaaggcg acctatgagc ggatgttccg
1681 ctggctggtg ctgcgcatca acaaggctct ggacaagacc aagaggcagg gcgcctcctt
1741 catcgggatc ctggacattg ccggcttcga gatctttgat ctgaactcgt ttgagcagct
1801 gtgcatcaat tacaccaatg agaagctgca gcagctcttc aaccacacca tgttcatcct
1861 ggagcaggag gagtaccagc gcgagggcat cgagtggaac ttcatcgact ttggcctcga
1921 cctgcagccc tgcatcgacc tcattgagaa gccagcaggc cccccgggca ttctggccct
1981 gctggacgag gagtgctggt tccccaaagc caccgacaag agcttcgtgg agaaggtgat
2041 gcaggagcag ggcacccacc ccaagttcca gaagcccaag cagctgaagg acaaagctga
2101 tttctgcatt atccactatg ccggcaaggt ggattacaaa gctgacgagt ggctgatgaa
2161 gaacatggat cccctgaatg acaacatcgc cacactgctc caccagtcct ctgacaagtt
2221 tgtctcggag ctgtggaagg atgtggaccg catcatcggc ctggaccagg tggccggcat
2281 gtcggagacc gcactgcccg gggccttcaa gacgcggaag ggcatgttcc gcactgtggg
2341 gcagctttac aaggagcagc tggccaagct gatggctacg ctgaggaaca cgaaccccaa
2401 ctttgtccgc tgcatcatcc ccaaccacga gaagaaggcc ggcaagctgg acccgcatct
2461 cgtgctggac cagctgcgct gcaacggtgt tctcgagggc atccgtatct gccgccaggg
2521 cttccccaac agggtggtct tccaggagtt tcggcagaga tatgagatcc tgactccaaa
2581 ctccattccc aagggtttca tggacgggaa gcaggcgtgc gtgctcatga taaaagccct
2641 ggagctcgac agcaatctgt accgcattgg ccagagcaaa gtcttcttcc gtgccggtgt
2701 gctggcccac ctggaggagg agcgagacct gaagatcacc gacgtcatca tagggttcca
2761 ggcctgctgc aggggctacc tggccaggaa agcatttgcc aagcggcagc agcagcttac
2821 cgccatgaag gtcctccagc ggaactgcgc tgcctacctg aagctgcgga actggcagtg
2881 gtggcggctc ttcaccaagg tcaagccgct gctgcaggtg agccggcagg aggaggagat
2941 gatggccaag gaggaggagc tggtgaaggt cagagagaag cagctggctg cggagaacag
3001 gctcacggag atggagacgc tgcagtctca gctcatggca gagaaattgc agctgcagga
3061 gcagctccag gcagaaaccg agctgtgtgc cgaggctgag gagctccggg cccgcctgac
3121 cgccaagaag caggaattag aagagatctg ccatgaccta gaggccaggg tggaggagga
3181 ggaggagcgc tgccagcacc tgcaggcgga gaagaagaag atgcagcaga acatccagga
3241 gcttgaggag cagctggagg aggaggagag cgcccggcag aagctgcagc tggagaaggt
3301 gaccaccgag gcgaagctga aaaagctgga ggaggagcag atcatcctgg aggaccagaa
3361 ctgcaagctg gccaaggaaa agaaactgct ggaagacaga atagctgagt tcaccaccaa
3421 cctcacagaa gaggaggaga aatctaagag cctcgccaag ctcaagaaca agcatgaggc
3481 aatgatcact gacttggaag agcgcctccg cagggaggag aagcagcgac aggagctgga
3541 gaagacccgc cggaagctgg agggagactc cacagacctc agcgaccaga tcgccgagct
3601 ccaggcccag atcgcggagc tcaagatgca gctggccaag aaagaggagg agctccaggc
3661 cgccctggcc agagtggaag aggaagctgc ccagaagaac atggccctca agaagatccg
3721 ggagctggaa tctcagatct ctgaactcca ggaagacctg gagtctgagc gtgcttccag
3781 gaataaagct gagaagcaga aacgggacct tggggaagag ctagaggcgc tgaaaacaga
3841 gttggaggac acgctggatt ccacagctgc ccagcaggag ctcaggtcaa aacgtgagca
3901 ggaggtgaac atcctgaaga agaccctgga ggaggaggcc aagacccacg aggcccagat
3961 ccaggagatg aggcagaagc actcacaggc cgtggaggag ctggcggagc agctggagca
4021 gacgaagcgg gtgaaagcaa acctcgagaa ggcaaagcag actctggaga acgagcgggg
4081 ggagctggcc aacgaggtga aggtgctgct gcagggcaaa ggggactcgg agcacaagcg
4141 caagaaagtg gaggcgcagc tgcaggagct gcaggtcaag ttcaacgagg gagagcgcgt
4201 gcgcacagag ctggccgaca aggtcaccaa gctgcaggtg gagctggaca acgtgaccgg
4261 gcttctcagc cagtccgaca gcaagtccag caagctcacc aaggacttct ccgcgctgga
4321 gtcccagctg caggacactc aggagctgct gcaggaggag aaccggcaga agctgagcct
4381 gagcaccaag ctcaagcagg taagtatcaa ggttacaaga caggtttaag gagaccaata
4441 agctattatt cgtataggat actttatacg aagttattaa gttatggaga ccggtgtggc
4501 acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc
4561 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc
4621 gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt
4681 ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc
4741 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
4801 acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
4861 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
4921 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc
4981 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
5041 cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg
5101 gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc
5161 gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc
5221 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
5281 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
5341 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
5401 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
5461 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
5521 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
5581 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
5641 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
5701 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
5761 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
5821 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
5881 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
5941 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
6001 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
6061 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
6121 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
6181 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
6241 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
6301 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
6361 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
6421 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
6481 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
6541 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
6601 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
6661 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
6721 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
6781 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
6841 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
6901 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
6961 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
7021 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
7081 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
7141 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
7201 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
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