| 质粒名称: | pWPI |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 12254 |
| 存储实验室: | Didier Trono |
|
载体骨架基本信息 |
|
| 载体名称: | pWPXL |
| 载体抗性: | 氨苄西林 |
| 空载体大小: | 11101 bp |
| 载体类型: | 哺乳动物表达，慢病毒，cre/lox |
| 高拷贝/低拷贝: | 高拷贝 |
| 宿生菌： | Stbl3 |
| 生长说明： | 使用stbl3或hb101来减少重组的机会。在37摄氏度下生长 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 标签/融合蛋白: | EMCVIRES-EGFP磁带盒（主干网上的C终端） |
| 克隆方法: | 限制性酶 |
| 5' 克隆位点: | MCS-见地图（未销毁） |
| 3' 克隆位点: | MCS-见地图（未销毁） |
| 5' 测序引物 | See map |
|
文献引用方法 |
|
| 材料和方法部分: | |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pWPI 11103 bp ds-DNA circular SYN 07-AUG-2019
DEFINITION 2nd gen bicistronic lentiviral vector allows for simultaneous
expression of a transgene and EGFP marker to facilitate tracking of
transduced cells..
ACCESSION .
VERSION .
KEYWORDS pWPI
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11103)
TITLE Trono Lab Constitutive Lentiviral Plasmids
REFERENCE 2 (bases 1 to 11103)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 7, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11103
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1299..1532
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
promoter 2144..3322
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 2375..3313
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 3270..3290
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
misc_feature 3370..3487
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
misc_feature 3554..4105
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(3698..3715)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 3925..3944
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 4108..4824
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(4153..4174)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(4414..4433)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 4761..4782
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(4903..4919)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4904..4920)
/label=pBluescriptKS
/note="For pBluescript vector"
misc_feature 4933..5521
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4986..5006)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5404..5415)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
protein_bind 5665..5698
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
LTR 5718..5898
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
promoter complement(5923..5941)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(5924..5941)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(6231..6250)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(6464..6483)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 6583..6605
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(6643..6661)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6729..6833
/gene="bla"
/label=AmpR promoter
CDS 6834..7694
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7052..7071)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7865..8453
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8354..8373
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8607..8624
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(8694..8714)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 8699..9028
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 8879..9014
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 8941..8960
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 10162..10227
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 10357..10377
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 10802..10936
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(10839..10858)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 10893..10912
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagcctcga
2041 ccatcgatgt cgacgataag ctttgcaaag atggataaag ttttaaacag agaggaatct
2101 ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca
2161 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
2221 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
2281 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
2341 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
2401 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
2461 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
2521 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
2581 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
2641 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
2701 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
2761 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
2821 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
2881 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
2941 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
3001 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
3061 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
3121 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
3181 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
3241 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
3301 tcttccattt caggtgtcgt gaggaatttc gacatttaaa tttaattaat ctcgacggta
3361 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
3421 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
3481 aaattttatc gatcacgaga ctagcctcga ggtttaaact acgggctgca ggaattccgc
3541 cccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
3601 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
3661 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
3721 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
3781 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
3841 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
3901 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
3961 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
4021 acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
4081 tcctttgaaa aacacgatga taataccatg gtgagcaagg gcgaggagct gttcaccggg
4141 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
4201 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
4261 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
4321 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
4381 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
4441 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
4501 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
4561 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
4621 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
4681 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
4741 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
4801 ctcggcatgg acgagctgta caagtccgga ctcagatctc gactagctag tagctagcta
4861 gctagtcgag ctcaagcttc gaattcgata tcaagcttat cgcgataccg tcgacctcga
4921 gggaattccg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
4981 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
5041 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
5101 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
5161 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
5221 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
5281 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
5341 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
5401 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
5461 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
5521 catcgggaat tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct
5581 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
5641 agatgggatc aattcaccat gggaataact tcgtatagca tacattatac gaagttatgc
5701 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
5761 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
5821 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
5881 tgtggaaaat ctctagcagc atctagaatt aattccgtgt attctatagt gtcacctaaa
5941 tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat
6001 gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta
6061 aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc
6121 gcacccggaa atggtcagcg aaccaatcag cagggtcatc gctagccaga tcctctacgc
6181 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
6241 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
6301 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
6361 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat
6421 gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg
6481 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
6541 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
6601 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
6661 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
6721 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
6781 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
6841 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
6901 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
6961 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
7021 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
7081 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
7141 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
7201 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
7261 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
7321 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
7381 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
7441 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
7501 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
7561 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
7621 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
7681 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
7741 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
7801 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
7861 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
7921 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
7981 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
8041 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
8101 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
8161 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
8221 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
8281 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
8341 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
8401 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
8461 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
8521 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
8581 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
8641 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt
8701 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
8761 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
8821 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
8881 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
8941 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
9001 cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat
9061 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
9121 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc
9181 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat
9241 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc
9301 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt
9361 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
9421 cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat
9481 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
9541 aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta
9601 tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt
9661 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
9721 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
9781 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
9841 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta
9901 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg
9961 acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg
10021 acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg
10081 ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
10141 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
10201 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag
10261 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
10321 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
10381 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
10441 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
10501 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac
10561 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa
10621 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat
10681 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
10741 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
10801 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
10861 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
10921 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
10981 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
11041 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
11101 agt
//
