pMito-mCardinal

LOCUS       pMito-mCardinal             4774 bp DNA     circular SYN 15-JAN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pMito-iRFP713
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Shcherbakova DM, Verkhusha VV
  TITLE     Near-infrared fluorescent proteins for multicolor in vivo imaging.
  JOURNAL   Nat Methods. 2013 Jun 16. doi: 10.1038/nmeth.2521.
  PUBMED    23770755
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 6, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Jun 16. doi: 10.1038/nmeth.2521."
FEATURES             Location/Qualifiers
     source          1..4774
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             597..683
                     /codon_start=1
                     /label=COX8A
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAKIHSL"
     CDS             705..1415
                     /codon_start=1
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     regulatory      705..708
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1562..1683
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1690..2145)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2172..2276
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2278..2635
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2486..2621
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2670..3464
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3696..3743
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4072..4660
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatgt
      601 ccgtcctgac gccgctgctg ctgcggggct tgacaggctc ggcccggcgg ctcccagtgc
      661 cgcgcgccaa gatccattcg ttgggggatc caccggtcgc caccatggtg agcaagggcg
      721 aggagctgat caaggagaac atgcacatga agctgtacat ggaaggcacc gtgaacaacc
      781 accacttcaa gtgcaccacc gaaggggagg gcaagcccta cgagggcacc cagacccaga
      841 ggattaaggt ggtggaggga ggccccctgc cgttcgcatt cgacatcctg gccacctgct
      901 ttatgtacgg gagcaagacc ttcatcaacc acacccaggg catccccgat ttctttaagc
      961 agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc
     1021 ttaccgttac ccaggacacc agcctccagg acggctgctt gatctacaac gtcaagctca
     1081 gaggggtgaa cttcccatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg
     1141 ccaccaccga gaccctgtac cccgctgacg gcggcctgga aggcagatgc gacatggccc
     1201 tgaagctcgt gggcgggggc cacctgcact gcaacctgaa gaccacatac agatccaaga
     1261 aacccgctaa gaacctcaag atgcccggcg tctactttgt ggaccgcaga ctggaaagaa
     1321 tcaaggaggc cgacaatgag acctacgtcg agcagcacga ggtggctgtg gccagatact
     1381 gcgacctccc tagcaaactg gggcacaaac ttaatggcat ggacgagctg tacaagtaaa
     1441 gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     1501 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     1561 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     1621 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     1681 ttaaggcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     1741 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     1801 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     1861 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     1921 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     1981 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     2041 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     2101 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc
     2161 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     2221 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg
     2281 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     2341 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     2401 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     2461 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     2521 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     2581 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg
     2641 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     2701 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     2761 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     2821 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
     2881 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     2941 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     3001 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     3061 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     3121 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     3181 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     3241 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     3301 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     3361 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     3421 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     3481 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     3541 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     3601 gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg
     3661 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
     3721 gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt
     3781 cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc
     3841 ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc
     3901 tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt
     3961 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     4021 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4081 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4141 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4201 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4261 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4321 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4381 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4441 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4501 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4561 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4621 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4681 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     4741 tgattctgtg gataaccgta ttaccgccat gcat
//