| 出品公司: | Promega |
|---|---|
| 载体名称: | pGEM-5Zf(+) |
| 质粒类型: | 克隆载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 3000 bp |
| 5' 测序引物及序列: | M13 fwd |
| 3' 测序引物及序列: | M13 rev |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | 来源于pGEM-3Zf+载体，包含丝状噬菌体f1复制的起源；蓝白筛选 |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | 非病毒 |
买家导航
pGEM5Zf(+)载体基本信息
pGEM5Zf(+)载体图谱质粒图谱和多克隆位点信息
载体图谱.png)
 载体图谱1.png)
pGEM5Zf(+)载体简介
pGEM5Zf(+)载体序列
LOCUS pGEM-5Zf(+) 3000 bp ds-DNA circular SYN 24-6-2015
DEFINITION Vector for standard cloning, efficient in vitro RNA synthesis, and
ssDNA production, with an MCS suitable for use with the
Erase-a-Base(TM) system.
ACCESSION X65308
VERSION .
KEYWORDS pGEM-5Zf(+)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3000)
AUTHORS Promega
TITLE Direct Submission
JOURNAL Exported 2017/2/28 from SnapGene 2.3.2
http://www.snapgene.com
COMMENT
FEATURES Location/Qualifiers
source 1..3000
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
misc_feature 10..113
/note="MCS"
/note="multiple cloning site"
promoter complement(125..143)
/note="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(161..177)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 185..201
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(209..239)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
rep_origin complement(563..1151)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1322..2182)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2183..2287)
/gene="bla"
/note="AmpR promoter"
rep_origin complement(2365..2820)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS complement(2797..165)
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/note="lacZ-alpha"
/translation="MTMITPSYLGDTIEYSSYASNALGALPYGRPAGGRTSDIPRPWRP
GACDVGPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRP
SQQLRSLNGEWTRPVAAH"
primer_bind 2961..2977
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter 2984..2
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggccgcggga tatcactagt
61 gcggccgcct gcaggtcgac catatgggag agctcccaac gcgttggatg catagcttga
121 gtattctata gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt
181 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
241 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
301 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
361 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
421 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
481 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
541 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
601 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
661 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
721 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
781 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
841 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
901 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
961 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
1021 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
1081 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
1141 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
1201 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
1261 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
1321 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
1381 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
1441 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
1501 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
1561 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
1621 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
1681 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
1741 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
1801 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
1861 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
1921 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
1981 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
2041 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
2101 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
2161 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
2221 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
2281 ttccgcgcac atttccccga aaagtgccac ctgatgcggt gtgaaatacc gcacagatgc
2341 gtaaggagaa aataccgcat caggaaattg taagcgttaa tattttgtta aaattcgcgt
2401 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
2461 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
2521 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
2581 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
2641 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
2701 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
2761 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
2821 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
2881 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
2941 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata
//
