pAAVLINK-EF1a-HA-5'-CCDC88C

LOCUS       pAAVLINK-EF1a-HA-5'-CCDC88C              7229 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7229)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        158..700
                     /label=EF1-alpha
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
     promoter        187..398
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             411..679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    505..510
                     /label=SDS
     CDS             716..742
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             749..4369
                     /codon_start=1
                     /label=CCDC88C
                     /translation="MDVTVSELLELFLQSPLVTWVKTFGPFGSGSQDNLTMYMDLVDGI
                     FLNQIMLQIDPRPTNQRINKHVNNDVNLRIQNLTILVRNIKTYYQEVLQQLIVMNLPNV
                     LMIGRDPLSGKSMEEIKKVLLLVLGCAVQCERKEEFIERIKQLDIETQAGIVAHIQEVT
                     HNQENVFDLQWLELPDVAPEELEALSRSMVLRLRRLIDQRDECTELIVDLTQERDYLQA
                     QHPPSPIKSSSADSTPSPTSSLSSEDKQHLAVELADTKARLRRVRQELEDKTEQLVDTR
                     HEVDQLVLELQKVKQENIQLAADARSARAYRDELDSLREKANRVERLELELTRCKEKLH
                     DVDFYKARMEELREDNIILIETKAMLEEQLTAARARGDKVHELEKENLQLKSKLHDLEL
                     DRDTDKKRIEELLEENMVLEIAQKQSMNESAHLGWELEQLSKNADLSDASRKSFVFELN
                     ECASSRILKLEKENQSLQSTIQGLRDASLVLEESGLKCGELEKENHQLSKKIEKLQTQL
                     EREKQSNQDLETLSEELIREKEQLQSDMETLKADKARQIKDLEQEKDHLNRAMWSLRER
                     SQVSSEARMKDVEKENKALHQTVTEANGKLSQLEFEKRQLHRDLEQAKEKGERAEKLER
                     ELQRLQEENGRLARKVTSLETATEKVEALEHESQGLQLENRTLRKSLDTLQNVSLQLEG
                     LERDNKQLDAENLELRRLVETMRFTSTKLAQMERENQQLEREKEELRKNVDLLKALGKK
                     SERLELSYQSVSAENLRLQQSLESSSHKTQTLESELGELEAERQALRRDLEALRLANAQ
                     LEGAEKDRKALEQEVAQLEKDKKLLEKEAKRLWQQVELKDAVLDDSTAKLSAVEKESRA
                     LDKELARCRDAAGKLKELEKDNRDLTKQVTVHARTLTTLREDLVLEKLKSQQLSSELDK
                     LSQELEKVGLNRELLLQEDDSGSDTKYKILEGRNESALKTTLAMKEEKIVLLEAQMEEK
                     ASLNRQLESELQMLKKECETLRQNQGEGQYLQNSFKHPAGKTAASHQGKEAWGPGHKEA
                     TMELLRVKDRAIELERNNAALQAEKQLLKEQLQHLETQNVTFSSQILTLQKQSAFLQEH
                     NTTLQTQTAKLQVENSTLSSQSAALTAQYTLLQNHHTAKETENESLQRQQEQLTAAYEA
                     LLQDHEHLGTLHERQSAEYEALIRQHSCLKTLHRNLELEHK"
     misc_feature    1227..1232
                     /label=SDS
     misc_feature    2670..2675
                     /label=SDS
     misc_feature    2958..2963
                     /label=SDS
     misc_feature    3222..3227
                     /label=SDS
     misc_feature    3264..3269
                     /label=SDS
     misc_feature    3588..3593
                     /label=SDS
     misc_feature    3861..3866
                     /label=SDS
     regulatory      3890..3899
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          4370..4410
                     /label=Splicing Donor
     misc_feature    4414..4447
                     /label=lox2272-JT1571
     repeat_region   4492..4632
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4492..4621
                     /label=AAV2 ITR
     rep_origin      4707..5162
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5444..5548
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5549..6409
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6580..7168
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
      181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
      421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
      481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
      541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
      601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
      661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
      721 ttatgacgta cctgactatg ctggatctat ggacgtgaca gtctcggagc tcctggagct
      781 cttcctgcag agcccgctgg tgacctgggt gaaaactttt ggcccgtttg gaagcggcag
      841 ccaggacaac ctgactatgt acatggattt agtggacggc atctttttga accaaattat
      901 gctgcaaata gatcccaggc ccacaaatca acgcatcaat aagcacgtca acaatgatgt
      961 gaaccttcgc attcagaatt tgaccatctt ggtgagaaac attaagacct actaccagga
     1021 agttctccag cagctgattg taatgaattt gcccaatgtt ttgatgattg gcagagaccc
     1081 actgtctggg aagagcatgg aggaaatcaa gaaggtgctg ctgctggtgc tgggctgtgc
     1141 tgtccagtgt gagaggaaag aggagttcat tgaaagaatc aaacagctgg acattgagac
     1201 ccaggctggc atcgtggccc atatccagga ggtgactcac aaccaagaga acgtgtttga
     1261 cctgcagtgg ctggagctgc ccgacgtggc tccggaggag ctggaggccc tgtcgaggag
     1321 catggtgctc cgcctgcgga ggctcatcga ccagcgggac gagtgcaccg agctgatcgt
     1381 ggacctcact caggaacggg actacctgca ggcacagcat ccacccagcc ccatcaagtc
     1441 ctccagcgcc gactccactc ccagccccac cagcagcctc tctagcgaag acaagcagca
     1501 cctggccgta gagctggccg acaccaaggc caggctgcgg cgcgtcaggc aggagctgga
     1561 ggataagaca gagcagcttg tggacaccag acatgaggtg gaccagctgg tgctggaact
     1621 gcagaaagtt aagcaggaga acatccagct agcggcagac gcccggtctg ctcgtgccta
     1681 tcgagacgag ctggattccc tgcgggagaa ggcgaatcgc gtggagaggc tggagctgga
     1741 gctgacccgc tgcaaggaga agctgcacga cgtggacttc tacaaggccc gcatggagga
     1801 gctgagagaa gataatatca ttttaattga aaccaaggcc atgctggagg agcagctgac
     1861 tgctgctcgg gcccggggcg ataaagtcca tgagctggaa aaggagaacc tgcagctgaa
     1921 atccaagctt cacgacctgg aattggaccg ggacacagat aagaaacgaa ttgaggagct
     1981 gctggaagaa aacatggtcc ttgagattgc acagaagcag agcatgaacg aatctgccca
     2041 ccttggctgg gagctggagc agctgtccaa gaacgcagac ttgtcagacg cctccaggaa
     2101 gtcgtttgtg tttgagctga acgaatgtgc gtccagccgc atcctgaagc tggagaagga
     2161 gaatcagagc ctccagagca ccatccaggg gctgcgggac gcgtccctgg tgttggagga
     2221 gagcggcctc aagtgcgggg agctggagaa ggagaaccac cagctcagca agaagattga
     2281 aaagttacaa acccagctgg agagagaaaa gcagagcaac caagatctgg agaccctcag
     2341 tgaggagctg atcagagaga aggagcagct gcagagtgac atggagaccc tgaaggctga
     2401 caaagccagg cagatcaagg accttgagca ggaaaaggac cacctcaacc gagccatgtg
     2461 gtcgctgcgg gagaggtcgc aggtcagcag tgaggcccgc atgaaagacg tggagaagga
     2521 aaacaaagcc ctccaccaga cggtgacgga ggccaatggc aagctcagcc agttggagtt
     2581 tgagaagcgg cagctgcaca gggacttgga gcaggccaag gagaaggggg agcgggcaga
     2641 gaagctggag agggagctac agcgactcca ggaggagaac gggaggctgg ccaggaaggt
     2701 gacctccctg gagacagcca ccgagaaagt cgaggccctg gagcatgaga gccagggcct
     2761 gcagctggag aaccggactc tgaggaagtc tctggacacc ttgcagaacg tgtccctgca
     2821 gcttgagggc ctggagcgtg acaacaagca gctggacgca gagaacctgg agctgcgcag
     2881 gctggtggag accatgcgct tcaccagcac caagctggca cagatggaga gggagaacca
     2941 gcagctggag cgtgagaagg aggagctgag gaagaacgtg gatctgctca aggcgctggg
     3001 caagaagtca gagcgcctgg agctcagcta ccagagcgtg agcgctgaga acctccggct
     3061 gcagcagagc ctggagagca gcagccacaa gacgcagacc ttggagagtg agctgggcga
     3121 gctggaggct gagcgccagg cgctgcggcg ggacctggag gccctccggc tggccaatgc
     3181 acagttggag ggggccgaga aggacaggaa ggccctggag caggaggtgg cccagctcga
     3241 gaaggataag aagctgctgg agaaggaggc caagcggctg tggcagcagg tggagctcaa
     3301 ggatgcagtc ttggacgata gcactgccaa actgtccgcc gttgagaagg agagccgcgc
     3361 gctggacaag gagctggccc gctgcaggga cgcagccggc aagctgaagg agctggagaa
     3421 ggacaaccgg gacctcacca agcaagtcac cgtgcatgca aggacactga caactctgag
     3481 ggaggacctg gtgctcgaga agctgaagag ccagcagctc agcagtgagc tggacaagct
     3541 gagccaggaa ctggagaagg tcggcctcaa cagggagctg ctgttgcagg aggacgacag
     3601 cggcagtgac acaaaataca agattttgga gggcagaaat gaatcagcat taaaaacaac
     3661 actagccatg aaagaagaaa agattgtgct cttagaagca cagatggaag agaaagcgag
     3721 cctaaatcgc cagttagaga gtgagctgca gatgctaaag aaggagtgtg agaccctcag
     3781 gcagaaccag ggagaggggc agtacttgca gaactctttc aagcaccctg cggggaagac
     3841 agccgccagt caccagggga aggaggcctg ggggcccggc cataaggaag ccaccatgga
     3901 gcttctccga gtgaaggacc gggccatcga gctggagcgg aataatgcag ctctgcaggc
     3961 tgagaagcag ctgctaaagg aacagctgca gcacctggag acccagaacg tgaccttcag
     4021 cagccagatc ttgacactgc agaaacagag cgccttcctg caggagcaca acaccacact
     4081 gcagacccag accgccaagc tgcaggtgga gaactccacg ctgagttccc agagcgcagc
     4141 gctcaccgcg cagtacacgc tgctgcagaa ccaccacacg gccaaggaga cggagaacga
     4201 aagcctgcag aggcagcagg agcaacttac agcggcctac gaggccctgc tgcaggacca
     4261 cgagcacctg ggcacgctgc acgagcggca atcggccgag tacgaggccc tcatccgcca
     4321 gcacagctgc ctaaagacac tgcatcggaa tctggagctg gagcacaagg taagtatcaa
     4381 ggttacaaga caggtttaag gagaccaata agctattatt cgtataggat actttatacg
     4441 aagttattaa gttatggaga ccggtgtggc acgtgcggac cgagcggccg caggaacccc
     4501 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
     4561 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
     4621 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     4681 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
     4741 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt
     4801 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     4861 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     4921 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     4981 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc
     5041 tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct attggttaaa
     5101 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     5161 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     5221 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     5281 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     5341 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     5401 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     5461 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     5521 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     5581 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     5641 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     5701 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     5761 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     5821 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     5881 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     5941 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     6001 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     6061 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     6121 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     6181 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     6241 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     6301 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     6361 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     6421 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     6481 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     6541 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     6601 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     6661 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     6721 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     6781 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     6841 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     6901 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     6961 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     7021 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     7081 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     7141 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     7201 ggccttttgc tggccttttg ctcacatgt
//