| 质粒名称: | pAAVLINK-EF1a-HA-5'-EIF4G1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001629 |
| 产品用途: | 5' AAVLINK plasmid for EIF4G1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | EIF4G1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-EIF4G1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-EIF4G1载体质粒基本信息
pAAVLINK-EF1a-HA-5'-EIF4G1质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-EIF4G1载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-EIF4G1质粒载体简介
pAAVLINK-EF1a-HA-5'-EIF4G1质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-EIF4G1 6786 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6786)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 21, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6786
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..3926
/codon_start=1
/label=EIF4G1
/translation="MNKAPQSTGPPPAPSPGLPQPAFPPGQTAPVVFSTPQATQMNTPS
QPRQHFYPSRAQPPSSAASRVQSAAPARPGPAAHVYPAGSQVMMIPSQISYPASQGAYY
IPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPTGVAPAPV
LMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGE
TPQVAVIVRPDDRSQGAIIADRPGLPGPEHSPSESQPSSPSPTPSPSPVLEPGSEPNLA
VLSIPGDTMTTIQMSVEESTPISRETGEPYRLSPEPTPLAEPILEVEVTLSKPVPESEF
SSSPLQAPTPLASHTVEIHEPNGMVPSEDLEPEVESSPELAPPPACPSESPVPIAPTAQ
PEELLNGAPSPPAVDLSPVSEPEEQAKEVTASVAPPTIPSATPATAPSATSPAQEEEME
EEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPANLSQNLEAAAATQVAVSVPKRRRK
IKELNKKEAVGDLLDAFKEANPAVPEVENQPPAGSNPGPESEGSGVPPRPEEADETWDS
KEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPH
ISDVVLDKANKTPLRPLDPTRLQGINCGPDFTPSFANLGRTTLSTRGPPRGGPGGELPR
GPAGLGPRRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDAD
GSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFS
VAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEA
ATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEE
SLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRGSN
WVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVD
DGGWNTVPISK"
misc_feature 1309..1314
/label=SDS
misc_feature 2025..2030
/label=SDS
misc_feature 2106..2111
/label=SDS
misc_feature 2109..2114
/label=SDS
misc_feature 2182..2187
/label=SDS
misc_feature 2319..2324
/label=SDS
misc_feature 2352..2357
/label=SDS
misc_feature 2390..2395
/label=SDS
misc_feature 3768..3773
/label=SDS
intron 3927..3967
/label=Splicing Donor
misc_feature 3971..4004
/label=lox2272-JT1571
repeat_region 4049..4189
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4049..4178
/label=AAV2 ITR
rep_origin 4264..4719
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5001..5105
/gene="bla"
/label=AmpR promoter
CDS 5106..5966
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6137..6725
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat gaacaaagct ccacagtcca caggcccccc
781 acccgcccca tcccccggac tcccacagcc agcgtttccc ccggggcaga cagcgccggt
841 ggtgttcagt acgccacaag cgacacaaat gaacacgcct tctcagcccc gccagcactt
901 ctaccctagc cgggcccagc ccccgagcag tgcagcctcc cgagtgcaga gtgcagcccc
961 tgcccgccct ggcccagctg cccatgtcta ccctgctgga tcccaagtaa tgatgatccc
1021 ttcccagatc tcctacccag cctcccaggg ggcctactac atccctggac aggggcgttc
1081 cacatacgtt gtcccgacac agcagtaccc tgtgcagcca ggagccccag gcttctatcc
1141 aggtgcaagc cctacagaat ttgggaccta cgctggcgcc tactatccag cccaaggggt
1201 gcagcagttt cccactggcg tggcccccgc cccagttttg atgaaccagc caccccagat
1261 tgctcccaag agggagcgta agacgatccg aattcgagat ccaaaccaag gaggaaagga
1321 tatcacagag gagatcatgt ctggggcccg cactgcctcc acacccaccc ctccccagac
1381 gggaggcggt ctggagcctc aagctaatgg ggagacgccc caggttgctg tcattgtccg
1441 gccagatgac cggtcacagg gagcaatcat tgctgaccgg ccagggctgc ctggcccaga
1501 gcatagccct tcagaatccc agccttcgtc gccttctccg accccatcac catccccagt
1561 cttggaaccg gggtctgagc ctaatctcgc agtcctctct attcctgggg acactatgac
1621 aactatacaa atgtctgtag aagaatcaac ccccatctcc cgtgaaactg gggagccata
1681 tcgcctctct ccagaaccca ctcctctcgc cgaacccata ctggaagtag aagtgacact
1741 tagcaaaccg gttccagaat ctgagttttc ttccagtcct ctccaggctc ccaccccttt
1801 ggcatctcac acagtggaaa ttcatgagcc taatggcatg gtcccatctg aagatctgga
1861 accagaggtg gagtcaagcc cagagcttgc tcctccccca gcttgcccct ccgaatcccc
1921 tgtgcccatt gctccaactg cccaacctga ggaactgctc aacggagccc cctcgccacc
1981 agctgtggac ttaagcccag tcagtgagcc agaggagcag gccaaggagg tgacagcatc
2041 agtggcgccc cccaccatcc cctctgctac tccagctacg gctccttcag ctacttcccc
2101 agctcaggag gaggaaatgg aagaagaaga agaagaggaa gaaggagaag caggagaagc
2161 aggagaagct gagagtgaga aaggaggaga ggaactgctc cccccagaga gtacccctat
2221 tccagccaac ttgtctcaga atttggaggc agcagcagcc actcaagtgg cagtatctgt
2281 gccaaagagg agacggaaaa ttaaggagct aaataagaag gaggctgttg gagaccttct
2341 ggatgccttc aaggaggcga acccggcagt accagaggtg gaaaatcagc ctcctgcagg
2401 cagcaatcca ggcccagagt ctgagggcag tggtgtgccc ccacgtcctg aggaagcaga
2461 tgagacctgg gactcaaagg aagacaaaat tcacaatgct gagaacatcc agcccgggga
2521 acagaagtat gaatataagt cagatcagtg gaagcctcta aacctagagg agaaaaaacg
2581 ttacgaccgt gagttcctgc ttggttttca gttcatcttt gccagtatgc agaagccaga
2641 gggattgcca catatcagtg acgtggtgct ggacaaggcc aataaaacac cactgcggcc
2701 actggatccc actagactac aaggcataaa ttgtggccca gacttcactc catcctttgc
2761 caaccttggc cggacaaccc ttagcacccg tgggccccca aggggtgggc caggtgggga
2821 gctgccccgt gggccggctg gcctgggacc ccggcgctct cagcagggac cccgaaaaga
2881 accacgcaag atcattgcca cagtgttaat gaccgaagat ataaaactga acaaagcaga
2941 gaaagcctgg aaacccagca gcaagcggac ggcggctgat aaggatcgag gggaagaaga
3001 tgctgatggc agcaaaaccc aggacctatt ccgcagggtg cgctccatcc tgaataaact
3061 gacaccccag atgttccagc agctgatgaa gcaagtgacg cagctggcca tcgacaccga
3121 ggaacgcctc aaaggggtca ttgacctcat ttttgagaag gccatttcag agcccaactt
3181 ctctgtggcc tatgccaaca tgtgccgctg cctcatggcg ctgaaagtgc ccactacgga
3241 aaagccaaca gtgactgtga acttccgaaa gctgttgttg aatcgatgtc agaaggagtt
3301 tgagaaagac aaagatgatg atgaggtttt tgagaagaag caaaaagaga tggatgaagc
3361 tgctacggca gaggaacgag gacgcctgaa ggaagagctg gaagaggctc gggacatagc
3421 ccggcggcgc tctttaggga atatcaagtt tattggagag ttgttcaaac tgaagatgtt
3481 aacagaggca ataatgcatg actgtgtggt caaactgctt aagaaccatg atgaagagtc
3541 ccttgagtgc ctttgtcgtc tgctcaccac cattggcaaa gacctggact ttgaaaaagc
3601 caagccccga atggatcagt atttcaacca gatggaaaaa atcattaaag aaaagaagac
3661 gtcatcccgc atccgcttta tgctgcagga cgtgctggat ctgcgaggga gcaattgggt
3721 gccacgccga ggggatcagg gtcccaagac cattgaccag atccataagg aggctgagat
3781 ggaagaacat cgagagcaca tcaaagtgca gcagctcatg gccaagggca gtgacaagcg
3841 tcggggcggt cctccaggcc ctcccatcag ccgtggactt ccccttgtgg atgatggtgg
3901 ctggaacaca gttcccatca gcaaaggtaa gtatcaaggt tacaagacag gtttaaggag
3961 accaataagc tattattcgt ataggatact ttatacgaag ttattaagtt atggagaccg
4021 gtgtggcacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc
4081 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
4141 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
4201 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
4261 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
4321 ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg
4381 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
4441 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
4501 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
4561 gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt
4621 tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat
4681 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
4741 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
4801 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
4861 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
4921 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
4981 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
5041 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
5101 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
5161 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
5221 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
5281 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
5341 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
5401 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
5461 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
5521 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
5581 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
5641 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
5701 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
5761 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
5821 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
5881 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
5941 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
6001 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
6061 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
6121 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
6181 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
6241 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
6301 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
6361 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
6421 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
6481 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
6541 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
6601 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
6661 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
6721 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
6781 acatgt
//
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